LOCUS       pTCF48                  6152 bp    DNA     linear       16-SEP-2021
FEATURES             Location/Qualifiers
     CDS             complement(4707..5786)
                     /dnas_title="lacI"
                     /vntifkey="4"
                     /label=lacI
     rep_origin      complement(3273..3273)
                     /dnas_title="pBR322 origin"
                     /vntifkey="33"
                     /label=pBR322 origin
     CDS             complement(1752..2564)
                     /dnas_title="Kan"
                     /vntifkey="4"
                     /label=Kan
     CDS             1201..1656
                     /dnas_title="f1 origin"
                     /vntifkey="4"
                     /label=f1 origin
     CDS             118..957
                     /dnas_title="HLA A1101"
                     /vntifkey="4"
                     /label=HLA A1101
                     /note="HLA A1101 "
     CDS             1033..1050
                     /dnas_title="His Tag"
                     /vntifkey="4"
                     /label=His Tag
     CDS             964..1011
                     /dnas_title="BSP Sequence"
                     /vntifkey="4"
                     /label=BSP Sequence
     promoter        21..37
                     /dnas_title="T7 promoter"
                     /vntifkey="30"
                     /label=T7 promoter
     source          1..6152
                     /dnas_title="pTCF48"
ORIGIN      
        1 agatctcgat cccgcgaaat taatacgact cactataggg gaattgtgag cggataacaa
       61 ttcccctcta gaaataattt tgtttaactt taagaaggag atatacatat ggctagcggt
      121 tctcattcta tgagatattt ctacacctcc gtgtcccggc ccggccgcgg ggagccccgc
      181 ttcatcgccg tgggctacgt ggacgacacg cagttcgtgc ggttcgacag cgacgccgcg
      241 agccagagga tggagccgcg ggcgccgtgg atagagcagg aggggccgga gtattgggac
      301 caggagacac ggaatgtgaa ggcccagtca cagactgacc gagtggacct ggggaccctg
      361 cgcggctact acaaccagag cgaggacggt tctcacacca tccagataat gtatggctgc
      421 gacgtggggc cggacgggcg cttcctccgc gggtaccggc aggacgccta cgacggcaag
      481 gattacatcg ccctgaacga ggacctgcgc tcttggaccg cggcggacat ggcagctcag
      541 atcaccaagc gcaagtggga ggcggcccat gcggcggagc agcagagagc ctacctggag
      601 ggccggtgcg tggagtggct ccgcagatac ctggagaacg ggaaggagac gctgcagcgc
      661 acggaccccc ccaagacaca tatgacccac caccccatct ctgaccatga ggccaccctg
      721 aggtgctggg ccctgggctt ctaccctgcg gagatcacac tgacctggca gcgggatggg
      781 gaggaccaga cccaggacac ggagctcgtg gagaccaggc ctgcagggga tggaaccttc
      841 cagaagtggg cggctgtggt ggtgccttct ggagaggagc agagatacac ctgccatgtg
      901 cagcatgagg gtctgcccaa gcccctcacc ctgagatggg agctgtcttc ccagcccgga
      961 tccctgcatc atattctgga tgcacagaaa atggtgtgga atcatcgtta aaagcttgcg
     1021 gccgcactcg agcaccacca ccaccaccac tgagatccgg ctgctaacaa agcccgaaag
     1081 gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct
     1141 aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggatt ggcgaatggg
     1201 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
     1261 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
     1321 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
     1381 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
     1441 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
     1501 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
     1561 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
     1621 acgcgaattt taacaaaata ttaacgttta caatttcagg tggcactttt cggggaaatg
     1681 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     1741 attaattctt agaaaaactc atcgagcatc aaatgaaact gcaatttatt catatcagga
     1801 ttatcaatac catatttttg aaaaagccgt ttctgtaatg aaggagaaaa ctcaccgagg
     1861 cagttccata ggatggcaag atcctggtat cggtctgcga ttccgactcg tccaacatca
     1921 atacaaccta ttaatttccc ctcgtcaaaa ataaggttat caagtgagaa atcaccatga
     1981 gtgacgactg aatccggtga gaatggcaaa agtttatgca tttctttcca gacttgttca
     2041 acaggccagc cattacgctc gtcatcaaaa tcactcgcat caaccaaacc gttattcatt
     2101 cgtgattgcg cctgagcgag acgaaatacg cgatcgctgt taaaaggaca attacaaaca
     2161 ggaatcgaat gcaaccggcg caggaacact gccagcgcat caacaatatt ttcacctgaa
     2221 tcaggatatt cttctaatac ctggaatgct gttttcccgg ggatcgcagt ggtgagtaac
     2281 catgcatcat caggagtacg gataaaatgc ttgatggtcg gaagaggcat aaattccgtc
     2341 agccagttta gtctgaccat ctcatctgta acatcattgg caacgctacc tttgccatgt
     2401 ttcagaaaca actctggcgc atcgggcttc ccatacaatc gatagattgt cgcacctgat
     2461 tgcccgacat tatcgcgagc ccatttatac ccatataaat cagcatccat gttggaattt
     2521 aatcgcggcc tagagcaaga cgtttcccgt tgaatatggc tcataacacc ccttgtatta
     2581 ctgtttatgt aagcagacag ttttattgtt catgaccaaa atcccttaac gtgagttttc
     2641 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     2701 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     2761 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     2821 accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     2881 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     2941 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     3001 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     3061 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     3121 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     3181 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     3241 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     3301 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
     3361 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
     3421 cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct
     3481 tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct
     3541 gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg
     3601 cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat
     3661 ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt
     3721 catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt
     3781 cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg
     3841 tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg
     3901 cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga
     3961 tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta
     4021 aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc
     4081 gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga
     4141 tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga
     4201 aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc
     4261 acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta
     4321 gccgggtcct caacgacagg agcacgatca tgcgcacccg tggggccgcc atgccggcga
     4381 taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga
     4441 gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa
     4501 agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga
     4561 taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc
     4621 tgactgggtt gaaggctctc aagggcatcg gtcgagatcc cggtgcctaa tgagtgagct
     4681 aacttacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
     4741 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgccagg
     4801 gtggtttttc ttttcaccag tgagacgggc aacagctgat tgcccttcac cgcctggccc
     4861 tgagagagtt gcagcaagcg gtccacgctg gtttgcccca gcaggcgaaa atcctgtttg
     4921 atggtggtta acggcgggat ataacatgag ctgtcttcgg tatcgtcgta tcccactacc
     4981 gagatatccg caccaacgcg cagcccggac tcggtaatgg cgcgcattgc gcccagcgcc
     5041 atctgatcgt tggcaaccag catcgcagtg ggaacgatgc cctcattcag catttgcatg
     5101 gtttgttgaa aaccggacat ggcactccag tcgccttccc gttccgctat cggctgaatt
     5161 tgattgcgag tgagatattt atgccagcca gccagacgca gacgcgccga gacagaactt
     5221 aatgggcccg ctaacagcgc gatttgctgg tgacccaatg cgaccagatg ctccacgccc
     5281 agtcgcgtac cgtcttcatg ggagaaaata atactgttga tgggtgtctg gtcagagaca
     5341 tcaagaaata acgccggaac attagtgcag gcagcttcca cagcaatggc atcctggtca
     5401 tccagcggat agttaatgat cagcccactg acgcgttgcg cgagaagatt gtgcaccgcc
     5461 gctttacagg cttcgacgcc gcttcgttct accatcgaca ccaccacgct ggcacccagt
     5521 tgatcggcgc gagatttaat cgccgcgaca atttgcgacg gcgcgtgcag ggccagactg
     5581 gaggtggcaa cgccaatcag caacgactgt ttgcccgcca gttgttgtgc cacgcggttg
     5641 ggaatgtaat tcagctccgc catcgccgct tccacttttt cccgcgtttt cgcagaaacg
     5701 tggctggcct ggttcaccac gcgggaaacg gtctgataag agacaccggc atactctgcg
     5761 acatcgtata acgttactgg tttcacattc accaccctga attgactctc ttccgggcgc
     5821 tatcatgcca taccgcgaaa ggttttgcgc cattcgatgg tgtccgggat ctcgacgctc
     5881 tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac
     5941 cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg
     6001 gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc
     6061 ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat
     6121 gccggccacg atgcgtccgg cgtagaggat cg
//

