LOCUS       Exported                9826 bp DNA     circular SYN 07-DEC-2021
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9826)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Dec 7, 2021 from SnapGene Viewer 6.0.0
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9826
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..241
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA"
                     /note="color: #008000; direction: RIGHT"
     misc_RNA        268..343
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
                     /note="color: #00ccff; direction: RIGHT"
     misc_feature    441..1234
                     /label=CBh
                     /note="color: #ff6600; direction: RIGHT"
     enhancer        441..726
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer; 
                     contains an 18-bp deletion relative to the standard CMV 
                     enhancer"
                     /note="color: #ffffff"
     promoter        728..1005
                     /label=chicken beta-actin promoter
                     /note="color: #ffffff; direction: RIGHT"
     intron          1006..1234
                     /label=hybrid intron
                     /note="hybrid between chicken beta-actin (CBA) and minute 
                     virus of mice (MMV) introns (Gray et al., 2011)"
                     /note="color: #------"
     CDS             1252..1254
                     /codon_start=1
                     /label=M
                     /note="color: #993366"
                     /translation="M"
     CDS             1288..1308
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /note="color: #993366"
                     /translation="PKKKRKV"
     misc_feature    1807..1905
                     /label=LINKER
                     /note="color: #ff9900"
     CDS             1906..5145
                     /codon_start=1
                     /label=dSiCas12i(D1049A)
                     /note="color: #33cccc"
                     /translation="MSSDVVRPYNTKLLPDNRKHNMFLQTFKRLNSISLNHFDLLICLY
                     AAITNKKAEEYKSEKEAHVTADSLCAINWFRPMSKRYSKYATTTFNMLELFKEYSGHEP
                     DAYSKNYLMSNIDSDRFVWVDCRKFAKDFAYQMELGFHEFTVLAETLLANSILVLNEST
                     KANWAWGTVSALYGGGDKEDSTLKSKILLAFVDALNNHELKTKREILNQVCESLKYQSY
                     QDMYVDFRSVVDENGNKKSPNGSMPIVTKFETDDLISDNQRKAMISNFTKNAAAKAAKK
                     PIPYLDRLKEHMVSLCDEYNVYAWAAAITNSNADVTARNTRNLTFIGEQNSRRKELSVL
                     QTTTNEKAKDILNKINDNLIQEVRYTPAPKHLGRDLANLFDTLKEKDINNIENEEEKQN
                     VINDCIEQYVDDCRSLNRNPIAALLKHISRYYEDFSAKNFLDGAKLNVLTEVVNRQKAH
                     PTIWSEKAYTWISKFDKNRRQANSSLVGWVVPPEEVHKEKIAGQQSMMWVTLTLLDDGK
                     WVKHHIPFSDSRYYSEVYAYNPNLPYLDGGIPRQSKFGNKPTTNLTAESQALLANSKYK
                     KANKSFLRAKENATHNVRVSPNTSLCIRLLKDSAGNQMFDKIGNVLFGMQINHKITVGK
                     PNYKIEVGDRFLGFDQNQSENHTYAVLQRVSESSHDTHHFNGWDVKVLEKGKVTSDVIV
                     RDEVYDQLSYEGVPYDSSKFAEWRDKRRRFVLENLSIQLEEGKTFLTEFDKLNKDSLYR
                     WNMNYLKLLRKAIRAGGKEFAKIAKTEIFELAVERFGPINLGSLSQISLKMIASFKGVV
                     QSYFSVSGCVDDASKKAHDSMLFTFMCAAEEKRTNKREEKTNRAASFILQKAYLHGCKM
                     IVCEDDLPVADGKTGKAQNADRMDWCARALAKKVNDGCVAMSICYRAIPAYMSSHQDPF
                     VHMQDKKTSVLRPRFMEVNKDSIRDYHVAGLRRMLNSKSDAGTSVYYRQAALHFCEALG
                     VSPELVKNKKTHAAELGKHMGSAMLMPWRGGRVYIASKKLTSDAKSVKYCGEDMWQYHA
                     AEIAAVNIAMYEVCCQTGAFGKKQKKSDELPG"
     CDS             5146..5193
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /note="color: #993366"
                     /translation="KRPAATKKAGQAKKKK"
     polyA_signal    5227..5434
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
                     /note="color: #a6acb3"
     enhancer        5503..5806
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
                     /note="color: #ffffff"
     promoter        5807..6010
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
                     /note="color: #ffffff; direction: RIGHT"
     CDS             6039..6749
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /note="color: #ff0000"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     rep_origin      7304..7759
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
     promoter        8041..8145
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: RIGHT"
     CDS             8146..9006
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 8146 .. 8214 / #ccffcc / signal sequence
                        2: 8215 .. 9006 / #ccffcc
                     Cleavage site after base 8214"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      9177..9765
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
ORIGIN
        1 gagggcctat ttcccatgat tccttcatat ttgcatatac gatacaaggc tgttagagag
       61 ataattggaa ttaatttgac tgtaaacaca aagatattag tacaaaatac gtgacgtaga
      121 aagtaataat ttcttgggta gtttgcagtt ttaaaattat gttttaaaat ggactatcat
      181 atgcttaccg taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga
      241 cgaaacaccg ggtcttcgag aagacctgtt ttagagctag aaatagcaag ttaaaataag
      301 gctagtccgt tatcaacttg aaaaagtggc accgagtcgg tgcttttttt gttttagagc
      361 tagaaatagc aagttaaaat aaggctagtc cgtttttagc gcgtgcgcca attctgcaga
      421 caaatggctc tagaggtacc cgttacataa cttacggtaa atggcccgcc tggctgaccg
      481 cccaacgacc cccgcccatt gacgtcaata gtaacgccaa tagggacttt ccattgacgt
      541 caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg
      601 ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttgtgcccag
      661 tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt
      721 accatggtcg aggtgagccc cacgttctgc ttcactctcc ccatctcccc cccctcccca
      781 cccccaattt tgtatttatt tattttttaa ttattttgtg cagcgatggg ggcggggggg
      841 gggggggggc gcgcgccagg cggggcgggg cggggcgagg ggcggggcgg ggcgaggcgg
      901 agaggtgcgg cggcagccaa tcagagcggc gcgctccgaa agtttccttt tatggcgagg
      961 cggcggcggc ggcggcccta taaaaagcga agcgcgcggc gggcgggagt cgctgcgacg
     1021 ctgccttcgc cccgtgcccc gctccgccgc cgcctcgcgc cgcccgcccc ggctctgact
     1081 gaccgcgtta ctcccacagg tgagcgggcg ggacggccct tctcctccgg gctgtaatta
     1141 gctgagcaag aggtaagggt ttaagggatg gttggttggt ggggtattaa tgtttaatta
     1201 cctggagcac ctgcctgaaa tcactttttt tcaggttgga ccggtgccac catgaaacgg
     1261 acagccgacg gaagcgagtt cgagtcacca aagaagaagc ggaaagtctc tgaggtggag
     1321 ttttcccacg agtactggat gagacatgcc ctgaccctgg ccaagagggc acgggatgag
     1381 agggaggtgc ctgtgggagc cgtgctggtg ctgaacaata gagtgatcgg cgagggctgg
     1441 aacagagcca tcggcctaca cgacccaaca gcccatgccg aaattatggc cctgagacaa
     1501 ggcggcctgg tcatgcagaa ctacagactg attgacgcca ccctgtacgt gacattcgag
     1561 ccttgcgtga tgtgcgctgg cgccatgatc cactctagga tcggccgcgt ggtgtttgga
     1621 tggagaaatt ctaaaagagg cgccgcaggc tccctgatga acgtgctgaa ctaccccggc
     1681 atgaatcacc gcgtcgaaat taccgaggga atcctggcag atgaatgtgc cgccctgctg
     1741 tgcgacttct atcggatgcc tagacaggtg ttcaatgctc agaagaaggc ccagagttcc
     1801 atcaactccg gaggatctag cggaggctcc tctggcagcg agacaccagg aacaagcgag
     1861 tcagcaacac cagagagcag tggcggcagc agcggcggca gcagtatgag ttctgatgtg
     1921 gtgcggcctt ataacacaaa gctgctccca gataacagaa agcacaatat gttcctgcag
     1981 accttcaagc ggctgaacag catctctctg aaccacttcg acctgctgat ctgcctgtac
     2041 gctgcaatca ccaacaagaa ggccgaggaa tacaagtctg aaaaggaagc ccacgtgacc
     2101 gccgatagcc tgtgtgccat caattggttc agacccatga gcaagagata cagcaaatac
     2161 gccaccacca ccttcaacat gttagaactg tttaaggagt acagcggcca cgagcctgat
     2221 gcctattcca agaactacct gatgagcaat atcgacagcg acagattcgt gtgggtggat
     2281 tgtaggaagt tcgctaagga ctttgcctat cagatggaac tgggtttcca cgagttcacc
     2341 gtgttggccg aaaccctgct ggctaattct atcctggtgc tgaacgagag caccaaggcc
     2401 aattgggctt ggggaaccgt gtctgccctg tacggcggcg gagataagga ggacagcaca
     2461 ctgaagagca agattctgct ggccttcgtg gacgccctga acaaccacga gctgaaaaca
     2521 aagagagaaa tcttgaatca agtgtgtgaa tctctgaaat accagagcta ccaggacatg
     2581 tacgtggatt ttagaagcgt ggttgacgaa aacggcaaca agaagtctcc taacggctct
     2641 atgcctatcg tgaccaagtt cgagacagac gacctgatca gcgacaacca aagaaaggcc
     2701 atgatcagca acttcactaa gaacgccgct gccaaggcag ctaagaaacc tatcccttac
     2761 ttggaccgcc tgaaggagca catggtgtcc ctgtgcgacg agtacaatgt gtatgcctgg
     2821 gccgcggcca tcacaaacag caacgccgac gtgaccgccc ggaataccag aaacctgaca
     2881 ttcatcggcg aacagaacag cagacgaaag gaactgagcg tgctgcagac aacaaccaac
     2941 gagaaggcta aggacatcct gaacaagatc aacgacaacc tgattcagga ggtgcggtac
     3001 acccctgccc ctaagcacct gggcagagat ctggccaacc tgtttgatac actgaaggaa
     3061 aaggacatca acaacatcga gaacgaagaa gagaaacaga acgtgatcaa tgactgtatc
     3121 gagcagtacg tggacgattg cagaagcctc aaccggaacc ccatcgcagc cctcctgaag
     3181 cacatctcta ggtactacga ggatttcagc gccaagaatt tcctggacgg cgccaagctg
     3241 aacgtgctga ctgaggtggt gaaccggcag aaggcccacc ccaccatctg gagcgagaag
     3301 gcttacacct ggatcagcaa gttcgacaag aaccggagac aggccaacag cagcctggtc
     3361 ggatgggttg tgccccccga ggaggtgcac aaggagaaaa tcgccggaca gcagagcatg
     3421 atgtgggtga ccctcaccct gctggacgac ggcaagtggg tcaaacatca catccccttc
     3481 agcgacagca gatactacag cgaagtgtac gcctacaacc ctaatctgcc ttatctggac
     3541 ggaggcatcc caagacagag caagttcggc aacaaaccaa caaccaacct gacagccgag
     3601 tcccaggccc tcctggctaa ttctaagtac aagaaagcca acaagagctt cctgcgggct
     3661 aaagagaatg ccacacacaa cgtgcgggtg tcccctaaca cctctctgtg cattagactg
     3721 ctgaaggaca gcgccggaaa ccagatgttc gacaaaatcg gcaacgtgct cttcggcatg
     3781 cagatcaacc acaagatcac cgtgggaaaa cctaactaca agatcgaggt gggcgacaga
     3841 ttcctgggct tcgatcagaa ccagagcgag aaccacacct acgccgtgct gcagagagtg
     3901 tccgagagca gtcacgacac ccaccacttt aacggctggg acgtgaaggt gctggaaaag
     3961 ggcaaagtga ccagcgatgt gatcgtgcgg gacgaggtct acgaccaact gtcttacgag
     4021 ggcgtcccct acgatagcag caagttcgcc gagtggcggg acaagcgcag aagatttgtg
     4081 cttgagaacc tgagcatcca gctggaagag ggcaagacct tcctgacaga gttcgacaag
     4141 ctgaataagg acagcctgta ccgctggaac atgaactacc tgaaactgct gagaaaggcc
     4201 atccgggccg gaggcaaaga gttcgccaag atcgctaaga cagagatctt cgagctggcg
     4261 gtggaaagat tcggccctat taacctgggc agcctgtccc agatcagcct taagatgatt
     4321 gcctccttta agggcgtggt ccagtcctac ttctccgtga gcggctgcgt ggatgatgcc
     4381 tccaaaaagg cccatgattc tatgctgttc acatttatgt gcgccgccga agaaaagcgg
     4441 accaacaaga gagaagaaaa gaccaacaga gccgccagct ttatcctgca aaaagcctac
     4501 ctgcatggct gcaagatgat cgtgtgcgag gacgaccttc ctgtggccga cggcaagaca
     4561 ggcaaagccc agaatgccga ccggatggac tggtgcgcca gagccctggc caagaaggtg
     4621 aacgacggct gtgttgccat gagcatctgc tacagagcta tccctgccta catgagcagc
     4681 caccaggacc cctttgtgca catgcaggat aagaaaacca gcgtgctgcg gcctagattc
     4741 atggaagtta ataaggatag catcagagac taccacgtgg cgggcctgag aagaatgctg
     4801 aacagcaaga gtgacgctgg caccagtgtt tattaccggc aagctgccct gcatttctgc
     4861 gaagccctgg gcgtgagccc tgaactggtg aaaaacaaga aaacccacgc cgccgaactg
     4921 ggcaagcaca tgggcagcgc tatgctgatg ccctggagag gcggtagagt gtacatcgcc
     4981 agcaaaaagc tgacctccga tgccaaatca gtgaagtact gcggcgagga tatgtggcag
     5041 taccacgccg ccgagatcgc cgctgttaac atcgccatgt atgaggtgtg ctgccagacc
     5101 ggcgctttcg gaaagaaaca gaaaaaatcg gacgagctgc ctggaaaaag gccggcggcc
     5161 acgaagaagg ccggccaggc aaagaagaag aagtaagaat tcctagagct cgctgatcag
     5221 cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct
     5281 tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc
     5341 attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg
     5401 aggattggga agagaatagc aggcatgctg gggagcggcc gctagttatt aatagtaatc
     5461 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
     5521 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
     5581 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
     5641 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
     5701 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
     5761 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg
     5821 gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc
     5881 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg
     5941 taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat
     6001 aagcagagct ggtttagtga accgtcagat ccgctagcat ggtgagcaag ggcgaggagg
     6061 ataacatggc catcatcaag gagttcatgc gcttcaaggt gcacatggag ggctccgtga
     6121 acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag ggcacccaga
     6181 ccgccaagct gaaggtgacc aagggtggcc ccctgccctt cgcctgggac atcctgtccc
     6241 ctcagttcat gtacggctcc aaggcctacg tgaagcaccc cgccgacatc cccgactact
     6301 tgaagctgtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg
     6361 gcgtggtgac cgtgacccag gactcctccc tgcaggacgg cgagttcatc tacaaggtga
     6421 agctgcgcgg caccaacttc ccctccgacg gccccgtaat gcagaagaaa accatgggct
     6481 gggaggcctc ctccgagcgg atgtaccccg aggacggcgc cctgaagggc gagatcaagc
     6541 agaggctgaa gctgaaggac ggcggccact acgacgctga ggtcaagacc acctacaagg
     6601 ccaagaagcc cgtgcagctg cccggcgcct acaacgtcaa catcaagttg gacatcacct
     6661 cccacaacga ggactacacc atcgtggaac agtacgaacg cgccgagggc cgccactcca
     6721 ccggcggcat ggacgagctg tacaagtaag gatccgggat gcagaaattg atgatctatt
     6781 aaacaataaa gatgtccact aaaatggaag tttttcctgt catactttgt taagaagggt
     6841 gagaacagag tacctacatt ttgaatggaa ggattggagc tacgggggtg ggggtggggt
     6901 gggattagat aaatgcctgc tctttactga aggctcttta ctattgcttt atgataatgt
     6961 ttcatagttg gatatcataa tttaaacaag caaaaccaaa ttaagggcca gctcattcct
     7021 cccactcatg atctatagat ctatagatct ctcgtgggat cattgttttt ctcttgattc
     7081 ccactttgtg gttctaagta ctgtggtttc caaatgtgtc agtttcatag cctgaagaac
     7141 gagatcagca gcctctgttc cacatacact tcattctcag tattgttttg ccaagttcta
     7201 attccatcag aagctggtcg acctgcaggg gcgcctgatg cggtattttc tccttacgca
     7261 tctgtgcggt atttcacacc gcatacgtca aagcaaccat agtacgcgcc ctgtagcggc
     7321 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
     7381 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
     7441 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
     7501 gaccccaaaa aacttgattt gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
     7561 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
     7621 ggaacaacac tcaaccctat ctcgggctat tcttttgatt tataagggat tttgccgatt
     7681 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa
     7741 atattaacgt ttacaatttt atggtgcact ctcagtacaa tctgctctga tgccgcatag
     7801 ttaagccagc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc
     7861 ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt
     7921 tcaccgtcat caccgaaacg cgcgagacga aagggcctcg tgatacgcct atttttatag
     7981 gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg
     8041 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
     8101 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
     8161 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
     8221 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
     8281 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
     8341 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg
     8401 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
     8461 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
     8521 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
     8581 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
     8641 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca
     8701 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
     8761 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
     8821 ggctggttta ttgctgataa atctggagcc ggtgagcgtg gaagccgcgg tatcattgca
     8881 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
     8941 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
     9001 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
     9061 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     9121 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     9181 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     9241 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     9301 agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag
     9361 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     9421 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     9481 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     9541 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     9601 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     9661 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     9721 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     9781 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgt
//
