LOCUS       Exported                5289 bp DNA     circular SYN 30-NOV-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5289)
  AUTHORS   CS
  TITLE     Direct Submission
  JOURNAL   Exported Dec 7, 2021 from SnapGene Viewer 6.0.0
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5289
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..303
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        304..507
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     5'UTR           597..636
                     /label=HSV TK 5'-UTR
                     /note="5' untranslated region from the herpes simplex virus
                     thymidine kinase gene"
     CDS             672..941
                     /codon_start=1
                     /label=SARS CoV2/RaTG13 E
                     /translation="MYSFVSEETGTLIVNSVLLFLAFVVFLLVTLAILTALRLCAYCCN
                     IVNVSLVKPSFYVYSRVKNLNSSRVPDLLVGKPIPNPLLGLDST"
     CDS             897..938
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     intron          968..1540
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     polyA_site      1737..1792
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal"
     polyA_signal    1737..1792
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     promoter        complement(2129..2325)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      2143..2278
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     rep_origin      complement(2563..3151)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3620..3998)
                     /label=ISS
                     /note="immunostimulatory sequence from the AmpR gene; 
                     contains unmethylated CpG dinucleotides in the context of 
                     5'-AACGTT-3' (Sato et al., 1996)"
     promoter        complement(4193..4297)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(4629..5084)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     5229..5245
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        5252..5270
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     enhancer        join(5289,1..303)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg
       61 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa
      121 tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca
      181 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac
      241 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc
      301 atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga
      361 tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg
      421 gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta
      481 cggtgggagg tctatataag cagagctcgt ttagtgaacc gtcagatcgc ctggagacgc
      541 catccacgct gttttgacct ccatagaaga caccgggacc gatccagcct ccgggggatc
      601 ttggtggcgt gaaactcccg cacctcttcg gccagcgcct tgtagaagcg cgtaggcctt
      661 ctagagccac catgtatagt tttgtctctg aagaaacagg tacattgatt gtgaatagcg
      721 tgcttttgtt tctggccttt gttgtatttc tgcttgtgac cctggcaatc ttgacagcgc
      781 tccggctgtg tgcctattgt tgcaatatcg tgaacgtctc attggttaag ccctcattct
      841 acgtttattc cagagtgaag aatctcaata gctctcgggt gccagatctc ctggtcggca
      901 agcctatacc aaaccctctc cttggccttg atagcaccta gacgcgtcgg atcctgagaa
      961 cttcagggtg agtttgggga cccttgattg ttctttcttt ttcgctattg taaaattcat
     1021 gttatatgga gggggcaaag ttttcagggt gttgtttaga atgggaagat gtcccttgta
     1081 tcaccatgga ccctcatgat aattttgttt ctttcacttt ctactctgtt gacaaccatt
     1141 gtctcctctt attttctttt cattttctgt aactttttcg ttaaacttta gcttgcattt
     1201 gtaacgaatt tttaaattca cttttgttta tttgtcagat tgtaagtact ttctctaatc
     1261 actttttttt caaggcaatc agggtatatt atattgtact tcagcacagt tttagagaac
     1321 aattgttata attaaatgat aaggtagaat atttctgcat ataaattctg gctggcgtgg
     1381 aaatattctt attggtagaa acaactacat cctggtcatc atcctgcctt tctctttatg
     1441 gttacaatga tatacactgt ttgagatgag gataaaatac tctgagtcca aaccgggccc
     1501 ctctgctaac catgttcatg ccttcttctt tttcctacag ctcctgggca acgtgctggt
     1561 tgttgtgctg tctcatcatt ttggcaaaga attcactcct caggtgcagg ctgcctatca
     1621 gaaggtggtg gctggtgtgg ccaatgccct ggctcacaaa taccactgag atctttttcc
     1681 ctctgccaaa aattatgggg acatcatgaa gccccttgag catctgactt ctggctaata
     1741 aaggaaattt attttcattg caatagtgtg ttggaatttt ttgtgtctct cactcggaag
     1801 gacatatggg agggcaaatc atttaaaaca tcagaatgag tatttggttt agagtttggc
     1861 aacatatgcc atatgctggc tgccatgaac aaaggtggct ataaagaggt catcagtata
     1921 tgaaacagcc ccctgctgtc cattccttat tccatagaaa agccttgact tgaggttaga
     1981 ttttttttat attttgtttt gtgttatttt tttctttaac atccctaaaa ttttccttac
     2041 atgttttact agccagattt ttcctcctct cctgactact cccagtcata gctgtccctc
     2101 ttctcttatg aagatcctcg agaagctgtt tgcaaaagcc taggcctcca aaaaagcctc
     2161 ctcactactt ctggaatagc tcagaggccg aggcggcctc ggcctctgca taaataaaaa
     2221 aaattagtca gccatggggc ggagaatggg cggaactggg cggagttagg ggcgggatgg
     2281 gcggagttag gggcgggact atggttgctg actaattgag atgcagctgc attaatgaat
     2341 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
     2401 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     2461 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     2521 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     2581 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     2641 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     2701 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     2761 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     2821 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     2881 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     2941 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     3001 aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     3061 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     3121 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     3181 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     3241 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     3301 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     3361 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
     3421 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
     3481 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     3541 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     3601 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
     3661 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
     3721 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
     3781 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
     3841 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
     3901 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
     3961 tagcagaact ttaaaagtgc tcatcattgg aaaacgttcg aaaacgttct tcggggcgaa
     4021 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
     4081 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
     4141 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
     4201 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
     4261 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
     4321 ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga
     4381 ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc
     4441 cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg
     4501 cgtcagcggg tgttggcggg tgtcggggct ggcttaacta tgcggcatca gagcagattg
     4561 tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc
     4621 gcatcaggaa attgtaaacg ttaatatttt gttaaaattc gcgttaaatt tttgttaaat
     4681 cagctcattt tttaaccaat aggccgaaat cggcaaaatc ccttataaat caaaagaata
     4741 gaccgagata gggttgagtg ttgttccagt ttggaacaag agtccactat taaagaacgt
     4801 ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc
     4861 atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa
     4921 agggagcccc cgatttagag cttgacgggg aaagccggcg aacgtggcga gaaaggaagg
     4981 gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt
     5041 aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc gcgtcgcgcc attcgccatt
     5101 caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct
     5161 ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc
     5221 acgacgttgt aaaacgacgg ccagtgaatt gtaatacgac tcactatagg gcgaattcga
     5281 gctcgcccc
//
