LOCUS       Exported                2278 bp DNA     circular SYN 16-DEC-2021
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2278)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Jan 5, 2022 from SnapGene Viewer 5.3.0
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..2278
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /note="color: #ffffff"
     terminator      complement(9..105)
                     /label=AmpR Terminator
                     /note="color: #f58a5e; direction: LEFT"
     CDS             complement(106..963)
                     /label=AmpR
                     /note="color: #9f4240; direction: LEFT"
     promoter        complement(964..1094)
                     /label=AmpR Promoter
                     /note="color: #f58a5e; direction: LEFT"
     rep_origin      complement(1095..1858)
                     /direction=LEFT
                     /label=ColE1
                     /note="color: #7f7f7f"
     misc_feature    1886..2028
                     /label=ConL1
                     /label=Con1
                     /note="color: #ffef86"
     protein_bind    2029..2034
                     /label=BsmBI
                     /note="color: #b1ff67"
     misc_feature    2036..2060
                     /label=Con1 scar
                     /note="color: #ffef86"
     protein_bind    2036..2039
                     /label=BsmBI
                     /note="color: #b1ff67"
     misc_feature    2065..2101
                     /label=Spacer
                     /note="color: #c6c9d1"
     protein_bind    complement(2106..2109)
                     /label=BsmBI
                     /note="color: #b1ff67; direction: LEFT"
     protein_bind    complement(2111..2116)
                     /label=BsmBI
                     /note="color: #b1ff67; direction: LEFT"
     misc_feature    2117..2259
                     /label=ConR2
                     /label=Con2
                     /note="color: #ffef86"
ORIGIN
        1 gcggccgcga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
       61 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
      121 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
      181 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
      241 gcgggaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
      301 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
      361 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
      421 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
      481 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
      541 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
      601 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
      661 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
      721 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
      781 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
      841 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
      901 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
      961 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     1021 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
     1081 aaaagtgcca cctgtcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     1141 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     1201 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     1261 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
     1321 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     1381 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     1441 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     1501 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     1561 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     1621 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     1681 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     1741 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     1801 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtgc
     1861 ggccgcccct gaattcgcat ctagaacggg gtcatcacgg ctcatcatgc gccaaacaaa
     1921 tgtgtgcaat acacgctcgg atgactgcat gatgaccgca ctgactgggg acagcagatc
     1981 cacctaagcc tgtgagagaa gcagacaccc gacagatcaa ggcagttacg tctcaccaaa
     2041 ccagatgtca acacagctac aacgactaga gatctatgtg aggatcctaa ctcgagatcg
     2101 agctggatgt gagacgataa atcgcagcca agtgagtgaa tagatgacgc accacggtca
     2161 gacacggcca catcgtatct cacaggagca agcgcgatag gagcactcac acatagtacg
     2221 gtgatccgct gactcctttg cccaaataag acgtgagcca ctagtgcact gcagtaca
//
