LOCUS       Exported                2078 bp DNA     circular SYN 04-JAN-2022
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2078)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 4, 2022 from SnapGene Viewer 6.0.0
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..2078
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(97..957)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1:  97 .. 888 / #ccffcc
                        2: 889 .. 957 / #ccffcc / signal sequence
                     Cleavage site after base 888"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(958..1062)
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: LEFT"
     rep_origin      1165..1753
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
ORIGIN
        1 aagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
       61 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
      121 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
      181 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcggga
      241 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
      301 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
      361 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
      421 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
      481 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
      541 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
      601 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
      661 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
      721 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
      781 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
      841 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
      901 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
      961 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
     1021 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
     1081 gccacctgtc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     1141 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     1201 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     1261 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
     1321 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     1381 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     1441 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     1501 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     1561 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     1621 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     1681 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     1741 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     1801 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtagtcggtc
     1861 tctgacctgg ttgttgcatg aaagcccgcc gcaaggcggg tttttcgtat tggacccggc
     1921 ccatctattt ttaagcaaaa gcaatctttg gctgggcatg gcaacctttt tcatattaat
     1981 atataataaa ggcatcttat atacttgccc gcgccatgcc gctgaaaaac attgattcag
     2041 atcaatccgc gttgaaattt aaaaatatgg ctgagacc
//
