LOCUS       Exported                5961 bp DNA     circular SYN 30-NOV-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5961)
  AUTHORS   CS
  TITLE     Direct Submission
  JOURNAL   Exported Dec 7, 2021 from SnapGene Viewer 6.0.0
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5961
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..303
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        304..507
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     5'UTR           597..636
                     /label=HSV TK 5'-UTR
                     /note="5' untranslated region from the herpes simplex virus
                     thymidine kinase gene"
     CDS             672..1613
                     /codon_start=1
                     /label=RaTG13 NSP16
                     /translation="MSSQAWQPGVAMPNLYKMQRMLLEKCDLQNYGDSATLPKGIMMNV
                     AKYTQLCQYLNTLTLAVPYNMRVIHFGAGSDKGVAPGTAVLRQWLPTGTLLVDSDLNDF
                     VSDADSTLIGDCATVHTANKWDLIISDMYDPKTKNVTKENDSKEGFFTYICGFIQQKLA
                     LGGSVAIKITEHSWNADLYKLMGHFAWWTAFVTNVNASSSEAFLIGCNYLGKPREQIDG
                     YVMHANYIFWRNTNPIQLSSYSLFDMSKFPLKLRGTAVMSLKEGQINDMILSLLSKGRL
                     IIRENNRVVISSDVLVNNGKPIPNPLLGLDST"
     CDS             1569..1610
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     intron          1640..2212
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     polyA_site      2409..2464
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal"
     promoter        complement(2801..2997)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      2815..2950
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     rep_origin      complement(3235..3823)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4292..4670)
                     /label=ISS
                     /note="immunostimulatory sequence from the AmpR gene; 
                     contains unmethylated CpG dinucleotides in the context of 
                     5'-AACGTT-3' (Sato et al., 1996)"
     promoter        complement(4865..4969)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(5301..5756)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     5901..5917
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        5924..5942
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg
       61 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa
      121 tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca
      181 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac
      241 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc
      301 atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga
      361 tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg
      421 gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta
      481 cggtgggagg tctatataag cagagctcgt ttagtgaacc gtcagatcgc ctggagacgc
      541 catccacgct gttttgacct ccatagaaga caccgggacc gatccagcct ccgggggatc
      601 ttggtggcgt gaaactcccg cacctcttcg gccagcgcct tgtagaagcg cgtaggcctt
      661 ctagagccac catgtcttcc caagcctggc agcccggtgt cgcgatgccg aacctgtaca
      721 agatgcagcg aatgctcctg gagaagtgcg atctccagaa ctatggcgat tccgccacgc
      781 ttccgaaggg gattatgatg aatgtcgcta aatatactca actctgtcaa tacctgaata
      841 ctctgacact tgctgtgccc tacaatatgc gggttattca ttttggtgcg ggcagcgata
      901 aaggggtcgc cccgggcaca gcagtgttga gacaatggtt gcctactggt acactcctgg
      961 tcgacagtga tctgaatgat tttgttagtg atgcagactc aacccttatc ggcgattgtg
     1021 ctaccgtaca cacggctaac aaatgggatc tgataattag tgacatgtat gatcctaaaa
     1081 ccaagaatgt caccaaggaa aatgacagta aggaagggtt cttcacatac atctgtgggt
     1141 ttatacaaca aaagctggcc ctgggtggaa gcgtggctat caaaataaca gagcactcat
     1201 ggaacgccga tctttataag ttgatgggac atttcgcttg gtggaccgcg ttcgtcacga
     1261 acgttaatgc atcaagttca gaggccttcc tgataggatg taattatctg gggaaacctc
     1321 gggagcaaat tgatgggtat gtaatgcatg caaactatat cttttggaga aatactaacc
     1381 ccattcaact tagctcttac agcctgtttg acatgtctaa attccctttg aagttgcgtg
     1441 gaaccgctgt gatgagcctg aaagagggcc agattaatga tatgatcttg tcacttctct
     1501 caaaaggaag gcttataatt cgggaaaata atagagtcgt gattagttcc gacgtcctgg
     1561 tgaacaatgg taagccaatt ccaaaccccc tgctcggcct tgatagcact tgaacgcgtc
     1621 ggatcctgag aacttcaggg tgagtttggg gacccttgat tgttctttct ttttcgctat
     1681 tgtaaaattc atgttatatg gagggggcaa agttttcagg gtgttgttta gaatgggaag
     1741 atgtcccttg tatcaccatg gaccctcatg ataattttgt ttctttcact ttctactctg
     1801 ttgacaacca ttgtctcctc ttattttctt ttcattttct gtaacttttt cgttaaactt
     1861 tagcttgcat ttgtaacgaa tttttaaatt cacttttgtt tatttgtcag attgtaagta
     1921 ctttctctaa tcactttttt ttcaaggcaa tcagggtata ttatattgta cttcagcaca
     1981 gttttagaga acaattgtta taattaaatg ataaggtaga atatttctgc atataaattc
     2041 tggctggcgt ggaaatattc ttattggtag aaacaactac atcctggtca tcatcctgcc
     2101 tttctcttta tggttacaat gatatacact gtttgagatg aggataaaat actctgagtc
     2161 caaaccgggc ccctctgcta accatgttca tgccttcttc tttttcctac agctcctggg
     2221 caacgtgctg gttgttgtgc tgtctcatca ttttggcaaa gaattcactc ctcaggtgca
     2281 ggctgcctat cagaaggtgg tggctggtgt ggccaatgcc ctggctcaca aataccactg
     2341 agatcttttt ccctctgcca aaaattatgg ggacatcatg aagccccttg agcatctgac
     2401 ttctggctaa taaaggaaat ttattttcat tgcaatagtg tgttggaatt ttttgtgtct
     2461 ctcactcgga aggacatatg ggagggcaaa tcatttaaaa catcagaatg agtatttggt
     2521 ttagagtttg gcaacatatg ccatatgctg gctgccatga acaaaggtgg ctataaagag
     2581 gtcatcagta tatgaaacag ccccctgctg tccattcctt attccataga aaagccttga
     2641 cttgaggtta gatttttttt atattttgtt ttgtgttatt tttttcttta acatccctaa
     2701 aattttcctt acatgtttta ctagccagat ttttcctcct ctcctgacta ctcccagtca
     2761 tagctgtccc tcttctctta tgaagatcct cgagaagctg tttgcaaaag cctaggcctc
     2821 caaaaaagcc tcctcactac ttctggaata gctcagaggc cgaggcggcc tcggcctctg
     2881 cataaataaa aaaaattagt cagccatggg gcggagaatg ggcggaactg ggcggagtta
     2941 ggggcgggat gggcggagtt aggggcggga ctatggttgc tgactaattg agatgcagct
     3001 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
     3061 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     3121 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     3181 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     3241 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     3301 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     3361 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     3421 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     3481 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     3541 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     3601 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     3661 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     3721 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     3781 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     3841 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     3901 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     3961 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     4021 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     4081 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     4141 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     4201 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     4261 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     4321 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     4381 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     4441 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     4501 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     4561 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
     4621 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cgaaaacgtt
     4681 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     4741 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     4801 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     4861 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
     4921 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
     4981 gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata
     5041 ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac
     5101 acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag
     5161 cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat
     5221 cagagcagat tgtactgaga gtgcaccata tgcggtgtga aataccgcac agatgcgtaa
     5281 ggagaaaata ccgcatcagg aaattgtaaa cgttaatatt ttgttaaaat tcgcgttaaa
     5341 tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa
     5401 atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact
     5461 attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc
     5521 actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa
     5581 tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc
     5641 gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt
     5701 cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtcgcg
     5761 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct
     5821 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg
     5881 gttttcccag tcacgacgtt gtaaaacgac ggccagtgaa ttgtaatacg actcactata
     5941 gggcgaattc gagctcgccc c
//
