LOCUS       pKB233.4                4356 bp ds-DNA     circular     10-JAN-2022
DEFINITION  .
FEATURES             Location/Qualifiers
     rep_origin      238..1189
                     /label="pSC101* (-)"
     promoter        1210..1228
                     /label="pSC101* promoter (-)"
     primer          complement(1493..1512)
                     /label="BL261"
                     /note="sequence: aaacagcgtttgcgacatcc"
     misc_feature    1572..1939
                     /label="par region"
     misc_feature    complement(1945..2050)
                     /label="T0"
     CDS             complement(2079..2741)
                     /label="AmpR"
     CDS             complement(2079..2741)
                     /label="Translation 2741-2079"
     misc_feature    2217..2220
                     /label="BsaI out"
     primer          complement(2303..2322)
                     /label="BL234"
                     /note="sequence: AATAGACTGGATGGAGGCGG"
     primer          2960..2985
                     /label="PR57"
                     /note="sequence: gaagcatttatcagggttattgtctc"
     primer          3066..3098
                     /label="SR224"
                     /note="sequence: GCATTAGGTCTCGCGTCTAAGAAACCATTATTATCATGACATTAAC"
     primer          complement(3087..3113)
                     /label="JS0551"
                     /note="sequence: cgcctatttttataggttaatgtcatg"
     promoter        3132..3201
                     /label="PT0031"
     primer          complement(3172..3190)
                     /label="JS0288"
                     /note="sequence: CCGTAGGCGTCGGAGATTC"
     primer          complement(3188..3202)
                     /label="MH126"
                     /note="sequence: AGGTCTCAtccgacgtcggccg"
     primer          3202..3214
                     /label="MH130"
                     /note="sequence: AGGTCTCAcggaccgtgtcgtccac"
     misc_feature    3202..3391
                     /label="Salmonella slyB Promoter"
     misc_feature    3251..3267
                     /label="PhoP Box"
     misc_feature    3256..3261
                     /label="-35 Box"
     misc_feature    3264..3271
                     /label="arcA Box"
     misc_feature    3280..3285
                     /label="-10 Box"
     misc_feature    3292..3292
                     /label="+1"
     primer          complement(3375..3391)
                     /label="MH131"
                     /note="sequence: AGGTCTCACTTTttacccatctcctgttc"
     misc_feature    3392..3409
                     /label="BBa_B0034"
     primer          3392..3418
                     /label="MH127"
                     /note="sequence: AGGTCTCAAAAGAGGAGAAATACTAGATGGTTTCG"
     primer          3410..3430
                     /label="RB140"
                     /note="sequence: aggtctctTCTAATGGTTTCGAAAGGTGAGGAG"
     primer          3410..3431
                     /label="KB625"
                     /note="sequence: aggtctcaATGGTTTCGAAAGGTGAGGAGG"
     misc_feature    3410..4120
                     /label="mNeonGreen"
     CDS             3410..4120
                     /label="Translation 3410-4120"
     primer          3862..3888
                     /label="kg904"
                     /note="sequence: CAAAAAGACGTATCCCAATGATAAAAC"
     primer          complement(4097..4120)
                     /label="RB141"
                     /note="sequence: aggtctctCTGGTCATTTGTACAGTTCATCCATGCC"
     primer          4121..4144
                     /label="FE390"
                     /note="sequence: AGGTCTCGCCAGGCATCAAATAAAACGAAAGG"
     primer          4121..4144
                     /label="FE449"
                     /note="sequence: AGGTCTCGCCAGGCATCAAATAAAACGAAAGG"
     primer          4121..4144
                     /label="CF075"
                     /note="sequence: AGGTCTCTCCAGGCATCAAATAAAACGAAAGG"
     misc_feature    4121..4249
                     /label="Terminator B0015"
     primer          complement(4227..4250)
                     /label="KB416"
                     /note="sequence: aggtctcattgcTATAAACGCAGAAAGGCCCACCC"
     primer          complement(4227..4253)
                     /label="KB340"
                     /note="sequence: aggtctcaaaacTATAAACGCAGAAAGGCCCACCC"
     primer          4250..4269
                     /label="KB339"
                     /note="sequence: aggtctcaGCAAGGTGTCACCACCCTGC"
     primer          complement(4323..4339)
                     /label="SR642"
                     /note="sequence: GATCAGAAGACCCAGCGCAGCGAGTCAGTG"
ORIGIN
        1 CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATCTCGAGGT TACATTGTCG ATCTGTTCAT
       61 GGTGAACAGC TTTAAATGCA CCAAAAACTC GTAAAAGCTC TGATGTATCT ATCTTTTTTA
      121 CACCGTTTTC ATCTGTGCAT ATGGACAGTT TTCCCTTTGA TATCTAACGG TGAACAGTTG
      181 TTCTACTTTT GTTTGTTAGT CTTGATGCTT CACTGATAGA TACAAGAGCC ATAAGAACCT
      241 CAGATCCTTC CGTATTTAGC CAGTATGTTC TCTAGTGTGG TTCGTTGTTT TTGCGTGAGC
      301 CATGAGAACG AACCATTGAG ATCATGCTTA CTTTGCATGT CACTCAAAAA TTTTGCCTCA
      361 AAACTGGTGA GCTGAATTTT TGCAGTTAAA GCATCGTGTA GTGTTTTTCT TAGTCCGTTA
      421 CGTAGGTAGG AATCTGATGT AATGGTTGTT GGTATTTTGT CACCATTCAT TTTTATCTGG
      481 TTGTTCTCAA GTTCGGTTAC GAGATCCATT TGTCTATCTA GTTCAACTTG GAAAATCAAC
      541 GTATCAGTCG GGCGGCCTCG CTTATCAACC ACCAATTTCA TATTGCTGTA AGTGTTTAAA
      601 TCTTTACTTA TTGGTTTCAA AACCCATTGG TTAAGCCTTT TAAACTCATG GTAGTTATTT
      661 TCAAGCATTA ACATGAACTT AAATTCATCA AGGCTAATCT CTATATTTGC CTTGTGAGTT
      721 TTCTTTTGTG TTAGTTCTTT TAATAACCAC TCATAAATCC TCATAGAGTA TTTGTTTTCA
      781 AAAGACTTAA CATGTTCCAG ATTATATTTT ATGAATTTTT TTAACTGGAA AAGATAAGGC
      841 AATATCTCTT CACTAAAAAC TAATTCTAAT TTTTCGCTTG AGAACTTGGC ATAGTTTGTC
      901 CACTGGAAAA TCTCAAAGCC TTTAACCAAA GGATTCCTGA TTTCCACAGT TCTCGTCATC
      961 AGCTCTCTGG TTGCTTTAGC TAATACACCA TAAGCATTTT CCCTACTGAT GTTCATCATC
     1021 TGAGCGTATT GGTTATAAGT GAACGATACC GTCCGTTCTT TCCTTGTAGG GTTTTCAATC
     1081 GTGGGGTTGA GTAGTGCCAC ACAGCATAAA ATTAGCTTGG TTTCATGCTC CGTTAAGTCA
     1141 TAGCGACTAA TCGCTAGTTC ATTTGCTTTG AAAACAACTA ATTCAGACAT ACATCTCAAT
     1201 TGGTCTAggt gattttaatc actataccaa ttgagatggg ctagtcaatg ataattacat
     1261 gtccTTTTCC TTTGAGTTGT GGGTATCTGT AAATTCTGCT AGACCTTTGC TGGAAAACTT
     1321 GTAAATTCTG CTAGACCCTC TGTAAATTCC GCTAGACCTT TGTGTGTTTT TTTTGTTTAT
     1381 ATTCAAGTGG TTATAATTTA TAGAATAAAG AAAGAATAAA AAAAGATAAA AAGAATAGAT
     1441 CCCAGCCCTG TGTATAACTC ACTACTTTAG TCAGTTCCGC AGTATTACAA AAGGATGTCG
     1501 CAAACGCTGT TTGCTCCTCT ACAAAACAGA CCTTAAAACC CTAAAGGCTT AAGTAGCACC
     1561 CTCGCAAGCT CGGGCAAATC GCTGAATATT CCTTTTGTCT CCGACCATCA GGCACCTGAG
     1621 TCGCTGTCTT TTTCGTGACA TTCAGTTCGC TGCGCTCACG GCTCTGGCAG TGAATGGGGG
     1681 TAAATGGCAC TACAGGCGCC TTTTATGGAT TCATGCAAGG AAACTACCCA TAATACAAGA
     1741 AAAGCCCGTC ACGGGCTTCT CAGGGCGTTT TATGGCGGGT CTGCTATGTG GTGCTATCTG
     1801 ACTTTTTGCT GTTCAGCAGT TCCTGCCCTC TGATTTTCCA GTCTGACCAC TTCGGATTAT
     1861 CCCGTGACAG GTCATTCAGA CTGGCTAATG CACCCAGTAA GGCAGCGGTA TCATCAACAG
     1921 GCTTACCCGT CTTACTGTCC CTAGTGCTTG GATTCTCACC AATAAAAAAC GCCCGGCGGC
     1981 AACCGAGCGT TCTGAACAAA TCCAGATGGA GTTCTGAGGT CATTACTGGA TCTATCAACA
     2041 GGAGTCCAAG CGAGCTCGTA AACTTGGTCT GACAGTTACC AATGCTTAAT CAGTGAGGCA
     2101 CCTATCTCAG CGATCTGTCT ATTTCGTTCA TCCATAGTTG CCTGACTCCC CGTCGTGTAG
     2161 ATAACTACGA TACGGGAGGG CTTACCATCT GGCCCCAGTG CTGCAATGAT ACCGCGTGAT
     2221 CCACGCTCAC CGGCTCCAGA TTTATCAGCA ATAAACCAGC CAGCCGGAAG GGCCGAGCGC
     2281 AGAAGTGGTC CTGCAACTTT ATCCGCCTCC ATCCAGTCTA TTAATTGTTG CCGGGAAGCT
     2341 AGAGTAAGTA GTTCGCCAGT TAATAGTTTG CGCAACGTTG TTGCCATTGC TACAGGCATC
     2401 GTGGTGTCAC GCTCGTCGTT TGGTATGGCT TCATTCAGCT CCGGTTCCCA ACGATCAAGG
     2461 CGAGTTACAT GATCCCCCAT GTTGTGCAAA AAAGCGGTTA GCTCCTTCGG TCCTCCGATC
     2521 GTTGTCAGAA GTAAGTTGGC CGCAGTGTTA TCACTCATGG TTATGGCAGC ACTGCATAAT
     2581 TCTCTTACTG TCATGCCATC CGTAAGATGC TTTTCTGTGA CTGGTGAGTA CTCAACCAAG
     2641 TCATTCTGAG AATAGTGTAT GCGGCGACCG AGTTGCTCTT GCCCGGCGTC AATACGGGAT
     2701 AATACCGCGC CACATAGCAG AACTTTAAAA GTGCTCATCA TTGGAAAACG TTCTTCGGGG
     2761 CGAAAACTCT CAAGGATCTT ACCGCTGTTG AGATCCAGTT CGATGTAACC CACTCGTGCA
     2821 CCCAACTGAT CTTCAGCATC TTTTACTTTC ACCAGCGTTT CTGGGTGAGC AAAAACAGGA
     2881 AGGCAAAATG CCGCAAAAAA GGGAATAAGG GCGACACGGA AATGTTGAAT ACTCATACTC
     2941 TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA
     3001 TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CATGGTGCCA
     3061 CCTGACGTCT AAGAAACCAT TATTATCATG ACATTAACCT ATAAAAATAG GCGTATCACG
     3121 AGGCAGAATT Tttatcgctt ccgcgttccg tctactccct attacgctcc ggaatctccg
     3181 acgcctacgg ccgacgtcgg accgtgtcgt ccacggatat caatgaccta tcttttcttg
     3241 cttccgactt cgtttaagat tggttaatta acttctgatt atgattttca cattctttcg
     3301 gcagaagata tgaccttaac tgcataaatg ccatataatt tagctatagg gatgctgaaa
     3361 tagcgtttcg tgaagaacag gagatgggta aAAAGAGGAG AAATACTAGA TGGTTTCGAA
     3421 AGGTGAGGAG GATAACATGG CGTCGCTGCC TGCTACACAT GAACTGCATA TTTTCGGCAG
     3481 CATCAACGGA GTTGATTTCG ACATGGTGGG CCAAGGCACC GGCAACCCGA ACGACGGCTA
     3541 TGAAGAATTG AATTTGAAAT CTACCAAGGG CGACTTGCAA TTTAGTCCGT GGATTCTGGT
     3601 ACCGCATATT GGTTATGGTT TTCATCAGTA TCTGCCCTAC CCAGACGGAA TGAGCCCATT
     3661 TCAGGCGGCT ATGGTGGATG GCAGTGGCTA TCAGGTCCAT CGCACCATGC AATTCGAGGA
     3721 TGGGGCGAGC CTGACCGTGA ATTATCGGTA CACTTACGAA GGTTCACATA TTAAAGGTGA
     3781 AGCACAAGTT AAAGGCACCG GCTTCCCGGC GGATGGCCCA GTGATGACCA ACAGCCTGAC
     3841 GGCGGCGGAC TGGTGTCGTA GCAAAAAGAC GTATCCCAAT GATAAAACAA TTATTTCTAC
     3901 CTTTAAATGG AGTTACACCA CGGGTAACGG TAAACGCTAC CGTAGCACGG CTCGCACCAC
     3961 ATACACCTTT GCGAAACCCA TGGCCGCGAA CTATCTGAAA AATCAGCCGA TGTATGTGTT
     4021 TCGCAAAACG GAACTGAAAC ATTCGAAAAC TGAACTCAAT TTCAAAGAGT GGCAAAAAGC
     4081 CTTCACCGAC GTAATGGGCA TGGATGAACT GTACAAATGA CCAGGCATCA AATAAAACGA
     4141 AAGGCTCAGT CGAAAGACTG GGCCTTTCGT TTTATCTGTT GTTTGTCGGT GAACGCTCTC
     4201 TACTAGAGTC ACACTGGCTC ACCTTCGGGT GGGCCTTTCT GCGTTTATAG CAAGGTGTCA
     4261 CCACCCTGCC CTTTTTCTTT AAAACCGAAA AGATTACTTC GCGTTATGCA GGCTTCCTCG
     4321 CTCACTGACT CGCTGCGCTC GGTCGTTCGG CTGCGG
//