LOCUS       04_pBBR1-kan-Pte        7183 bp ds-DNA     circular SYN 08-DEC-2023
DEFINITION  synthetic circular DNA.
ACCESSION
VERSION     .
KEYWORDS    04 pBBR1-kan-Ptet-plugam-ETh_rhi483.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct.
REFERENCE   1  (bases 1 to 7183)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, November 12, 2021 from SnapGene 3.2.1
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7183
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             1..999
                     /codon_start=1
                     /note="mob"
                     /note="mob;RP4 mob, oriT"
                     /translation="MAAYAIMRCKKLAKMGNVAASLKHAYRERETPNADASRTPENEH
                     WAASSTDEAMGRLRELLPEKRRKDAVLAVEYVMTASPEWWKSASQEQQAAFFEKAHKW
                     LADKYGADRIVTASIHRDETSPHMTAFVVPLTQDGRLSAKEFIGNKAQMTRDQTTFAA
                     AVADLGLQRGIEGSKARHTRIQAFYEALERPPVGHVTISPQAVEPRAYAPQGLAEKLG
                     ISKRVETPEAVADRLTKAVRQGYEPALQAAAGAREMRKKADQAQETARDLRERLKPVL
                     DALGPLNRDMQAKAAAIIKAVGEKLLTEQREVQRQKQAQRQQERGRAHFPEKCHLG"
     misc_feature    1119..1168
                     /note="Pneokan"
                     /note="p.;Pneokan"
     CDS             1257..2051
                     /codon_start=1
                     /note="kanR"
                     /note="kanR;from Tn5 neo"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
                     LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
                     QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     misc_feature    2246..2942
                     /note="06 pBBR1-kan-Ptet-GFP-firefly-Jia"
     CDS             complement(2246..2869)
                     /codon_start=1
                     /note="tetR"
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLG
                     TRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERET
                     PTTDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     misc_feature    2580..2599
                     /note="check-5"
     misc_feature    2904..2950
                     /note="HA"
     CDS             2951..3208
                     /codon_start=1
                     /note="plu2934"
                     /translation="MNPYAVYDEIEEKRLEDEHYREIILEQQGMDAEIIYNKLPELAG
                     IFSIETNKLFGELLTENDEAAELVNSLLYELSLMKVKMEDI"
     misc_feature    2951..3208
                     /gene="'eGFP"" /product=""eGFP"" /SECDrawAs=""Gene"" CDS 
                     complement (3540..3571) /gene=""rhaP"" /product=""rhaP"
                     /gene="rhaR"" /product=""rhaR"" /SECDrawAs=""Gene"" CDS 
                     complement (5866..6660) /gene=""kanamycin"
                     /product="""rhaS"" /SECDrawAs=""Gene"" CDS 
                     4629..5567;kanamycin resistance determinant"
                     /note="08 pBBR1-Rha-pluGam-BAS-kan"
                     /note="08 pBBR1-Rha-pluGam-BAS-kan;LOCUS       pJeM1      
                      7117 bp DNA circular SYN 19-JUN-2008 DEFINITION
                     HindIII/EcoRI, Ligation of inverted Fragment 2 - 4056 into
                     Fragment 2 - 4782 ACCESSION pJeM1 KEYWORDS . SOURCE
                     Unknown. ORGANISM Unknown Unclassified. REFERENCE 1 (bases
                     1 to 7117) AUTHORS Self JOURNAL Unpublished. COMMENT
                     SECID/File created by SciEd Central, Scientific &
                     Educational Software FEATURES Location/Qualifiers CDS
                     complement (6918..799) /gene=""mob"" /SECDrawAs=""Gene""
                     CDS 1793..2455 /gene=""rep"" /product=""Rep"
     misc_feature    2951..3208
                     /note="04 PBBR1-kan-ptet-redr-ETh1_rhi483-Jia.gcc.gcs...."
     misc_feature    2951..2972
                     /note="Primer"
     misc_feature    complement(3188..3208)
                     /note="primer"
     misc_feature    3209..3221
                     /note="Untitled"
     CDS             3222..4028
                     /codon_start=1
                     /note="recT"
                     /note="recT;ERF family protein blast to recT:7/27(26%) 
                     11/27(40%) 0/27(0%) 4/5(80%) 5/5(100%) 0/5(0%) blast to
                     red beta:No significant similarity"
                     /translation="MGAVARHDEQSVIPISDAPMVAMIERIVMDPSIPIDRLEQMMAM
                     KERMEDRARTTAKEDRDFEARKAYFAAMSACQKQLPVVVKSQRNSHTNSNYADLAAIE
                     AQAMPIIHDHGFGVSFQPDGYNDNGELRILWEISHNEGYVRNGVGEIPVDGAGTGGKV
                     NKTGTQAFGSTATYGRRYLLCMLFNISTGDDRDGNKAPDTGGPISDEQTIALREKIEA
                     VEADIERFCSRWKIEALKDLPKGQFKDAMVSLDRFGEQKKQRNAEDRNNG"
     misc_feature    complement(3222..3271)
                     /note="HA"
     misc_feature    complement(3829..3848)
                     /note="04-check-3"
     CDS             4021..4683
                     /codon_start=1
                     /note="exonuclease"
                     /note="exonuclease;red-alpha 65/202(32%) 96/202(47%) 
                     9/202(4%)"
                     /translation="MDNIVQGSPEWLAMRAGKVTASRVADVIAKTKTGVSASRAKYAG
                     ELIAERLTGQPAERFTNGAMAWGTEKEPDARKAYEFYRDTDVAEVAFVPHPTIADSGA
                     SPDGLVDVEGLLEIKCPETHTHIETLLNKAVPSKYVTQMMWQMACTGRKWCDFVSFDP
                     RLPESMQFFCQRVHRDEAVIAELEREVVVFINEVRGKVAELRRLYEPAEADPAASMLM
                     AG"
     misc_feature    4690..4695
                     /note="04 PBBR1-kan-ptet-ETh1_rhi483.gcc"
     misc_feature    4690..4695
                     /note="ApaLI"
                     /label="ApaLI"
                     /note="Geneious type: restriction site"
     CDS             4697..5164
                     /codon_start=1
                     /note="HP"
                     /translation="MGTVNRIVANELDKDILIRLIRARKPPFTTTMTDGKHRTNPQNK
                     LQRKWMTEIADQLGDRTAEEVRGECKLMLGVPILRAENEAFCKAYDEHVKPLSYEQKL
                     AFMMMPLDFPVTRLMTTAQSKQYLDAIHRHYSAQGIYLTNPEDRGRAEMARAS"
     misc_feature    5171..5176
                     /note="04 PBBR1-kan-ptet-ETh1_rhi483.gcc"
     misc_feature    5171..5176
                     /note="ApaLI"
                     /label="ApaLI"
                     /note="Geneious type: restriction site"
     terminator      5184..5221
                     /note="ter"
     misc_feature    5184..5221
                     /note="ter"
     misc_feature    5184..5221
                     /note="04 PBBR1-kan-ptet-ETh1_rhi483.gcc"
     misc_feature    complement(5284..5302)
                     /note="JeMrev"
                     /note="JeMrev;sequencing primer"
     CDS             complement(5528..6202)
                     /codon_start=1
                     /note="rep"
                     /translation="MEISMATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTA
                     AMLLHHLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGT
                     VSAYVVNDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQL
                     PTGPGEEPPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTR
                     VTLPRR"
     gene            6281..7098
                     /note="oriV-RK2"
                     /label="oriV-RK2"
     misc_feature    complement(6281..7098)
                     /note="oriV-RK2"
                     /note="oriV-RK2;pBBR1 oriV broad-host-range origin of 
                     replication Cloning vector pKaKa1, complete sequence
                     GenBank: GU074524.1
                     http://www.ncbi.nlm.nih.gov/nuccore/309274422
                     http://en.wikipedia.org/wiki/RK2_plasmid"
     misc_feature    6281..7098
                     /gene="'eGFP"" /product=""eGFP"" /SECDrawAs=""Gene"" CDS 
                     complement (3540..3571) /gene=""rhaP"" /product=""rhaP"
                     /gene="rhaR"" /product=""rhaR"" /SECDrawAs=""Gene"" CDS 
                     complement (5866..6660) /gene=""kanamycin"
                     /product="""rhaS"" /SECDrawAs=""Gene"" CDS 
                     4629..5567;kanamycin resistance determinant"
                     /note="pRK2-Rha-TEpsy-kan.gcc"
                     /note="pRK2-Rha-TEpsy-kan.gcc;LOCUS       pJeM1           
                      7117 bp DNA circular SYN 19-JUN-2008 DEFINITION
                     HindIII/EcoRI, Ligation of inverted Fragment 2 - 4056 into
                     Fragment 2 - 4782 ACCESSION pJeM1 KEYWORDS . SOURCE
                     Unknown. ORGANISM Unknown Unclassified. REFERENCE 1 (bases
                     1 to 7117) AUTHORS Self JOURNAL Unpublished. COMMENT
                     SECID/File created by SciEd Central, Scientific &
                     Educational Software FEATURES Location/Qualifiers CDS
                     complement (6918..799) /gene=""mob"" /SECDrawAs=""Gene""
                     CDS 1793..2455 /gene=""rep"" /product=""Rep"
ORIGIN      
        1 atggcggcat acgcgatcat gcgatgcaag aagctggcga aaatgggcaa cgtggcggcc
       61 agtctcaagc acgcctaccg cgagcgcgag acgcccaacg ctgacgccag caggacgcca
      121 gagaacgagc actgggcggc cagcagcacc gatgaagcga tgggccgact gcgcgagttg
      181 ctgccagaga agcggcgcaa ggacgctgtg ttggcggtcg agtacgtcat gacggccagc
      241 ccggaatggt ggaagtcggc cagccaagaa cagcaggcgg cgttcttcga gaaggcgcac
      301 aagtggctgg cggacaagta cggggcggat cgcatcgtga cggccagcat ccaccgtgac
      361 gaaaccagcc cgcacatgac cgcgttcgtg gtgccgctga cgcaggacgg caggctgtcg
      421 gccaaggagt tcatcggcaa caaagcgcag atgacccgcg accagaccac gtttgcggcc
      481 gctgtggccg atctagggct gcaacggggc atcgagggca gcaaggcacg tcacacgcgc
      541 attcaggcgt tctacgaggc cctggagcgg ccaccagtgg gccacgtcac catcagcccg
      601 caagcggtcg agccacgcgc ctatgcaccg cagggattgg ccgaaaagct gggaatctca
      661 aagcgcgttg agacgccgga agccgtggcc gaccggctga caaaagcggt tcggcagggg
      721 tatgagcctg ccctacaggc cgccgcagga gcgcgtgaga tgcgcaagaa ggccgatcaa
      781 gcccaagaga cggcccgaga ccttcgggag cgcctgaagc ccgttctgga cgccctgggg
      841 ccgttgaatc gggatatgca ggccaaggcc gccgcgatca tcaaggccgt gggcgaaaag
      901 ctgctgacgg aacagcggga agtccagcgc cagaaacagg cccagcgcca gcaggaacgc
      961 gggcgcgcac atttccccga aaagtgccac ctgggatgaa tgtcagctac tgggctatct
     1021 ggacaaggga aaacgcaagc gcaaagagaa agcaggtagc ttgcagtggg cttacatggc
     1081 gatagctaga ctgggcggtt ttatggacag caagcgaacc ggaattgcca gctggggcgc
     1141 cctctggtaa ggttgggaag ccctgcaaag taaactggat ggctttcttg ccgccaagga
     1201 tctgatggcg caggggatca agatctgatc aagagacagg atgaggatcg tttcgcatga
     1261 ttgaacaaga tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct
     1321 atgactgggc acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc
     1381 aggggcgccc ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcagg
     1441 acgaggcagc gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg
     1501 acgttgtcac tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc
     1561 tcctgtcatc tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc
     1621 ggctgcatac gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg
     1681 agcgagcacg tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc
     1741 atcaggggct cgcgccagcc gaactgttcg ccaggctcaa ggcgcgcatg cccgacggcg
     1801 aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc
     1861 gcttttctgg attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag
     1921 cgttggctac ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg
     1981 tgctttacgg tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg
     2041 agttcttctg atagattata ttactaatta attggggacc ctagaggtcc ccttttttat
     2101 tttaaaaatt ttttcacaaa acggtttaca agcataaata aaatggcgcc acaggtgcgg
     2161 ttgctggcgc ctcgacccaa gtaccgccac ctaacaattc gttcaagccg aataacttcg
     2221 tataatgtat gctatacgaa cggtattaag acccactttc acatttaagt tgtttttcta
     2281 atccgcatat gatcaattca aggccgaata agaaggctgg ctctgcacct tggtgatcaa
     2341 ataattcgat agcttgtcgt aataatggcg gcatactatc agtagtaggt gtttcccttt
     2401 cttctttagc gacttgatgc tcttgatctt ccaatacgca acctaaagta aaatgcccca
     2461 cagcgctgag tgcatataat gcattctcta gtgaaaaacc ttgttggcat aaaaaggcta
     2521 attgattttc gagagtttca tactgttttt ctgtaggccg tgtacctaaa tgtacttttg
     2581 ctccatcgcg atgacttagt aaagcacatc taaaactttt agcgttatta cgtaaaaaat
     2641 cttgccagct ttccccttct aaagggcaaa agtgagtatg gtgcctatct aacatctcaa
     2701 tggctaaggc gtcgagcaaa gcccgcttat tttttacatg ccaatacaat gtaggctgct
     2761 ctacacctag cttctgggcg agtttacggg ttgttaaacc ttcgattccg acctcattaa
     2821 gcagctctaa tgcgctgtta atcactttac ttttatctaa tctagacatc attaattcct
     2881 aatttttgtt gacactctat cattgataga gttattttac cactccctat cagtgataga
     2941 gagaattcat atgaacccat atgcagttta tgatgagatt gaagaaaaga ggttggaaga
     3001 tgagcactac agggaaatta tattagagca acaaggcatg gatgccgaaa ttatttataa
     3061 caagcttcct gagcttgctg gaattttttc aatcgaaact aacaaactct ttggcgaatt
     3121 actcacggag aacgatgaag ccgcagagct cgttaattct cttttgtatg aactaagctt
     3181 aatgaaagta aaaatggagg atatatgaag gaggactagt gatgggcgca gtagcacgtc
     3241 acgacgaaca gagcgttatt cccatttccg acgcgccaat ggtggcgatg atcgaacgca
     3301 tcgttatgga cccgtccatt ccgatcgatc gccttgagca aatgatggcg atgaaagagc
     3361 gcatggaaga ccgcgctcga acgacggcga aggaagaccg ggactttgaa gcccgcaagg
     3421 catacttcgc agctatgtcg gcttgccaga agcagttgcc ggttgtcgtc aagtcccaac
     3481 gcaacagcca caccaattcg aactatgccg acctggctgc gatcgaggca caggccatgc
     3541 cgatcatcca tgaccacggc tttggcgtct cgttccagcc ggacggatac aacgacaacg
     3601 gcgagctgcg catcctttgg gagatttccc acaatgaagg atacgtgcgc aacggcgtcg
     3661 gcgaaatccc cgtagatggg gccgggaccg gcggcaaggt caacaagacc ggaactcagg
     3721 ctttcggaag cactgccacc tacggccgcc ggtacctgct ctgcatgctg ttcaacatca
     3781 gcacaggtga tgatcgggac ggcaacaagg ctcctgacac cggcggcccg atctctgatg
     3841 agcagaccat tgcccttcgc gaaaagatcg aggccgtcga ggctgatatc gagcgcttct
     3901 gcagccggtg gaaaatcgaa gctctgaaag acctccccaa gggccaattc aaggacgcca
     3961 tggtttcgct tgaccggttc ggcgagcaga aaaaacagcg caatgcggag gatcgcaaca
     4021 atggataata tcgttcaggg ctcacccgaa tggctggcca tgcgggctgg caaggtaacc
     4081 gcctcgcgtg tcgccgatgt catcgccaag accaagaccg gcgtgtcggc atcccgagcc
     4141 aagtatgctg gcgagcttat cgccgagcgg ttgaccggac aaccggcgga acgcttcacc
     4201 aacggcgcta tggcatgggg aaccgaaaag gaacccgatg cccgcaaggc ctatgagttc
     4261 tatcgggata ctgatgttgc ggaggtcgcc ttcgttcctc acccgacgat cgccgacagc
     4321 ggcgcatcgc ctgacggctt ggtggacgtc gaaggtctct tggagattaa gtgccccgaa
     4381 acgcacaccc acatcgaaac gctgctcaac aaggccgtgc cgtcaaagta cgtcacccag
     4441 atgatgtggc agatggcatg cacggggcgg aaatggtgcg atttcgtatc cttcgaccct
     4501 cgcctgcccg aatccatgca gttcttttgc cagcgcgttc atcgcgatga agccgtgatc
     4561 gccgagcttg agcgcgaagt ggtcgtgttt atcaacgagg tgcgcggcaa ggtcgccgag
     4621 cttcgccgcc tctatgaacc ggccgaagcg gatcctgcag cttcgatgct gatggctggc
     4681 tgaaggaggg tgcactatgg gcaccgtcaa ccgcatcgta gcaaacgaac tcgacaagga
     4741 tatcctcatc cggctgatcc gggcgaggaa gcccccgttc acgacaacga tgacggatgg
     4801 caagcaccgg acgaacccgc agaacaaact gcagcggaaa tggatgaccg agatcgccga
     4861 ccagctcggc gatcggacag ccgaggaagt gcgcggcgaa tgcaagctca tgcttggcgt
     4921 accgatcctg cgcgccgaga acgaggcatt ctgcaaggcc tatgacgaac acgtcaagcc
     4981 gctgtcctac gagcagaagc tggccttcat gatgatgccg cttgacttcc cagttacccg
     5041 gctaatgacc acggcgcaga gcaagcaata cctggatgcg atccaccggc attactcggc
     5101 gcagggtatc tatctcacca atcccgaaga tcgcggtcgc gccgagatgg cgagggcgtc
     5161 atgaaggagg gtgcactgct agccccagcc cgcctaatga gcgggctttt ttttgaacaa
     5221 agcttaccgg tttattgact accggaagca gtgtgaccgt gtgcttctca aatgcctgag
     5281 gccagtttgc tcaggctctc cccgtggagg taataattga cgatatgatc atttattctg
     5341 cctcccagag cctgataaaa acggtgaatc cgttagcgag gtgccgccgg cttccattca
     5401 ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag
     5461 ggcggcgagg cggctacagc cgatagtctg gaacagcgca cttacgggtt gctgcgcaac
     5521 ccaagtgcta ccggcgcggc agcgtgaccc gtgtcggcgg ctccaacggc tcgccatcgt
     5581 ccagaaaaca cggctcatcg ggcatcggca ggcgctgctg cccgcgccgt tcccattcct
     5641 ccgtttcggt caaggctggc aggtctggtt ccatgcccgg aatgccgggc tggctgggcg
     5701 gctcctcgcc ggggccggtc ggtagttgct gctcgcccgg atacagggtc gggatgcggc
     5761 gcaggtcgcc atgccccaac agcgattcgt cctggtcgtc gtgatcaacc accacggcgg
     5821 cactgaacac cgacaggcgc aactggtcgc ggggctggcc ccacgccacg cggtcattga
     5881 ccacgtaggc cgacacggtg ccggggccgt tgagcttcac gacggagatc cagcgctcgg
     5941 ccaccaagtc cttgactgcg tattggaccg tccgcaaaga acgtccgatg agcttggaaa
     6001 gtgtcttctg gctgaccacc acggcgttct ggtggcccat ctgcgccacg aggtgatgca
     6061 gcagcattgc cgccgtgggt ttcctcgcaa taagcccggc ccacgcctca tgcgctttgc
     6121 gttccgtttg cacccagtga ccgggcttgt tcttggcttg aatgccgatt tctctggact
     6181 gcgtggccat gcttatctcc atgcggtagg gtgccgcacg gttgcggcac catgcgcaat
     6241 cagctgcaac ttttcggcag cgcgacaaca attatgcgtt gcgtaaaagt ggcagtcaat
     6301 tacagatttt ctttaaccta cgcaatgagc tattgcgggg ggtgccgcaa tgagctgttg
     6361 cgtacccccc ttttttaagt tgttgatttt taagtctttc gcatttcgcc ctatatctag
     6421 ttctttggtg cccaaagaag ggcacccctg cggggttccc ccacgccttc ggcgcggctc
     6481 cccctccggc aaaaagtggc ccctccgggg cttgttgatc gactgcgcgg ccttcggcct
     6541 tgcccaaggt ggcgctgccc ccttggaacc cccgcactcg ccgccgtgag gctcgggggg
     6601 caggcgggcg ggcttcgcct tcgactgccc ccactcgcat aggcttgggt cgttccaggc
     6661 gcgtcaaggc caagccgctg cgcggtcgct gcgcgagcct tgacccgcct tccacttggt
     6721 gtccaaccgg caagcgaagc gcgcaggccg caggccggag gcttttcccc agagaaaatt
     6781 aaaaaaattg atggggcaag gccgcaggcc gcgcagttgg agccggtggg tatgtggtcg
     6841 aaggctgggt agccggtggg caatccctgt ggtcaagctc gtgggcaggc gcagcctgtc
     6901 catcagcttg tccagcaggg ttgtccacgg gccgagcgaa gcgagccagc cggtggccgc
     6961 tcgcggccat cgtccacata tccacgggct ggcaagggag cgcagcgacc gcgcagggcg
     7021 aagcccggag agcaagcccg tagggcgccg cagccgccgt aggcggtcac gactttgcga
     7081 agcaaagtct agtgagtata ctcaagcatt gagtggcccg ccggaggcac cgccttgcgc
     7141 tgcccccgtc gagccggttg gacaccaaaa gggaggggca ggc
//
