LOCUS       Exported                9492 bp ds-DNA     circular SYN 28-OCT-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9492)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 28, 2018 from SnapGene Viewer 4.2.6
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9492
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    2136..2154
                     /label=M13 reverse priming site
                     /note="M13 reverse priming site"
     enhancer        2420..2797
                     /label=hCMV
                     /note="hCMV"
     promoter        2803..4144
                     /label=AG hybrid promoter
                     /note="AG hybrid promoter"
     intron          3172..4096
                     /note="intron"
     misc_feature    4145..4251
                     /label=Untitled
     gene            complement(4151..4184)
                     /label=loxP
                     /note="loxP"
     gene            4186..4219
                     /label=FRT
                     /note="FRT"
     gene            4220..4285
                     /label=EM7 promoter
                     /note="EM7 promoter"
     misc_feature    4220..4251
                     /label=Untitled
     CDS             4286..5089
                     /label=Neo
                     /note="Neo"
     misc_feature    5077..5136
                     /label=Untitled
     misc_feature    5077..5101
                     /label=Untitled
     gene            5102..5135
                     /label=FRT
                     /note="FRT"
     misc_feature    5136
                     /label=Untitled
     misc_feature    5136
                     /label=Untitled
     gene            5142..5440
                     /label=bGHpA
                     /note="bGHpA"
     misc_feature    5465..5692
                     /label=SV40 pA
                     /note="SV40 pA"
     misc_feature    5698..5925
                     /label=SV40 pA
                     /note="SV40 pA"
     misc_feature    5931..6158
                     /label=SV40 pA
                     /note="SV40 pA"
     gene            complement(6181..6214)
                     /label=loxP
                     /note="loxP"
     misc_feature    6224..7684
                     /label=tdGFP
                     /note="tdGFP"
     misc_feature    6224..6937
                     /label=superfolder GFP
                     /note="superfolder GFP"
     misc_feature    6938..6967
                     /label=linker
                     /note="linker"
     misc_feature    7693..8278
                     /label=WPRE
     misc_feature    8299..8600
                     /label=bGH pA
                     /note="bGH pA"
     misc_feature    complement(8847..8868)
                     /label=T7 priming site
                     /note="T7 priming site"
     misc_feature    complement(8877..8893)
                     /label=M13 F2 priming site
                     /note="M13 F2 priming site"
ORIGIN
        1 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
       61 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
      121 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
      181 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
      241 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
      301 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
      361 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
      421 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
      481 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
      541 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
      601 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
      661 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
      721 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
      781 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
      841 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
      901 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
      961 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     1021 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     1081 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     1141 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     1201 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     1261 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc
     1321 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     1381 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     1441 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     1501 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     1561 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     1621 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     1681 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     1741 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     1801 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     1861 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     1921 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     1981 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
     2041 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
     2101 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg
     2161 ccaagcgcgc aattaagttt aaaccggcgg atcacaagca ataataacct gtagttttgc
     2221 tgcataaaac cccagatgac tacctatcct cccattttcc ttatttgccc ctattaaaaa
     2281 acttcccgac aaaaccgaaa atctgtggga agtcttgtcc ctccaatttt acacctgttc
     2341 aattcccctg caggacaacg cccacacacc aggttagcct ttaagcctgc ccagaagact
     2401 cccgcccatc ttctaggtcg acattgatta ttgactagtt attaatagta atcaattacg
     2461 gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc
     2521 ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc
     2581 atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact
     2641 gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat
     2701 gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact
     2761 tggcagtaca tctacgtatt agtcatcgct attaccatgg tcgaggtgag ccccacgttc
     2821 tgcttcactc tccccatctc ccccccctcc ccacccccaa ttttgtattt atttattttt
     2881 taattatttt gtgcagcgat gggggcgggg gggggggggg ggcccccccc aggcggggcg
     2941 gggcggggcg aggggcgggg cggggcgagg cggaaaggtg cggcggcagc caatcagagc
     3001 ggcgcgctcc gaaagtttcc ttttatggcg aggcggcggc ggcggcggcc ctataaaaag
     3061 cgaagcgcgc ggcgggcggg agtcgttgcg cgctgccttc cccccgtgcc ccgctccgcc
     3121 gccgcctcgc gccgcccgcc ccggctctga ctgaccgcgt tactcccaca ggtgagcggg
     3181 cgggacggcc cttctcctcc gggctgtaat tagcgcttgg tttaatgacg gcttgtttct
     3241 tttctgtggc tgcgtgaaag ccttgagggg ctccgggagg gccctttgtg cggggggagc
     3301 ggctcggggg gtgcgtgcgt gtgtgtgtgc gtggggagcg ccgcgtgcgg ctccgcgctg
     3361 cccggcggct gtgagcgctg cgggcgcggc gcggggcttt gtgcgctccg cagtgtgcgc
     3421 gaggggagcg cggccggggg cggtgccccg cggtgcgggg ggggctgcga ggggaacaaa
     3481 ggctgcgtgc ggggtgtgtg cgtggggggg tgagcagggg gtgtgggcgc gtcggtcggg
     3541 ctgcaacccc ccctgcaccc ccctccccga gttgctgagc acggcccggc ttcgggtgcg
     3601 gggctccgta cggggcgtgg cgcggggctc gccgtgccgg gcggggggtg gcggcaggtg
     3661 ggggtgccgg gcggggcggg gccgcctcgg gccggggagg gctcggggga aggggcgcgg
     3721 cggcccccgg agcgccggcg gctgtcgagg cgcggcgagc cgcagccatt gccttttatg
     3781 gtaatcgtgc gagagggcgc agggacttcc tttgtcccaa atctgtgcgg agccgaaatc
     3841 tgggaggcgc cgccgcaccc cctctagcgg gcgcggggcg aagcggtgcg gcgccggcag
     3901 gaaggaaatg ggcggggagg gccttcgtgc gtcgccgcgc cgccgtcccc ttctccctct
     3961 ccagcctcgg ggctgtccgc ggggggacgg ctgccttcgg gggggacggg gcagggcggg
     4021 gttcggcttc tggcgtgtga ccggcggctc tagagcctct gctaaccatg ttcatgcctt
     4081 cttctttttc ctacagctcc tgggcaacgt gctggttatt gtgctgtctc atcattttgg
     4141 caaagaattc ataacttcgt atagcataca ttatacgaag ttatagaagt tcctattctc
     4201 tagaaagtat aggaacttcg ttgacaatta atcatcggca tagtatatcg gcatagtata
     4261 atacgacaag gtgaggaact aaaccatggg atcggccatt gaacaagatg gattgcacgc
     4321 aggttctccg gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat
     4381 cggctgctct gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt
     4441 caagaccgac ctgtccggtg ccctgaatga actgcaggac gaggcagcgc ggctatcgtg
     4501 gctggccacg acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag
     4561 ggactggctg ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc
     4621 tgccgagaaa gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc
     4681 tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga
     4741 agccggtctt gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga
     4801 actgttcgcc aggctcaagg cgcgcatgcc cgacggcgat gatctcgtcg tgacccatgg
     4861 cgatgcctgc ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg
     4921 tggccggctg ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc
     4981 tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc
     5041 cgattcgcag cgcatcgcct tctatcgcct tcttgacgag ttcttctgag gggatcaatt
     5101 cgaagttcct attctctaga aagtatagga acttcgctag cagctcgctg atcagcctcg
     5161 actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc
     5221 ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt
     5281 ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat
     5341 tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa
     5401 agaaccagct ggggctcgac tagagcttgc ggaaccctta ccaccgcggt ggcggccgct
     5461 ctagaatcat aatcagcctt accacatttg tagaggtttt acttgcttta aaaaacctcc
     5521 cacacctccc cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt aacttgttta
     5581 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
     5641 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct taactagaat
     5701 cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct
     5761 ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc
     5821 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
     5881 actgcattct agttgtggtt tgtccaaact catcaatgta tcttaactag aatcataatc
     5941 agccatacca catttgtaga ggttttactt gctttaaaaa acctcccaca cctccccctg
     6001 aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc agcttataat
     6061 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
     6121 tctagttgtg gtttgtccaa actcatcaat gtatcttaac tagtggatct catgcctagg
     6181 ataacttcgt atagcataca ttatacgaag ttataggcgc gccatgagca aaggagaaga
     6241 acttttcact ggagttgtcc caattcttgt tgaattagat ggtgatgtta atgggcacaa
     6301 attttctgtc cgtggagagg gtgaaggtga tgctacaaac ggaaaactca cccttaaatt
     6361 tatttgcact actggaaaac tacctgttcc gtggccaaca cttgtcacta ctctgaccta
     6421 tggtgttcaa tgcttttccc gttatccgga tcacatgaaa cggcatgact ttttcaagag
     6481 tgccatgccc gaaggttatg tacaggaacg cactatatct ttcaaagatg acgggaccta
     6541 caagacgcgt gctgaagtca agtttgaagg tgataccctt gttaatcgta tcgagttaaa
     6601 gggtattgat tttaaagaag atggaaacat tcttggacac aaactcgagt acaactttaa
     6661 ctcacacaat gtatacatca cggcagacaa acaaaagaat ggaatcaaag ctaacttcaa
     6721 aattcgccac aacgttgaag atggttccgt tcaactagca gaccattatc aacaaaatac
     6781 tccaattggc gatggccctg tccttttacc agacaaccat tacctgtcga cacaatctgt
     6841 cctttcgaaa gatcccaacg aaaagcgtga ccacatggtc cttcttgagt ttgtaactgc
     6901 tgctgggatt acacatggca tggatgagct ctacaaaggt tctgctggat ctgcagcagg
     6961 atctggtatg agcaaaggag aagaactttt cactggagtt gtcccaattc ttgttgaatt
     7021 agatggtgat gttaatgggc acaaattttc tgtccgtgga gagggtgaag gtgatgctac
     7081 aaacggaaaa ctcaccctta aatttatttg cactactgga aaactacctg ttccgtggcc
     7141 aacacttgtc actactctga cctatggtgt tcaatgcttt tcccgttatc cggatcacat
     7201 gaaacggcat gactttttca agagtgccat gcccgaaggt tatgtacagg aacgcactat
     7261 atctttcaaa gatgacggga cctacaagac gcgtgctgaa gtcaagtttg aaggtgatac
     7321 ccttgttaat cgtatcgagt taaagggtat tgattttaaa gaagatggaa acattcttgg
     7381 acacaaactc gagtacaact ttaactcaca caatgtatac atcacggcag acaaacaaaa
     7441 gaatggaatc aaagctaact tcaaaattcg ccacaacgtt gaagatggtt ccgttcaact
     7501 agcagaccat tatcaacaaa atactccaat tggcgatggc cctgtccttt taccagacaa
     7561 ccattacctg tcgacacaat ctgtcctttc gaaagatccc aacgaaaagc gtgaccacat
     7621 ggtccttctt gagtttgtaa ctgctgctgg gattacacat ggcatggatg agctctacaa
     7681 atgattaatt aaaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact
     7741 atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg
     7801 cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg
     7861 aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa
     7921 cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc
     7981 ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg
     8041 ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt
     8101 ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt
     8161 cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc
     8221 cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcagg
     8281 atccactaga gcggccgcct agagctcgct gatcagcctc gactgtgcct tctagttgcc
     8341 agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca
     8401 ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta
     8461 ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc
     8521 atgctgggga tgcggtgggc tctatggctt ctgaggcgga aagaaccagc tggggctcga
     8581 ctagagcttg cggaaccctt aagcttaaag actggagttg cagatcacga gggaagaggg
     8641 ggaagggatt ctcccaggcc cagggcggtc ctcagaagcc aggaggcagc agagaactcc
     8701 cagaaaggta ttgcaacact cccctccccc ctccggagaa gggtgcggcc ttctccccgc
     8761 ctactccact gcagctccct tactgataac aactcagagc gactttggga gagcaagtgc
     8821 tcgagggggg gcccggtacc caattcgccc tatagtgagt cgtattacgc gcgctcactg
     8881 gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt
     8941 gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct
     9001 tcccaacagt tgcgcagcct gaatggcgaa tggaaattgt aagcgttaat attttgttaa
     9061 aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca
     9121 aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga
     9181 acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc
     9241 agggcgatgg cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc
     9301 gtaaagcact aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc
     9361 cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg
     9421 caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac
     9481 agggcgcgtc ag
//
