LOCUS       Exported                8472 bp DNA     circular SYN 04-SEP-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8472)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Nov 19, 2021 from SnapGene 5.3.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8472
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    2205..2520
                     /locus_tag="TRE-tight Promoter"
                     /label=TRE-tight Promoter
     misc_feature    2528..2533
                     /locus_tag="BamHI"
                     /label=BamHI
     misc_feature    2540..3247
                     /locus_tag="mCherry"
                     /label=mCherry
     misc_feature    complement(2627..2644)
                     /locus_tag="mCherry-revseq primer"
                     /label=mCherry-revseq primer
     misc_feature    3133..3151
                     /locus_tag="mCherry-F-seq primer"
                     /label=mCherry-F-seq primer
     misc_feature    3248..3253
                     /locus_tag="BamHI(1)"
                     /label=BamHI
     misc_feature    3249..3253
                     /locus_tag="Sequencing confirmed"
                     /label=Sequencing confirmed
     misc_feature    3249..3253
                     /locus_tag="lacZ"
                     /label=lacZ
     misc_feature    3254..3757
                     /locus_tag="pgk Promoter"
                     /label=pgk Promoter
     misc_feature    3778..4377
                     /locus_tag="Puro"
                     /label=Puro
     primer_bind     complement(5946..5966)
                     /locus_tag="M13-rev"
                     /label=M13-rev
     misc_binding    complement(5972..5994)
                     /locus_tag="LacO"
                     /label=LacO
     rep_origin      complement(6334..7016)
                     /direction=LEFT
                     /locus_tag="ColE1 origin"
                     /label=ColE1 origin
     CDS             complement(7114..7773)
                     /locus_tag="AmpR"
                     /label=AmpR
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgatgaggcc ctttcgtctt cactcgagtt tactccctat
     2221 cagtgataga gaacgtatgt cgagtttact ccctatcagt gatagagaac gatgtcgagt
     2281 ttactcccta tcagtgatag agaacgtatg tcgagtttac tccctatcag tgatagagaa
     2341 cgtatgtcga gtttactccc tatcagtgat agagaacgta tgtcgagttt atccctatca
     2401 gtgatagaga acgtatgtcg agtttactcc ctatcagtga tagagaacgt atgtcgaggt
     2461 aggcgtgtac ggtgggaggc ctatataagc agagctcgtt tagtgaaccg tcagatcgcc
     2521 tggagaagga tccgccacca tggtgagcaa gggcgaggag gataacatgg ccatcatcaa
     2581 ggagttcatg cgcttcaagg tgcacatgga gggctccgtg aacggccacg agttcgagat
     2641 cgagggcgag ggcgagggcc gcccctacga gggcacccag accgccaagc tgaaggtgac
     2701 caagggtggc cccctgccct tcgcctggga catcctgtcc cctcagttca tgtacggctc
     2761 caaggcctac gtgaagcacc ccgccgacat ccccgactac ttgaagctgt ccttccccga
     2821 gggcttcaag tgggagcgcg tgatgaactt cgaggacggc ggcgtggtga ccgtgaccca
     2881 ggactcctcc ctgcaggacg gcgagttcat ctacaaggtg aagctgcgcg gcaccaactt
     2941 cccctccgac ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct cctccgagcg
     3001 gatgtacccc gaggacggcg ccctgaaggg cgagatcaag cagaggctga agctgaagga
     3061 cggcggccac tacgacgctg aggtcaagac cacctacaag gccaagaagc ccgtgcagct
     3121 gcccggcgcc tacaacgtca acatcaagtt ggacatcacc tcccacaacg aggactacac
     3181 catcgtggaa cagtacgaac gcgccgaggg ccgccactcc accggcggca tggacgagct
     3241 gtacaaggga tcctaccggg taggggaggc gcttttccca aggcagtctg gagcatgcgc
     3301 tttagcagcc ccgctgggca cttggcgcta cacaagtggc ctctggcctc gcacacattc
     3361 cacatccacc ggtaggcgcc aaccggctcc gttctttggt ggccccttcg cgccaccttc
     3421 tactcctccc ctagtcagga agttcccccc cgccccgcag ctcgcgtcgt gcaggacgtg
     3481 acaaatggaa gtagcacgtc tcactagtct cgtgcagatg gacagcaccg ctgagcaatg
     3541 gaagcgggta ggcctttggg gcagcggcca atagcagctt tgctccttcg ctttctgggc
     3601 tcagaggctg ggaaggggtg ggtccggggg cgggctcagg ggcgggctca ggggcggggc
     3661 gggcgcccga aggtcctccg gaggcccggc attctgcacg cttcaaaagc gcacgtctgc
     3721 cgcgctgttc tcctcttcct catctccggg cctttcgacc tgcagcccaa gcttaccatg
     3781 accgagtaca agcccacggt gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc
     3841 accctcgccg ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga tccggaccgc
     3901 cacatcgagc gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc
     3961 ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac gccggagagc
     4021 gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt gagcggttcc
     4081 cggctggccg cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc caaggagccc
     4141 gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc agggcaaggg tctgggcagc
     4201 gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag
     4261 acctccgcgc cccgcaacct ccccttctac gagcggctcg gcttcaccgt caccgccgac
     4321 gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg tgcctgacgg
     4381 gcgcgtctgg aacaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct
     4441 taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc
     4501 tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct
     4561 ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga
     4621 cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc
     4681 tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac
     4741 aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaagc tgacgtcctt
     4801 tccatggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt
     4861 cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc
     4921 tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc
     4981 gcctggaatt aattctgcag tcgagaccta gaaaaacatg gagcaatcac aagtagcaat
     5041 acagcagcta ccaatgctga ttgtgcctgg ctagaagcac aagaggagga ggaggtgggt
     5101 tttccagtca cacctcaggt acctttaaga ccaatgactt acaaggcagc tgtagatctt
     5161 agccactttt taaaagaaaa gaggggactg gaagggctaa ttcactccca acgaagacaa
     5221 gatatccttg atctgtggat ctaccacaca caaggctact tccctgatta gcagaactac
     5281 acaccagggc caggggtcag atatccactg acctttggat ggtgctacaa gctagtacca
     5341 gttgagccag ataaggtaga agaggccaat aaaggagaga acaccagctt gttacaccct
     5401 gtgagcctgc atgggatgga tgacccggag agagaagtgt tagagtggag gtttgacagc
     5461 cgcctagcat ttcatcacgt ggcccgagag ctgcatccgg agtacttcaa gaactgctga
     5521 tatcgagctt gctacaaggg actttccgct ggggactttc cagggaggcg tggcctgggc
     5581 gggactgggg agtggcgagc cctcagatcc tgcatataag cagctgcttt ttgcctgtac
     5641 tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc
     5701 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt
     5761 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag
     5821 cagtagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga aatgaatatc
     5881 agagagtgag aggccttgac attgctagcg tttaccgtcg acctctagct agagcttggc
     5941 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
     6001 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
     6061 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
     6121 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
     6181 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     6241 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     6301 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     6361 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     6421 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     6481 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     6541 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     6601 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     6661 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     6721 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     6781 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     6841 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     6901 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     6961 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     7021 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     7081 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
     7141 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
     7201 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
     7261 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
     7321 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
     7381 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
     7441 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
     7501 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
     7561 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
     7621 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
     7681 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
     7741 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
     7801 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
     7861 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
     7921 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
     7981 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
     8041 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
     8101 tgacgtcgac ggatcgggag atcaacttgt ttattgcagc ttataatggt tacaaataaa
     8161 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
     8221 tgtccaaact catcaatgta tcttatcatg tctggatcaa ctggataact caagctaacc
     8281 aaaatcatcc caaacttccc accccatacc ctattaccac tgccaattac ctgtggtttc
     8341 atttactcta aacctgtgat tcctctgaat tattttcatt ttaaagaaat tgtatttgtt
     8401 aaatatgtac tacaaactta gtagttttta aagaaattgt atttgttaaa tatgtactac
     8461 aaacttagta gt
//
