LOCUS       Exported                6321 bp DNA     circular SYN 30-NOV-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6321)
  AUTHORS   CS
  TITLE     Direct Submission
  JOURNAL   Exported Dec 7, 2021 from SnapGene Viewer 6.0.0
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6321
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..303
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        304..507
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     5'UTR           597..636
                     /label=HSV TK 5'-UTR
                     /note="5' untranslated region from the herpes simplex virus
                     thymidine kinase gene"
     CDS             672..1973
                     /codon_start=1
                     /label=RaTG13 N
                     /translation="MSDNGPQNQRNAPRITFGGPSDSTGSNQNGERSGARPKQRRPQGL
                     PNNTASWFTALTQHGKEDLKFPRGQGVPINTNSSPDDQIGYYRRATRRIRGGDGKMKDL
                     SPRWYFYYLGTGPEAGLPYGANKDGIIWVATEGALNTPKDHIGTRNPANNAAIVLQLPQ
                     GTTLPKGFYAEGSRGGSQASSRSSSRSRNSSRNSTPGSSRGTSPARMAGNGSDAALALL
                     LLDRLNQLESKMSGKGQQQQSQTVTKKSAAEASKKPRQKRTATKQYNVTQAFGRRGPEQ
                     TQGNFGDQELIRQGTDYKHWPQIAQFAPSASAFFGMSRIGMEVTPSGTWLTYTGAIKLD
                     DKDPNFKDQVILLNKHIDAYKTFPPTEPKKDKKKKADETQALPQRQKKQQTVTLLPAAD
                     LDDFSKQLQQSMSSADSTQAGKPIPNPLLGLDST"
     CDS             1929..1970
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     intron          2000..2572
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     polyA_site      2769..2824
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal"
     promoter        complement(3161..3357)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      3175..3310
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     rep_origin      complement(3595..4183)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4652..5030)
                     /label=ISS
                     /note="immunostimulatory sequence from the AmpR gene; 
                     contains unmethylated CpG dinucleotides in the context of 
                     5'-AACGTT-3' (Sato et al., 1996)"
     promoter        complement(5225..5329)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(5661..6116)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     6261..6277
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        6284..6302
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg
       61 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa
      121 tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca
      181 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac
      241 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc
      301 atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga
      361 tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg
      421 gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta
      481 cggtgggagg tctatataag cagagctcgt ttagtgaacc gtcagatcgc ctggagacgc
      541 catccacgct gttttgacct ccatagaaga caccgggacc gatccagcct ccgggggatc
      601 ttggtggcgt gaaactcccg cacctcttcg gccagcgcct tgtagaagcg cgtaggcctt
      661 ctagagccac catgtctgac aatggtcccc agaatcagcg aaatgctccc cggatcacct
      721 ttggtgggcc ctctgacagc accggaagta atcaaaatgg agagcggagt ggtgccaggc
      781 ctaagcaaag gagacctcaa gggctgccga acaatacagc atcctggttt acagctttga
      841 ctcaacatgg caaagaagac ttgaaatttc ctcgaggcca gggagtgcca ataaacacca
      901 attcctctcc tgacgaccaa atcggatatt ataggcgcgc cactcgcaga atccggggcg
      961 gagatgggaa gatgaaagac ctctctccac gttggtattt ctattatctg ggaactggcc
     1021 ctgaagccgg gctcccttac ggggctaata aagacgggat catctgggta gctacagaag
     1081 gggcacttaa cacccctaaa gatcacatag gcactaggaa tcccgcgaac aatgctgcga
     1141 tagtgcttca gcttcctcaa ggaacaactc tccccaaagg attttacgcc gagggtagca
     1201 gaggcggtag tcaagccagt tcaaggagct ctagccgctc ccgcaactca agtcgtaact
     1261 ccacgccagg tagctctcgg ggcacaagcc cggcacgaat ggcgggaaac ggaagcgatg
     1321 ctgcactcgc cttgctgctc ctggaccgcc tgaaccaact ggaatctaag atgtccggga
     1381 aaggacaaca acaacaatct cagacagtga ccaagaagag tgctgctgaa gcgagtaaga
     1441 agccgagaca gaaacggacc gcaacaaagc agtataacgt aacccaagca ttcggccgcc
     1501 gcggccctga acaaactcag ggcaacttcg gcgaccaaga actcattcgg cagggaaccg
     1561 actataagca ttggccgcag attgcacagt ttgcgccgag cgcgtccgcc ttctttggga
     1621 tgtctcggat cggaatggaa gtgaccccgt ccggtacgtg gctgacctat acaggagcga
     1681 taaaactcga cgacaaggac cctaatttta aggatcaagt aattttgctg aataaacaca
     1741 tagacgcata caagacattt ccccctacgg aacctaagaa ggacaagaag aagaaagccg
     1801 atgagactca agctctgcca caacgccaaa agaaacaaca aaccgttacg ctcctgcctg
     1861 ccgctgatct tgatgacttt agtaagcaac tccaacaatc catgagtagc gcggacagca
     1921 ctcaggccgg aaaaccaatt cctaatcctc tcctcggcct cgatagcacg taaacgcgtc
     1981 ggatcctgag aacttcaggg tgagtttggg gacccttgat tgttctttct ttttcgctat
     2041 tgtaaaattc atgttatatg gagggggcaa agttttcagg gtgttgttta gaatgggaag
     2101 atgtcccttg tatcaccatg gaccctcatg ataattttgt ttctttcact ttctactctg
     2161 ttgacaacca ttgtctcctc ttattttctt ttcattttct gtaacttttt cgttaaactt
     2221 tagcttgcat ttgtaacgaa tttttaaatt cacttttgtt tatttgtcag attgtaagta
     2281 ctttctctaa tcactttttt ttcaaggcaa tcagggtata ttatattgta cttcagcaca
     2341 gttttagaga acaattgtta taattaaatg ataaggtaga atatttctgc atataaattc
     2401 tggctggcgt ggaaatattc ttattggtag aaacaactac atcctggtca tcatcctgcc
     2461 tttctcttta tggttacaat gatatacact gtttgagatg aggataaaat actctgagtc
     2521 caaaccgggc ccctctgcta accatgttca tgccttcttc tttttcctac agctcctggg
     2581 caacgtgctg gttgttgtgc tgtctcatca ttttggcaaa gaattcactc ctcaggtgca
     2641 ggctgcctat cagaaggtgg tggctggtgt ggccaatgcc ctggctcaca aataccactg
     2701 agatcttttt ccctctgcca aaaattatgg ggacatcatg aagccccttg agcatctgac
     2761 ttctggctaa taaaggaaat ttattttcat tgcaatagtg tgttggaatt ttttgtgtct
     2821 ctcactcgga aggacatatg ggagggcaaa tcatttaaaa catcagaatg agtatttggt
     2881 ttagagtttg gcaacatatg ccatatgctg gctgccatga acaaaggtgg ctataaagag
     2941 gtcatcagta tatgaaacag ccccctgctg tccattcctt attccataga aaagccttga
     3001 cttgaggtta gatttttttt atattttgtt ttgtgttatt tttttcttta acatccctaa
     3061 aattttcctt acatgtttta ctagccagat ttttcctcct ctcctgacta ctcccagtca
     3121 tagctgtccc tcttctctta tgaagatcct cgagaagctg tttgcaaaag cctaggcctc
     3181 caaaaaagcc tcctcactac ttctggaata gctcagaggc cgaggcggcc tcggcctctg
     3241 cataaataaa aaaaattagt cagccatggg gcggagaatg ggcggaactg ggcggagtta
     3301 ggggcgggat gggcggagtt aggggcggga ctatggttgc tgactaattg agatgcagct
     3361 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
     3421 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     3481 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     3541 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     3601 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     3661 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     3721 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     3781 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     3841 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     3901 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     3961 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     4021 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     4081 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     4141 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     4201 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     4261 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     4321 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     4381 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     4441 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     4501 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     4561 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     4621 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     4681 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     4741 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     4801 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     4861 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     4921 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
     4981 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cgaaaacgtt
     5041 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     5101 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     5161 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     5221 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
     5281 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
     5341 gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata
     5401 ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac
     5461 acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag
     5521 cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat
     5581 cagagcagat tgtactgaga gtgcaccata tgcggtgtga aataccgcac agatgcgtaa
     5641 ggagaaaata ccgcatcagg aaattgtaaa cgttaatatt ttgttaaaat tcgcgttaaa
     5701 tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa
     5761 atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact
     5821 attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc
     5881 actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa
     5941 tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc
     6001 gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt
     6061 cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtcgcg
     6121 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct
     6181 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg
     6241 gttttcccag tcacgacgtt gtaaaacgac ggccagtgaa ttgtaatacg actcactata
     6301 gggcgaattc gagctcgccc c
//
