LOCUS       Exported                9487 bp ds-DNA     circular SYN 28-OCT-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9487)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 28, 2018 from SnapGene Viewer 4.2.6
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9487
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    2136..2154
                     /label=M13 reverse priming site
                     /note="M13 reverse priming site"
     enhancer        2420..2797
                     /label=hCMV
                     /note="hCMV"
     promoter        2803..4144
                     /label=AG hybrid promoter
                     /note="AG hybrid promoter"
     intron          3172..4096
                     /note="intron"
     misc_feature    4146..4249
                     /label=Untitled
     misc_feature    complement(4151..4182)
                     /label=rox
                     /note="rox"
     misc_feature    complement(4151..4182)
                     /label=Untitled
     gene            4184..4217
                     /label=FRT
                     /note="FRT"
     gene            4218..4283
                     /label=EM7 promoter
                     /note="EM7 promoter"
     misc_feature    4218..4249
                     /label=Untitled
     CDS             4284..5087
                     /label=Neo
                     /note="Neo"
     misc_feature    5075..5134
                     /label=Untitled
     misc_feature    5075..5099
                     /label=Untitled
     gene            5100..5133
                     /label=FRT
                     /note="FRT"
     misc_feature    5134
                     /label=Untitled
     misc_feature    5134
                     /label=Untitled
     gene            5140..5438
                     /label=bGHpA
                     /note="bGHpA"
     misc_feature    5453..5462
                     /label=Xba1-pA-for
                     /note="Xba1-pA-for"
     misc_feature    5463..5690
                     /label=SV40 pA
                     /note="SV40 pA"
     misc_feature    5463..5489
                     /label=3xstop-for
                     /note="3xstop-for"
     misc_feature    complement(5476..5478)
                     /label=probably Taq error
                     /note="probably Taq error"
     misc_feature    complement(5670..5690)
                     /label=Untitled
     misc_feature    5696..5923
                     /label=SV40 pA
                     /note="SV40 pA"
     misc_feature    5696..5716
                     /label=Untitled
     misc_feature    complement(5903..5923)
                     /label=Untitled
     misc_feature    5929..6156
                     /label=SV40 pA
                     /note="SV40 pA"
     misc_feature    5929..5949
                     /label=Untitled
     misc_feature    complement(6041..6046)
                     /label=Untitled
     misc_feature    6061..6066
                     /label=Untitled
     misc_feature    6090..6095
                     /label=Untitled
     misc_feature    complement(6130..6156)
                     /label=3xstop-rev
                     /note="3xstop-rev"
     misc_feature    complement(6136..6156)
                     /label=pA-Spe1-rev-RC
                     /note="pA-Spe1-rev-RC"
     misc_feature    complement(6179..6210)
                     /label=rox
                     /note="rox"
     misc_feature    complement(6179..6210)
                     /label=Untitled
     misc_feature    6219..7679
                     /label=tdGFP
                     /note="tdGFP"
     misc_feature    6219..6932
                     /label=superfolder GFP
                     /note="superfolder GFP"
     misc_feature    6933..6962
                     /label=linker
                     /note="linker"
     misc_feature    7688..8273
                     /label=WPRE
     misc_feature    7688..7723
                     /label=Untitled
     misc_feature    complement(8254..8274)
                     /label=Untitled
     misc_feature    complement(8254..8273)
                     /label=Untitled
     misc_feature    8294..8595
                     /label=bGH pA
                     /note="bGH pA"
     misc_feature    complement(8842..8863)
                     /label=T7 priming site
                     /note="T7 priming site"
     misc_feature    complement(8872..8888)
                     /label=M13 F2 priming site
                     /note="M13 F2 priming site"
ORIGIN
        1 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
       61 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
      121 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
      181 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
      241 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
      301 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
      361 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
      421 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
      481 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
      541 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
      601 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
      661 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
      721 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
      781 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
      841 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
      901 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
      961 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     1021 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     1081 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     1141 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     1201 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     1261 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc
     1321 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     1381 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     1441 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     1501 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     1561 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     1621 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     1681 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     1741 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     1801 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     1861 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     1921 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     1981 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
     2041 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
     2101 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg
     2161 ccaagcgcgc aattaagttt aaaccggcgg atcacaagca ataataacct gtagttttgc
     2221 tgcataaaac cccagatgac tacctatcct cccattttcc ttatttgccc ctattaaaaa
     2281 acttcccgac aaaaccgaaa atctgtggga agtcttgtcc ctccaatttt acacctgttc
     2341 aattcccctg caggacaacg cccacacacc aggttagcct ttaagcctgc ccagaagact
     2401 cccgcccatc ttctaggtcg acattgatta ttgactagtt attaatagta atcaattacg
     2461 gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc
     2521 ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc
     2581 atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact
     2641 gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat
     2701 gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact
     2761 tggcagtaca tctacgtatt agtcatcgct attaccatgg tcgaggtgag ccccacgttc
     2821 tgcttcactc tccccatctc ccccccctcc ccacccccaa ttttgtattt atttattttt
     2881 taattatttt gtgcagcgat gggggcgggg gggggggggg ggcccccccc aggcggggcg
     2941 gggcggggcg aggggcgggg cggggcgagg cggaaaggtg cggcggcagc caatcagagc
     3001 ggcgcgctcc gaaagtttcc ttttatggcg aggcggcggc ggcggcggcc ctataaaaag
     3061 cgaagcgcgc ggcgggcggg agtcgttgcg cgctgccttc cccccgtgcc ccgctccgcc
     3121 gccgcctcgc gccgcccgcc ccggctctga ctgaccgcgt tactcccaca ggtgagcggg
     3181 cgggacggcc cttctcctcc gggctgtaat tagcgcttgg tttaatgacg gcttgtttct
     3241 tttctgtggc tgcgtgaaag ccttgagggg ctccgggagg gccctttgtg cggggggagc
     3301 ggctcggggg gtgcgtgcgt gtgtgtgtgc gtggggagcg ccgcgtgcgg ctccgcgctg
     3361 cccggcggct gtgagcgctg cgggcgcggc gcggggcttt gtgcgctccg cagtgtgcgc
     3421 gaggggagcg cggccggggg cggtgccccg cggtgcgggg ggggctgcga ggggaacaaa
     3481 ggctgcgtgc ggggtgtgtg cgtggggggg tgagcagggg gtgtgggcgc gtcggtcggg
     3541 ctgcaacccc ccctgcaccc ccctccccga gttgctgagc acggcccggc ttcgggtgcg
     3601 gggctccgta cggggcgtgg cgcggggctc gccgtgccgg gcggggggtg gcggcaggtg
     3661 ggggtgccgg gcggggcggg gccgcctcgg gccggggagg gctcggggga aggggcgcgg
     3721 cggcccccgg agcgccggcg gctgtcgagg cgcggcgagc cgcagccatt gccttttatg
     3781 gtaatcgtgc gagagggcgc agggacttcc tttgtcccaa atctgtgcgg agccgaaatc
     3841 tgggaggcgc cgccgcaccc cctctagcgg gcgcggggcg aagcggtgcg gcgccggcag
     3901 gaaggaaatg ggcggggagg gccttcgtgc gtcgccgcgc cgccgtcccc ttctccctct
     3961 ccagcctcgg ggctgtccgc ggggggacgg ctgccttcgg gggggacggg gcagggcggg
     4021 gttcggcttc tggcgtgtga ccggcggctc tagagcctct gctaaccatg ttcatgcctt
     4081 cttctttttc ctacagctcc tgggcaacgt gctggttatt gtgctgtctc atcattttgg
     4141 caaagaattc taactttaaa taattggcat tatttaaagt taagaagttc ctattctcta
     4201 gaaagtatag gaacttcgtt gacaattaat catcggcata gtatatcggc atagtataat
     4261 acgacaaggt gaggaactaa accatgggat cggccattga acaagatgga ttgcacgcag
     4321 gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg
     4381 gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca
     4441 agaccgacct gtccggtgcc ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc
     4501 tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg
     4561 actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg
     4621 ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta
     4681 cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag
     4741 ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac
     4801 tgttcgccag gctcaaggcg cgcatgcccg acggcgatga tctcgtcgtg acccatggcg
     4861 atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg
     4921 gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg
     4981 aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg
     5041 attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgaggg gatcaattcg
     5101 aagttcctat tctctagaaa gtataggaac ttcgctagca gctcgctgat cagcctcgac
     5161 tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct
     5221 ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct
     5281 gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg
     5341 ggaagacaat agcaggcatg ctggggatgc ggtgggctct atggcttctg aggcggaaag
     5401 aaccagctgg ggctcgacta gagcttgcgg aacccttacc accgcggtgg cggccgctct
     5461 agaatcataa tcagccttac cacatttgta gaggttttac ttgctttaaa aaacctccca
     5521 cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa cttgtttatt
     5581 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
     5641 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta actagaatca
     5701 taatcagcca taccacattt gtagaggttt tacttgcttt aaaaaacctc ccacacctcc
     5761 ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt taacttgttt attgcagctt
     5821 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     5881 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttaactagaa tcataatcag
     5941 ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa
     6001 cctgaaacat aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg
     6061 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
     6121 tagttgtggt ttgtccaaac tcatcaatgt atcttaacta gtggatctca tgcctaggta
     6181 actttaaata attggcatta tttaaagtta ggcgcgccat gagcaaagga gaagaacttt
     6241 tcactggagt tgtcccaatt cttgttgaat tagatggtga tgttaatggg cacaaatttt
     6301 ctgtccgtgg agagggtgaa ggtgatgcta caaacggaaa actcaccctt aaatttattt
     6361 gcactactgg aaaactacct gttccgtggc caacacttgt cactactctg acctatggtg
     6421 ttcaatgctt ttcccgttat ccggatcaca tgaaacggca tgactttttc aagagtgcca
     6481 tgcccgaagg ttatgtacag gaacgcacta tatctttcaa agatgacggg acctacaaga
     6541 cgcgtgctga agtcaagttt gaaggtgata cccttgttaa tcgtatcgag ttaaagggta
     6601 ttgattttaa agaagatgga aacattcttg gacacaaact cgagtacaac tttaactcac
     6661 acaatgtata catcacggca gacaaacaaa agaatggaat caaagctaac ttcaaaattc
     6721 gccacaacgt tgaagatggt tccgttcaac tagcagacca ttatcaacaa aatactccaa
     6781 ttggcgatgg ccctgtcctt ttaccagaca accattacct gtcgacacaa tctgtccttt
     6841 cgaaagatcc caacgaaaag cgtgaccaca tggtccttct tgagtttgta actgctgctg
     6901 ggattacaca tggcatggat gagctctaca aaggttctgc tggatctgca gcaggatctg
     6961 gtatgagcaa aggagaagaa cttttcactg gagttgtccc aattcttgtt gaattagatg
     7021 gtgatgttaa tgggcacaaa ttttctgtcc gtggagaggg tgaaggtgat gctacaaacg
     7081 gaaaactcac ccttaaattt atttgcacta ctggaaaact acctgttccg tggccaacac
     7141 ttgtcactac tctgacctat ggtgttcaat gcttttcccg ttatccggat cacatgaaac
     7201 ggcatgactt tttcaagagt gccatgcccg aaggttatgt acaggaacgc actatatctt
     7261 tcaaagatga cgggacctac aagacgcgtg ctgaagtcaa gtttgaaggt gatacccttg
     7321 ttaatcgtat cgagttaaag ggtattgatt ttaaagaaga tggaaacatt cttggacaca
     7381 aactcgagta caactttaac tcacacaatg tatacatcac ggcagacaaa caaaagaatg
     7441 gaatcaaagc taacttcaaa attcgccaca acgttgaaga tggttccgtt caactagcag
     7501 accattatca acaaaatact ccaattggcg atggccctgt ccttttacca gacaaccatt
     7561 acctgtcgac acaatctgtc ctttcgaaag atcccaacga aaagcgtgac cacatggtcc
     7621 ttcttgagtt tgtaactgct gctgggatta cacatggcat ggatgagctc tacaaatgat
     7681 taattaaaac ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt
     7741 gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc
     7801 cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag
     7861 ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc
     7921 actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc
     7981 cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg
     8041 ctgttgggca ctgacaattc cgtggtgttg tcggggaaat catcgtcctt tccttggctg
     8101 ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc
     8161 ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt
     8221 cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc gcaggatcca
     8281 ctagagcggc cgcctagagc tcgctgatca gcctcgactg tgccttctag ttgccagcca
     8341 tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc
     8401 ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg
     8461 gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct
     8521 ggggatgcgg tgggctctat ggcttctgag gcggaaagaa ccagctgggg ctcgactaga
     8581 gcttgcggaa cccttaagct taaagactgg agttgcagat cacgagggaa gagggggaag
     8641 ggattctccc aggcccaggg cggtcctcag aagccaggag gcagcagaga actcccagaa
     8701 aggtattgca acactcccct cccccctccg gagaagggtg cggccttctc cccgcctact
     8761 ccactgcagc tcccttactg ataacaactc agagcgactt tgggagagca agtgctcgag
     8821 ggggggcccg gtacccaatt cgccctatag tgagtcgtat tacgcgcgct cactggccgt
     8881 cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc
     8941 acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca
     9001 acagttgcgc agcctgaatg gcgaatggaa attgtaagcg ttaatatttt gttaaaattc
     9061 gcgttaaatt tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaaaatc
     9121 ccttataaat caaaagaata gaccgagata gggttgagtg ttgttccagt ttggaacaag
     9181 agtccactat taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc
     9241 gatggcccac tacgtgaacc atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa
     9301 gcactaaatc ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg
     9361 aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt
     9421 gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc
     9481 gcgtcag
//
