LOCUS       Exported                6737 bp DNA     linear   SYN 03-JAN-2022
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6737)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 3, 2022 from SnapGene 6.0.0
            https://www.snapgene.com
COMMENT     LOCUS       L297_pMIG_II_PHR        5813 bp ds-DNA     linear       
            08-JUL-2018
            DEFINITION  .
            ACCESSION   
            VERSION     
            SOURCE      .
              ORGANISM  .
            COMMENT     
            COMMENT     
            COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     source          1..6737
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..516
                     /label=5' MMSV LTR
     misc_feature    991..1398
                     /label=gag
     misc_feature    1334..1356
                     /label=MSCV primer
     misc_feature    1418..1462
                     /label=pMIG II-MluI-mBTLA F
                     /note="pMIG II-MluI-mBTLA F"
     misc_feature    1436..1441
                     /label=MluI
     misc_feature    1442..2359
                     /label=mBTLA
                     /note="mBTLA"
     misc_feature    1661..1663
                     /label=N74H
                     /note="N74H"
     misc_feature    1907..1912
                     /label=only in isoform3
                     /note="only in isoform3"
     misc_feature    1961..1963
                     /label=K173E
                     /note="K173E"
     misc_feature    2339..2384
                     /label=pMIG II-MluI-mBTLA R
                     /note="pMIG II-MluI-mBTLA R"
     misc_feature    2360..2365
                     /label=MluI
     misc_feature    2402..2419
                     /label=EH632, rev sequencing primer
     CDS             2420..3136
                     /label=4
     misc_feature    5006..5866
                     /label=AmpR
ORIGIN
        1 tgaaagaccc cacctgtagg tttggcaagc tagcttaagt aacgccattt tgcaaggcat
       61 ggaaaataca taactgagaa tagagaagtt cagatcaagg ttaggaacag agagacagca
      121 gaatatgggc caaacaggat atctgtggta agcagttcct gccccggctc agggccaaga
      181 acagatggtc cccagatgcg gtcccgccct cagcagtttc tagagaacca tcagatgttt
      241 ccagggtgcc ccaaggacct gaaaatgacc ctgtgcctta tttgaactaa ccaatcagtt
      301 cgcttctcgc ttctgttcgc gcgcttctgc tccccgagct caataaaaga gcccacaacc
      361 cctcactcgg cgcgccagtc ctccgataga ctgcgtcgcc cgggtacccg tattcccaat
      421 aaagcctctt gctgtttgca tccgaatcgt ggactcgctg atccttggga gggtctcctc
      481 agattgattg actgcccacc tcgggggtct ttcatttgga ggttccaccg agatttggag
      541 acccctgcct agggaccacc gacccccccg ccgggaggta agctggccag cggtcgtttc
      601 gtgtctgtct ctgtctttgt gcgtgtttgt gccggcatct aatgtttgcg cctgcgtctg
      661 tactagttag ctaactagct ctgtatctgg cggacccgtg gtggaactga cgagttctga
      721 acacccggcc gcaaccctgg gagacgtccc agggactttg ggggccgttt ttgtggcccg
      781 acctgaggaa gggagtcgat gtggaatccg accccgtcag gatatgtggt tctggtagga
      841 gacgagaacc taaaacagtt cccgcctccg tctgaatttt tgctttcggt ttggaaccga
      901 agccgcgcgt cttgtctgct gcagcgctgc agcatcgttc tgtgttgtct ctgtctgact
      961 gtgtttctgt atttgtctga aaattagggc cagactgtta ccactccctt aagtttgacc
     1021 ttaggtcact ggaaagatgt cgagcggatc gctcacaacc agtcggtaga tgtcaagaag
     1081 agacgttggg ttaccttctg ctctgcagaa tggccaacct ttaacgtcgg atggccgcga
     1141 gacggcacct ttaaccgaga cctcatcacc caggttaaga tcaaggtctt ttcacctggc
     1201 ccgcatggac acccagacca ggtcccctac atcgtgacct gggaagcctt ggcttttgac
     1261 ccccctccct gggtcaagcc ctttgtacac cctaagcctc cgcctcctct tcctccatcc
     1321 gccccgtctc tcccccttga acctcctcgt tcgaccccgc ctcgatcctc cctttatcca
     1381 gccctcactc cttctctagg cgccggaatt cgatctggag ctctcgagaa ttctcacgcg
     1441 tatgaagaca gtgcctgcca tgcttgggac tcctcggtta tttagggaat tcttcatcct
     1501 ccatctgggc ctctggagca tcctttgtga gaaagctact aagaggaatg atgaagagtg
     1561 tccagtgcaa cttactatta cgaggaattc caaacagtct gccaggacag gagagttatt
     1621 taaaattcaa tgtcctgtga aatactgtgt tcatagacct aatgtgactt ggtgtaagca
     1681 caatggaaca atctgtgtac cccttgaggt tagccctcag ctatacacta gttgggaaga
     1741 aaatcaatca gttccggttt ttgttctcca ctttaaacca atacatctca gtgataatgg
     1801 gtcgtatagc tgttctacaa acttcaattc tcaagttatt aatagccatt cagtaaccat
     1861 ccatgtgaca gaaaggactc aaaactcttc agaacaccca ctaataacag tatctgacat
     1921 cccagatgcc accaatgcct caggaccatc caccatggaa gagaggccag gcaggacttg
     1981 gctgctttac accttgcttc ctttgggggc attgcttctg ctccttgcct gtgtctgcct
     2041 gctctgcttt ctgaaaagga tccaagggaa agaaaagaag ccttctgact tggcaggaag
     2101 ggacactaac ctggttgata ttccagccag ttccaggaca aatcaccaag cactgccatc
     2161 aggaactgga atttatgata atgatccctg gtctagcatg caggatgaat ctgaattgac
     2221 aattagcttg caatcagaga gaaacaacca gggcattgtt tatgcttctt tgaaccattg
     2281 tgttattgga aggaatccaa gacaggaaaa caacatgcag gaggcaccca cagaatatgc
     2341 atccatttgt gtgagaagta cgcgtctgca ggatatcaag cttgcggtac cgcgggcccg
     2401 ggatccaccg gtcgccacca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc
     2461 catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg
     2521 cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct
     2581 gcccgtgccc tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg
     2641 ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt
     2701 ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa
     2761 gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga
     2821 cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg tctatatcat
     2881 ggccgacaag cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga
     2941 cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt
     3001 gctgctgccc gacaaccact acctgagcac ccagtccaag ctgagcaaag accccaacga
     3061 gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat
     3121 ggacgagctg tacaagtaaa gcggccgcga ctctagagtc gcgactctag agtcgacctg
     3181 caggcatgca agcttcaggt agccggctaa cgttaacaac cggtacctct agaactatag
     3241 ctagcatgcg caaatttaaa gcgctgatat cgataaaata aaagatttta tttagtctcc
     3301 agaaaaaggg gggaatgaaa gaccccacct gtaggtttgg caagctagct taagtaacgc
     3361 cattttgcaa ggcatggaaa atacataact gagaatagag aagttcagat caaggttagg
     3421 aacagagaga cagcagaata tgggccaaac aggatatctg tggtaagcag ttcctgcccc
     3481 ggctcagggc caagaacaga tggtccccag atgcggtccc gccctcagca gtttctagag
     3541 aaccatcaga tgtttccagg gtgccccaag gacctgaaaa tgaccctgtg ccttatttga
     3601 actaaccaat cagttcgctt ctcgcttctg ttcgcgcgct tctgctcccc gagctcaata
     3661 aaagagccca caacccctca ctcggcgcgc cagtcctccg atagactgcg tcgcccgggt
     3721 acccgtgtat ccaataaacc ctcttgcagt tgcatccgac ttgtggtctc gctgttcctt
     3781 gggagggtct cctctgagtg attgactacc cgtcagcggg ggtctttcag aattggtaat
     3841 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
     3901 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa
     3961 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
     4021 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
     4081 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     4141 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     4201 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     4261 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     4321 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     4381 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     4441 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     4501 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     4561 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     4621 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     4681 ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     4741 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     4801 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     4861 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     4921 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     4981 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     5041 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     5101 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
     5161 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     5221 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     5281 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
     5341 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     5401 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
     5461 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
     5521 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
     5581 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
     5641 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
     5701 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
     5761 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
     5821 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
     5881 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
     5941 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg
     6001 tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct
     6061 ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga
     6121 cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag
     6181 cgggtgttgg cgggtgtcgg ggctggctta actatgcggc atcagagcag attgtactga
     6241 gagtgcacca tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca
     6301 ggcgccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt
     6361 cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc
     6421 cagggttttc ccagtcacga cgttgtaaaa cgacggccag tgccacgctc tcccttatgc
     6481 gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca
     6541 aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc
     6601 atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg
     6661 gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg
     6721 atgcgtccgg cgtagag
//
