LOCUS       Exported                5083 bp DNA     circular SYN 16-DEC-2021
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5083)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Jan 5, 2022 from SnapGene Viewer 5.3.0
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5083
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /note="color: #ffffff"
     protein_bind    complement(16..19)
                     /label=BsmBI
                     /note="color: #b1ff67; direction: LEFT"
     protein_bind    complement(21..26)
                     /label=BsmBI
                     /note="color: #b1ff67; direction: LEFT"
     misc_feature    27..169
                     /label=ConLS'
                     /label=ConS
                     /note="color: #ffef86"
     protein_bind    complement(175..180)
                     /label=BsaI
                     /note="color: #b1ff67; direction: LEFT"
     promoter        181..310
                     /label=BBa_J72163 GlpT Promoter
                     /note="color: #b1ff67"
     RBS             311..346
                     /label=sfGFP Ribosome Binding Site
                     /note="color: #b1ff67"
     CDS             347..1063
                     /label=sfGFP
                     /note="color: #b1ff67; direction: RIGHT"
     terminator      1064..1192
                     /label=BBa_B0015 Terminator
                     /note="color: #b1ff67"
     protein_bind    1193..1198
                     /label=BsaI
                     /note="color: #b1ff67"
     misc_feature    1204..1346
                     /label=ConRE'
                     /label=ConE
                     /note="color: #ffef86"
     protein_bind    1347..1352
                     /label=BsmBI
                     /note="color: #b1ff67"
     misc_feature    1354..1378
                     /label=ConE scar
                     /note="color: #ffef86"
     protein_bind    1354..1357
                     /label=BsmBI
                     /note="color: #b1ff67"
     promoter        1402..1778
                     /label=AgTEF Promoter
                     /note="color: #e8cdff"
     CDS             1781..2590
                     /label=KanR
                     /note="color: #cc99ff; direction: RIGHT"
     terminator      2591..2792
                     /label=AgTEF Terminator
                     /note="color: #e8cdff"
     rep_origin      2797..2913
                     /label=CEN6
                     /note="color: #d6b295"
     rep_origin      2914..3304
                     /label=ARS4
                     /note="color: #b4abac"
     terminator      complement(3317..3343)
                     /label=KanR Terminator
                     /note="color: #f58a5e; direction: LEFT"
     CDS             complement(3344..4159)
                     /label=KanR
                     /note="color: #9f4240; direction: LEFT"
     promoter        complement(4160..4307)
                     /label=KanR Promoter
                     /note="color: #f58a5e; direction: LEFT"
     rep_origin      complement(4308..5071)
                     /direction=LEFT
                     /label=ColE1
                     /note="color: #7f7f7f"
ORIGIN
        1 gaattcgcat ctagactgat gagacgtggt agagccacaa acagccggta caagcaacga
       61 tctccaggac catctgaatc atgcgcggat gacacgaact cacgacggcg atcacagaca
      121 ttaacccaca gtacagacac tgcgacaacg tggcaattcg tcgcaataca acgtgagacc
      181 gaaagtgaaa cgtgatttca tgcgtcattt tgaacatttt gtaaatctta tttaataatg
      241 tgtgcggcaa ttcacattta atttatgaat gttttcttaa catcgcggca actcaagaaa
      301 cggcaggttc ggatcttagc tactagagaa agaggagaaa tactagatgc gtaaaggcga
      361 agagctgttc actggtgtcg tccctattct ggtggaactg gatggtgatg tcaacggtca
      421 taagttttcc gtgcgtggcg agggtgaagg tgacgcaact aatggtaaac tgacgctgaa
      481 gttcatctgt actactggta aactgccggt tccttggccg actctggtaa cgacgctgac
      541 ttatggtgtt cagtgctttg ctcgttatcc ggaccatatg aagcagcatg acttcttcaa
      601 gtccgccatg ccggaaggct atgtgcagga acgcacgatt tcctttaagg atgacggcac
      661 gtacaaaacg cgtgcggaag tgaaatttga aggcgatacc ctggtaaacc gcattgagct
      721 gaaaggcatt gactttaaag aggacggcaa tatcctgggc cataagctgg aatacaattt
      781 taacagccac aatgtttaca tcaccgccga taaacaaaaa aatggcatta aagcgaattt
      841 taaaattcgc cacaacgtgg aggatggcag cgtgcagctg gctgatcact accagcaaaa
      901 cactccaatc ggtgatggtc ctgttctgct gccagacaat cactatctga gcacgcaaag
      961 cgttctgtct aaagatccga acgagaaacg cgatcatatg gttctgctgg agttcgtaac
     1021 cgcagcgggc atcacgcatg gtatggatga actgtacaaa tgaccaggca tcaaataaaa
     1081 cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct
     1141 ctctactaga gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta taggtctcag
     1201 ctggaaatct gctcgtcagt ggtgctcaca ctgacgaatc atgtacagat cataccgatg
     1261 actgcctggc gactcacaac taagcaagac agccggaacc agcgccggcg aacaccactg
     1321 catatatggc atatcacaac agtccacgtc tcaagcagtt acagagatgt tacgaaccac
     1381 tagtgcactg cagtacagtt tagcttgcct cgtccccgcc gggtcacccg gccagcgaca
     1441 tggaggccca gaataccctc cttgacagtc ttgacgtgcg cagctcaggg gcatgatgtg
     1501 actgtcgccc gtacatttag cccatacatc cccatgtata atcatttgca tccatacatt
     1561 ttgatggccg cacggcgcga agcaaaaatt acggctcctc gctccagacc tgcgagcagg
     1621 gaaacgctcc cctcacagac gcgttgaatt gtccccacgc cgcgcccctg tagagaaata
     1681 taaaaggtta ggatttgcca ctgaggttct tctttcatat acttcctttt aaaatcttgc
     1741 taggatacag ttctcacatc acatccgaac ataaacaaaa atgggtaagg aaaagactca
     1801 cgtttcgagg ccgcgattaa attccaacat ggatgctgat ttatatgggt ataaatgggc
     1861 tcgcgataat gtcgggcaat caggtgcgac aatctatcga ttgtatggga agcccgatgc
     1921 gccagagttg tttctgaaac atggcaaagg tagcgttgcc aatgatgtta cagatgagat
     1981 ggtcagacta aactggctga cggaatttat gcctcttccg accatcaagc attttatccg
     2041 tactcctgat gatgcatggt tactcaccac tgcgatcccc ggcaaaacag cattccaggt
     2101 attagaagaa tatcctgatt caggtgaaaa tattgttgat gcgctggcag tgttcctgcg
     2161 ccggttgcat tcgattcctg tttgtaattg tccttttaac agcgatcgcg tatttcgtct
     2221 ggctcaggcg caatcacgaa tgaataacgg tttggttgat gcgagtgatt ttgatgacga
     2281 gcgtaatggc tggcctgttg aacaagtctg gaaagaaatg cataagcttt tgccattctc
     2341 accggattca gtcgtcactc atggtgattt ctcacttgat aaccttattt ttgacgaggg
     2401 gaaattaata ggttgtattg atgttggacg agtcggaatc gcagaccgat accaggatct
     2461 tgccatccta tggaactgcc tcggtgagtt ttctccttca ttacagaaac ggctttttca
     2521 aaaatatggt attgataatc ctgatatgaa taaattgcag tttcatttga tgctcgatga
     2581 gtttttctaa agtaactgac aataaaaaga ttcttgtttt caagaacttg tcatttgtat
     2641 agttttttta tattgtagtt gttctatttt aatcaaatgt tagcgtgatt tatatttttt
     2701 ttcgcctcga catcatctgc ccagatgcga agttaagtgc gcagaaagta atatcatgcg
     2761 tcaatcgtat gtgaatgctg gtcgctatac tggagtatca cgtgctataa aaataattat
     2821 aatttaaatt ttttaatata aatatataaa ttaaaaatag aaagtaaaaa aagaaattaa
     2881 agaaaaaata gtttttgttt tccgaagatg taaaagactc tagggggatc gccaacaaat
     2941 actacctttt atcttgctct tcctgctctc aggtattaat gccgaattgt ttcatcttgt
     3001 ctgtgtagaa aaccacacac gaaaatcctg tgattttaca ttttacttat cgttaatcga
     3061 atgtatatct atttaatctg cttttcttgt ctaataaata tatatgtaaa gtacgctttt
     3121 tgttgaaatt ttttaaacct ttgtttattt ttttttcttc attccgtaac tcttctacct
     3181 tctttattta ctttctaaaa tccaaataca aaacataaaa ataaataaac acagagtaaa
     3241 ttcccaaatt attccatcat taaaagatac gaggcgcgtg taagttacag gcaagcgatc
     3301 cgtcccgagc ggccgcgtgt tacaaccaat taaccaattc tgattagaaa aactcatcga
     3361 gcatcaaatg aaactgcaat ttattcatat caggattatc aataccatat ttttgaaaaa
     3421 gccgtttctg taatgaagga gaaaactcac cgaggcagtt ccataggatg gcaagatcct
     3481 ggtatcggtc tgcgattccg actcgtccaa catcaataca acctattaat ttcccctcgt
     3541 caaaaataag gttatcaagt gagaaatcac catgagtgac gactgaatcc ggtgagaatg
     3601 gcaaaagctt atgcatttct ttccagactt gttcaacagg ccagccatta cgctcgtcat
     3661 caaaatcact cgcatcaacc aaaccgttat tcattcgtga ttgcgcctga gcgaggcgaa
     3721 atacgcgatc gctgttaaaa ggacaattac aaacaggaat cgaatgcaac cggcgcagga
     3781 acactgccag cgcatcaaca atattttcac ctgaatcagg atattcttct aatacctgga
     3841 atgctgtttt cccggggatc gcagtggtga gtaaccatgc atcatcagga gtacggataa
     3901 aatgcttgat ggtcggaaga ggcataaatt ccgtcagcca gtttagtctg accatctcat
     3961 ctgtaacatc attggcaacg ctacctttgc catgtttcag aaacaactct ggcgcatcgg
     4021 gcttcccata caatcgatag attgtcgcac ctgattgccc gacattatcg cgagcccatt
     4081 tatacccata taaatcagca tccatgttgg aatttaatcg cggcctggag caagacgttt
     4141 cccgttgaat atggctcata acaccccttg tattactgtt tatgtaagca gacagtttta
     4201 ttgttcatga tgatatattt ttatcttgtg caatgtaaca tcagagattt tgagacacaa
     4261 cgtggctttg ttgaataaat cgaacttttg ctgagttgaa ggatcagtca tgaccaaaat
     4321 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     4381 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     4441 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     4501 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
     4561 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     4621 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     4681 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     4741 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     4801 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     4861 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     4921 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     4981 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
     5041 tgcgttatcc cctgattctg tggataaccg tgcggccgcc cct
//
