LOCUS       pBTK622_(tdk/kanR)      3341 bp ds-DNA     circular     16-JUN-2021
DEFINITION  .
ACCESSION   urn.local...1453785029635.125                                      
VERSION     urn.local...1453785029635.125                                      
FEATURES             Location/Qualifiers
     misc_feature    3..8
                     /label="BsaI"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    5..13
                     /label="CGAGACGGAAAGTGAAACGTGATTTCATGCGTCATTTTGAACATTTTGTAAATCTTATTTAATAATGTGTGCGGCAATTCACATTTAATTTATGAATGTTTTCTTAACATCGCGGCAACTCAAGAAACGGCAGGTTCGGATCTTAGCTACTAGAGAAAGAGGAGAAATACTAGATGCGTAAAGGCGAAGAGCTGTTCACTGGTGTCGTCCCTATTCTGGTGGAACTGGATGGTGATGTCAACGGTCATAAGTTTTCCGTGCGTGGCGAGGGTGAAGGTGACGCAACTAATGGTAAACTGACGCTGAAGTTCATCTGTACTACTGGTAAACTGCCGGTTCCTTGGCCGACTCTGGTAACGACGCTGACTTATGGTGTTCAGTGCTTTGCTCGTTATCCGGACCATATGAAGCAGCATGACTTCTTCAAGTCCGCCATGCCGGAAGGCTATGTGCAGGAACGCACGATTTCCTTTAAGGATGACGGCACGTACAAAACGCGTGCGGAAGTGAAATTTGAAGGCGATACCCTGGTAAACCGCATTGAGCTGAAAGGCATTGACTTTAAAGAGGACGGCAATATCCTGGGCCATAAGCTGGAATACAATTTTAACAGCCACAATGTTTACATCACCGCCGATAAACAAAAAAATGGCATTAAAGCGAATTTTAAAATTCGCCACAACGTGGAGGATGGCAGCGTGCAGCTGGCTGATCACTACCAGCAAAACACTCCAATCGGTGATGGTCCTGTTCTGCTGCCAGACAATCACTATCTGAGCACGCAAAGCGTTCTGTCTAAAGATCCGAACGAGAAACGCGATCATATGGTTCTGCTGGAGTTCGTAACCGCAGCGGGCATCACGCATGGTATGGATGAACTGTACAAATGACCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCTACTAGAGTCACACTGGCTCACCTTCGGGTGGGCCTTTCTGCGTTTATACGTCTCT"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    10..13
                     /label="Type 2 connector"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     promoter        14..75
                     /label="tdk promoter region"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     gene            14..1688
                     /label="tdk/kan"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     gene            76..693
                     /label="tdk gene"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    complement(668..689)
                     /label="Repeat Creation 5'"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     promoter        694..858
                     /label="kan promoter region"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    726..731
                     /label="BamHI restriction site"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     primer_bind     726..742
                     /label="FW tm 60deg"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     gene            859..1674
                     /label="kan gene"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    882..991
                     /label="Kan spacer FW"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    960..991
                     /label="Kan spacer"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    complement(960..1069)
                     /label="Kan spacer RV"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    complement(1689..1692)
                     /label="Type4 connector"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    1689..1699
                     /label="Final_RV tdkkan Golden_type4 Copy (reversed)(concatenated sequence 2)"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    complement(1694..1699)
                     /label="BsaI"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    complement(1702..1810)
                     /label="CamR Terminator"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     CDS             complement(1811..2470)
                     /label="CamR"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     promoter        complement(2471..2575)
                     /label="CamR Promoter"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     rep_origin      complement(2576..3339)
                     /label="ColE1"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
ORIGIN
        1 tcggtctcaa acgcccagcc tccaattcaa atcataaaaa atttatttgc tttattaaag
       61 aggagaaatt aattaatggc acagctatat ttctactatt ccgcaatgaa tgcgggtaag
      121 tctacagcat tgttgcaatc ttcatacaat taccaggaac gcggcatgcg cactgtcgta
      181 tatacggcag aaattgatga tcgctttggt gccgggaaag tcagttcgcg tataggtttg
      241 tcatcgcctg caaaattatt taaccaaaat tcatcattat ttgatgagat tcgtgcggaa
      301 catgaacagc aggcaattca ttgcgtactg gttgatgaat gccagttttt aaccagacaa
      361 caagtatatg aattatcgga ggttgtcgat caactcgata tacccgtact ttgttatggt
      421 ttacgtaccg attttcgagg tgaattattt attggcagcc aatacttact ggcatggtcc
      481 gacaaactgg ttgaattaaa aaccatctgt ttttgtggcc gtaaagcaag catggtgctg
      541 cgtcttgatc aagcaggcag accttataac gaaggtgagc aggtggtaat tggtggtaat
      601 gaacgatacg tttctgtatg ccgtaaacac tataaagagg cgttacaagt cgactcatta
      661 acggctattc aggaaaggca tcgccacgat taacggccgc caccgcggtg gagctcggta
      721 cccggggatc ctctagagcg gacccgggaa agccacgttg tgtctcaaaa tctctgatgt
      781 tacattgcac aagataaaaa tatatcatca tgaacaataa aactgtctgc ttacataaac
      841 agtaatacaa ggggtgttat gagccatatt caacgggaaa cgtcttgctc gaggccgcga
      901 ttaaattcca acatggatgc tgatttatat gggtataaat gggctcgcga taatgtcggg
      961 caatcaggtg cgacaatcta tcgattgtat gggaagcccg atgcgccaga gttgtttctg
     1021 aaacatggca aaggtagcgt tgccaatgat gttacagatg agatggtcag actaaactgg
     1081 ctgacggaat ttatgcctct tccgaccatc aagcatttta tccgtactcc tgatgatgca
     1141 tggttactca ccactgcgat ccccgggaaa acagcattcc aggtattaga agaatatcct
     1201 gattcaggtg aaaatattgt tgatgcgctg gcagtgttcc tgcgccggtt gcattcgatt
     1261 cctgtttgta attgtccttt taacagcgat cgcgtatttc gtctcgctca ggcgcaatca
     1321 cgaatgaata acggtttggt tgatgcgagt gattttgatg acgagcgtaa tggctggcct
     1381 gttgaacaag tctggaaaga aatgcataag cttttgccat tctcaccgga ttcagtcgtc
     1441 actcatggtg atttctcact tgataacctt atttttgacg aggggaaatt aataggttgt
     1501 attgatgttg gacgagtcgg aatcgcagac cgataccagg atcttgccat cctatggaac
     1561 tgcctcggtg agttttctcc ttcactacag aaacggcttt ttcaaaaata tggtattgat
     1621 aatcctgata tgaataaatt gcagtttcat ttgatgctcg atgagttttt ctaagcatgc
     1681 ggagctgggc tgtgagacca gaccaataaa aaacgcccgg cggcaaccga gcgttctgaa
     1741 caaatccaga tggagttctg aggtcattac tggatctatc aacaggagtc caagcgagct
     1801 cgatatcaaa ttacgccccg ccctgccact catcgcagta ctgttgtaat tcattaagca
     1861 ttctgccgac atggaagcca tcacaaacgg catgatgaac ctgaatcgcc agcggcatca
     1921 gcaccttgtc gccttgcgta taatatttgc ccatggtgaa aacgggggcg aagaagttgt
     1981 ccatattggc cacgtttaaa tcaaaactgg tgaaactcac ccagggattg gctgaaacga
     2041 aaaacatatt ctcaataaac cctttaggga aataggccag gttttcaccg taacacgcca
     2101 catcttgcga atatatgtgt agaaactgcc ggaaatcgtc gtggtattca ctccagagcg
     2161 atgaaaacgt ttcagtttgc tcatggaaaa cggtgtaaca agggtgaaca ctatcccata
     2221 tcaccagctc accgtctttc attgccatac gaaattccgg atgagcattc atcaggcggg
     2281 caagaatgtg aataaaggcc ggataaaact tgtgcttatt tttctttacg gtctttaaaa
     2341 aggccgtaat atccagctga acggtctggt tataggtaca ttgagcaact gactgaaatg
     2401 cctcaaaatg ttctttacga tgccattggg atatatcaac ggtggtatat ccagtgattt
     2461 ttttctccat tttagcttcc ttagctcctg aaaatctcga taactcaaaa aatacgcccg
     2521 gtagtgatct tatttcatta tggtgaaagt tggaacctct tacgtgcccg atcaatcatg
     2581 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     2641 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     2701 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     2761 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
     2821 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     2881 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     2941 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     3001 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     3061 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     3121 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     3181 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     3241 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     3301 ttctttcctg cgttatcccc tgattctgtg gataaccgta g
//