LOCUS       pMYT079                 5632 bp ds-DNA     circular     22-DEC-2021
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    9..531
                     /label="Int.5 5' HA"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    532..551
                     /label="Int.5 5' Barcode"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    598..617
                     /label="ConS FP"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    620..762
                     /label="ConS"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     promoter        771..900
                     /label="BBa_J72163 GlpT Promoter"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     RBS             901..936
                     /label="RBS"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     CDS             937..1635
                     /label="mScarlet"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     terminator      1636..1764
                     /label="BBa_B0015 Terminator"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     promoter        1765..2238
                     /label="SbTDH3 Promoter"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     CDS             2239..2937
                     /label="mScarlet"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     terminator      2938..3086
                     /label="ScRPL15A Terminator"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    3095..3237
                     /label="ConE"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    complement(3240..3259)
                     /label="ConE RP"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    3338..3357
                     /label="Int.5 3' Barcode"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    complement(3358..3869)
                     /label="Int.5 3' HA"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     terminator      complement(3878..3904)
                     /label="KanR Terminator"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     CDS             complement(3905..4720)
                     /label="KanR"
                     /ApEinfo_revcolor=#008000
                     /ApEinfo_fwdcolor=#008000
     promoter        complement(4721..4868)
                     /label="KanR Promoter"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     rep_origin      complement(4869..5632)
                     /label="ColE1"
                     /ApEinfo_revcolor=#7f7f7f
                     /ApEinfo_fwdcolor=#7f7f7f
ORIGIN
        1 GCGGCCGCGT TTCTCCTCCT CTCTTTTTTT CCTCGAACCG GAGTGGAAGA TCCAATAACG
       61 TAATATTACT GATGTTGTTA TTAAAGCTGG CAAAAATAAC ATGAGGCGTA AAACCGCACT
      121 GCGGTAAGAT GAGGGTATAA GGTGGAGATC AGGCGAACAA GCTGTTCTAA ATCATACATA
      181 TGTACAATGA GAACGTGTAA CGATCCAATG AGCGTTTCAT GATGCCATTG TTTAATCAGA
      241 GTGATGAAAA AGAAATATTT GCGACCTTTT TTCGTTACAT TGATCGTGAA ATTTTAATCA
      301 AAGATAATAT AAGGACGTGA GATATTTATC TTTTTACTTG AAATTAACAA TAGAATTGCG
      361 CTAAGCGGAA TAAGAGCTTT CGTAAACCTT TCTATTTGCA CCATTGCGTC AACGTATAAA
      421 ATGGTATGAC CTTTACACAA ACGCATGCTT ATAATCTTAT GTTTTTCATA GGGTGTAATT
      481 TGGTTGATGA CGTAGTCTAA ATTTGATGCT ATCTGCAATT GAGGTACATA TCCTATCAGA
      541 AGTGGGCATC TGAATTCGCT ATCTAGAGTA CCTGATGAGA CGGTCAAACG TGAGACCCTG
      601 TGGTCAAAAG TGCGTTGGCT GGTAGAGCCA CAAACAGCCG GTACAAGCAA CGATCTCCAG
      661 GACCATCTGA ATCATGCGCG GATGACACGA ACTCACGACG GCGATCACAG ACATTAACCC
      721 ACAGTACAGA CACTGCGACA ACGTGGCAAT TCGTCGCAAT ACGCGGCCGC GAAAGTGAAA
      781 CGTGATTTCA TGCGTCATTT TGAACATTTT GTAAATCTTA TTTAATAATG TGTGCGGCAA
      841 TTCACATTTA ATTTATGAAT GTTTTCTTAA CATCGCGGCA ACTCAAGAAA CGGCAGGTTC
      901 GGATCTTAGC TACTAGAGAA AGAGGAGAAA TACTAGATGG TTAGCAAAGG CGAGGCGGTT
      961 ATCAAGGAGT TTATGCGTTT TAAGGTTCAC ATGGAGGGTA GCATGAACGG TCACGAGTTC
     1021 GAGATCGAGG GTGAAGGCGA GGGTCGTCCG TACGAAGGCA CCCAGACCGC GAAGCTGAAA
     1081 GTGACCAAGG GTGGCCCGCT GCCGTTCAGC TGGGACATCC TGAGCCCGCA GTTCATGTAT
     1141 GGCAGCCGTG CGTTTACCAA ACACCCGGCG GACATTCCGG ATTACTATAA GCAAAGCTTC
     1201 CCGGAAGGTT TTAAATGGGA GCGTGTTATG AACTTCGAAG ATGGTGGCGC GGTGACCGTT
     1261 ACCCAGGACA CCAGCCTGGA GGATGGCACC CTGATTTACA AGGTGAAACT GCGTGGCACC
     1321 AACTTTCCGC CGGATGGTCC GGTTATGCAG AAGAAAACGA TGGGTTGGGA AGCGAGCACC
     1381 GAGCGTCTGT ATCCGGAAGA TGGCGTGCTG AAGGGTGATA TCAAAATGGC GCTGCGTCTG
     1441 AAGGACGGTG GCCGTTACCT GGCGGATTTT AAGACCACCT ATAAAGCGAA GAAACCGGTG
     1501 CAAATGCCGG GTGCGTACAA CGTTGACCGT AAACTGGATA TTACCAGCCA CAACGAGGAT
     1561 TATACCGTGG TTGAGCAATA TGAGCGTAGC GAGGGTCGCC ACAGCACCGG CGGCATGGAC
     1621 GAACTGTATA AGTGACCAGG CATCAAATAA AACGAAAGGC TCAGTCGAAA GACTGGGCCT
     1681 TTCGTTTTAT CTGTTGTTTG TCGGTGAACG CTCTCTACTA GAGTCACACT GGCTCACCTT
     1741 CGGGTGGGCC TTTCTGCGTT TATACATTCA TCTTTCACCT GCCATTAGTA ACCCGACTTC
     1801 TCATTGAGCG GGTTACGGCA GCCACAGGCC ACATTCCGAA TGTCTGGGTG AGCGGTCCCT
     1861 TTTCCAGCAT CCACTAAATA TCTCGGATCG CGCTTTTTAA TCTGGCTTCC TGAAAAAAAT
     1921 CAATGGAGTG ATGCAAACTG ACTGGAGCAA AAAGCTGACA CAAGGCAATC GACCTACGTG
     1981 TCTGTCTATT TTCTCACACC TTCTATTACC TTCTAACTCT CTGGGTTGGA AAAAACTGAA
     2041 AAAAAGGTTG TCTCCAGTTT CCACAAATCA TCCCCCTGTT TGATTAATAA ATATATAAAG
     2101 ACGACAACTA TCGATCATAA ACTCATAAAA CTATAACTCC TTTACACTTC TTATTTTATA
     2161 GTTATTCTAT TTTAATTCTT ATTGATTTTA AAACCCCAAG AACTTAGTTT CGAAAACACA
     2221 CACACACAAA CAATTAAAAT GGTGTCTAAA GGTGAAGCCG TCATCAAAGA ATTTATGAGA
     2281 TTCAAGGTTC ACATGGAAGG TTCTATGAAC GGTCACGAAT TTGAAATTGA GGGTGAAGGT
     2341 GAGGGTAGAC CATACGAAGG TACACAAACT GCTAAATTGA AGGTTACAAA AGGTGGTCCT
     2401 TTGCCATTCT CTTGGGATAT TTTGTCTCCA CAATTTATGT ACGGTTCAAG AGCTTTTACA
     2461 AAACACCCAG CTGATATCCC AGATTATTAT AAGCAATCAT TTCCAGAGGG TTTCAAGTGG
     2521 GAAAGAGTCA TGAATTTTGA AGATGGTGGT GCCGTTACTG TTACTCAAGA TACTTCATTG
     2581 GAAGATGGCA CCTTGATCTA CAAGGTTAAG TTAAGAGGTA CTAATTTCCC ACCAGATGGT
     2641 CCTGTTATGC AAAAAAAGAC TATGGGATGG GAAGCTTCTA CTGAAAGATT ATATCCAGAG
     2701 GATGGTGTTT TGAAAGGTGA TATTAAGATG GCCTTGAGAT TGAAAGACGG TGGTAGATAC
     2761 TTGGCTGATT TTAAGACTAC TTACAAGGCC AAAAAGCCAG TTCAAATGCC TGGTGCTTAT
     2821 AACGTTGATA GAAAATTGGA TATCACCTCT CACAACGAAG ATTACACCGT TGTTGAACAG
     2881 TACGAAAGAT CAGAAGGTAG ACATTCTACT GGTGGTATGG ATGAGTTGTA CAAGTAGCTG
     2941 GTTGATGGAA AATATAATTT TATTGGGCAA ACTTTTGTTT ATCTGATGTG TTTTATACTA
     3001 TTATCTTTTT AATTAATGAT TCTATATACA AACCTGTATA TTTTTTCTTT AACCAATTTT
     3061 TTTTTTTATA GACCTAGAGC TGTACTGCGG CCGCGAAATC TGCTCGTCAG TGGTGCTCAC
     3121 ACTGACGAAT CATGTACAGA TCATACCGAT GACTGCCTGG CGACTCACAA CTAAGCAAGA
     3181 CAGCCGGAAC CAGCGCCGGC GAACACCACT GCATATATGG CATATCACAA CAGTCCAGCG
     3241 GGTATTTGGT GCTCGTCTGG GTCTCAGCTG TACGCGTCTC AAGCATAGCA CTAGTTCAGC
     3301 TGCAGCGTAT ACAATGTCTT CCTAGGAAGA CATGAGTGAT TCATTCCTCT GTCTGCGAAG
     3361 AGGTCAATTT CGGGACCAAC CCTTTTAATC GAAAAAAACG TAATTCACTA GGGCAAGGGA
     3421 GAACTTAGCA GCTAATATCG TAAACCTTTC ATACTAAAAA AATGCACTTA CCATCAACAA
     3481 AAAACTCAGG ACCAATTTCC AAGCTTTTCT AGGTGATTGC CTATAACACA AAAAGATTCG
     3541 CTCATACATG AGATTTTTAC ATGTAATAGC AATTTGTTCC GATCAGTTGA AGGTCATCAA
     3601 CGCACGGCAG GTACATCCAC ACCTATCACA AAGCCCTTCA ATAATTCACC TACGTAAAGT
     3661 TATACCGAAA CATGCAAAAT CCATGAAAAA TTCTGTATGA TAACGATCAT ATCCTTTTGT
     3721 ATTGGTGGTA CGATGCTCAA AGATAGTTAT TGTTGCACCT GAGGCAAAAG CGGAAATGAA
     3781 AAATCCAGAT GGGGCCAAAA GCAGAAGTAT TGTGTACAAC AATTGCTTCA GCAGTTTACC
     3841 AAACCGTTTC CCAGCAATCA TCAAAAGTTG CGGCCGCGTG TTACAACCAA TTAACCAATT
     3901 CTGATTAGAA AAACTCATCG AGCATCAAAT GAAACTGCAA TTTATTCATA TCAGGATTAT
     3961 CAATACCATA TTTTTGAAAA AGCCGTTTCT GTAATGAAGG AGAAAACTCA CCGAGGCAGT
     4021 TCCATAGGAT GGCAAGATCC TGGTATCGGT CTGCGATTCC GACTCGTCCA ACATCAATAC
     4081 AACCTATTAA TTTCCCCTCG TCAAAAATAA GGTTATCAAG TGAGAAATCA CCATGAGTGA
     4141 CGACTGAATC CGGTGAGAAT GGCAAAAGCT TATGCATTTC TTTCCAGACT TGTTCAACAG
     4201 GCCAGCCATT ACGCTCGTCA TCAAAATCAC TCGCATCAAC CAAACCGTTA TTCATTCGTG
     4261 ATTGCGCCTG AGCGAGGCGA AATACGCGAT CGCTGTTAAA AGGACAATTA CAAACAGGAA
     4321 TCGAATGCAA CCGGCGCAGG AACACTGCCA GCGCATCAAC AATATTTTCA CCTGAATCAG
     4381 GATATTCTTC TAATACCTGG AATGCTGTTT TCCCGGGGAT CGCAGTGGTG AGTAACCATG
     4441 CATCATCAGG AGTACGGATA AAATGCTTGA TGGTCGGAAG AGGCATAAAT TCCGTCAGCC
     4501 AGTTTAGTCT GACCATCTCA TCTGTAACAT CATTGGCAAC GCTACCTTTG CCATGTTTCA
     4561 GAAACAACTC TGGCGCATCG GGCTTCCCAT ACAATCGATA GATTGTCGCA CCTGATTGCC
     4621 CGACATTATC GCGAGCCCAT TTATACCCAT ATAAATCAGC ATCCATGTTG GAATTTAATC
     4681 GCGGCCTGGA GCAAGACGTT TCCCGTTGAA TATGGCTCAT AACACCCCTT GTATTACTGT
     4741 TTATGTAAGC AGACAGTTTT ATTGTTCATG ATGATATATT TTTATCTTGT GCAATGTAAC
     4801 ATCAGAGATT TTGAGACACA ACGTGGCTTT GTTGAATAAA TCGAACTTTT GCTGAGTTGA
     4861 AGGATCAGTC ATGACCAAAA TCCCTTAACG TGAGTTTTCG TTCCACTGAG CGTCAGACCC
     4921 CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT
     4981 GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC
     5041 TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG TTCTTCTAGT
     5101 GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT
     5161 GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA
     5221 CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC
     5281 ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCTATG
     5341 AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT
     5401 CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC
     5461 TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG
     5521 GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT TTTGCTGGCC
     5581 TTTTGCTCAC ATGTTCTTTC CTGCGTTATC CCCTGATTCT GTGGATAACC GT
//