LOCUS       Exported                9344 bp ds-DNA     circular SYN 28-OCT-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9344)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 28, 2018 from SnapGene Viewer 4.2.6
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9344
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    2134..2150
                     /label=M13R
                     /note="M13R"
     misc_feature    2136..2154
                     /label=M13 reverse priming site
                     /note="M13 reverse priming site"
     misc_feature    2177..2180
                     /label=Pme1 site
                     /note="Pme1 site"
     misc_feature    2185..2392
                     /label=5' homology arm
                     /note="5' homology arm"
     enhancer        2406..2783
                     /label=hCMV
                     /note="hCMV"
     misc_feature    complement(2729..2750)
                     /label=HN853
                     /note="HN853"
     promoter        2789..4130
                     /label=AG hybrid promoter
                     /note="AG hybrid promoter"
     intron          3158..4082
                     /note="intron"
     gene            complement(4137..4170)
                     /label=loxP
                     /note="loxP"
     gene            4172..4205
                     /label=FRT
                     /note="FRT"
     gene            4206..4271
                     /label=EM7 promoter
                     /note="EM7 promoter"
     misc_feature    4206..4237
                     /label=Untitled
     CDS             4272..5075
                     /label=Neo
                     /note="Neo"
     misc_feature    5063..5122
                     /label=Untitled
     misc_feature    5063..5087
                     /label=Untitled
     gene            5088..5121
                     /label=FRT
                     /note="FRT"
     misc_feature    5122
                     /label=Untitled
     misc_feature    5122
                     /label=Untitled
     gene            5128..5426
                     /label=bGHpA
                     /note="bGHpA"
     misc_feature    5451..5678
                     /label=SV40 pA
                     /note="SV40 pA"
     misc_feature    5684..5911
                     /label=SV40 pA
                     /note="SV40 pA"
     misc_feature    5917..6144
                     /label=SV40 pA
                     /note="SV40 pA"
     gene            complement(6167..6200)
                     /label=loxP
                     /note="loxP"
     misc_feature    6210..7640
                     /label=tdTomato
                     /note="tdTomato"
     misc_feature    6210..6230
                     /label=Untitled
     misc_feature    6210..6230
                     /label=HN921
                     /note="HN921"
     misc_feature    6873..6956
                     /label=linker
                     /note="linker"
     misc_feature    complement(6877..6894)
                     /label=Untitled
     misc_feature    6902
                     /label=c to t Age1
                     /note="c to t Age1"
     misc_feature    complement(7617..7640)
                     /label=Untitled
     misc_feature    complement(7619..7640)
                     /label=HN304
                     /note="HN304"
     misc_feature    7649..8234
                     /label=WPRE
     misc_feature    8255..8556
                     /label=bGH pA
                     /note="bGH pA"
     misc_feature    8573..8686
                     /label=3' homology arm
                     /note="3' homology arm"
     misc_feature    complement(8699..8720)
                     /label=T7 priming site
                     /note="T7 priming site"
     misc_feature    complement(8729..8745)
                     /label=M13 F2 priming site
                     /note="M13 F2 priming site"
     misc_feature    complement(8729..8745)
                     /label=M13F
                     /note="M13F"
ORIGIN
        1 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
       61 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
      121 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
      181 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
      241 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
      301 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
      361 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
      421 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
      481 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
      541 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
      601 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
      661 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
      721 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
      781 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
      841 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
      901 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
      961 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     1021 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     1081 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     1141 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     1201 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     1261 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc
     1321 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     1381 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     1441 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     1501 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     1561 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     1621 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     1681 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     1741 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     1801 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     1861 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     1921 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     1981 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
     2041 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
     2101 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg
     2161 ccaagcgcgc aattaagttt aaacaagggc aggatgtgtc aaactgccaa tagagaacta
     2221 cttactcttc aggctgaagc tgatggaaca ggtaacaaag gcaaacacta atcatgatca
     2281 gcaagatgaa gcagaaaggg aacaagggga tattaaatgt gtatagacac gctagagaga
     2341 tggctcagca gttaagagaa ctagctggtc tttcagaggt cctgagatca ataccggtca
     2401 tggtcgacat tgattattga ctagttatta atagtaatca attacggggt cattagttca
     2461 tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc
     2521 gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat
     2581 agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt
     2641 acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc
     2701 cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta
     2761 cgtattagtc atcgctatta ccatggtcga ggtgagcccc acgttctgct tcactctccc
     2821 catctccccc ccctccccac ccccaatttt gtatttattt attttttaat tattttgtgc
     2881 agcgatgggg gcgggggggg ggggggggcc ccccccaggc ggggcggggc ggggcgaggg
     2941 gcggggcggg gcgaggcgga aaggtgcggc ggcagccaat cagagcggcg cgctccgaaa
     3001 gtttcctttt atggcgaggc ggcggcggcg gcggccctat aaaaagcgaa gcgcgcggcg
     3061 ggcgggagtc gttgcgcgct gccttccccc cgtgccccgc tccgccgccg cctcgcgccg
     3121 cccgccccgg ctctgactga ccgcgttact cccacaggtg agcgggcggg acggcccttc
     3181 tcctccgggc tgtaattagc gcttggttta atgacggctt gtttcttttc tgtggctgcg
     3241 tgaaagcctt gaggggctcc gggagggccc tttgtgcggg gggagcggct cggggggtgc
     3301 gtgcgtgtgt gtgtgcgtgg ggagcgccgc gtgcggctcc gcgctgcccg gcggctgtga
     3361 gcgctgcggg cgcggcgcgg ggctttgtgc gctccgcagt gtgcgcgagg ggagcgcggc
     3421 cgggggcggt gccccgcggt gcgggggggg ctgcgagggg aacaaaggct gcgtgcgggg
     3481 tgtgtgcgtg ggggggtgag cagggggtgt gggcgcgtcg gtcgggctgc aaccccccct
     3541 gcacccccct ccccgagttg ctgagcacgg cccggcttcg ggtgcggggc tccgtacggg
     3601 gcgtggcgcg gggctcgccg tgccgggcgg ggggtggcgg caggtggggg tgccgggcgg
     3661 ggcggggccg cctcgggccg gggagggctc gggggaaggg gcgcggcggc ccccggagcg
     3721 ccggcggctg tcgaggcgcg gcgagccgca gccattgcct tttatggtaa tcgtgcgaga
     3781 gggcgcaggg acttcctttg tcccaaatct gtgcggagcc gaaatctggg aggcgccgcc
     3841 gcaccccctc tagcgggcgc ggggcgaagc ggtgcggcgc cggcaggaag gaaatgggcg
     3901 gggagggcct tcgtgcgtcg ccgcgccgcc gtccccttct ccctctccag cctcggggct
     3961 gtccgcgggg ggacggctgc cttcgggggg gacggggcag ggcggggttc ggcttctggc
     4021 gtgtgaccgg cggctctaga gcctctgcta accatgttca tgccttcttc tttttcctac
     4081 agctcctggg caacgtgctg gttattgtgc tgtctcatca ttttggcaaa gaattcataa
     4141 cttcgtatag catacattat acgaagttat agaagttcct attctctaga aagtatagga
     4201 acttcgttga caattaatca tcggcatagt atatcggcat agtataatac gacaaggtga
     4261 ggaactaaac catgggatcg gccattgaac aagatggatt gcacgcaggt tctccggccg
     4321 cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg
     4381 ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt
     4441 ccggtgccct gaatgaactg caggacgagg cagcgcggct atcgtggctg gccacgacgg
     4501 gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat
     4561 tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat
     4621 ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg
     4681 accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg
     4741 atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc
     4801 tcaaggcgcg catgcccgac ggcgatgatc tcgtcgtgac ccatggcgat gcctgcttgc
     4861 cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg
     4921 tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg
     4981 gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca
     5041 tcgccttcta tcgccttctt gacgagttct tctgagggga tcaattcgaa gttcctattc
     5101 tctagaaagt ataggaactt cgctagcagc tcgctgatca gcctcgactg tgccttctag
     5161 ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac
     5221 tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca
     5281 ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag
     5341 caggcatgct ggggatgcgg tgggctctat ggcttctgag gcggaaagaa ccagctgggg
     5401 ctcgactaga gcttgcggaa cccttaccac cgcggtggcg gccgctctag aatcataatc
     5461 agccttacca catttgtaga ggttttactt gctttaaaaa acctcccaca cctccccctg
     5521 aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc agcttataat
     5581 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
     5641 tctagttgtg gtttgtccaa actcatcaat gtatcttaac tagaatcata atcagccata
     5701 ccacatttgt agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga
     5761 aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca
     5821 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     5881 gtggtttgtc caaactcatc aatgtatctt aactagaatc ataatcagcc ataccacatt
     5941 tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa
     6001 aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag
     6061 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
     6121 gtccaaactc atcaatgtat cttaactagt ggatctcatg cctaggataa cttcgtatag
     6181 catacattat acgaagttat aggcgcgcca tggtgagcaa gggcgaggag gtcatcaaag
     6241 agttcatgcg cttcaaggtg cgcatggagg gctccatgaa cggccacgag ttcgagatcg
     6301 agggcgaggg cgagggccgc ccctacgagg gcacccagac cgccaagctg aaggtgacca
     6361 agggcggccc cctgcccttc gcctgggaca tcctgtcccc ccagttcatg tacggctcca
     6421 aggcgtacgt gaagcacccc gccgacatcc ccgattacaa gaagctgtcc ttccccgagg
     6481 gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg tctggtgacc gtgacccagg
     6541 actcctccct gcaggacggc acgctgatct acaaggtgaa gatgcgcggc accaacttcc
     6601 cccccgacgg ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc accgagcgcc
     6661 tgtacccccg cgacggcgtg ctgaagggcg agatccacca ggccctgaag ctgaaggacg
     6721 gcggccacta cctggtggag ttcaagacca tctacatggc caagaagccc gtgcaactgc
     6781 ccggctacta ctacgtggac accaagctgg acatcacctc ccacaacgag gactacacca
     6841 tcgtggaaca gtacgagcgc tccgagggcc gccaccacct gttcctgggg catggcaccg
     6901 gcagcaccgg cagcggcagc tccggcaccg cctcctccga ggacaacaac atggccgtca
     6961 tcaaagagtt catgcgcttc aaggtgcgca tggagggctc catgaacggc cacgagttcg
     7021 agatcgaggg cgagggcgag ggccgcccct acgagggcac ccagaccgcc aagctgaagg
     7081 tgaccaaggg cggccccctg cccttcgcct gggacatcct gtccccccag ttcatgtacg
     7141 gctccaaggc gtacgtgaag caccccgccg acatccccga ttacaagaag ctgtccttcc
     7201 ccgagggctt caagtgggag cgcgtgatga acttcgagga cggcggtctg gtgaccgtga
     7261 cccaggactc ctccctgcag gacggcacgc tgatctacaa ggtgaagatg cgcggcacca
     7321 acttcccccc cgacggcccc gtaatgcaga agaagaccat gggctgggag gcctccaccg
     7381 agcgcctgta cccccgcgac ggcgtgctga agggcgagat ccaccaggcc ctgaagctga
     7441 aggacggcgg ccactacctg gtggagttca agaccatcta catggccaag aagcccgtgc
     7501 aactgcccgg ctactactac gtggacacca agctggacat cacctcccac aacgaggact
     7561 acaccatcgt ggaacagtac gagcgctccg agggccgcca ccacctgttc ctgtacggca
     7621 tggacgagct gtacaagtaa ttaattaaaa cctctggatt acaaaatttg tgaaagattg
     7681 actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct
     7741 ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg
     7801 ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact
     7861 gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc
     7921 gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc
     7981 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa
     8041 tcatcgtcct ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc
     8101 ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg
     8161 gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg
     8221 gccgcctccc cgcaggatcc actagagcgg ccgcctagag ctcgctgatc agcctcgact
     8281 gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg
     8341 gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg
     8401 agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg
     8461 gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctga ggcggaaaga
     8521 accagctggg gctcgactag agcttgcgga acccttaagc ttgtcattcg aatttagaca
     8581 cccacatggt ggctcatgac catctatcta taaatggatc tgattttcat gtctggcagt
     8641 gtacagaagc taactgaaga aaggtggaag acccacaaga gttcaaggta cccaattcgc
     8701 cctatagtga gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt cgtgactggg
     8761 aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc
     8821 gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg
     8881 aatggaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag
     8941 ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac
     9001 cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga
     9061 ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc
     9121 accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg
     9181 gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa
     9241 gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac
     9301 caccacaccc gccgcgctta atgcgccgct acagggcgcg tcag
//
