LOCUS       Exported                9457 bp ds-DNA     circular SYN 28-OCT-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9457)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 28, 2018 from SnapGene Viewer 4.2.6
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9457
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    2136..2154
                     /label=M13 reverse priming site
                     /note="M13 reverse priming site"
     enhancer        2420..2797
                     /label=hCMV
                     /note="hCMV"
     promoter        2803..4144
                     /label=AG hybrid promoter
                     /note="AG hybrid promoter"
     intron          3172..4096
                     /note="intron"
     misc_feature    4146..4249
                     /label=Untitled
     misc_feature    complement(4151..4182)
                     /label=rox
                     /note="rox"
     misc_feature    complement(4151..4182)
                     /label=Untitled
     gene            4184..4217
                     /label=FRT
                     /note="FRT"
     gene            4218..4283
                     /label=EM7 promoter
                     /note="EM7 promoter"
     misc_feature    4218..4249
                     /label=Untitled
     CDS             4284..5087
                     /label=Neo
                     /note="Neo"
     misc_feature    5075..5134
                     /label=Untitled
     misc_feature    5075..5099
                     /label=Untitled
     gene            5100..5133
                     /label=FRT
                     /note="FRT"
     misc_feature    5134
                     /label=Untitled
     misc_feature    5134
                     /label=Untitled
     gene            5140..5438
                     /label=bGHpA
                     /note="bGHpA"
     misc_feature    5453..5462
                     /label=Xba1-pA-for
                     /note="Xba1-pA-for"
     misc_feature    5463..5690
                     /label=SV40 pA
                     /note="SV40 pA"
     misc_feature    5463..5489
                     /label=3xstop-for
                     /note="3xstop-for"
     misc_feature    complement(5476..5478)
                     /label=probably Taq error
                     /note="probably Taq error"
     misc_feature    complement(5670..5690)
                     /label=Untitled
     misc_feature    5696..5923
                     /label=SV40 pA
                     /note="SV40 pA"
     misc_feature    5696..5716
                     /label=Untitled
     misc_feature    complement(5903..5923)
                     /label=Untitled
     misc_feature    5929..6156
                     /label=SV40 pA
                     /note="SV40 pA"
     misc_feature    5929..5949
                     /label=Untitled
     misc_feature    complement(6041..6046)
                     /label=Untitled
     misc_feature    6061..6066
                     /label=Untitled
     misc_feature    6090..6095
                     /label=Untitled
     misc_feature    complement(6130..6156)
                     /label=3xstop-rev
                     /note="3xstop-rev"
     misc_feature    complement(6136..6156)
                     /label=pA-Spe1-rev-RC
                     /note="pA-Spe1-rev-RC"
     misc_feature    complement(6179..6210)
                     /label=rox
                     /note="rox"
     misc_feature    complement(6179..6210)
                     /label=Untitled
     misc_feature    6219..7649
                     /label=tdTomato
                     /note="tdTomato"
     misc_feature    6219..6239
                     /label=Untitled
     misc_feature    6219..6239
                     /label=HN921
                     /note="HN921"
     misc_feature    6882..6965
                     /label=linker
                     /note="linker"
     misc_feature    complement(6886..6903)
                     /label=Untitled
     misc_feature    6911
                     /label=c to t Age1
                     /note="c to t Age1"
     misc_feature    complement(7628..7649)
                     /label=HN304
                     /note="HN304"
     misc_feature    7658..8243
                     /label=WPRE
     misc_feature    7658..7693
                     /label=Untitled
     misc_feature    complement(8224..8244)
                     /label=Untitled
     misc_feature    complement(8224..8243)
                     /label=Untitled
     misc_feature    8264..8565
                     /label=bGH pA
                     /note="bGH pA"
     misc_feature    complement(8812..8833)
                     /label=T7 priming site
                     /note="T7 priming site"
     misc_feature    complement(8842..8858)
                     /label=M13 F2 priming site
                     /note="M13 F2 priming site"
ORIGIN
        1 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
       61 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
      121 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
      181 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
      241 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
      301 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
      361 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
      421 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
      481 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
      541 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
      601 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
      661 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
      721 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
      781 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
      841 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
      901 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
      961 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     1021 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     1081 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     1141 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     1201 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     1261 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc
     1321 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     1381 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     1441 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     1501 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     1561 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     1621 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     1681 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     1741 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     1801 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     1861 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     1921 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     1981 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
     2041 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
     2101 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg
     2161 ccaagcgcgc aattaagttt aaaccggcgg atcacaagca ataataacct gtagttttgc
     2221 tgcataaaac cccagatgac tacctatcct cccattttcc ttatttgccc ctattaaaaa
     2281 acttcccgac aaaaccgaaa atctgtggga agtcttgtcc ctccaatttt acacctgttc
     2341 aattcccctg caggacaacg cccacacacc aggttagcct ttaagcctgc ccagaagact
     2401 cccgcccatc ttctaggtcg acattgatta ttgactagtt attaatagta atcaattacg
     2461 gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc
     2521 ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc
     2581 atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact
     2641 gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat
     2701 gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact
     2761 tggcagtaca tctacgtatt agtcatcgct attaccatgg tcgaggtgag ccccacgttc
     2821 tgcttcactc tccccatctc ccccccctcc ccacccccaa ttttgtattt atttattttt
     2881 taattatttt gtgcagcgat gggggcgggg gggggggggg ggcccccccc aggcggggcg
     2941 gggcggggcg aggggcgggg cggggcgagg cggaaaggtg cggcggcagc caatcagagc
     3001 ggcgcgctcc gaaagtttcc ttttatggcg aggcggcggc ggcggcggcc ctataaaaag
     3061 cgaagcgcgc ggcgggcggg agtcgttgcg cgctgccttc cccccgtgcc ccgctccgcc
     3121 gccgcctcgc gccgcccgcc ccggctctga ctgaccgcgt tactcccaca ggtgagcggg
     3181 cgggacggcc cttctcctcc gggctgtaat tagcgcttgg tttaatgacg gcttgtttct
     3241 tttctgtggc tgcgtgaaag ccttgagggg ctccgggagg gccctttgtg cggggggagc
     3301 ggctcggggg gtgcgtgcgt gtgtgtgtgc gtggggagcg ccgcgtgcgg ctccgcgctg
     3361 cccggcggct gtgagcgctg cgggcgcggc gcggggcttt gtgcgctccg cagtgtgcgc
     3421 gaggggagcg cggccggggg cggtgccccg cggtgcgggg ggggctgcga ggggaacaaa
     3481 ggctgcgtgc ggggtgtgtg cgtggggggg tgagcagggg gtgtgggcgc gtcggtcggg
     3541 ctgcaacccc ccctgcaccc ccctccccga gttgctgagc acggcccggc ttcgggtgcg
     3601 gggctccgta cggggcgtgg cgcggggctc gccgtgccgg gcggggggtg gcggcaggtg
     3661 ggggtgccgg gcggggcggg gccgcctcgg gccggggagg gctcggggga aggggcgcgg
     3721 cggcccccgg agcgccggcg gctgtcgagg cgcggcgagc cgcagccatt gccttttatg
     3781 gtaatcgtgc gagagggcgc agggacttcc tttgtcccaa atctgtgcgg agccgaaatc
     3841 tgggaggcgc cgccgcaccc cctctagcgg gcgcggggcg aagcggtgcg gcgccggcag
     3901 gaaggaaatg ggcggggagg gccttcgtgc gtcgccgcgc cgccgtcccc ttctccctct
     3961 ccagcctcgg ggctgtccgc ggggggacgg ctgccttcgg gggggacggg gcagggcggg
     4021 gttcggcttc tggcgtgtga ccggcggctc tagagcctct gctaaccatg ttcatgcctt
     4081 cttctttttc ctacagctcc tgggcaacgt gctggttatt gtgctgtctc atcattttgg
     4141 caaagaattc taactttaaa taattggcat tatttaaagt taagaagttc ctattctcta
     4201 gaaagtatag gaacttcgtt gacaattaat catcggcata gtatatcggc atagtataat
     4261 acgacaaggt gaggaactaa accatgggat cggccattga acaagatgga ttgcacgcag
     4321 gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg
     4381 gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca
     4441 agaccgacct gtccggtgcc ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc
     4501 tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg
     4561 actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg
     4621 ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta
     4681 cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag
     4741 ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac
     4801 tgttcgccag gctcaaggcg cgcatgcccg acggcgatga tctcgtcgtg acccatggcg
     4861 atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg
     4921 gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg
     4981 aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg
     5041 attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgaggg gatcaattcg
     5101 aagttcctat tctctagaaa gtataggaac ttcgctagca gctcgctgat cagcctcgac
     5161 tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct
     5221 ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct
     5281 gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg
     5341 ggaagacaat agcaggcatg ctggggatgc ggtgggctct atggcttctg aggcggaaag
     5401 aaccagctgg ggctcgacta gagcttgcgg aacccttacc accgcggtgg cggccgctct
     5461 agaatcataa tcagccttac cacatttgta gaggttttac ttgctttaaa aaacctccca
     5521 cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa cttgtttatt
     5581 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
     5641 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta actagaatca
     5701 taatcagcca taccacattt gtagaggttt tacttgcttt aaaaaacctc ccacacctcc
     5761 ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt taacttgttt attgcagctt
     5821 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     5881 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttaactagaa tcataatcag
     5941 ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa
     6001 cctgaaacat aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg
     6061 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
     6121 tagttgtggt ttgtccaaac tcatcaatgt atcttaacta gtggatctca tgcctaggta
     6181 actttaaata attggcatta tttaaagtta ggcgcgccat ggtgagcaag ggcgaggagg
     6241 tcatcaaaga gttcatgcgc ttcaaggtgc gcatggaggg ctccatgaac ggccacgagt
     6301 tcgagatcga gggcgagggc gagggccgcc cctacgaggg cacccagacc gccaagctga
     6361 aggtgaccaa gggcggcccc ctgcccttcg cctgggacat cctgtccccc cagttcatgt
     6421 acggctccaa ggcgtacgtg aagcaccccg ccgacatccc cgattacaag aagctgtcct
     6481 tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga ggacggcggt ctggtgaccg
     6541 tgacccagga ctcctccctg caggacggca cgctgatcta caaggtgaag atgcgcggca
     6601 ccaacttccc ccccgacggc cccgtaatgc agaagaagac catgggctgg gaggcctcca
     6661 ccgagcgcct gtacccccgc gacggcgtgc tgaagggcga gatccaccag gccctgaagc
     6721 tgaaggacgg cggccactac ctggtggagt tcaagaccat ctacatggcc aagaagcccg
     6781 tgcaactgcc cggctactac tacgtggaca ccaagctgga catcacctcc cacaacgagg
     6841 actacaccat cgtggaacag tacgagcgct ccgagggccg ccaccacctg ttcctggggc
     6901 atggcaccgg cagcaccggc agcggcagct ccggcaccgc ctcctccgag gacaacaaca
     6961 tggccgtcat caaagagttc atgcgcttca aggtgcgcat ggagggctcc atgaacggcc
     7021 acgagttcga gatcgagggc gagggcgagg gccgccccta cgagggcacc cagaccgcca
     7081 agctgaaggt gaccaagggc ggccccctgc ccttcgcctg ggacatcctg tccccccagt
     7141 tcatgtacgg ctccaaggcg tacgtgaagc accccgccga catccccgat tacaagaagc
     7201 tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac ggcggtctgg
     7261 tgaccgtgac ccaggactcc tccctgcagg acggcacgct gatctacaag gtgaagatgc
     7321 gcggcaccaa cttccccccc gacggccccg taatgcagaa gaagaccatg ggctgggagg
     7381 cctccaccga gcgcctgtac ccccgcgacg gcgtgctgaa gggcgagatc caccaggccc
     7441 tgaagctgaa ggacggcggc cactacctgg tggagttcaa gaccatctac atggccaaga
     7501 agcccgtgca actgcccggc tactactacg tggacaccaa gctggacatc acctcccaca
     7561 acgaggacta caccatcgtg gaacagtacg agcgctccga gggccgccac cacctgttcc
     7621 tgtacggcat ggacgagctg tacaagtaat taattaaaac ctctggatta caaaatttgt
     7681 gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct
     7741 ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat
     7801 aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg
     7861 gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag
     7921 ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc
     7981 tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg
     8041 tcggggaaat catcgtcctt tccttggctg ctcgcctgtg ttgccacctg gattctgcgc
     8101 gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc
     8161 ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc
     8221 tccctttggg ccgcctcccc gcaggatcca ctagagcggc cgcctagagc tcgctgatca
     8281 gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc
     8341 ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg
     8401 cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg
     8461 gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag
     8521 gcggaaagaa ccagctgggg ctcgactaga gcttgcggaa cccttaagct taaagactgg
     8581 agttgcagat cacgagggaa gagggggaag ggattctccc aggcccaggg cggtcctcag
     8641 aagccaggag gcagcagaga actcccagaa aggtattgca acactcccct cccccctccg
     8701 gagaagggtg cggccttctc cccgcctact ccactgcagc tcccttactg ataacaactc
     8761 agagcgactt tgggagagca agtgctcgag ggggggcccg gtacccaatt cgccctatag
     8821 tgagtcgtat tacgcgcgct cactggccgt cgttttacaa cgtcgtgact gggaaaaccc
     8881 tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag
     8941 cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggaa
     9001 attgtaagcg ttaatatttt gttaaaattc gcgttaaatt tttgttaaat cagctcattt
     9061 tttaaccaat aggccgaaat cggcaaaatc ccttataaat caaaagaata gaccgagata
     9121 gggttgagtg ttgttccagt ttggaacaag agtccactat taaagaacgt ggactccaac
     9181 gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc atcaccctaa
     9241 tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc
     9301 cgatttagag cttgacgggg aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg
     9361 aaaggagcgg gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca
     9421 cccgccgcgc ttaatgcgcc gctacagggc gcgtcag
//
