LOCUS       Exported                9897 bp ds-DNA     circular SYN 16-JAN-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9897)
  AUTHORS   Jane
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Dec 13, 2021 from SnapGene 4.3.6
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9897
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1686..1919
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    2446..2563
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    2466..2485
                     /label=??-F
     promoter        2643..2882
                     /label=U6 Promoter
                     /note="RNA polymerase III promoter for human U6 snRNA"
     misc_feature    2810..2833
                     /label=U6(human)
     misc_feature    2866..2890
                     /label=Caslib-F
     misc_feature    2893..2898
                     /label=BsmB1
     promoter        2914..2932
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    2962..3086
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     misc_feature    3133..3159
                     /label=Random-F1
     misc_feature    complement(3332..3361)
                     /label=Random-R
     CDS             complement(3515..3820)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     CDS             complement(4170..4823)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG"
     misc_feature    4387..4392
                     /label=BsmB1
     promoter        complement(4824..4926)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene"
     misc_feature    4928..4933
                     /label=BsmB1
     protein_bind    complement(5104..5228)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     misc_feature    5209..5238
                     /label=Junp-F
     misc_feature    5248..5253
                     /label=5` cut
     misc_feature    5254..5259
                     /label=BsmB1
     misc_feature    5278..5283
                     /label=3` cut (XhoI)
     primer_bind     5280..5296
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    5291..5317
                     /label=Junp-R
     protein_bind    5338..5371
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     misc_feature    complement(5367..5399)
                     /label=pcg2.0-R
     misc_feature    complement(5395..5414)
                     /label=Caslib-R
     enhancer        5487..5790
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5791..5994
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     misc_feature    6023..6028
                     /label=kozak
     misc_feature    6029..6730
                     /label=tagBFP
     misc_feature    6786..7374
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     misc_feature    complement(6907..6926)
                     /label=WPRE-R
     misc_feature    complement(6907..6926)
                     /label=??-R
     CDS             complement(7257..7268)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7377..7393)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     LTR             7903..8083
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      complement(8145..8733)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    8576..8582
                     /label=mis
     CDS             complement(8904..9764)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     misc_feature    9662..9667
                     /label=mis
     promoter        complement(9765..9869)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg gccgcgctga tcttcagacc tggaggagga
     1561 gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca
     1621 ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg
     1681 ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg
     1741 tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac
     1801 aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc
     1861 aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg
     1921 gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt
     1981 tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga
     2041 gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa
     2101 gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt
     2161 aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta
     2221 ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca
     2281 ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata
     2341 gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa cggatcggca
     2401 ctgcgtgcgc caattctgca gacaaatggc agtattcatc cacaatttta aaagaaaagg
     2461 ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca
     2521 aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga
     2581 cagcagagat ccagtttggt tagtaccggg cccgctctag aggtaccaag gtcgggcagg
     2641 aagagggcct atttccatga ttccttcata tttgcatata cgatacaagg ctgttagaga
     2701 gataattaga attaatttga ctgtaaacac aaagatatta gtacaaaata cgtgacgtag
     2761 aaagtaataa tttcttgggt atttgcagtt tttaaaatta tgttttaaaa tggactatca
     2821 tatgcttacc gtaacttgaa agtatttcga tttcttggct ttatatatct tgtggaaagg
     2881 acgaaacacc gagagacgct ggcttatcga aattaatacg actcactata gggagaccca
     2941 agctggctag ttaagctatc aacaagtttg tacaaaaaag ctgaacgaga aacgtaaaat
     3001 gatataaata tcaatatatt aaattagatt ttgcataaaa aacagactac ataatactgt
     3061 aaaacacaac atatccagtc actatgaatc aactacttag atggtattag tgacctgtag
     3121 tcgaccgaca gccttccaaa tgttcttcgg gtgatgctgc caacttagtc gaccgacagc
     3181 cttccaaatg ttcttctcaa acggaatcgt cgtatccagc ctactcgcta ttgtcctcaa
     3241 tgccgtatta aatcataaaa agaaataaga aaaagaggtg cgagcctctt ttttgtgtga
     3301 caaaataaaa acatctacct attcatatac gctagtgtca tagtcctgaa aatcatctgc
     3361 atcaagaaca atttcacaac tcttatactt ttctcttaca agtcgttcgg cttcatctgg
     3421 attttcagcc tctatactta ctaaacgtga taaagtttct gtaatttcta ctgtatcgac
     3481 ctgcagactg gctgtgtata agggagcctg acatttatat tccccagaac atcaggttaa
     3541 tggcgttttt gatgtcattt tcgcggtggc tgagatcagc cacttcttcc ccgataacgg
     3601 agaccggcac actggccata tcggtggtca tcatgcgcca gctttcatcc ccgatatgca
     3661 ccaccgggta aagttcacgg gagactttat ctgacagcag acgtgcactg gccaggggga
     3721 tcaccatccg tcgcccgggc gtgtcaataa tatcactctg tacatccaca aacagacgat
     3781 aacggctctc tcttttatag gtgtaaacct taaactgcat ttcaccagcc cctgttctcg
     3841 tcagcaaaag agccgttcat ttcaataaac cgggcgacct cagccatccc ttcctgattt
     3901 tccgctttcc agcgttcggc acgcagacga cgggcttcat tctgcatggt tgtgcttacc
     3961 agaccggaga tattgacatc atatatgcct tgagcaactg atagctgtcg ctgtcaactg
     4021 tcactgtaat acgctgcttc atagcatacc tctttttgac atacttcggg tatacatatc
     4081 agtatatatt cttataccgc aaaaatcagc gcgcaaatac gcatactgtt atctggcttt
     4141 tagtaagccg gatccacgcg gcgtttacgc cccccctgcc actcatcgca gtactgttgt
     4201 aattcattaa gcattctgcc gacatggaag ccatcacaaa cggcatgatg aacctgaatc
     4261 gccagcggca tcagcacctt gtcgccttgc gtataatatt tgcccatggt gaaaacgggg
     4321 gcgaagaagt tgtccatatt ggccacgttt aaatcaaaac tggtgaaact cacccaggga
     4381 ttggctgaga cgaaaaacat attctcaata aaccctttag ggaaataggc caggttttca
     4441 ccgtaacacg ccacatcttg cgaatatatg tgtagaaact gccggaaatc gtcgtggtat
     4501 tcactccaga gcgatgaaaa cgtttcagtt tgctcatgga aaacggtgta acaagggtga
     4561 acactatccc atatcaccag ctcaccgtct ttcattgcca tacggaattc cggatgagca
     4621 ttcatcaggc gggcaagaat gtgaataaag gccggataaa acttgtgctt atttttcttt
     4681 acggtcttta aaaaggccgt aatatccagc tgaacggtct ggttataggt acattgagca
     4741 actgactgaa atgcctcaaa atgttcttta cgatgccatt gggatatatc aacggtggta
     4801 tatccagtga tttttttctc cattttagct tccttagctc ctgaaaatct cgataactca
     4861 aaaaatacgc ccggtagtga tcttatttca ttatggtgaa agttggaacc tcttacgtgc
     4921 cgatcaacgt ctcattttcg ccaaaagttg gcccagggct tcccggtatc aacagggaca
     4981 ccaggattta tttattctgc gaagtgatct tccgtcacag gtatttattc ggcgcaaagt
     5041 gcgtcgggtg atgctgccaa cttagtcgac tacaggtcac taataccatc taagtagttg
     5101 attcatagtg actggatatg ttgtgtttta cagtattatg tagtctgttt tttatgcaaa
     5161 atctaattta atatattgat atttatatca ttttacgttt ctcgttcagc tttcttgtac
     5221 aaagtggttg atctagaggg cccgcggttc gaacgtctct tgatcatatg gcgcgccctc
     5281 gaggtcgacg gtatcgataa gctcgcttca cgagattcca gcaggtcgag ggacctaata
     5341 acttcgtata gcatacatta tacgaagtta tattaagggt tccaagctta agcggccgcg
     5401 tggataaccg tattaccgcc atgcattagt tattaatagt aatcaattac ggggtcatta
     5461 gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc
     5521 tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg
     5581 ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg
     5641 gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa
     5701 tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     5761 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     5821 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     5881 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     5941 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctggttta
     6001 gtgaaccgtc agatccgcta gcgccaccat gagcgagctg attaaggaga acatgcacat
     6061 gaagctgtac atggagggca ccgtggacaa ccatcacttc aagtgcacat ccgagggcga
     6121 aggcaagccc tacgagggca cccagaccat gagaatcaag gtggtcgagg gcggccctct
     6181 ccccttcgcc ttcgacatcc tggctactag cttcctctac ggcagcaaga ccttcatcaa
     6241 ccacacccag ggcatccccg acttcttcaa gcagtccttc cctgagggct tcacatggga
     6301 gagagtcacc acatacgaag acgggggcgt gctgaccgct acccaggaca ccagcctcca
     6361 ggacggctgc ctcatctaca acgtcaagat cagaggggtg aacttcacat ccaacggccc
     6421 tgtgatgcag aagaaaacac tcggctggga ggccttcacc gagactctgt accccgctga
     6481 cggcggcctg gaaggcagaa acgacatggc cctgaagctc gtgggcggga gccatctgat
     6541 cgcaaacatc aagaccacat atagatccaa gaaacccgct aagaacctca agatgcctgg
     6601 cgtctactat gtggactaca gactggaaag aatcaaggag gccaacaacg agacctacgt
     6661 cgagcagcac gaggtggcag tggccagata ctgcgacctc cctagcaaac tggggcacaa
     6721 actcaattaa acatgtttaa gggttccggt tccactaggt acaattcgat atcaagctta
     6781 tcgataatca acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg
     6841 ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt
     6901 cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg
     6961 agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc
     7021 ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc
     7081 tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc
     7141 ggctgttggg cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc
     7201 tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg
     7261 ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc
     7321 gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcatcgat
     7381 accgtcgacc tcgatcgaga cctagaaaaa catggagcaa tcacaagtag caatacagca
     7441 gctaccaatg ctgattgtgc ctggctagaa gcacaagagg aggaggaggt gggttttcca
     7501 gtcacacctc aggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac
     7561 tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag acaagatatc
     7621 cttgatctgt ggatctacca cacacaaggc tacttccctg attggcagaa ctacacacca
     7681 gggccaggga tcagatatcc actgaccttt ggatggtgct acaagctagt accagttgag
     7741 caagagaagg tagaagaagc caatgaagga gagaacaccc gcttgttaca ccctgtgagc
     7801 ctgcatggga tggatgaccc ggagagagaa gtattagagt ggaggtttga cagccgccta
     7861 gcatttcatc acatggcccg agagctgcat ccggactgta ctgggtctct ctggttagac
     7921 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa
     7981 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag
     8041 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagcatgtg agcaaaaggc
     8101 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     8161 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     8221 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     8281 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     8341 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     8401 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     8461 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     8521 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     8581 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     8641 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     8701 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     8761 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     8821 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     8881 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
     8941 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
     9001 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
     9061 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
     9121 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
     9181 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
     9241 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
     9301 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
     9361 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
     9421 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
     9481 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
     9541 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
     9601 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
     9661 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
     9721 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
     9781 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
     9841 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgac
//
