LOCUS       Exported                5302 bp DNA     circular SYN 16-DEC-2021
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5302)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Jan 5, 2022 from SnapGene Viewer 5.3.0
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5302
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /note="color: #ffffff"
     protein_bind    complement(16..19)
                     /label=BsmBI
                     /note="color: #b1ff67; direction: LEFT"
     protein_bind    complement(21..26)
                     /label=BsmBI
                     /note="color: #b1ff67; direction: LEFT"
     misc_feature    27..169
                     /label=ConLS'
                     /label=ConS
                     /note="color: #ffef86"
     protein_bind    complement(175..180)
                     /label=BsaI
                     /note="color: #b1ff67; direction: LEFT"
     promoter        181..310
                     /label=BBa_J72163 GlpT Promoter
                     /note="color: #b1ff67"
     RBS             311..346
                     /label=sfGFP Ribosome Binding Site
                     /note="color: #b1ff67"
     CDS             347..1063
                     /label=sfGFP
                     /note="color: #b1ff67; direction: RIGHT"
     terminator      1064..1192
                     /label=BBa_B0015 Terminator
                     /note="color: #b1ff67"
     protein_bind    1193..1198
                     /label=BsaI
                     /note="color: #b1ff67"
     misc_feature    1204..1346
                     /label=ConRE'
                     /label=ConE
                     /note="color: #ffef86"
     protein_bind    1347..1352
                     /label=BsmBI
                     /note="color: #b1ff67"
     misc_feature    1354..1378
                     /label=ConE scar
                     /note="color: #ffef86"
     protein_bind    1354..1357
                     /label=BsmBI
                     /note="color: #b1ff67"
     promoter        1402..1778
                     /label=AgTEF Promoter
                     /note="color: #e8cdff"
     CDS             1781..2809
                     /label=HygR
                     /label=HPH (Hygromycin Phosphotransferase)
                     /note="color: #cc99ff; direction: RIGHT"
     terminator      2810..3011
                     /label=AgTEF Terminator
                     /note="color: #e8cdff"
     rep_origin      3016..3132
                     /label=CEN6
                     /note="color: #d6b295"
     rep_origin      3133..3523
                     /label=ARS4
                     /note="color: #b4abac"
     terminator      complement(3536..3562)
                     /label=KanR Terminator
                     /note="color: #f58a5e; direction: LEFT"
     CDS             complement(3563..4378)
                     /label=KanR
                     /note="color: #9f4240; direction: LEFT"
     promoter        complement(4379..4526)
                     /label=KanR Promoter
                     /note="color: #f58a5e; direction: LEFT"
     rep_origin      complement(4527..5290)
                     /direction=LEFT
                     /label=ColE1
                     /note="color: #7f7f7f"
ORIGIN
        1 gaattcgcat ctagactgat gagacgtggt agagccacaa acagccggta caagcaacga
       61 tctccaggac catctgaatc atgcgcggat gacacgaact cacgacggcg atcacagaca
      121 ttaacccaca gtacagacac tgcgacaacg tggcaattcg tcgcaataca acgtgagacc
      181 gaaagtgaaa cgtgatttca tgcgtcattt tgaacatttt gtaaatctta tttaataatg
      241 tgtgcggcaa ttcacattta atttatgaat gttttcttaa catcgcggca actcaagaaa
      301 cggcaggttc ggatcttagc tactagagaa agaggagaaa tactagatgc gtaaaggcga
      361 agagctgttc actggtgtcg tccctattct ggtggaactg gatggtgatg tcaacggtca
      421 taagttttcc gtgcgtggcg agggtgaagg tgacgcaact aatggtaaac tgacgctgaa
      481 gttcatctgt actactggta aactgccggt tccttggccg actctggtaa cgacgctgac
      541 ttatggtgtt cagtgctttg ctcgttatcc ggaccatatg aagcagcatg acttcttcaa
      601 gtccgccatg ccggaaggct atgtgcagga acgcacgatt tcctttaagg atgacggcac
      661 gtacaaaacg cgtgcggaag tgaaatttga aggcgatacc ctggtaaacc gcattgagct
      721 gaaaggcatt gactttaaag aggacggcaa tatcctgggc cataagctgg aatacaattt
      781 taacagccac aatgtttaca tcaccgccga taaacaaaaa aatggcatta aagcgaattt
      841 taaaattcgc cacaacgtgg aggatggcag cgtgcagctg gctgatcact accagcaaaa
      901 cactccaatc ggtgatggtc ctgttctgct gccagacaat cactatctga gcacgcaaag
      961 cgttctgtct aaagatccga acgagaaacg cgatcatatg gttctgctgg agttcgtaac
     1021 cgcagcgggc atcacgcatg gtatggatga actgtacaaa tgaccaggca tcaaataaaa
     1081 cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct
     1141 ctctactaga gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta taggtctcag
     1201 ctggaaatct gctcgtcagt ggtgctcaca ctgacgaatc atgtacagat cataccgatg
     1261 actgcctggc gactcacaac taagcaagac agccggaacc agcgccggcg aacaccactg
     1321 catatatggc atatcacaac agtccacgtc tcaagcagtt acagagatgt tacgaaccac
     1381 tagtgcactg cagtacagtt tagcttgcct cgtccccgcc gggtcacccg gccagcgaca
     1441 tggaggccca gaataccctc cttgacagtc ttgacgtgcg cagctcaggg gcatgatgtg
     1501 actgtcgccc gtacatttag cccatacatc cccatgtata atcatttgca tccatacatt
     1561 ttgatggccg cacggcgcga agcaaaaatt acggctcctc gctccagacc tgcgagcagg
     1621 gaaacgctcc cctcacagac gcgttgaatt gtccccacgc cgcgcccctg tagagaaata
     1681 taaaaggtta ggatttgcca ctgaggttct tctttcatat acttcctttt aaaatcttgc
     1741 taggatacag ttctcacatc acatccgaac ataaacaaaa atgggtaaaa agcctgaact
     1801 caccgcgacg tctgtcgaga agtttctgat cgaaaagttc gacagcgtgt ccgacctgat
     1861 gcagctctcg gagggcgaag aatctcgtgc tttcagcttc gatgtaggag ggcgtggata
     1921 tgtcctgcgg gtaaatagct gcgccgatgg tttctacaaa gatcgttatg tttatcggca
     1981 ctttgcatcg gccgcgctcc cgattccgga agtgcttgac attggggaat ttagcgagag
     2041 cctgacctat tgcatctccc gccgtgcaca gggtgtcacg ttgcaagacc tgcctgaaac
     2101 cgaactgccc gctgttctgc aaccggtcgc ggaggccatg gatgcgatcg ctgcggccga
     2161 tcttagccag acgagcgggt tcggcccatt cggaccgcaa ggaatcggtc aatacactac
     2221 atggcgtgat ttcatatgcg cgattgctga tccccatgtg tatcactggc aaactgtgat
     2281 ggacgacacc gtcagtgcgt ccgtcgcgca ggctctcgat gagctgatgc tttgggccga
     2341 ggactgcccc gaagtccggc acctcgtgca cgcggatttc ggctccaaca atgtcctgac
     2401 ggacaatggc cgcataacag cggtcattga ctggagcgag gcgatgttcg gggattccca
     2461 atacgaggtc gccaacatct tcttctggag gccgtggttg gcttgtatgg agcagcagac
     2521 gcgctacttc gagcggaggc atccggagct tgcaggatcg ccgcggctcc gggcgtatat
     2581 gctccgcatt ggtcttgacc aactctatca gagcttggtt gacggcaatt tcgatgatgc
     2641 agcttgggcg cagggtcgat gcgacgcaat cgtccgatcc ggagccggga ctgtcgggcg
     2701 tacacaaatc gcccgcagaa gcgcggccgt ctggaccgat ggctgtgtag aagtactcgc
     2761 cgatagtgga aaccgacgcc ccagcactcg tccgagggca aaggaataaa gtaactgaca
     2821 ataaaaagat tcttgttttc aagaacttgt catttgtata gtttttttat attgtagttg
     2881 ttctatttta atcaaatgtt agcgtgattt atattttttt tcgcctcgac atcatctgcc
     2941 cagatgcgaa gttaagtgcg cagaaagtaa tatcatgcgt caatcgtatg tgaatgctgg
     3001 tcgctatact ggagtatcac gtgctataaa aataattata atttaaattt tttaatataa
     3061 atatataaat taaaaataga aagtaaaaaa agaaattaaa gaaaaaatag tttttgtttt
     3121 ccgaagatgt aaaagactct agggggatcg ccaacaaata ctacctttta tcttgctctt
     3181 cctgctctca ggtattaatg ccgaattgtt tcatcttgtc tgtgtagaaa accacacacg
     3241 aaaatcctgt gattttacat tttacttatc gttaatcgaa tgtatatcta tttaatctgc
     3301 ttttcttgtc taataaatat atatgtaaag tacgcttttt gttgaaattt tttaaacctt
     3361 tgtttatttt tttttcttca ttccgtaact cttctacctt ctttatttac tttctaaaat
     3421 ccaaatacaa aacataaaaa taaataaaca cagagtaaat tcccaaatta ttccatcatt
     3481 aaaagatacg aggcgcgtgt aagttacagg caagcgatcc gtcccgagcg gccgcgtgtt
     3541 acaaccaatt aaccaattct gattagaaaa actcatcgag catcaaatga aactgcaatt
     3601 tattcatatc aggattatca ataccatatt tttgaaaaag ccgtttctgt aatgaaggag
     3661 aaaactcacc gaggcagttc cataggatgg caagatcctg gtatcggtct gcgattccga
     3721 ctcgtccaac atcaatacaa cctattaatt tcccctcgtc aaaaataagg ttatcaagtg
     3781 agaaatcacc atgagtgacg actgaatccg gtgagaatgg caaaagctta tgcatttctt
     3841 tccagacttg ttcaacaggc cagccattac gctcgtcatc aaaatcactc gcatcaacca
     3901 aaccgttatt cattcgtgat tgcgcctgag cgaggcgaaa tacgcgatcg ctgttaaaag
     3961 gacaattaca aacaggaatc gaatgcaacc ggcgcaggaa cactgccagc gcatcaacaa
     4021 tattttcacc tgaatcagga tattcttcta atacctggaa tgctgttttc ccggggatcg
     4081 cagtggtgag taaccatgca tcatcaggag tacggataaa atgcttgatg gtcggaagag
     4141 gcataaattc cgtcagccag tttagtctga ccatctcatc tgtaacatca ttggcaacgc
     4201 tacctttgcc atgtttcaga aacaactctg gcgcatcggg cttcccatac aatcgataga
     4261 ttgtcgcacc tgattgcccg acattatcgc gagcccattt atacccatat aaatcagcat
     4321 ccatgttgga atttaatcgc ggcctggagc aagacgtttc ccgttgaata tggctcataa
     4381 caccccttgt attactgttt atgtaagcag acagttttat tgttcatgat gatatatttt
     4441 tatcttgtgc aatgtaacat cagagatttt gagacacaac gtggctttgt tgaataaatc
     4501 gaacttttgc tgagttgaag gatcagtcat gaccaaaatc ccttaacgtg agttttcgtt
     4561 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     4621 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     4681 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     4741 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     4801 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     4861 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     4921 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     4981 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     5041 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     5101 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     5161 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     5221 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
     5281 ggataaccgt gcggccgccc ct
//
