LOCUS       pET28-SpRY-NLS-6xHis_(pMM-gbr2101)        9416 bp    DNA     circular UNA 05-JAN-2022
DEFINITION  .
ACCESSION   urn.local...ei-eb06v2k
VERSION     urn.local...ei-eb06v2k
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     source          1..9416
     misc_feature    19..35
                     /note="Geneious type: promoter prokaryotic"
                     /standard_name="T7 promoter"
     regulatory      38..62
                     /standard_name="lac operator"
     regulatory      82..98
                     /standard_name="T7 RBS"
     CDS             105..119
                     /standard_name="MKIEE MBP tag"
     CDS             117..4220
                     /standard_name="SpRY"
     CDS             4221..4244
                     /standard_name="gs linker"
     CDS             4245..4295
                     /standard_name="BPNLS(SV40)"
     CDS             4296..4313
                     /standard_name="6xHis Tag"
     regulatory      4323..4451
                     /note="Geneious type: terminator"
                     /standard_name="T7 transcriptional terminator"
     rep_origin      4463..4918
     misc_feature    complement(5011..5826)
                     /note="Geneious type: ORF"
                     /standard_name="KanR"
     rep_origin      5922..6595
     CDS             complement(7966..9048)
                     /standard_name="lacI CDS"
ORIGIN      
        1 atctcgatcc cgcgaaatta atacgactca ctatagggga attgtgagcg gataacaatt
       61 cccctctaga aataattttg tttaacttta agaaggagat atacatgaaa atcgaagaag
      121 acaagaagta ctcgatcgga ttggacattg gaaccaattc agtcggctgg gccgttatta
      181 ccgacgagta taaagttcct tccaagaagt ttaaggtgct tggaaataca gaccgccaca
      241 gtattaagaa gaatttgatc ggtgcgcttc ttttcgacag cggcgagacc gctgagcgta
      301 ctcgtctcaa acggacagct cgtagaaggt atacacgtcg gaagaatcgt atttgttatc
      361 tacaggagat tttttcaaat gagatggcga aagtagatga tagtttcttt catcgacttg
      421 aagagtcttt tttggtggaa gaagacaaga agcatgaacg tcatcctatt tttggaaata
      481 tagtagatga agttgcttat catgagaaat atccaactat ctatcatctg cgaaaaaaat
      541 tggtagattc tactgataaa gcggatttgc gcttaatcta tttggcctta gcgcatatga
      601 ttaagtttcg tggtcatttt ttgattgagg gagatttaaa tcctgataat agtgatgtgg
      661 acaaactatt tatccagttg gtacaaacct acaatcaatt atttgaagaa aaccctatta
      721 acgcaagtgg agtagatgct aaagcgattc tttctgcacg attgagtaaa tcaagacgat
      781 tagaaaatct cattgctcag ctccccggtg agaagaaaaa tggcttattt gggaatctca
      841 ttgctttgtc attgggtttg acccctaatt ttaaatcaaa ttttgatttg gcagaagatg
      901 ctaaattaca gctttcaaaa gatacttacg atgatgattt agataattta ttggcgcaaa
      961 ttggagatca atatgctgat ttgtttttgg cagctaagaa tttatcagat gctattttac
     1021 tttcagatat cctaagagta aatactgaaa taactaaggc tcccctatca gcttcaatga
     1081 ttaaacgcta cgatgaacat catcaagact tgactctttt aaaagcttta gttcgacaac
     1141 aacttccaga aaagtataaa gaaatctttt ttgatcaatc aaaaaacgga tatgcaggtt
     1201 atattgatgg gggagctagc caagaagaat tttataaatt tatcaaacca attttagaaa
     1261 aaatggatgg tactgaggaa ttattggtga aactaaatcg tgaagatttg ctgcgcaagc
     1321 aacggacctt tgacaacggc tctattcccc atcaaattca cttgggtgag ctgcatgcta
     1381 ttttgagaag acaagaagac ttttatccat ttttaaaaga caatcgtgag aagattgaaa
     1441 aaatcttgac ttttcgaatt ccttattatg ttggtccatt ggcgcgtggc aatagtcgtt
     1501 ttgcatggat gactcggaag tctgaagaaa caattacccc atggaatttt gaagaagttg
     1561 tcgataaagg tgcttcagct caatcattta ttgaacgcat gacaaacttt gataaaaatc
     1621 ttccaaatga aaaagtacta ccaaaacata gtttgcttta tgagtatttt acggtttata
     1681 acgaattgac aaaggtcaaa tatgttactg aaggaatgcg aaaaccagca tttctttcag
     1741 gtgaacagaa gaaagccatt gttgatttac tcttcaaaac aaatcgaaaa gtaaccgtta
     1801 agcaattaaa agaagattat ttcaaaaaaa tagaatgttt tgatagtgtt gaaatttcag
     1861 gagttgaaga tagatttaat gcttcattag gtacctacca tgatttgcta aaaattatta
     1921 aagataaaga ttttttggat aatgaagaaa atgaagatat cttagaggat attgttttaa
     1981 cattgacctt atttgaagat agggagatga ttgaggaaag acttaaaaca tatgctcacc
     2041 tctttgatga taaggtgatg aaacagctta aacgtcgccg ttatactggt tggggacgtt
     2101 tgtctcgaaa attgattaat ggtattaggg ataagcaatc tggcaaaaca atattagatt
     2161 ttttgaaatc agatggtttt gccaatcgca attttatgca gctgatccat gatgatagtt
     2221 tgacatttaa agaagacatt caaaaagcac aagtgtctgg acaaggcgat agtttacatg
     2281 aacatattgc aaatttagct ggtagccctg ctattaaaaa aggtatttta cagactgtaa
     2341 aagttgttga tgaattggtc aaagtaatgg ggcggcataa gccagaaaat atcgttattg
     2401 aaatggcacg tgaaaatcag acaactcaaa agggccagaa aaattcgcga gagcgtatga
     2461 aacgaatcga agaaggtatc aaagaattag gaagtcagat tcttaaagag catcctgttg
     2521 aaaatactca attgcaaaat gaaaagctct atctctatta tctccaaaat ggaagagaca
     2581 tgtatgtgga ccaagaatta gatattaatc gtttaagtga ttatgatgtc gatcacattg
     2641 ttccacaaag tttccttaaa gacgattcaa tagacaataa ggtcttaacg cgttctgata
     2701 aaaatcgtgg taaatcggat aacgttccaa gtgaagaagt agtcaaaaag atgaaaaact
     2761 attggagaca acttctaaac gccaagttaa tcactcaacg taagtttgat aatttaacga
     2821 aagctgaacg tggaggtttg agtgaacttg ataaagctgg ttttatcaaa cgccaattgg
     2881 ttgaaactcg ccaaatcact aagcatgtgg cacaaatttt ggatagtcgc atgaatacta
     2941 aatacgatga aaatgataaa cttattcgag aggttaaagt gattacctta aaatctaaat
     3001 tagtttctga cttccgaaaa gatttccaat tctataaagt acgtgagatt aacaattacc
     3061 atcatgccca tgatgcgtat ctaaatgccg tcgttggaac tgctttgatt aagaaatatc
     3121 caaaacttga atcggagttt gtctatggtg attataaagt ttatgatgtt cgtaaaatga
     3181 ttgctaagtc tgagcaagaa ataggcaaag caaccgcaaa atatttcttt tactctaata
     3241 tcatgaactt cttcaaaaca gaaattacac ttgcaaatgg agagattcgc aaacgccctc
     3301 taatcgaaac taatggggaa actggagaaa ttgtctggga taaagggcga gattttgcca
     3361 cagtgcgcaa agtattgtcc atgccccaag tcaatattgt caagaaaaca gaagtacaga
     3421 caggcggatt ctccaaggag tcaattcgcc ctaagcgcaa ctcagataag cttattgcgc
     3481 gtaagaaaga ctgggatccc aagaagtacg gcgggttcct ctggcccacg gtggcgtaca
     3541 gcgttcttgt tgttgcaaag gtagagaagg gcaagagtaa gaagctgaag tccgtaaagg
     3601 agctgcttgg catcaccatc atggaacgct cttcttttga gaagaaccct atcgacttct
     3661 tagaagctaa gggctataag gaagttaaga aagacctgat catcaagctt cccaagtatt
     3721 ctttattcga gttggagaat ggccgcaagc gtatgctggc gtcggcaaag caactgcaga
     3781 agggtaatga gttggccctg ccgagcaagt acgttaattt tctgtattta gcgagtcact
     3841 atgagaagtt aaaaggaagc cccgaggaca atgagcagaa acaactgttc gtcgagcaac
     3901 ataagcacta cttggatgag attattgagc agattagcga gttctcaaag cgtgtaatct
     3961 tagcagatgc taacttggat aaggttctga gcgcctacaa caagcatcgc gataagccta
     4021 tccgcgaaca ggcggagaat atcattcacc tgttcactct cacacgtttg ggcgcacccc
     4081 gtgcctttaa atatttcgac accacgatcg accctaagca atatcgcagt accaaagagg
     4141 tgctggatgc cacccttatc catcaaagca tcaccgggtt gtatgagacc cgtatcgatc
     4201 ttagccagtt aggcggggac gggtcgggcg gcggcggctc gggcaagcgt acagccgacg
     4261 gaagtgagtt cgagcctaaa aagaagcgta aggtacatca ccatcaccat cattaaatcc
     4321 ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact
     4381 agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac
     4441 tatatccgga ttggcgaatg ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg
     4501 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct
     4561 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg
     4621 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag
     4681 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg
     4741 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc
     4801 tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat
     4861 gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt tacaatttca
     4921 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
     4981 tcaaatatgt atccgctcat gaattaattc ttagaaaaac tcatcgagca tcaaatgaaa
     5041 ctgcaattta ttcatatcag gattatcaat accatatttt tgaaaaagcc gtttctgtaa
     5101 tgaaggagaa aactcaccga ggcagttcca taggatggca agatcctggt atcggtctgc
     5161 gattccgact cgtccaacat caatacaacc tattaatttc ccctcgtcaa aaataaggtt
     5221 atcaagtgag aaatcaccat gagtgacgac tgaatccggt gagaatggca aaagtttatg
     5281 catttctttc cagacttgtt caacaggcca gccattacgc tcgtcatcaa aatcactcgc
     5341 atcaaccaaa ccgttattca ttcgtgattg cgcctgagcg agacgaaata cgcgatcgct
     5401 gttaaaagga caattacaaa caggaatcga atgcaaccgg cgcaggaaca ctgccagcgc
     5461 atcaacaata ttttcacctg aatcaggata ttcttctaat acctggaatg ctgttttccc
     5521 ggggatcgca gtggtgagta accatgcatc atcaggagta cggataaaat gcttgatggt
     5581 cggaagaggc ataaattccg tcagccagtt tagtctgacc atctcatctg taacatcatt
     5641 ggcaacgcta cctttgccat gtttcagaaa caactctggc gcatcgggct tcccatacaa
     5701 tcgatagatt gtcgcacctg attgcccgac attatcgcga gcccatttat acccatataa
     5761 atcagcatcc atgttggaat ttaatcgcgg cctagagcaa gacgtttccc gttgaatatg
     5821 gctcataaca ccccttgtat tactgtttat gtaagcagac agttttattg ttcatgacca
     5881 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     5941 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     6001 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     6061 ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc
     6121 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     6181 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     6241 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     6301 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     6361 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     6421 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     6481 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     6541 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     6601 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
     6661 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
     6721 gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg
     6781 cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg
     6841 ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga
     6901 cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc
     6961 atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca
     7021 tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg
     7081 agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt
     7141 ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg
     7201 ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg
     7261 ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa
     7321 atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc
     7381 cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt
     7441 tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac
     7501 gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca
     7561 gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc
     7621 cgtggggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga
     7681 ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg
     7741 atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc
     7801 acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg
     7861 ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgagat
     7921 cccggtgcct aatgagtgag ctaacttaca ttaattgcgt tgcgctcact gcccgctttc
     7981 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc
     8041 ggtttgcgta ttgggcgcca gggtggtttt tcttttcacc agtgagacgg gcaacagctg
     8101 attgcccttc accgcctggc cctgagagag ttgcagcaag cggtccacgc tggtttgccc
     8161 cagcaggcga aaatcctgtt tgatggtggt taacggcggg atataacatg agctgtcttc
     8221 ggtatcgtcg tatcccacta ccgagatatc cgcaccaacg cgcagcccgg actcggtaat
     8281 ggcgcgcatt gcgcccagcg ccatctgatc gttggcaacc agcatcgcag tgggaacgat
     8341 gccctcattc agcatttgca tggtttgttg aaaaccggac atggcactcc agtcgccttc
     8401 ccgttccgct atcggctgaa tttgattgcg agtgagatat ttatgccagc cagccagacg
     8461 cagacgcgcc gagacagaac ttaatgggcc cgctaacagc gcgatttgct ggtgacccaa
     8521 tgcgaccaga tgctccacgc ccagtcgcgt accgtcttca tgggagaaaa taatactgtt
     8581 gatgggtgtc tggtcagaga catcaagaaa taacgccgga acattagtgc aggcagcttc
     8641 cacagcaatg gcatcctggt catccagcgg atagttaatg atcagcccac tgacgcgttg
     8701 cgcgagaaga ttgtgcaccg ccgctttaca ggcttcgacg ccgcttcgtt ctaccatcga
     8761 caccaccacg ctggcaccca gttgatcggc gcgagattta atcgccgcga caatttgcga
     8821 cggcgcgtgc agggccagac tggaggtggc aacgccaatc agcaacgact gtttgcccgc
     8881 cagttgttgt gccacgcggt tgggaatgta attcagctcc gccatcgccg cttccacttt
     8941 ttcccgcgtt ttcgcagaaa cgtggctggc ctggttcacc acgcgggaaa cggtctgata
     9001 agagacaccg gcatactctg cgacatcgta taacgttact ggtttcacat tcaccaccct
     9061 gaattgactc tcttccgggc gctatcatgc cataccgcga aaggttttgc gccattcgat
     9121 ggtgtccggg atctcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta
     9181 gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc
     9241 ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga
     9301 gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa
     9361 ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgag
//
