LOCUS       Exported                5730 bp DNA     circular SYN 30-NOV-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5730)
  AUTHORS   CS
  TITLE     Direct Submission
  JOURNAL   Exported Dec 7, 2021 from SnapGene Viewer 6.0.0
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5730
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..303
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        304..507
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     5'UTR           597..636
                     /label=HSV TK 5'-UTR
                     /note="5' untranslated region from the herpes simplex virus
                     thymidine kinase gene"
     regulatory      666..675
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             672..1382
                     /codon_start=1
                     /label=M
                     /translation="MADSNGTITVEELKKLLEQWNLVIGFLFLTWICLLQFAYANRNRF
                     LYIIKLIFLWLLWPVTLACFVLAAVYRINWITGGIAIAMACLVGLMWLSYFIASFRLFA
                     RTRSMWSFNPETNILLNVPLHGTILTRPLLESELVIGAVILRGHLRIAGHHLGRCDIKD
                     LPKEITVATSRTLSYYKLGASQRVAGDSGFAAYSRYRIGNYKLNTDHSSSSDNIALLVQ
                     GKPIPNPLLGLDST"
     CDS             1338..1379
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     intron          1409..1981
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     polyA_site      2178..2233
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal"
     promoter        complement(2570..2766)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      2584..2719
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     rep_origin      complement(3004..3592)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4061..4439)
                     /label=ISS
                     /note="immunostimulatory sequence from the AmpR gene; 
                     contains unmethylated CpG dinucleotides in the context of 
                     5'-AACGTT-3' (Sato et al., 1996)"
     promoter        complement(4634..4738)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(5070..5525)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     5670..5686
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        5693..5711
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg
       61 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa
      121 tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca
      181 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac
      241 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc
      301 atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga
      361 tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg
      421 gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta
      481 cggtgggagg tctatataag cagagctcgt ttagtgaacc gtcagatcgc ctggagacgc
      541 catccacgct gttttgacct ccatagaaga caccgggacc gatccagcct ccgggggatc
      601 ttggtggcgt gaaactcccg cacctcttcg gccagcgcct tgtagaagcg cgtaggcctt
      661 ctagagccac catggcagat tcaaacggga cgatcacagt agaggagctg aagaaactgc
      721 tggagcaatg gaatctggtc ataggcttcc ttttccttac atggatttgt ctgctgcaat
      781 tcgcgtacgc aaaccgcaac cgctttctct atattatcaa acttatattc ctctggttgc
      841 tctggccagt aactctggct tgtttcgttc tggcggccgt gtacaggatt aattggatta
      901 ccggcggaat cgcaatcgca atggcatgcc tggtgggtct gatgtggctg agctacttta
      961 tcgcaagctt cagattgttc gccagaactc gctctatgtg gtctttcaat cctgagacta
     1021 acattctgct gaacgttcct ctgcatggaa ctattctcac ccgccccctg ctcgaatccg
     1081 agctcgtcat cggcgcagta attctgcggg gacatctccg aatcgctgga caccatcttg
     1141 gacgttgtga tatcaaggat ctgccgaaag agattacagt tgcaactagt cgaacactgt
     1201 cctattataa actgggcgcc tctcaacgag tggccggtga tagtgggttc gcagcttact
     1261 ctcgttacag aatcggaaac tataaactta acacggacca cagctcttcc tctgataata
     1321 ttgccctgct ggtgcaagga aaacctattc ccaatccact gctcggattg gactctactt
     1381 agacgcgtcg gatcctgaga acttcagggt gagtttgggg acccttgatt gttctttctt
     1441 tttcgctatt gtaaaattca tgttatatgg agggggcaaa gttttcaggg tgttgtttag
     1501 aatgggaaga tgtcccttgt atcaccatgg accctcatga taattttgtt tctttcactt
     1561 tctactctgt tgacaaccat tgtctcctct tattttcttt tcattttctg taactttttc
     1621 gttaaacttt agcttgcatt tgtaacgaat ttttaaattc acttttgttt atttgtcaga
     1681 ttgtaagtac tttctctaat cacttttttt tcaaggcaat cagggtatat tatattgtac
     1741 ttcagcacag ttttagagaa caattgttat aattaaatga taaggtagaa tatttctgca
     1801 tataaattct ggctggcgtg gaaatattct tattggtaga aacaactaca tcctggtcat
     1861 catcctgcct ttctctttat ggttacaatg atatacactg tttgagatga ggataaaata
     1921 ctctgagtcc aaaccgggcc cctctgctaa ccatgttcat gccttcttct ttttcctaca
     1981 gctcctgggc aacgtgctgg ttgttgtgct gtctcatcat tttggcaaag aattcactcc
     2041 tcaggtgcag gctgcctatc agaaggtggt ggctggtgtg gccaatgccc tggctcacaa
     2101 ataccactga gatctttttc cctctgccaa aaattatggg gacatcatga agccccttga
     2161 gcatctgact tctggctaat aaaggaaatt tattttcatt gcaatagtgt gttggaattt
     2221 tttgtgtctc tcactcggaa ggacatatgg gagggcaaat catttaaaac atcagaatga
     2281 gtatttggtt tagagtttgg caacatatgc catatgctgg ctgccatgaa caaaggtggc
     2341 tataaagagg tcatcagtat atgaaacagc cccctgctgt ccattcctta ttccatagaa
     2401 aagccttgac ttgaggttag atttttttta tattttgttt tgtgttattt ttttctttaa
     2461 catccctaaa attttcctta catgttttac tagccagatt tttcctcctc tcctgactac
     2521 tcccagtcat agctgtccct cttctcttat gaagatcctc gagaagctgt ttgcaaaagc
     2581 ctaggcctcc aaaaaagcct cctcactact tctggaatag ctcagaggcc gaggcggcct
     2641 cggcctctgc ataaataaaa aaaattagtc agccatgggg cggagaatgg gcggaactgg
     2701 gcggagttag gggcgggatg ggcggagtta ggggcgggac tatggttgct gactaattga
     2761 gatgcagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     2821 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     2881 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     2941 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     3001 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     3061 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     3121 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     3181 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     3241 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     3301 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     3361 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     3421 gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt
     3481 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     3541 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     3601 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     3661 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     3721 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     3781 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     3841 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     3901 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     3961 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     4021 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     4081 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     4141 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     4201 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     4261 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     4321 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
     4381 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     4441 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     4501 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     4561 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     4621 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     4681 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     4741 catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct
     4801 ataaaaatag gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa
     4861 acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga
     4921 gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact
     4981 atgcggcatc agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca
     5041 gatgcgtaag gagaaaatac cgcatcagga aattgtaaac gttaatattt tgttaaaatt
     5101 cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat
     5161 cccttataaa tcaaaagaat agaccgagat agggttgagt gttgttccag tttggaacaa
     5221 gagtccacta ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg
     5281 cgatggccca ctacgtgaac catcacccta atcaagtttt ttggggtcga ggtgccgtaa
     5341 agcactaaat cggaacccta aagggagccc ccgatttaga gcttgacggg gaaagccggc
     5401 gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag
     5461 tgtagcggtc acgctgcgcg taaccaccac acccgccgcg cttaatgcgc cgctacaggg
     5521 cgcgtcgcgc cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc
     5581 ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt
     5641 aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgaat tgtaatacga
     5701 ctcactatag ggcgaattcg agctcgcccc
//
