LOCUS       Exported               10592 bp DNA     circular SYN 03-JAN-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10592)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 3, 2022 from SnapGene 6.0.0
            https://www.snapgene.com
COMMENT     LOCUS        Lxxx_pHR_PD1_mGFP       10592 bp ds-DNA   circular     
            04-JAN-2014
            SOURCE      
              ORGANISM  
            COMMENT     
            COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     source          1..10592
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             34..342
                     /label=3'-LTR
     primer_bind     complement(367..384)
                     /label=SP6
     misc_feature    612..3084
                     /label=pBR322
     CDS             1278..2138
                     /label=bla
     CDS             1476..2135
                     /label=AmpR
     rep_origin      2233..2915
                     /direction=RIGHT
                     /label=ColE1 origin
     promoter        3192..3447
                     /label=SV40 Pr/Enh
     promoter        3276..3472
                     /label=SV40 early promoter
     CDS             3672..4129
                     /label=gpt
     promoter        4531..5380
                     /label=SV40 polyA+Intron
     polyA_signal    complement(5185..5376)
                     /label=SV40 late polyA
     LTR             5549..6233
                     /label=5'-LTR
     misc_feature    6234..6371
                     /label=Psi
     CDS             6372..7556
                     /label=HIV genes
     misc_feature    7570..7747
                     /label=cPPT
     primer_bind     7722..7745
                     /label=cPPT_5'seq
     promoter        7779..8270
                     /label=SFFV Pr
     primer_bind     8176..8199
                     /label=1166_seq
     CDS             8298..9161
                     /label=PD1
     misc_feature    8298..8369
                     /label=SP
     misc_feature    8793..8876
                     /label=TM
     misc_feature    8793..8876
                     /label=TM of PD1
     misc_feature    8877..9161
                     /label=Cytosolic domain, PD1
     misc_feature    8910..9161
                     /label=Int
     misc_feature    8958..8975
                     /label=BTLA ITIM
                     /note="BTLA ITIM"
     misc_feature    9033..9050
                     /label=PD-1 ITSM
                     /note="PD-1 ITSM"
     CDS             9222..9938
                     /label=mGFP
     primer_bind     9992..10014
                     /label=1167_seq
     misc_feature    10001..10592
                     /label=WPRE
ORIGIN
        1 cgataccgtc gacctcgagg gaattaattc gagctcggta cctttaagac caatgactta
       61 caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat
      121 tcactcccaa cgaagacaag atctgctttt tgcttgtact gggtctctct ggttagacca
      181 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
      241 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
      301 atccctcaga cccttttagt cagtgtggaa aatctctagc agcatctaga attaattccg
      361 tgtattctat agtgtcacct aaatcgtatg tgtatgatac ataaggttat gtattaattg
      421 tagccgcgtt ctaacgacaa tatgtacaag cctaattgtg tagcatctgg cttactgaag
      481 cagaccctat catctctctc gtaaactgcc gtcagagtcg gtttggttgg acgaaccttc
      541 tgagtttctg gtaacgccgt cccgcacccg gaaatggtca gcgaaccaat cagcagggtc
      601 atcgctagcc agatcctcta cgccggacgc atcgtggccg gcatcaccgg cgccacaggt
      661 gcggttgctg gcgcctatat cgccgacatc accgatgggg aagatcgggc tcgccacttc
      721 gggctcatga gcgcttgttt cggcgtgggt atggtggcag gccccgtggc cgggggactg
      781 ttgggcgcca tctccttgca tgcaccattc cttgcggcgg cggtgctcaa cggcctcaac
      841 ctactactgg gctgcttcct aatgcaggag tcgcataagg gagagcgtcg aatggtgcac
      901 tctcagtaca atctagctct gatgccgcat agttaagcca gccccgacac ccgccaacac
      961 ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga
     1021 ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac
     1081 gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt
     1141 agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     1201 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     1261 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
     1321 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
     1381 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
     1441 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
     1501 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact
     1561 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
     1621 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
     1681 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
     1741 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
     1801 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
     1861 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
     1921 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
     1981 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
     2041 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
     2101 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
     2161 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
     2221 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     2281 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     2341 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     2401 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc
     2461 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     2521 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     2581 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     2641 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     2701 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     2761 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     2821 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     2881 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     2941 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
     3001 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
     3061 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
     3121 ttcattaatg cagctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc
     3181 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc
     3241 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat
     3301 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
     3361 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga
     3421 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagcttgg
     3481 acacaagaca ggcttgcgag atatgtttga gaataccact ttatcccgcg tcagggagag
     3541 gcagtgcgta aaaagacgcg gactcatgtg aaatactggt ttttagtgcg ccagatctct
     3601 ataatctcgc gcaacctatt ttcccctcga acacttttta agccgtagat aaacaggctg
     3661 ggacacttca catgagcgaa aaatacatcg tcacctggga catgttgcag atccatgcac
     3721 gtaaactcgc aagccgactg atgccttctg aacaatggaa aggcattatt gccgtaagcc
     3781 gtggcggtct gtaccgggtg cgttactggc gcgtgaactg ggtattcgtc atgtcgatac
     3841 cgtttgtatt tccagctacg atcacgacaa ccagcgcgag cttaaagtgc tgaaacgcgc
     3901 agaaggcgat ggcgaaggct tcatcgttat tgatgacctg gtggataccg gtggtactgc
     3961 ggttgcgatt cgtgaaatgt atccaaaagc gcactttgtc accatcttcg caaaaccggc
     4021 tggtcgtccg ctggttgatg actatgttgt tgatatcccg caagatacct ggattgaaca
     4081 gccgtgggat atgggcgtcg tattcgtccc gccaatctcc ggtcgctaat cttttcaacg
     4141 cctggcactg ccgggcgttg ttctttttaa cttcaggcgg gttacaatag tttccagtaa
     4201 gtattctgga ggctgcatcc atgacacagg caaacctgag cgaaaccctg ttcaaacccc
     4261 gctttaaaca tcctgaaacc tcgacgctag tccgccgctt taatcacggc gcacaaccgc
     4321 ctgtgcagtc ggcccttgat ggtaaaacca tccctcactg gtatcgcatg attaaccgtc
     4381 tgatgtggat ctggcgcggc attgacccac gcgaaatcct cgacgtccag gcacgtattg
     4441 tgatgagcga tgccgaacgt accgacgatg atttatacga tacggtgatt ggctaccgtg
     4501 gcggcaactg gatttatgag tgggccccgg atctttgtga aggaacctta cttctgtggt
     4561 gtgacataat tggacaaact acctacagag atttaaagct ctaaggtaaa tataaaattt
     4621 ttaagtgtat aatgtgttaa actactgatt ctaattgttt gtgtatttta gattccaacc
     4681 tatggaactg atgaatggga gcagtggtgg aatgccttta atgaggaaaa cctgttttgc
     4741 tcagaagaaa tgccatctag tgatgatgag gctactgctg actctcaaca ttctactcct
     4801 ccaaaaaaga agagaaaggt agaagacccc aaggactttc cttcagaatt gctaagtttt
     4861 ttgagtcatg ctgtgtttag taatagaact cttgcttgct ttgctattta caccacaaag
     4921 gaaaaagctg cactgctata caagaaaatt atggaaaaat attctgtaac ctttataagt
     4981 aggcataaca gttataatca taacatactg ttttttctta ctccacacag gcatagagtg
     5041 tctgctatta ataactatgc tcaaaaattg tgtaccttta gctttttaat ttgtaaaggg
     5101 gttaataagg aatatttgat gtatagtgcc ttgactagag atcataatca gccataccac
     5161 atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca
     5221 taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata
     5281 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
     5341 tttgtccaaa ctcatcaatg tatcttatca tgtctggatc aactggataa ctcaagctaa
     5401 ccaaaatcat cccaaacttc ccaccccata ccctattacc actgccaatt acctagtggt
     5461 ttcatttact ctaaacctgt gattcctctg aattattttc attttaaaga aattgtattt
     5521 gttaaatatg tactacaaac ttagtagttg gaagggctaa ttcactccca aagaagacaa
     5581 gatatccttg atctgtggat ctaccacaca caaggctact tccctgatta gcagaactac
     5641 acaccagggc caggggtcag atatccactg acctttggat ggtgctacaa gctagtacca
     5701 gttgagccag ataaggtaga agaggccaat aaaggagaga acaccagctt gttacaccct
     5761 gtgagcctgc atgggatgga tgacccggag agagaagtgt tagagtggag gtttgacagc
     5821 cgcctagcat ttcatcacgt ggcccgagag ctgcatccgg agtacttcaa gaactgctga
     5881 tatcgagctt gctacaaggg actttccgct ggggactttc cagggaggcg tggcctgggc
     5941 gggactgggg agtggcgagc cctcagatcc tgcatataag cagctgcttt ttgcctgtac
     6001 tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc
     6061 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt
     6121 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag
     6181 cagtggcgcc cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc
     6241 aggactcggc ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg
     6301 ccaaaaattt tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt
     6361 aagcggggga gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa
     6421 aaatataaat taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat
     6481 cctggcctgt tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc
     6541 cttcagacag gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt
     6601 gtgcatcaaa ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag
     6661 caaaacaaaa gtaagaccac cgcacagcaa gcggccggtg atcttcagac ctggacgata
     6721 tatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca
     6781 ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg
     6841 ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg
     6901 tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac
     6961 aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc
     7021 aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg
     7081 gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt
     7141 tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga
     7201 gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa
     7261 gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt
     7321 aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta
     7381 ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca
     7441 ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata
     7501 gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa cggatctcga
     7561 cggtatcgcc aaatggcagt attcatccac aattttaaaa gaaaaggggg gattgggggg
     7621 tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta
     7681 caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag cagagatcca
     7741 gtttggatcg ataagcttga tatcgaattc ctgcagcccc gataaaataa aagattttat
     7801 ttagtctcca gaaaaagggg ggaatgaaag accccacctg taggtttggc aagctagctg
     7861 cagtaacgcc attttgcaag gcatggaaaa ataccaaacc aagaatagag aagttcagat
     7921 caagggcggg tacatgaaaa tagctaacgt tgggccaaac aggatatctg cggtgagcag
     7981 tttcggcccc ggcccggggc caagaacaga tggtcaccgc agtttcggcc ccggcccgag
     8041 gccaagaaca gatggtcccc agatatggcc caaccctcag cagtttctta agacccatca
     8101 gatgtttcca ggctccccca aggacctgaa atgaccctgc gccttatttg aattaaccaa
     8161 tcagcctgct tctcgcttct gttcgcgcgc ttctgcttcc cgagctctat aaaagagctc
     8221 acaacccctc actcggcgcg ccagtcctcc gacagactga gtcgcccggg ggggatctgg
     8281 agctctcgag aattctcatg cagatcccac aggcgccctg gccagtcgtc tgggcggtgc
     8341 tacaactggg ctggcggcca ggatggttct tagactcccc agacaggccc tggaaccccc
     8401 ccaccttctc cccagccctg ctcgtggtga ccgaagggga caacgccacc ttcacctgca
     8461 gcttctccaa cacatcggag agcttcgtgc taaactggta ccgcatgagc cccagcaacc
     8521 agacggacaa gctggccgct ttccccgagg accgcagcca gcccggccag gactgccgct
     8581 tccgtgtcac acaactgccc aacgggcgtg acttccacat gagcgtggtc agggcccggc
     8641 gcaatgacag cggcacctac ctctgtgggg ccatctccct ggcccccaag gcgcagatca
     8701 aagagagcct gcgggcagag ctcagggtga cagagagaag ggcagaagtg cccacagccc
     8761 accccagccc ctcacccagg ccagccggcc agttccaaac cctggtggtt ggtgtcgtgg
     8821 gcggcctgct gggcagcctg gtgctgctag tctgggtcct ggccgtcatc tgctcccggg
     8881 ccgcacgagg gacaatagga gccaggcgca ccggccagcc cctgaaggag gacccctcag
     8941 ccgtgcctgt gttctctatt gtttatgctt ccctggattt ccagtggcga gagaagaccc
     9001 cggagccccc cgtgccctgt gtccctgagc agacggagta tgccaccatt gtctttccta
     9061 gcggaatggg cacctcatcc cccgcccgca ggggctcagc cgacggccct cggagtgccc
     9121 agccactgag gcctgaggat ggacactgct cttggcccct gacgcgtctg caggatatca
     9181 agcttgcggt accgcgggcc cgggatccac cggtcgccac catggtgagc aagggcgagg
     9241 agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca
     9301 agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt
     9361 tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgacct
     9421 acggcgtgca gtgcttcagc cgctaccccg accacatgaa gcagcacgac ttcttcaagt
     9481 ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact
     9541 acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga
     9601 agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca
     9661 acagccacaa cgtctatatc atggccgaca agcagaagaa cggcatcaag gtgaacttca
     9721 agatccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca
     9781 cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtcca
     9841 agctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg
     9901 ccgccgggat cactctcggc atggacgagc tgtacaagta aagcggccgc gactctagag
     9961 tcgacctgca ggcatgcaag cttgatatca agcttatcga taatcaacct ctggattaca
    10021 aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat
    10081 acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct
    10141 ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac
    10201 gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca
    10261 cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca
    10321 tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg
    10381 tggtgttgtc ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt gccacctgga
    10441 ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt
    10501 cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga
    10561 gtcggatctc cctttgggcc gcctccccgc at
//
