LOCUS       Exported                8930 bp ds-DNA     circular SYN 22-DEC-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8930)
  TITLE     2-series LIC N-terminal fusion vectors for E. coli expression
REFERENCE   2  (bases 1 to 8930)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 22, 2021 from SnapGene 4.2.11
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8930
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     complement(18..36)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        104..208
                     /gene="bla"
                     /label=AmpR promoter
     CDS             209..1069
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(427..446)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1240..1828
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1729..1748
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1982..1999
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    2014..2156
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(2082..2101)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2242..2264
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(2258..2449)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     3907..3926
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     4010..4029
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4010..4028
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             4117..4139
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             4147..8337
                     /codon_start=1
                     /label=CasX.2 dead with tags
                     /translation="MKSSHHHHHHHHHHGSSMKIEEGKLVIWINGDKGYNGLAEVGKKF
                     EKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQ
                     DKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKS
                     ALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHM
                     NADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLS
                     AGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATME
                     NAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQTNSSSNNNNNNNNNNL
                     GIEENLYFQSNAMQEIKRINKIRRRLVKDSNTKKAGKTGPMKTLLVRVMTPDLRERLEN
                     LRKKPENIPQPISNTSRANLNKLLTDYTEMKKAILHVYWEEFQKDPVGLMSRVAQPAPK
                     NIDQRKLIPVKDGNERLTSSGFACSQCCQPLYVYKLEQVNDKGKPHTNYFGRCNVSEHE
                     RLILLSPHKPEANDELVTYSLGKFGQRALDFYSIHVTRESNHPVKPLEQIGGNSCASGP
                     VGKALSDACMGAVASFLTKYQDIILEHQKVIKKNEKRLANLKDIASANGLAFPKITLPP
                     QPHTKEGIEAYNNVVAQIVIWVNLNLWQKLKIGRDEAKPLQRLKGFPSFPLVERQANEV
                     DWWDMVCNVKKLINEKKEDGKVFWQNLAGYKRQEALLPYLSSEEDHKKREGSLENPKKF
                     ARYQFGDLLLHLEKKHGEDWGKVYDEAWERIDKKVEGLSKHIKLEEERRSEDAQSKAAL
                     TDWLRAKASFVIEGLKEADKDEFCRCELKLQKWYGDLRGKPFAIEAENSILDISGFSKQ
                     YNCAFIWQKDGVKKLNLYLIINYFKGGKLRFKKIKPEAFEANRFYTVINKKSGEIVPME
                     VNFNFDDPNLIILPLAFGKRQGREFIWNDLLSLETGSLKLANGRVIEKTLYNRRTRQDE
                     PALFVALTFERREVLDSSNIKPMNLIGIDRGENIPAVIALTDPEGCPLSRFKDSLGNPT
                     HILRIGESYKEKQRTIQAAKEVEQRRAGGYSRKYASKAKNLADDMVRNTARDLLYYAVT
                     QDAMLIFENLSRGFGRQGKRTFMAERQYTRMEDWLTAKLAYEGLPSKTYLSKTLAQYTS
                     KTCSNCGFTITSADYDRVLEKLKKTATGWMTTINGKELKVEGQITYYNRYKRQNVVKDL
                     SVELDRLSEESVNNDISSWTKGRSGEALSLLKKRFSHRPVQEKFVCLNCGFETHADEQA
                     ALNIARSWLFLRSQEYKKYQTNKTTGNTDKRAFVETWQSFYRKKLKEVWKPAV"
     CDS             4159..4188
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             4198..5298
                     /codon_start=1
                     /gene="malE (mutated)"
                     /product="maltose binding protein from E. coli"
                     /label=MBP
                     /note="This version of the gene does not encode a signal 
                     sequence, so MBP will remain in the cytosol."
                     /translation="MKIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLE
                     EKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKL
                     IAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIA
                     ADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGE
                     TAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFL
                     ENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMSAF
                     WYAVRTAVINAASGRQTVDEALKDAQT"
     primer_bind     5272..5295
                     /label=MBP-F
                     /note="Maltose binding protein, forward primer"
     CDS             5353..5373
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             5371..8337
                     /codon_start=1
                     /label=CasX.2 dead cleaved
                     /translation="SNAMQEIKRINKIRRRLVKDSNTKKAGKTGPMKTLLVRVMTPDLR
                     ERLENLRKKPENIPQPISNTSRANLNKLLTDYTEMKKAILHVYWEEFQKDPVGLMSRVA
                     QPAPKNIDQRKLIPVKDGNERLTSSGFACSQCCQPLYVYKLEQVNDKGKPHTNYFGRCN
                     VSEHERLILLSPHKPEANDELVTYSLGKFGQRALDFYSIHVTRESNHPVKPLEQIGGNS
                     CASGPVGKALSDACMGAVASFLTKYQDIILEHQKVIKKNEKRLANLKDIASANGLAFPK
                     ITLPPQPHTKEGIEAYNNVVAQIVIWVNLNLWQKLKIGRDEAKPLQRLKGFPSFPLVER
                     QANEVDWWDMVCNVKKLINEKKEDGKVFWQNLAGYKRQEALLPYLSSEEDHKKREGSLE
                     NPKKFARYQFGDLLLHLEKKHGEDWGKVYDEAWERIDKKVEGLSKHIKLEEERRSEDAQ
                     SKAALTDWLRAKASFVIEGLKEADKDEFCRCELKLQKWYGDLRGKPFAIEAENSILDIS
                     GFSKQYNCAFIWQKDGVKKLNLYLIINYFKGGKLRFKKIKPEAFEANRFYTVINKKSGE
                     IVPMEVNFNFDDPNLIILPLAFGKRQGREFIWNDLLSLETGSLKLANGRVIEKTLYNRR
                     TRQDEPALFVALTFERREVLDSSNIKPMNLIGIDRGENIPAVIALTDPEGCPLSRFKDS
                     LGNPTHILRIGESYKEKQRTIQAAKEVEQRRAGGYSRKYASKAKNLADDMVRNTARDLL
                     YYAVTQDAMLIFENLSRGFGRQGKRTFMAERQYTRMEDWLTAKLAYEGLPSKTYLSKTL
                     AQYTSKTCSNCGFTITSADYDRVLEKLKKTATGWMTTINGKELKVEGQITYYNRYKRQN
                     VVKDLSVELDRLSEESVNNDISSWTKGRSGEALSLLKKRFSHRPVQEKFVCLNCGFETH
                     ADEQAALNIARSWLFLRSQEYKKYQTNKTTGNTDKRAFVETWQSFYRKKLKEVWKPAV"
     CDS             5380..8337
                     /codon_start=1
                     /label=PlmCasX
                     /translation="MQEIKRINKIRRRLVKDSNTKKAGKTGPMKTLLVRVMTPDLRERL
                     ENLRKKPENIPQPISNTSRANLNKLLTDYTEMKKAILHVYWEEFQKDPVGLMSRVAQPA
                     PKNIDQRKLIPVKDGNERLTSSGFACSQCCQPLYVYKLEQVNDKGKPHTNYFGRCNVSE
                     HERLILLSPHKPEANDELVTYSLGKFGQRALDFYSIHVTRESNHPVKPLEQIGGNSCAS
                     GPVGKALSDACMGAVASFLTKYQDIILEHQKVIKKNEKRLANLKDIASANGLAFPKITL
                     PPQPHTKEGIEAYNNVVAQIVIWVNLNLWQKLKIGRDEAKPLQRLKGFPSFPLVERQAN
                     EVDWWDMVCNVKKLINEKKEDGKVFWQNLAGYKRQEALLPYLSSEEDHKKREGSLENPK
                     KFARYQFGDLLLHLEKKHGEDWGKVYDEAWERIDKKVEGLSKHIKLEEERRSEDAQSKA
                     ALTDWLRAKASFVIEGLKEADKDEFCRCELKLQKWYGDLRGKPFAIEAENSILDISGFS
                     KQYNCAFIWQKDGVKKLNLYLIINYFKGGKLRFKKIKPEAFEANRFYTVINKKSGEIVP
                     MEVNFNFDDPNLIILPLAFGKRQGREFIWNDLLSLETGSLKLANGRVIEKTLYNRRTRQ
                     DEPALFVALTFERREVLDSSNIKPMNLIGIDRGENIPAVIALTDPEGCPLSRFKDSLGN
                     PTHILRIGESYKEKQRTIQAAKEVEQRRAGGYSRKYASKAKNLADDMVRNTARDLLYYA
                     VTQDAMLIFENLSRGFGRQGKRTFMAERQYTRMEDWLTAKLAYEGLPSKTYLSKTLAQY
                     TSKTCSNCGFTITSADYDRVLEKLKKTATGWMTTINGKELKVEGQITYYNRYKRQNVVK
                     DLSVELDRLSEESVNNDISSWTKGRSGEALSLLKKRFSHRPVQEKFVCLNCGFETHADE
                     QAALNIARSWLFLRSQEYKKYQTNKTTGNTDKRAFVETWQSFYRKKLKEVWKPAV"
     primer_bind     5380..5408
                     /label=AmpX.2
     primer_bind     5829..5851
                     /label=bX.2-150sq.F
     primer_bind     6502..6523
                     /label=bX.2-375sq.F
     misc_feature    6541..6573
                     /label=Region 1 from DpbCasX
     misc_feature    6544..6564
                     /label=Region 3 loop from DpbCasX
     primer_bind     7122..7150
                     /label=bX.2-575sq.F
     primer_bind     7352..7401
                     /label=X.2qc#1
     misc_feature    7375..7377
                     /label=Active site
     primer_bind     7643..7688
                     /label=X.2qc#2
     misc_structure  7666..7668
                     /label=Active site
     primer_bind     7723..7745
                     /label=bX.2-775sq.F
     primer_bind     8139..8192
                     /label=X.2qc#3
     misc_feature    8164..8166
                     /label=Active site
     primer_bind     complement(8321..8337)
                     /label=X.2Amp Rev
     primer_bind     complement(8466..8484)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      8480..8527
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(8893..8921)
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
ORIGIN
        1 ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat
       61 aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
      121 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
      181 gcttcaataa cattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
      241 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
      301 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
      361 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
      421 agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg
      481 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
      541 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
      601 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
      661 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
      721 accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact
      781 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
      841 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
      901 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
      961 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     1021 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
     1081 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     1141 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     1201 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     1261 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     1321 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     1381 tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     1441 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     1501 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     1561 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     1621 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     1681 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     1741 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     1801 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     1861 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
     1921 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
     1981 cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca
     2041 tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc
     2101 gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc
     2161 gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt
     2221 acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac
     2281 cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga
     2341 tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc
     2401 ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg
     2461 tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca
     2521 cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac
     2581 tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg
     2641 ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga
     2701 acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga
     2761 agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc
     2821 gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg
     2881 tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga
     2941 tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg
     3001 tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc
     3061 cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg
     3121 acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt
     3181 ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt
     3241 taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg
     3301 cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat
     3361 aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc
     3421 ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt
     3481 gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat
     3541 cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg
     3601 tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg
     3661 cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc
     3721 cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc
     3781 cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc
     3841 tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc
     3901 cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc
     3961 ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaatt aatacgactc
     4021 actataggga gaccacaacg gtttccctct agtgccggct ccggagagct ctttaattaa
     4081 gcggccgccc tgcaggactc gagttctaga aataattttg tttaacttta agaaggagat
     4141 atacatatga aatcttctca ccatcaccat caccatcacc atcaccatgg ttcttctatg
     4201 aaaatcgaag aaggtaaact ggtaatctgg attaacggcg ataaaggcta taacggtctc
     4261 gctgaagtcg gtaagaaatt cgagaaagat accggaatta aagtcaccgt tgagcatccg
     4321 gataaactgg aagagaaatt cccacaggtt gcggcaactg gcgatggccc tgacattatc
     4381 ttctgggcac acgaccgctt tggtggctac gctcaatctg gcctgttggc tgaaatcacc
     4441 ccggacaaag cgttccagga caagctgtat ccgtttacct gggatgccgt acgttacaac
     4501 ggcaagctga ttgcttaccc gatcgctgtt gaagcgttat cgctgattta taacaaagat
     4561 ctgctgccga acccgccaaa aacctgggaa gagatcccgg cgctggataa agaactgaaa
     4621 gcgaaaggta agagcgcgct gatgttcaac ctgcaagaac cgtacttcac ctggccgctg
     4681 attgctgctg acgggggtta tgcgttcaag tatgaaaacg gcaagtacga cattaaagac
     4741 gtgggcgtgg ataacgctgg cgcgaaagcg ggtctgacct tcctggttga cctgattaaa
     4801 aacaaacaca tgaatgcaga caccgattac tccatcgcag aagctgcctt taataaaggc
     4861 gaaacagcga tgaccatcaa cggcccgtgg gcatggtcca acatcgacac cagcaaagtg
     4921 aattatggtg taacggtact gccgaccttc aagggtcaac catccaaacc gttcgttggc
     4981 gtgctgagcg caggtattaa cgccgccagt ccgaacaaag agctggcaaa agagttcctc
     5041 gaaaactatc tgctgactga tgaaggtctg gaagcggtta ataaagacaa accgctgggt
     5101 gccgtagcgc tgaagtctta cgaggaagag ttggcgaaag atccacgtat tgccgccact
     5161 atggaaaacg cccagaaagg tgaaatcatg ccgaacatcc cgcagatgtc cgctttctgg
     5221 tatgccgtgc gtactgcggt gatcaacgcc gccagcggtc gtcagactgt cgatgaagcc
     5281 ctgaaagacg cgcagactaa ttcgagctcg aacaacaaca acaataacaa taacaacaac
     5341 ctcgggatcg aggaaaacct gtacttccaa tccaatgcaa tgcaggaaat aaaaaggata
     5401 aataaaatac gaaggagatt ggtaaaggat agcaacacga aaaaagccgg caaaaccggc
     5461 cctatgaaaa ccttgctcgt tcgggttatg acacctgacc tgagagaaag gttagagaat
     5521 cttcgcaaaa agccggaaaa cattcctcag cccatttcaa atacttcacg tgcaaattta
     5581 aataaactcc tcactgacta tacggaaatg aagaaagcaa tcctgcatgt ttattgggaa
     5641 gagttccaaa aagaccctgt cggattgatg agcagggttg cacaaccagc gcccaagaat
     5701 attgatcaga gaaaattgat tccggtgaag gacggaaatg agagactaac aagttctgga
     5761 tttgcctgtt ctcagtgctg tcaacccctc tatgtttata agcttgaaca agtgaatgac
     5821 aagggtaagc cccatacaaa ttactttggc cgttgtaatg tctccgagca tgaacgtttg
     5881 atattgctct cgccgcataa accggaggca aatgacgagc tagtaacgta ttcgttgggg
     5941 aagttcggtc aaagggcatt ggacttttat tcaatccacg taacaagaga atcgaaccat
     6001 cctgtaaagc cgctagaaca gatcggtggc aatagctgcg caagtggtcc cgttggtaag
     6061 gctttatctg atgcctgtat gggagcagta gccagtttcc ttacaaagta ccaggacatc
     6121 atcctcgaac accaaaaggt tataaaaaaa aacgaaaaga gattggcaaa tctaaaggat
     6181 atagcaagtg caaacgggct tgcatttcct aaaatcactc ttccaccgca accgcataca
     6241 aaagaaggga ttgaagctta taacaatgtt gttgctcaga tagtgatctg ggtaaacctg
     6301 aatctttggc agaaactcaa aattggcagg gatgaggcaa agcccttaca gcggcttaag
     6361 ggttttccgt ccttccctct tgttgaacgc caggcgaatg aggttgattg gtgggatatg
     6421 gtctgtaatg tcaaaaagtt gattaacgaa aagaaagagg acgggaaggt cttctggcaa
     6481 aatcttgctg gatataaaag gcaggaagcc ttgcttccat atctttcgtc tgaagaagac
     6541 cataaaaagc gcgagggcag tttagagaat ccgaaaaagt ttgcgcgtta tcagtttggt
     6601 gaccttttgc ttcaccttga aaagaaacac ggtgaagatt ggggcaaagt ttatgatgag
     6661 gcatgggaaa gaatagataa aaaagttgaa ggtctgagta agcacataaa gttggaggaa
     6721 gaaagaaggt ctgaagatgc tcaatcaaag gctgccctca ctgattggct cagggcaaag
     6781 gcctcttttg ttattgaagg gctcaaagaa gctgataagg atgagttttg caggtgtgag
     6841 ttaaagcttc aaaagtggta tggagatttg agaggaaaac catttgctat agaagcagag
     6901 aacagcattt tagatataag cggattttct aaacagtata attgtgcatt tatatggcag
     6961 aaagacggcg taaagaagtt aaatctttat ttaataataa attacttcaa aggtggtaag
     7021 ctacgcttca aaaaaatcaa gccagaagct tttgaagcaa ataggtttta tacagtaatt
     7081 aataaaaaaa gcggtgagat tgtgcctatg gaggtcaact tcaattttga tgacccgaat
     7141 ttgataattc tgcctttggc ctttggaaaa aggcagggga gggagtttat ctggaacgac
     7201 ctattgagcc ttgagacggg ttcattgaaa ctcgccaatg gcagggttat tgaaaaaacg
     7261 ctctataaca gaaggacgag acaggatgaa ccagcacttt ttgttgccct gacatttgaa
     7321 agaagagagg tgcttgactc atcgaatata aaaccgatga atctgatagg aatagaccgg
     7381 ggagaaaata tcccggcagt catagcatta acagacccgg aaggatgccc cttgtcaaga
     7441 ttcaaagatt cattgggcaa tccaacgcat attttgcgaa taggagaaag ttataaggaa
     7501 aaacaacgga ctattcaggc tgctaaagaa gttgaacaaa ggcgggcagg cggatattcg
     7561 agaaaatatg catcaaaggc gaagaatctg gcggacgata tggtaagaaa tacagctcgt
     7621 gacctcttat attatgctgt tactcaagat gcaatgctca tttttgaaaa tctttcccgc
     7681 ggttttggta gacaaggcaa gaggactttt atggcggaaa ggcagtacac gaggatggaa
     7741 gactggctga ctgcaaagct tgcctatgaa ggtctgccat caaaaaccta tctttcaaag
     7801 actctggcac agtatacctc aaagacatgt tctaattgtg gttttacaat cacaagtgca
     7861 gattatgaca gggtgctcga aaagctcaag aagacggcta ctggatggat gactacaatc
     7921 aatggaaaag agttaaaagt tgaaggacag ataacatact ataaccggta taaaaggcag
     7981 aatgtggtaa aagacctctc tgtagagctg gatagacttt cggaagagtc ggtaaataat
     8041 gatatttcta gttggacaaa aggccgcagt ggtgaagctt tatctctgct aaaaaagaga
     8101 tttagtcaca ggccggtgca ggaaaagttt gtttgcctga actgtggttt tgaaacccat
     8161 gcagacgaac aagcagcact gaatattgca aggtcgtggc tctttctccg ttctcaagaa
     8221 tataagaagt atcaaaccaa taaaacgacc ggaaatactg acaaaagggc atttgttgaa
     8281 acatggcaat ccttttacag aaagaagctc aaagaagtat ggaaaccagc cgtctgataa
     8341 taacattgga agtggataac ggatccgcga tcgcggcgcg ccacctggtg gccggccggt
     8401 accacgcgtg cgcgctgatc cggctgctaa caaagcccga aaggaagctg agttggctgc
     8461 tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg
     8521 ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga
     8581 tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc
     8641 ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta
     8701 gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg
     8761 gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga
     8821 cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg
     8881 ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa
//
