LOCUS       pCasper4-Pnos-NoNLS-PC 10830 bp ds-DNA     circular     17-AUG-2020
DEFINITION  .
FEATURES             Location/Qualifiers
     primer          complement(1108..1127)
                     /label="1.93-whiteSeq2"
                     /note="sequence: GCGTGAGAACTGCGACCCAC"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     primer          complement(1380..1404)
                     /label="1.90-P2PSeqCDSUp1"
                     /note="sequence: GCCATCAATTAAACACAAAGTGCTG"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     primer          complement(1412..1433)
                     /label="1.91-P2PSeqCDSUp2"
                     /note="sequence: GTTTCGTGACGAAGCTCCAAGC"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     primer          1574..1593
                     /label="28.51-vasa pro seq_Fwd"
                     /note="sequence: gtggaatgtcattttgagtg"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     primer          1618..1637
                     /label="44.14-pCasper_fwd_PrSeq"
                     /note="sequence: cttcaaatacccttggatcg"
                     /ApEinfo_revcolor=#f7977a
                     /ApEinfo_fwdcolor=#f7977a
     primer          1759..1780
                     /label="2.66-pcasper pnos seq F"
                     /note="sequence: ctagaattcggtacccggggat"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer          1782..1798
                     /label="1.3-P2PSeq1"
                     /note="sequence: tcgaggtcgacggtatc"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     primer          complement(1784..1801)
                     /label="1.74-P2P-GibsonUp"
                     /note="sequence: ATCGATACCGTCGACCTC"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     primer          complement(1838..1858)
                     /label="2.12-MCPGFPSeq4"
                     /note="sequence: GTTCGCTCACATCCGTCCTTA"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     promoter        1878..2733
                     /label="Pnos"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     primer          complement(2479..2502)
                     /label="2.40-pNOSSeqUp"
                     /note="sequence: AGGAATTTTGTGGTAAAGCTACGC"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     primer          2563..2581
                     /label="42.10_Nanos_fw_seq"
                     /note="sequence: cctctgcgtcacgatttta"
                     /ApEinfo_revcolor=#f7977a
                     /ApEinfo_fwdcolor=#f7977a
     primer          2636..2664
                     /label="2.11-MCPGFPSeq3"
                     /note="sequence: GAATACTTCAGTTGAATAAACTGTGCTTG"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     CDS             2748..3845
                     /label="Translation 2748-3845"
     CDS             2751..3119
                     /label="PCP"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    2783..2804
                     /label="Primer 8.19"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     CDS             3126..3845
                     /label="EGFP"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     primer          complement(3171..3192)
                     /label="EGFP-N"
                     /note="sequence: CGTCGCCGTCCAGCTCGACCAG"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     primer          3178..3196
                     /label="5.2-SYFPSeqInwardsUp"
                     /note="sequence: AGCTGGACGGCGACGTAAA"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     primer          complement(3178..3196)
                     /label="5.1-SYFPSeqUp"
                     /note="sequence: TTTACGTCGCCGTCCAGCT"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer          complement(3254..3275)
                     /label="2.17-pUBC-YFPSeq"
                     /note="sequence: GGTGCAGATGAACTTCAGGGTC"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     primer          complement(3374..3393)
                     /label="4.10-pCaSpeR4Seq3"
                     /note="sequence: GCATGGCGGACTTGAAGAAG"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     primer          complement(3504..3524)
                     /label="2.37-GFP-SeqUp"
                     /note="sequence: CTCCTTGAAGTCGATGCCCTT"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer          3536..3554
                     /label="3.2-eGFPBcdSeq2"
                     /note="sequence: CCTGGGGCACAAGCTGGAG"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     primer          3595..3615
                     /label="5.15-MissExpSeq6"
                     /note="sequence: AGCAGAAGAACGGCATCAAGG"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer          3772..3790
                     /label="5.4-SYFPSeqDown"
                     /note="sequence: GCGATCACATGGTCCTGCT"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    complement(3772..3790)
                     /label="Primer 8.21"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     primer          complement(3772..3790)
                     /label="5.3-SYFPSeqInwardsDw"
                     /note="sequence: AGCAGGACCATGTGATCGC"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     primer          3779..3800
                     /label="EGFP-C"
                     /note="sequence: CATGGTCCTGCTGGAGTTCGTG"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     primer          complement(3822..3863)
                     /label="12.25-PCPCVenusDw"
                     /note="sequence: cgcttagtgtgccgcggtttaCTTGTACAGCTCGTCCATGCC"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     primer          complement(3823..3845)
                     /label="GFP_FtzHomoR"
                     /note="sequence: ttacttgtacagctcgtccatgc"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer          complement(3823..3845)
                     /label="2.10-MCPGFPSeq2"
                     /note="sequence: TTACTTGTACAGCTCGTCCATGC"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     primer          3827..3872
                     /label="2.22-pNOS-NotIDw2SacIIv2"
                     /note="sequence: GGACGAGCTGTACAAGTAAACCGCGGCACACTAAGCGTCGCGCCAC"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    3853..4668
                     /label="Tub3'UTR"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     primer          complement(3868..3887)
                     /label="1.9-P2PSeq5"
                     /note="sequence: CCATCGAGCGTTGAAGTGGC"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     primer          complement(3908..3932)
                     /label="2.43-MCP-xFPSeqDw"
                     /note="sequence: CGTAAACACTGATTTCGACGGTTAC"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer          complement(3931..3949)
                     /label="16.28-pIBNOMS2_R12"
                     /note="sequence: tttgttgcgattggaagcg"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     primer          complement(3942..3965)
                     /label="16.24-pIBNoMSII-R10"
                     /note="sequence: gtgttgcagtgaattttttgttgc"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     primer          complement(3955..3978)
                     /label="1.26-P2PSeq10"
                     /note="sequence: CGTATGCTTTTCAGTGTTGCAGTG"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     primer          complement(3962..3985)
                     /label="16.27-pIBNoMS2_R11"
                     /note="sequence: tcgttttcgtatgcttttcagtgt"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     primer          complement(3977..4000)
                     /label="2.36-Tub3-SeqUpstream"
                     /note="sequence: CTCGTACAATCTTCATCGTTTTCG"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     primer          4220..4240
                     /label="1.43-P2P38F1Down3"
                     /note="sequence: AGAGCTGCCGTTCACTTCGTC"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     primer          complement(4250..4271)
                     /label="3.32-TubUpSeq1"
                     /note="sequence: GAATCGGGGAAAATAGAAAGCG"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     primer          4287..4306
                     /label="16.16-pIBNoMS2_II_F_1"
                     /note="sequence: ttttccgcctgctgtttgtg"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     primer          4299..4318
                     /label="16.20-pIBnoMS2_II_F3"
                     /note="sequence: tgtttgtggcaagtgtagca"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     primer          4308..4326
                     /label="16.18-pIBNoMS2_II_F2"
                     /note="sequence: caagtgtagcagcaggctg"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer          4628..4652
                     /label="2.9-MCPGFPSeq1"
                     /note="sequence: CCCCATAAATCTGAAACAATTAGGC"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     primer          complement(4673..4689)
                     /label="M13Forward"
                     /note="sequence: GTAAAACGACGGCCAGT"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     primer          complement(4680..4703)
                     /label="4.4-pPacLucSeq1"
                     /note="sequence: CCAGTCACGACGTTGTAAAACGAC"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer          complement(4686..4703)
                     /label="16.32-PP7TSeq4"
                     /note="sequence: ccagtcacgacgttgtaa"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     primer          complement(4700..4717)
                     /label="1.11-P2PSeq7"
                     /note="sequence: ACGCCAGGGTTTTCCCAG"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     primer          complement(4704..4720)
                     /label="16.31-PP7TSeq3"
                     /note="sequence: gtaacgccagggttttc"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer          complement(4715..4736)
                     /label="4.11-pPacLucSeq3"
                     /note="sequence: AAGGCGATTAAGTTGGGTAACG"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     primer          complement(4718..4738)
                     /label="16.22-BoxBLoops_F1"
                     /note="sequence: gcaaggcgattaagttgggta"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     primer          complement(4725..4746)
                     /label="2.13-MCPGFPSeq5"
                     /note="sequence: GATGTGCTGCAAGGCGATTAAG"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    complement(4867..5099)
                     /label="P\element\3'\end"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     primer          complement(4966..4990)
                     /label="4.6-pCaSpeR4Seq1"
                     /note="sequence: GAGTGAGACAGCGATATGATTGTTG"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     primer          4972..4996
                     /label="3.29-Carnegie4Seq4"
                     /note="sequence: TCATATCGCTGTCTCACTCAGACTC"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    complement(5100..5600)
                     /label="White\linker\sequence"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     primer          complement(5131..5155)
                     /label="3.20-Carnegie4Seq2"
                     /note="sequence: AAGTCTTAGAGCCAGATATGCGAGC"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     primer          5226..5249
                     /label="1.34-pUASTP2SeqDown6"
                     /note="sequence: CATCGTGTTTACTGTTTATTGCCC"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     primer          5566..5586
                     /label="misread"
                     /note="sequence: cttggagcgactgtcgttagt"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     primer          5755..5777
                     /label="pGEXReverse"
                     /note="sequence: CCGGGAGCTGCATGTGTCAGAGG"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     primer          complement(5816..5841)
                     /label="14.1-eveSeqUp1"
                     /note="sequence: cctataaaaataggcgtatcacgagg"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     primer          complement(5933..5957)
                     /label="14.2-eveSeqUp2"
                     /note="sequence: CATGAGCGGATACATATTTGAATGT"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     CDS             6006..6863
                     /label="ampR"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer          complement(6070..6089)
                     /label="3.31-Carnegie4Seq6"
                     /note="sequence: CAGCGTTTCTGGGTGAGCAA"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer          6565..6589
                     /label="PCSF"
                     /note="sequence: cgttgcgcaaactattaactggcga"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     primer          6565..6589
                     /label="PCSF"
                     /note="sequence: cgttgcgcaaactattaactggcga"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     primer          6565..6589
                     /label="PCSF"
                     /note="sequence: cgttgcgcaaactattaactggcga"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     primer          complement(6583..6609)
                     /label="35.122-Runt-C-Rfrag-Fwd"
                     /note="sequence: gggaagctagagtaagtagttcgccag"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer          complement(6585..6609)
                     /label="PCSR"
                     /note="sequence: gggaagctagagtaagtagttcgcc"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     primer          complement(6585..6609)
                     /label="PCSR"
                     /note="sequence: gggaagctagagtaagtagttcgcc"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     primer          complement(6585..6609)
                     /label="PCSR"
                     /note="sequence: gggaagctagagtaagtagttcgcc"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     primer          6794..6817
                     /label="3.30-Carnegie4Seq5"
                     /note="sequence: GAGTCAGGCAACTATGGATGAACG"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer          7154..7172
                     /label="10.84-pIB-amp-seq-fwd"
                     /note="sequence: cttcagcagagcgcagata"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     primer          complement(7154..7172)
                     /label="10.83-pIB-seq-rev"
                     /note="sequence: tatctgcgctctgctgaag"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     primer          7647..7687
                     /label="1.84-whiteUTRGibsonUp"
                     /note="sequence: ggctcggtgccagtataCCTCAAATGGTTGTCGTACCTCTC"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     primer          7748..7769
                     /label="1.96-whiteSeq5"
                     /note="sequence: CGAAATTAGCTGCACATCGTCG"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     primer          7785..7805
                     /label="3.19-Carnegie4Seq1"
                     /note="sequence: AGTTCGGGCAAGGTCATCCTG"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     primer          7787..7805
                     /label="5.11-pGXSeq3"
                     /note="sequence: TTCGGGCAAGGTCATCCTG"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     primer          complement(7937..7959)
                     /label="3.28-Carnegie4Seq3"
                     /note="sequence: CACAACCTTTCCTCTCAACAAGC"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     primer          8430..8450
                     /label="3.41-BcdGFP-DHFR-Up"
                     /note="sequence: CGTTTAGAGCAGCAGCCGAAT"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer          8454..8478
                     /label="1.27pUASTP2SeqUp1"
                     /note="sequence: TTCTAGTTCCAGTGAAATCCAAGCA"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     primer          8533..8554
                     /label="1.22attP3831SeqUp"
                     /note="sequence: AACAACACTATTATGCCCACCA"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     primer          8547..8568
                     /label="1.28pUASTP2SeqUp2"
                     /note="sequence: GCCCACCATTTTTTTGAGATGC"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     primer          complement(8784..8804)
                     /label="1.33-pUASTP2SeqDown5"
                     /note="sequence: ATTCGGTTCGCTCAAATGGTT"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     primer          complement(8813..8833)
                     /label="misread"
                     /note="sequence: cttggagcgactgtcgttagt"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     primer          complement(9150..9173)
                     /label="1.34-pUASTP2SeqDown6"
                     /note="sequence: CATCGTGTTTACTGTTTATTGCCC"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     primer          9244..9268
                     /label="3.20-Carnegie4Seq2"
                     /note="sequence: AAGTCTTAGAGCCAGATATGCGAGC"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     primer          complement(9346..9364)
                     /label="5.11-pGXSeq3"
                     /note="sequence: TTCGGGCAAGGTCATCCTG"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     primer          complement(9346..9366)
                     /label="3.19-Carnegie4Seq1"
                     /note="sequence: AGTTCGGGCAAGGTCATCCTG"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     primer          complement(9382..9403)
                     /label="1.96-whiteSeq5"
                     /note="sequence: CGAAATTAGCTGCACATCGTCG"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     primer          complement(9464..9504)
                     /label="1.84-whiteUTRGibsonUp"
                     /note="sequence: ggctcggtgccagtataCCTCAAATGGTTGTCGTACCTCTC"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     primer          9902..9926
                     /label="1.95-whiteSeq4"
                     /note="sequence: ACTTACATTTATCGTGGCAAAGACG"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     primer          complement(9984..10004)
                     /label="1.92-whiteSeq1"
                     /note="sequence: CCAACAACTCACGCAAGTGGG"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
ORIGIN
        1 ggaggtgggc tcatcgcaga tcagaagcgg cggatcggtt agtgcctcgg aggcgaatgc
       61 cagacgcttc ctttctccgc cggacagacc tttcaccctg ccgggcacac cgatgatcgt
      121 gtgctgacat ttgctgagcg aaagctcctg gatcacctga tccacgcggg ccactcgctg
      181 ccgataggtc agatgtcgtg gcatccgcac catggcctgg aaaatcaggt gttccctggc
      241 cgttagggag ccgataaaga ggtcatcctg ctggacatag gcgcacctgg cctgcatctc
      301 cttggcgtcc acaggttggc cattgagcag tcgcatcccg gatggcgata cttggatgcc
      361 ctgcggcgat cgaaaggcaa gggcattcag cagggtcgtc tttccggcac cggaactgcc
      421 catcacggcc aaaagttcgc ccggataggc cacgccgcaa actgagtttc aaattggtaa
      481 ttggaccctt tattaagatt tcacacagat cagccgactg cgaatagaaa ctcaccgttc
      541 ttgagcaaat gtttcctggg cgccggtatg tgtcgctcgt tgcagaatag tccgcgtgtc
      601 cggttgacca gctgccgcca tccggagccc ggctgattga ccgccccaaa gatgtccata
      661 ttgtgccagg cataggtgag gttctcggct agttggccgc tccctgaacc ggagtcctcc
      721 ggcggactgg gtggcaggag cgtgccgtag tttttggcct gcccgaagcc ctggttaatg
      781 cagctctgcg aagccgctcc gctgtcaccc tgcaatgata ggggatctca aatatcaact
      841 acaagcgtta tgctcatcta accccgaaca aaacgaagta tcctacgaag taggtttata
      901 cttttattta ttttttgtgc atctaggatc agcttaaaat atctggttgt tatatttttt
      961 gtaaaaaaga atgtagtcga aaatgaatgc ctttagatgt cttgatcatg atatgatctt
     1021 aaaaattgtc ttatatagcg agcacagcta ccagaataat ctgtttcgtg tcactatttg
     1081 tttgtgcgat tgcggtttgg gatttttgtg ggtcgcagtt ctcacgccgc agacaatttg
     1141 atgttgcaat cgcagttcct atagatcaag tgaacttaag atgtatgcac atgtactact
     1201 cacattgttc agatgctcgg cagatgggtg tttgctgcct ccgcgaatta atagctcctg
     1261 atcctcttgg cccattgccg ggatttttca cactttcccc tgcttaccca cccaaaacca
     1321 atcaccaccc caatcactca aaaaacaaac aaaaataaga agcgagagga gttttggcac
     1381 agcactttgt gtttaattga tggcgtaaac cgcttggagc ttcgtcacga aaccgctgac
     1441 aaagtgcaac tgaaggcgga cattgacgct aggtaacgct acaaacggtg gcgaaagaga
     1501 tagcggacgc agcggcgaaa gagacggcga tatttctgtg gacagagaag gaggcaaaca
     1561 gcgctgactt tgagtggaat gtcattttga gtgagaggta atcgaaagaa cctggtactt
     1621 caaataccct tggatcgaag taaatttaaa actgatcaga taagttcaat gatgtccagt
     1681 gcagtaaaaa ataaaaaaaa aatatgtttt tttaaatcta cattctccaa aaaagggttt
     1741 tattaactta catacatact agaattcggt acccggggat ctcgaggtcg acggtatcga
     1801 taagcttcga ccgttttaac ctcgaaatat gcacatgtaa ggacggatgt gagcgaacgc
     1861 cagtgatgac cgggatcaga ggtaacctac catggtgggg attaggtgac cgttcgcagg
     1921 tagtttgatc ggagcgaatg ttcggggggt ctggcgtcag aggctctaaa ctttatgtaa
     1981 ttcctgccgc gaaacacgca cgtatcaagc agtcagctgt tctcttcgtt cagcgcgcgc
     2041 cggtgttgca aaacgagcgc tcttcgccgg cggtggctcg tgcgatagtt cgttttgtcg
     2101 gtaatccgat gttgccgcgc cgatatcatg tgatgttgtc acagtgcgcg aaattcgaat
     2161 ggtggtgtgc agtgattgtg ttgtgacggc gagtggcgcg tgtgggtgct tagttttggg
     2221 agatgttttc gtattttttt gttgataact caggctttgt tgctgtgttg tagtactatt
     2281 ttccattgcg cggtgtccag cttttaatta gtggcacata ttcttagcaa gtaaaaatta
     2341 ttttgcatac tattaaattt cttataaatt attttctaaa attaagttta ccttttcaat
     2401 tttactaaaa atatcgatat atttattatc gctggaaaac tacattattc cacctctaag
     2461 caagaaccgt tagttggcgc gtagctttac cacaaaattc ctggaattgc cgtacgcttc
     2521 gcagttgttt caagttgtct aagggacata cgattttttt tgcctctgcg tcacgatttt
     2581 aacccaaaag cgagtttagt tacatgtaca ttattattag ataaagaagt atcgcgaata
     2641 cttcagttga ataaactgtg cttggttttt gggtgaggat ttgtggaaag tagagtgcgc
     2701 gataaccgta actttcgacc cggattttcc atagatcatg catcaccatg ctagcctcca
     2761 aaaccatcgt tctttcggtc ggcgaggcta ctcgcactct gactgagatc cagtccaccg
     2821 cagaccgtca gatcttcgaa gagaaggtcg ggcctctggt gggtcggctg cgcctcacgg
     2881 cttcgctccg tcaaaacgga gccaagaccg cgtatcgagt caacctaaaa ctggatcagg
     2941 cggacgtcgt tgattccgga cttccgaaag tgcgctacac tcaggtatgg tcgcacgacg
     3001 tgacaatcgt tgcgaatagc accgaggcct cgcgcaaatc gttgtacgat ttgaccaagt
     3061 ccctcgtcgc gacctcgcag gtcgaagatc ttgtcgtcaa ccttgtgccg ctgggccgtg
     3121 cggccgctgt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc
     3181 tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca
     3241 cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc
     3301 ccaccctcgt gaccaccctg acctacggcg tgcagtgctt cagccgctac cccgaccaca
     3361 tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca
     3421 tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca
     3481 ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg
     3541 ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcatggcc gacaagcaga
     3601 agaacggcat caaggtgaac ttcaagatcc gccacaacat cgaggacggc agcgtgcagc
     3661 tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca
     3721 accactacct gagcacccag tccgccctga gcaaagaccc caacgagaag cgcgatcaca
     3781 tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca
     3841 agtaaaccgc ggcacactaa gcgtcgcgcc acttcaacgc tcgatgggag cgtcattggt
     3901 gggcggggta accgtcgaaa tcagtgttta cgcttccaat cgcaacaaaa aattcactgc
     3961 aacactgaaa agcatacgaa aacgatgaag attgtacgag aaaccataaa gtattttatc
     4021 cacaaagaca cgtatagcag aaaagccaag ttaactcggc gataagttgt gtacacaaga
     4081 ataaaatcgg ccagattcag tgttgtcaga aataagaaaa ccccactatg tttttctttg
     4141 ccttttcttt ctcccagcga tcattcattt cgtggtgaaa gaacggggtc attgcacgga
     4201 gtttcgactg cgggaaagca gagctgccgt tcacttcgtc tataattagc gctttctatt
     4261 ttccccgatt cgggccgctg ctgcgctttt ccgcctgctg tttgtggcaa gtgtagcagc
     4321 aggctgtgca cgcagtgtgg catgcacttg gctttccacc gttggtatcg attctctggg
     4381 acgatgagtc attcctttcg gggccacagc ataatcgttg ccagctcacc gaaatggtga
     4441 cttcatttct taactgccgt caagcatgcg attgtacata catacatatt tatatatgta
     4501 catatttatg tgactatggt aggtcgatat aatagcaatc aacgcaagca aatgtgtcag
     4561 tcctgcttac aggaacgatt ctatttagta attttcgttg tataaagtaa ttatgtatgt
     4621 atgtaagccc cataaatctg aaacaattag gcaaaaccat gcgaagcttg gcactggccg
     4681 tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag
     4741 cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc
     4801 aacagttgcg cagcctgaat ggcgaatggc aactcgaggc ctcgaggtcg acctgcagcc
     4861 aagctttgcg tactcgcaaa ttattaaaaa taaaacttta aaaataattt cgtctaatta
     4921 atattatgag ttaattcaaa ccccacggac atgctaaggg ttaatcaaca atcatatcgc
     4981 tgtctcactc agactcaata cgacactcag aatactattc ctttcactcg cacttattgc
     5041 aagcatacgt taagtggatg tctcttgccg acgggaccac cttatgttat ttcatcatgg
     5101 tctggccatt ctcatcgtga gcttccgggt gctcgcatat ctggctctaa gacttcgggc
     5161 ccgacgcaag gagtagccga catatatccg aaataactgc ttgttttttt ttttaccatt
     5221 attaccatcg tgtttactgt ttattgcccc ctcaaaaagc taatgtaatt atatttgtgc
     5281 caataaaaac aagatatgac ctatagaata caagtatttc cccttcgaac atccccacaa
     5341 gtagactttg gatttgtctt ctaaccaaaa gacttacaca cctgcatacc ttacatcaaa
     5401 aactcgttta tcgctacata aaacaccggg atatattttt tatatacata cttttcaaat
     5461 cgcgcgccct cttcataatt cacctccacc acaccacgtt tcgtagttgc tctttcgctg
     5521 tctcccaccc gctctccgca acacattcac cttttgttcg acgaccttgg agcgactgtc
     5581 gttagttccg cgcgattcgg tgcggtattt cacaccgcat atggtgcact ctcagtacaa
     5641 tctgctctga tgccgcatag ttaagccagc cccgacaccc gccaacaccc gctgacgcgc
     5701 cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga
     5761 gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgagacga aagggcctcg
     5821 tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg
     5881 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
     5941 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
     6001 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
     6061 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
     6121 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
     6181 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
     6241 tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
     6301 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
     6361 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
     6421 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
     6481 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
     6541 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
     6601 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
     6661 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
     6721 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
     6781 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
     6841 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
     6901 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     6961 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     7021 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     7081 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     7141 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt
     7201 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     7261 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     7321 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     7381 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcat tgagaaagcg
     7441 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     7501 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     7561 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     7621 ggaaaaacgc cttcttcttg aactcgggct cggtgccagt atacctcaaa tggttgtcgt
     7681 acctctcatg gttccgttac gccaacgagg gtctgctgat taaccaatgg gcggacgtgg
     7741 agccgggcga aattagctgc acatcgtcga acaccacgtg ccccagttcg ggcaaggtca
     7801 tcctggagac gcttaacttc tccgccgccg atctgccgct ggactacgtg ggtctggccc
     7861 atgatgaaat aacataaggt ggtcccgtcg atagccgaag cttaccgaag tatacactta
     7921 aattcagtgc acgtttgctt gttgagagga aaggttgtgt gcggacgaat ttttttttga
     7981 aaacattaac ccttacgtgg aataaaaaaa aatgaaatat tgcaaatttt gctgcaaagc
     8041 tgtgactgga gtaaaattaa ttcacgtgcc gaagtgtgct attaagagaa aattgtggga
     8101 gcagagcctt gggtgcagcc ttggtgaaaa ctcccaaatt tgtgataccc actttaatga
     8161 ttcgcagtgg aaggctgcac ctgcaaaagg tcagacattt aaaaggaggc gactcaacgc
     8221 agatgccgta cctagtaaag tgatagagcc tgaaccagaa aagataaaag aaggctatac
     8281 cagtgggagt acacaaacag agtaagtttg aatagtaaaa aaaatcattt atgtaaacaa
     8341 taacgtgact gtgcgttagg tcctgttcat tgtttaatga aaataagagc ttgagggaaa
     8401 aaattcgtac tttggagtac gaaatgcgtc gtttagagca gcagccgaat taattctagt
     8461 tccagtgaaa tccaagcatt ttctaaatta aatgtattct tattattata gttgttattt
     8521 ttgatatata taaacaacac tattatgccc accatttttt tgagatgcat ctacacaagg
     8581 aacaaacact ggatgtcact ttcagttcaa attgtaacgc taatcactcc gaacaggtca
     8641 caaaaaatta ccttaaaaag tcataatatt aaattagaat aaatatagct gtgagggaaa
     8701 tatatacaaa tatattggag caaataaatt gtacatacaa atatttatta ctaatttcta
     8761 ttgagacgaa atgaaccact cggaaccatt tgagcgaacc gaatcgcgcg gaactaacga
     8821 cagtcgctcc aaggtcgtcg aacaaaaggt gaatgtgttg cggagagcgg gtgggagaca
     8881 gcgaaagagc aactacgaaa cgtggtgtgg tggaggtgaa ttatgaagag ggcgcgcgat
     8941 ttgaaaagta tgtatataaa aaatatatcc cggtgtttta tgtagcgata aacgagtttt
     9001 tgatgtaagg tatgcaggtg tgtaagtctt ttggttagaa gacaaatcca aagtctactt
     9061 gtggggatgt tcgaagggga aatacttgta ttctataggt catatcttgt ttttattggc
     9121 acaaatataa ttacattagc tttttgaggg ggcaataaac agtaaacacg atggtaataa
     9181 tggtaaaaaa aaaaacaagc agttatttcg gatatatgtc ggctactcct tgcgtcgggc
     9241 ccgaagtctt agagccagat atgcgagcac ccggaagctc acgatgagaa tggccagacc
     9301 cacgtagtcc agcggcagat cggcggcgga gaagttaagc gtctccagga tgaccttgcc
     9361 cgaactgggg cacgtggtgt tcgacgatgt gcagctaatt tcgcccggct ccacgtccgc
     9421 ccattggtta atcagcagac cctcgttggc gtaacggaac catgagaggt acgacaacca
     9481 tttgaggtat actggcaccg agcccgagtt caagaagaag ccgccaaaga gcaggaatgg
     9541 tatgataacc ggcggaccca cagacagcgc catcgaggtc gaggagctgg cgcaggatat
     9601 tagatatccg aaggacgttg acacattggc caccagagtg accagcgcca ggcagttgaa
     9661 gaagtgcagc actccggccc gcagtccgat catcggatag gcaatcgccg tgaagaccag
     9721 tggcactgtg agaaaaagcg gtaattcggc aatcgttttg cccagaaagt atgtgtcaca
     9781 gcgataaagt cgacttcggg cctccctcat aaaaactggc agctctgagg tgaacaccta
     9841 aatcgaatcg attcattaga aagttagtaa attattaata tgcaaatgta ttctaaacaa
     9901 gacttacatt tatcgtggca aagacgtttt gaaaggtcat gttggtcagg aagaggaaga
     9961 tggctccgtt gatattcatc acgcccactt gcgtgagttg ttggcccaaa aagatgaggc
    10021 caatcaagat ggcaaccatc tgcaaattaa aatgttactc gcatctcatt aatattcata
    10081 tcttcaacat gttcgcgagt taaatgaaat ttatttattt tctgcaaaac tataaactat
    10141 acatctcatt gaaaaaaact aagaagggtg tggaatcagg caattctaac taaaatctag
    10201 cgaatttgtt tccaagaatt gtaagcgtta tatcatttgt ttccactgga accactcacc
    10261 gttgtctgaa taagtcgcac ttttacgagg agtggttcct tgagcaccga cagccaggat
    10321 cgccacagga ccgcccggaa ctgcatgaac caggtggcct tgtaggtgta cccattctcc
    10381 ggctgctcca gtggcttctc caaatttttg gtggccaaca actgctccat atcccgggct
    10441 actttgctaa tagcaaaatt gtcgcatatc ttggcgatcc gatcacggga ctcgatctcc
    10501 cgtccgggca caacggccaa cacctgtacg taaaagtccg ccggattgta gttggtagga
    10561 cactgggcac ccacgctgga taggagttga gatgtaatgt aatgctagat acccttaata
    10621 aacacatcga actcactagg aaaagaagtc gacggcttcg ctgggagtgc ccaagaaagc
    10681 taccctgccc tcggccatca gaaggatctt gtcaaagagc tcaaacagct cggaagacgg
    10741 ctgatgaatg gtcaggatga cggtcttgcc cttctgcgac agcttcttca gcacctggac
    10801 gacgctgtgg gcggtaaatg agtccagtcc
//