LOCUS       AiP993_-_TIT2L-G       18236 bp    DNA     circular UNA 24-MAR-2021
DEFINITION  .
ACCESSION   urn.local...11j-4yy8378
VERSION     urn.local...11j-4yy8378
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     misc_feature    25..97
                     /standard_name="prokaryotic gb2 promoter"
     CDS             98..901
                     /standard_name="NeoR"
     misc_recomb     980..1279
                     /standard_name="Bxb1 attB"
     CDS             <1302..>1798
                     /standard_name="GFP partial"
     misc_feature    1819..3027
                     /standard_name="Insulator\1"
                     /modified_by="laakeas"
                     /label="Insulator1"
     misc_feature    3040..4243
                     /standard_name="Insulator\2"
                     /modified_by="laakeas"
                     /label="Insulator2"
     misc_feature    4302..4729
                     /standard_name="TRE2\from\pTRE2-Hyg"
                     /modified_by="jacqueline.bendrick"
                     /label="TRE2"
     Misc_binding    4310..4328
                     /standard_name="tetO"
                     /modified_by="jacqueline.bendrick"
     Misc_binding    4352..4370
                     /standard_name="tetO"
                     /modified_by="jacqueline.bendrick"
     Misc_binding    4394..4412
                     /standard_name="tetO"
                     /modified_by="jacqueline.bendrick"
     Misc_binding    4436..4454
                     /standard_name="tetO"
                     /modified_by="jacqueline.bendrick"
     Misc_binding    4478..4496
                     /standard_name="tetO"
                     /modified_by="jacqueline.bendrick"
     Misc_binding    4520..4538
                     /standard_name="tetO"
                     /modified_by="jacqueline.bendrick"
     Misc_binding    4562..4580
                     /standard_name="tetO"
                     /modified_by="jacqueline.bendrick"
     promoter        4615..4674
                     /standard_name="pCMV\min"
                     /modified_by="laakeas"
                     /label="pCMVmin"
     misc_recomb     complement(4785..4818)
                     /standard_name="LoxP"
     misc_feature    4826..4836
                     /standard_name="Stops"
     misc_feature    4885..4895
                     /standard_name="Stops"
     misc_feature    4896..4945
                     /standard_name="Synthetic\pA"
                     /modified_by="laakeas"
                     /label="Synthetic pA"
     polyA_signal    4946..5432
                     /modified_by="laakeas"
                     /label="hGHpA"
     polyA_signal    5440..5907
                     /modified_by="laakeas"
                     /label="PGKpA"
     misc_feature    5917..5927
                     /standard_name="Stops"
     misc_recomb     complement(5929..5962)
                     /standard_name="LoxP"
     CDS             5979..7331
                     /codon_start=1
                     /product="improved fluorescent protein-based calcium
                     sensor (Chen et al., 2013)"
                     /translation="MGSHHHHHHGMASMTGGQQMGRDLYDDDDKDLATMVDSSRRKWN
                     KTGHAVRVIGRLSSLENVYIKADKQKNGIKANFHIRHNIEDGGVQLAYHYQQNTPIGD
                     GPVLLPDNHYLSVQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYKGGTGGSMVSK
                     GEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLV
                     TTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFKDDGNYKTRAEVKFEGDTL
                     VNRIELKGIDFKEDGNILGHKLEYNLPDQLTEEQIAEFKELFSLFDKDGDGTITTKEL
                     GTVMRSLGQNPTEAELQDMINEVDADGDGTIDFPEFLTMMARKMKYRDTEEEIREAFG
                     VFDKDGNGYISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTAK
                     "
                     /standard_name="GCaMP7s"
     CDS             5988..6005
                     /standard_name="6x His"
     CDS             6009..6041
                     /standard_name="T7 tag"
     CDS             6045..6068
                     /standard_name="XPress tag"
     misc_feature    7346..7954
                     /standard_name="WPRE"
     polyA_signal    7968..8182
                     /modified_by="laakeas"
                     /label="bGHpA"
     misc_feature    8206..9409
                     /standard_name="Insulator\3"
                     /modified_by="laakeas"
                     /label="Insulator3"
     misc_feature    9416..10619
                     /standard_name="Insulator\4"
                     /modified_by="laakeas"
                     /label="Insulator4"
     misc_feature    10647..12394
                     /standard_name="CAG"
     misc_recomb     12401..12434
                     /standard_name="FRT"
     misc_feature    12469..12479
                     /standard_name="Stops"
     misc_feature    12480..12529
                     /standard_name="Synthetic pA"
     polyA_signal    12530..13016
                     /modified_by="laakeas"
                     /label="hGHpA"
     misc_feature    13024..13034
                     /standard_name="Stops"
     misc_feature    13035..13296
                     /standard_name="TK pA"
     misc_recomb     13300..13333
                     /standard_name="FRT"
     misc_feature    13349..13929
                     /standard_name="IRES"
     CDS             13939..14685
                     /created_by="laakeas"
                     /label="tTA2"
     misc_feature    14718..15326
                     /standard_name="WPRE"
     polyA_signal    15340..15554
                     /modified_by="laakeas"
                     /label="bGHpA"
     misc_recomb     15567..15601
                     /standard_name="AttB PhiC31"
                     /modified_by="jacqueline.bendrick"
                     /label="attB PhiC31"
     promoter        15615..16140
                     /standard_name="PGK"
     CDS             16154..16666
                     /standard_name="Hygro\1"
                     /modified_by="laakeas"
                     /label="Hygro1"
     misc_feature    16667..16672
                     /created_by="jacqueline.bendrick"
                     /label="Slice donor"
     misc_recomb     16694..16993
                     /standard_name="Bxb1 attB"
     rep_origin      17125..17791
     CDS             17942..>18236
                     /standard_name="Amp"
ORIGIN      
        1 aacgttttgg cggtttaaac attaagcacg tgttgacaat taatcatcgg catagtatat
       61 cggcatagta taatacgaca aggtgaggaa ctaaaccatg ggatcggcca ttgaacaaga
      121 tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc
      181 acaacagacg atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc
      241 ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcagg acgaggcagc
      301 gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac
      361 tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc
      421 tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac
      481 gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg
      541 tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct
      601 cgcgccagcc gaactgttcg ccaggctcaa ggcgcgcatg cccgacggcg aggatctcgt
      661 cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg
      721 attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac
      781 ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg
      841 tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg
      901 aagtgttaac tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag
      961 tgaattcgag ctcggtaccg aaaaaccgct cagcggcgcc cgcacccaaa cgaaaacacc
     1021 cccggcccaa gcggaccggg ggtgtcgcgt gaaatggatc agtgagcgtg gccgtggtgg
     1081 tggccgtggc cgtgctcgtc ctcgtcggcc ggcttgtcga cgacggcggt ctccgtcgtc
     1141 aggatcatcc gggccaccga ggcggcgttg agaacagccg agcgggtcac cttggccgga
     1201 tcgacgacgc cggcggagac cagatcaccg aactccagcg tggcggcgtt gaagccctgc
     1261 cccttgggaa gttcggagat ttcggccggc caatcgattt taccggggtg gtgcccatcc
     1321 tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg
     1381 gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg
     1441 tgccctggcc caccctcgtg accaccctga cctacggcgt gcagtgcttc agccgctacc
     1501 ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg
     1561 agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg
     1621 agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca
     1681 acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg
     1741 acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggtt
     1801 aattaagaat tcgagctcgc tagagcccat cctcactgac tccgtcctgg agttggatga
     1861 gagataatgg ccttacgttg tgccagggga gggtcgggct ggatttagca agatttacct
     1921 tctccaaaga gcggtgctgc agtggcacag ctgcccacgg aggtgggggg gtcaccgtcc
     1981 ctggaggtga tgaagaactg tggggatgtg gcactgaggg acatggccag tgggcacggt
     2041 gggtgggttg gggttggtct tggggatctt ggagggcttt tccagccttc atgatttgac
     2101 gattgtatga acatctacat ggcaattctc cagctgcctg tcccagtcct actgacccag
     2161 ctgtatctct ccaggcaagc tcttccaccc cttctgcttg catccagaca ccatcaaaca
     2221 tgcaggctca gacacaggga ccagcagtgt ctgtggcctt tttgtgctcc tctccatgct
     2281 gggttttaac ttgctctttg tccttctatc ctatcttctt atccttaagg ctgttctgaa
     2341 cgctgtgact tggagagtgt cccagagccc tcaacacctg catgtcccac gtccatgctg
     2401 tcctgcactt ccttatcccc aagatctgcc tctccgtgat gcactgaatt ggcaaacatg
     2461 tgtcacccca gaccaacaat gtcacagcaa actccccctt gataggacaa gggggaatgg
     2521 ctttacactg agacagggga ggtttgggtt ggatatgagg aggcagtttt tcccccagag
     2581 ggtggtgacg cactgaacag gttgcccaag gaggctgtgg atgccccatc cctgcaggca
     2641 ttcaaggcca ggctggatgt ggctctgggc agcctgggct gctggttgat gaccctgcac
     2701 atagcagggg gttggatctg gatgagcact gtgctccttt gcaacccagg ccgttctatg
     2761 attctgtcat tctaaatctc tctttcagcc taaagctttt tccccgtatc cccccaggtg
     2821 tctgcaggct caaagagcag cgagaagcgt tcagaggaaa gcgatcccgt gccaccttcc
     2881 ccgtgcccgg gctgtccccg cacgctgccg gctcggggat gcggggggag cgccggaccg
     2941 gagcggagcc ccgggcggct cgctgctgcc ccctagcggg ggagggacgt aattacatcc
     3001 ctgggggctt tggggggggg ctgtcccgag ctcggtaccg ccccatcctc actgactccg
     3061 tcctggagtt ggatgagaga taatggcctt acgttgtgcc aggggagggt cgggctggat
     3121 ttagcaagat ttaccttctc caaagagcgg tgctgcagtg gcacagctgc ccacggaggt
     3181 gggggggtca ccgtccctgg aggtgatgaa gaactgtggg gatgtggcac tgagggacat
     3241 ggccagtggg cacggtgggt gggttggggt tggtcttggg gatcttggag ggcttttcca
     3301 gccttcatga tttgacgatt gtatgaacat ctacatggca attctccagc tgcctgtccc
     3361 agtcctactg acccagctgt atctctccag gcaagctctt ccaccccttc tgcttgcatc
     3421 cagacaccat caaacatgca ggctcagaca cagggaccag cagtgtctgt ggcctttttg
     3481 tgctcctctc catgctgggt tttaacttgc tctttgtcct tctatcctat cttcttatcc
     3541 ttaaggctgt tctgaacgct gtgacttgga gagtgtccca gagccctcaa cacctgcatg
     3601 tcccacgtcc atgctgtcct gcacttcctt atccccaaga tctgcctctc cgtgatgcac
     3661 tgaattggca aacatgtgtc accccagacc aacaatgtca cagcaaactc ccccttgata
     3721 ggacaagggg gaatggcttt acactgagac aggggaggtt tgggttggat atgaggaggc
     3781 agtttttccc ccagagggtg gtgacgcact gaacaggttg cccaaggagg ctgtggatgc
     3841 cccatccctg caggcattca aggccaggct ggatgtggct ctgggcagcc tgggctgctg
     3901 gttgatgacc ctgcacatag cagggggttg gatctggatg agcactgtgc tcctttgcaa
     3961 cccaggccgt tctatgattc tgtcattcta aatctctctt tcagcctaaa gctttttccc
     4021 cgtatccccc caggtgtctg caggctcaaa gagcagcgag aagcgttcag aggaaagcga
     4081 tcccgtgcca ccttccccgt gcccgggctg tccccgcacg ctgccggctc ggggatgcgg
     4141 ggggagcgcc ggaccggagc ggagccccgg gcggctcgct gctgccccct agcgggggag
     4201 ggacgtaatt acatccctgg gggctttggg ggggggctgt cccggtaccc ggggatccac
     4261 cggtcgtaag cggttataag ctgcaataaa caagtttcga gtttaccact ccctatcagt
     4321 gatagagaaa agtgaaagtc gagtttacca ctccctatca gtgatagaga aaagtgaaag
     4381 tcgagtttac cactccctat cagtgataga gaaaagtgaa agtcgagttt accactccct
     4441 atcagtgata gagaaaagtg aaagtcgagt ttaccactcc ctatcagtga tagagaaaag
     4501 tgaaagtcga gtttaccact ccctatcagt gatagagaaa agtgaaagtc gagtttacca
     4561 ctccctatca gtgatagaga aaagtgaaag tcgagctcgg tacccgggtc gaggtaggcg
     4621 tgtacggtgg gaggcctata taagcagagc tcgtttagtg aaccgtcaga tcgcctggag
     4681 acgccatcca cgctgttttg acctccatag aagacaccgg gaccgatcca gcctccgcgg
     4741 ccccgaattc gagctcggta cccggggatc aaattcaatt aaatataact tcgtatagca
     4801 tacattatac gaagttattc gcgagtgaat aaatgaaagc ttgcagatcc cctcagcacc
     4861 atggctagcg gcagcctcgg agtttgaata gatagaataa aatatcttta ttttcattcc
     4921 atctgtgtgt tggttttttg tgtgagatct acgggtggca tccctgtgac ccctccccag
     4981 tgcctctcct ggccctggaa gttgccactc cagtgcccac cagccttgtc ctaataaaat
     5041 taagttgcat cattttgtct gactaggtgt ccttctataa tattatgggg tggagggggg
     5101 tggtatggag caaggggcaa gttgggaaga caacctgtag ggcctgcggg gtctattggg
     5161 aaccaagctg gagtgcagtg gcacaatctt ggctcactgc aatctccgcc tcctgggttc
     5221 aagcgattct cctgcctcag gctcccgagt tgttgggatt ccaggcatgc atgaccaggc
     5281 tcagctaatt tttgtttttt tggtagagac ggggtttcac catattggcc aggctggtct
     5341 ccaactccta atctcaggtg atctacccac cttggcctcc caaattgctg ggattacagg
     5401 cgtgaaccac tgctcccttc cctgtccttc tgcctcagcg ctgtaagtct gcagaaattg
     5461 atgatctatt aaacaataaa gatgtccact aaaatggaag tttttcctgt catactttgt
     5521 taagaagggt gagaacagag tacctacatt ttgaatggaa ggattggagc tacgggggtg
     5581 ggggtggggt gggattagat aaatgcctgc tctttactga aggctcttta ctattgcttt
     5641 atgataatgt ttcatagttg gatatcataa tttaaacaag caaaaccaaa ttaagggcca
     5701 gctcattcct cccactcatg atctatagat ctatagatct ctcgtgggat cattgttttt
     5761 ctcttgattc ccactttgtg gttctaagta ctgtggtttc caaatgtgtc agtttcatag
     5821 cctgaagaac gagatcagca gcctctgttc cacatacact tcattctcag tattgttttg
     5881 ccaagttcta attccatcag aaagctttta cttaactgaa tagctaacat aacttcgtat
     5941 agcatacatt atacgaagtt atcacgcgtg ccgccaccat gggttctcat catcatcatc
     6001 atcatggtat ggctagcatg actggtggac agcaaatggg tcgggatctg tacgacgatg
     6061 acgataagga tctcgccacc atggtcgact catcacgtcg taagtggaat aagacaggtc
     6121 acgcagtcag agtgataggt cggctgagct cactcgagaa cgtctatatc aaggccgaca
     6181 agcagaagaa cggcatcaag gcgaacttcc acatccgcca caacatcgag gacggcggcg
     6241 tgcagctcgc ctaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc
     6301 ccgacaacca ctacctgagc gtgcagtcca aactttcgaa agaccccaac gagaagcgcg
     6361 atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc
     6421 tgtacaaggg cggtaccgga gggagcatgg tgagcaaggg cgaggagctg ttcaccgggg
     6481 tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg
     6541 gcgagggtga gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg
     6601 gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctacggc gtgcagtgct
     6661 tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag
     6721 gctacatcca ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg
     6781 aggtgaagtt cgagggcgac accctggtga accgcatcga gcttaagggc atcgacttca
     6841 aggaggacgg caacatcctg gggcacaagc tggagtacaa ccttcctgac caactgactg
     6901 aagagcagat cgcagaattt aaagagcttt tctccctatt tgacaaggac ggggatggga
     6961 caataacaac caaggagctg gggacggtga tgcggtctct ggggcagaac cccacagaag
     7021 cagagctgca ggacatgatc aatgaagtag atgccgacgg tgacggcaca atcgacttcc
     7081 ctgagttcct gacaatgatg gcaagaaaaa tgaaatacag ggacacggaa gaagaaatta
     7141 gagaagcgtt cggtgtgttt gataaggatg gcaatggcta catcagtgca gcagagcttc
     7201 gccacgtgat gacaaacctt ggagagaagt taacagatga agaggttgat gaaatgatca
     7261 gggaagcaga catcgatggg gatggtcagg taaactacga agagtttgta caaatgatga
     7321 cagcgaagtg aacgcgttac gtaaagcttg tcgataatca acctctggat tacaaaattt
     7381 gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg
     7441 ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt
     7501 ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg
     7561 tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc
     7621 agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg
     7681 cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt
     7741 tgtcggggaa atcatcgtcc tttccttggc tgctcgccta tgttgccacc tggattctgc
     7801 gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg
     7861 gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga
     7921 tctccctttg ggccgcctcc ccgcatcgac accgagcgct gctcgaggcc tcgactgtgc
     7981 cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag
     8041 gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta
     8101 ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag
     8161 acaatagcag gcatgctggg gacacgtggg atcctctagt ctagagcccc atcctcactg
     8221 actccgtcct ggagttggat gagagataat ggccttacgt tgtgccaggg gagggtcggg
     8281 ctggatttag caagatttac cttctccaaa gagcggtgct gcagtggcac agctgcccac
     8341 ggaggtgggg gggtcaccgt ccctggaggt gatgaagaac tgtggggatg tggcactgag
     8401 ggacatggcc agtgggcacg gtgggtgggt tggggttggt cttggggatc ttggagggct
     8461 tttccagcct tcatgatttg acgattgtat gaacatctac atggcaattc tccagctgcc
     8521 tgtcccagtc ctactgaccc agctgtatct ctccaggcaa gctcttccac cccttctgct
     8581 tgcatccaga caccatcaaa catgcaggct cagacacagg gaccagcagt gtctgtggcc
     8641 tttttgtgct cctctccatg ctgggtttta acttgctctt tgtccttcta tcctatcttc
     8701 ttatccttaa ggctgttctg aacgctgtga cttggagagt gtcccagagc cctcaacacc
     8761 tgcatgtccc acgtccatgc tgtcctgcac ttccttatcc ccaagatctg cctctccgtg
     8821 atgcactgaa ttggcaaaca tgtgtcaccc cagaccaaca atgtcacagc aaactccccc
     8881 ttgataggac aagggggaat ggctttacac tgagacaggg gaggtttggg ttggatatga
     8941 ggaggcagtt tttcccccag agggtggtga cgcactgaac aggttgccca aggaggctgt
     9001 ggatgcccca tccctgcagg cattcaaggc caggctggat gtggctctgg gcagcctggg
     9061 ctgctggttg atgaccctgc acatagcagg gggttggatc tggatgagca ctgtgctcct
     9121 ttgcaaccca ggccgttcta tgattctgtc attctaaatc tctctttcag cctaaagctt
     9181 tttccccgta tccccccagg tgtctgcagg ctcaaagagc agcgagaagc gttcagagga
     9241 aagcgatccc gtgccacctt ccccgtgccc gggctgtccc cgcacgctgc cggctcgggg
     9301 atgcgggggg agcgccggac cggagcggag ccccgggcgg ctcgctgctg ccccctagcg
     9361 ggggagggac gtaattacat ccctgggggc tttggggggg ggctgtccct ctagagcccc
     9421 atcctcactg actccgtcct ggagttggat gagagataat ggccttacgt tgtgccaggg
     9481 gagggtcggg ctggatttag caagatttac cttctccaaa gagcggtgct gcagtggcac
     9541 agctgcccac ggaggtgggg gggtcaccgt ccctggaggt gatgaagaac tgtggggatg
     9601 tggcactgag ggacatggcc agtgggcacg gtgggtgggt tggggttggt cttggggatc
     9661 ttggagggct tttccagcct tcatgatttg acgattgtat gaacatctac atggcaattc
     9721 tccagctgcc tgtcccagtc ctactgaccc agctgtatct ctccaggcaa gctcttccac
     9781 cccttctgct tgcatccaga caccatcaaa catgcaggct cagacacagg gaccagcagt
     9841 gtctgtggcc tttttgtgct cctctccatg ctgggtttta acttgctctt tgtccttcta
     9901 tcctatcttc ttatccttaa ggctgttctg aacgctgtga cttggagagt gtcccagagc
     9961 cctcaacacc tgcatgtccc acgtccatgc tgtcctgcac ttccttatcc ccaagatctg
    10021 cctctccgtg atgcactgaa ttggcaaaca tgtgtcaccc cagaccaaca atgtcacagc
    10081 aaactccccc ttgataggac aagggggaat ggctttacac tgagacaggg gaggtttggg
    10141 ttggatatga ggaggcagtt tttcccccag agggtggtga cgcactgaac aggttgccca
    10201 aggaggctgt ggatgcccca tccctgcagg cattcaaggc caggctggat gtggctctgg
    10261 gcagcctggg ctgctggttg atgaccctgc acatagcagg gggttggatc tggatgagca
    10321 ctgtgctcct ttgcaaccca ggccgttcta tgattctgtc attctaaatc tctctttcag
    10381 cctaaagctt tttccccgta tccccccagg tgtctgcagg ctcaaagagc agcgagaagc
    10441 gttcagagga aagcgatccc gtgccacctt ccccgtgccc gggctgtccc cgcacgctgc
    10501 cggctcgggg atgcgggggg agcgccggac cggagcggag ccccgggcgg ctcgctgctg
    10561 ccccctagcg ggggagggac gtaattacat ccctgggggc tttggggggg ggctgtccct
    10621 ctagaagtcg acggcgcgcc ctgcaggaat ctagttatta atagtaatca attacggggt
    10681 cattagttca tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc
    10741 ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag
    10801 taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc
    10861 acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg
    10921 gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc
    10981 agtacatcta cgtattagtc atcgctatta ccatggtcga ggtgagcccc acgttctgct
    11041 tcactctccc catctccccc ccctccccac ccccaatttt gtatttattt attttttaat
    11101 tattttgtgc agcgatgggg gcgggggggg ggggggggcg cgcgccaggc ggggcggggc
    11161 ggggcgaggg gcggggcggg gcgaggcgga gaggtgcggc ggcagccaat cagagcggcg
    11221 cgctccgaaa gtttcctttt atggcgaggc ggcggcggcg gcggccctat aaaaagcgaa
    11281 gcgcgcggcg ggcggggagt cgctgcgacg ctgccttcgc cccgtgcccc gctccgccgc
    11341 cgcctcgcgc cgcccgcccc ggctctgact gaccgcgtta ctcccacagg tgagcgggcg
    11401 ggacggccct tctcctccgg gctgtaatta gcgcttggtt taatgacggc ttgtttcttt
    11461 tctgtggctg cgtgaaagcc ttgaggggct ccgggagggc cctttgtgcg gggggagcgg
    11521 ctcggggggt gcgtgcgtgt gtgtgtgcgt ggggagcgcc gcgtgcggct ccgcgctgcc
    11581 cggcggctgt gagcgctgcg ggcgcggcgc ggggctttgt gcgctccgca gtgtgcgcga
    11641 ggggagcgcg gccgggggcg gtgccccgcg gtgcgggggg ggctgcgagg ggaacaaagg
    11701 ctgcgtgcgg ggtgtgtgcg tgggggggtg agcagggggt gtgggcgcgt cggtcgggct
    11761 gcaacccccc ctgcaccccc ctccccgagt tgctgagcac ggcccggctt cgggtgcggg
    11821 gctccgtacg gggcgtggcg cggggctcgc cgtgccgggc ggggggtggc ggcaggtggg
    11881 ggtgccgggc ggggcggggc cgcctcgggc cggggagggc tcgggggagg ggcgcggcgg
    11941 cccccggagc gccggcggct gtcgaggcgc ggcgagccgc agccattgcc ttttatggta
    12001 atcgtgcgag agggcgcagg gacttccttt gtcccaaatc tgtgcggagc cgaaatctgg
    12061 gaggcgccgc cgcaccccct ctagcgggcg cggggcgaag cggtgcggcg ccggcaggaa
    12121 ggaaatgggc ggggagggcc ttcgtgcgtc gccgcgccgc cgtccccttc tccctctcca
    12181 gcctcggggc tgtccgcggg gggacggctg ccttcggggg ggacggggca gggcggggtt
    12241 cggcttctgg cgtgtgaccg gcggctctag agcctctgct aaccatgttc atgccttctt
    12301 ctttttccta cagctcctgg gcaacgtgct ggttattgtg ctgtctcatc attttggcaa
    12361 agaattgatt tgataccgcg ggccccgcgt ggacggtacc gaagttccta ttctctagaa
    12421 agtataggaa cttccctcag caccatggct agcggcagcc tcggagtttg aatagataga
    12481 ataaaatatc tttattttca ttccatctgt gtgttggttt tttgtgtgag atctacgggt
    12541 ggcatccctg tgacccctcc ccagtgcctc tcctggccct ggaagttgcc actccagtgc
    12601 ccaccagcct tgtcctaata aaattaagtt gcatcatttt gtctgactag gtgtccttct
    12661 ataatattat ggggtggagg ggggtggtat ggagcaaggg gcaagttggg aagacaacct
    12721 gtagggcctg cggggtctat tgggaaccaa gctggagtgc agtggcacaa tcttggctca
    12781 ctgcaatctc cgcctcctgg gttcaagcga ttctcctgcc tcaggctccc gagttgttgg
    12841 gattccaggc atgcatgacc aggctcagct aatttttgtt tttttggtag agacggggtt
    12901 tcaccatatt ggccaggctg gtctccaact cctaatctca ggtgatctac ccaccttggc
    12961 ctcccaaatt gctgggatta caggcgtgaa ccactgctcc cttccctgtc cttctgcctc
    13021 agctaattga gtagggggga ggctaactga aacacggaag gagacaatac cggaaggaac
    13081 ccgcgctatg acggcaataa aaagacagaa taaaacgcac gggtgttggg tcgtttgttc
    13141 ataaacgcgg ggttcggtcc cagggctggc actctgtcga taccccaccg agaccccatt
    13201 ggggccaata cgcccgcgtt tcttcctttt ccccacccca ccccccaagt tcgggtgaag
    13261 gcccagggct cgcagccaac gtcggggcgg caggccgtgg aagttcctat tctctagaaa
    13321 gtataggaac ttcacgcgcg cgtccattaa ttccgcccct ctccctcccc cccccctaac
    13381 gttactggcc gaagccgctt ggaataaggc cggtgtgcgt ttgtctatat gttattttcc
    13441 accatattgc cgtcttttgg caatgtgagg gcccggaaac ctggccctgt cttcttgacg
    13501 agcattccta ggggtctttc ccctctcgcc aaaggaatgc aaggtctgtt gaatgtcgtg
    13561 aaggaagcag ttcctctgga agcttcttga agacaaacaa cgtctgtagc gaccctttgc
    13621 aggcagcgga accccccacc tggcgacagg tgcctctgcg gccaaaagcc acgtgtataa
    13681 gatacacctg caaaggcggc acaaccccag tgccacgttg tgagttggat agttgtggaa
    13741 agagtcaaat ggctctcctc aagcgtattc aacaaggggc tgaaggatgc ccagaaggta
    13801 ccccattgta tgggatctga tctggggcct cggtacacat gctttacatg tgtttagtcg
    13861 aggttaaaaa aacgtctagg ccccccgaac cacggggacg tggttttcct ttgaaaaaca
    13921 cgatgataat aattcaccat gtctagactg gacaagagca aagtcataaa ctctgctctg
    13981 gaattactca atgaagtcgg tatcgaaggc ctgacgacaa ggaaactcgc tcaaaagctg
    14041 ggagttgagc agcctaccct gtactggcac gtgaagaaca agcgggccct gctcgatgcc
    14101 ctggcaatcg agatgctgga caggcatcat acccacttct gccccctgga aggcgagtca
    14161 tggcaagact ttctgcggaa caacgccaag tcattccgct gtgctctcct ctcacatcgc
    14221 gacggggcta aagtgcatct cggcacccgc ccaacagaga aacagtacga aaccctggaa
    14281 aatcagctcg cgttcctgtg tcagcaaggc ttctccctgg agaacgcact gtacgctctg
    14341 tccgccgtgg gccactttac actgggctgc gtattggagg atcaggagca tcaagtagca
    14401 aaagaggaaa gagagacacc taccaccgat tctatgcccc cacttctgag acaagcaatt
    14461 gagctgttcg accatcaggg agccgaacct gccttccttt tcggcctgga actaatcata
    14521 tgtggcctgg agaaacagct aaagtgcgaa agcggcgggc cggccgacgc ccttgacgat
    14581 tttgacttag acatgctccc agccgatgcc cttgacgact ttgaccttga tatgctgcct
    14641 gctgacgctc ttgacgattt tgaccttgac atgctccccg ggtaactaag taaggatcaa
    14701 atggcgcgtt acgtaaagct tgtcgataat caacctctgg attacaaaat ttgtgaaaga
    14761 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg
    14821 cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc
    14881 tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc
    14941 actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt
    15001 tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt
    15061 gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg
    15121 aaatcatcgt cctttccttg gctgctcgcc tatgttgcca cctggattct gcgcgggacg
    15181 tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg
    15241 ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt
    15301 tgggccgcct ccccgcatcg acaccgagcg ctgctcgagg cctcgactgt gccttctagt
    15361 tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact
    15421 cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat
    15481 tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc
    15541 aggcatgctg gggacacgtg ggatccggtg ccagggcgtg cccttgggct ccccgggcgc
    15601 gtttgggcgg ccgcatcgaa ttctaccggg taggggaggc gcttttccca aggcagtctg
    15661 gagcatgcgc tttagcagcc ccgctgggca cttggcgcta cacaagtggc ctctggcctc
    15721 gcacacattc cacatcctcc ggtaggcgcc aaccggctcc gttctttggt ggccccttcg
    15781 cgccaccttc tactcctccc ctagtcagga agttcccccc cgccccgcag ctcgcgtcgt
    15841 gcaggacgtg acaaatggaa gtagcacgtc tcactagtct cgtgcagatg gacagcaccg
    15901 ctgagcaatg gaagcgggta ggcctttggg gcagcggcca atagcagctt tgctccttcg
    15961 ctttctgggc tcagaggctg ggaaggggtg ggtccggggg cgggctcagg ggcgggctca
    16021 ggggcggggc gggcgcccga aggtcctccg gaggcccggc attctgcacg cttcaaaagc
    16081 gcacgtctgc cgcgctgttc tcctcttcct catctccggg cctttcgacc tgcaatcgcc
    16141 gctagccgcc accatgaaaa agcctgaact caccgcgacg tctgtcgaga agtttctgat
    16201 cgaaaagttc gacagcgtct ccgacctgat gcagctctcg gagggcgaag aatctcgtgc
    16261 tttcagcttc gatgtaggag ggcgtggata tgtcctgcgg gtaaatagct gcgccgatgg
    16321 tttctacaaa gatcgttatg tttatcggca ctttgcatcg gccgcgctcc cgattccgga
    16381 agtgcttgac attggggaat tcagcgagag cctgacctat tgcatctccc gccgtgcaca
    16441 gggtgtcacg ttgcaagacc tgcctgaaac cgaactgccc gctgttctgc agccggtcgc
    16501 ggaggccatg gatgcgatcg ctgcggccga tcttagccag acgagcgggt tcggcccatt
    16561 cggaccgcaa ggaatcggtc aatacactac atggcgtgat ttcatatgcg cgattgctga
    16621 tccccatgtg tatcactggc aaactgtgat ggacgacacc gtcagtgtaa gtgctagccc
    16681 ctaggcctga atcgaaaaac cgctcagcgg cgcccgcacc caaacgaaaa cacccccggc
    16741 ccaagcggac cgggggtgtc gcgtgaaatg gatcagtgag cgtggccgtg gtggtggccg
    16801 tggccgtgct cgtcctcgtc ggccggcttg tcgacgacgg cggtctccgt cgtcaggatc
    16861 atccgggcca ccgaggcggc gttgagaaca gccgagcggg tcaccttggc cggatcgacg
    16921 acgccggcgg agaccagatc accgaactcc agcgtggcgg cgttgaagcc ctgccccttg
    16981 ggaagttcgg agagagctcg tcgactgcag aggcctgcat gcaagcttgg cgtaatcatg
    17041 gtcatagctg tttccttatc agctcactca aaggcggtaa tacggttatc cacagaatca
    17101 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
    17161 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
    17221 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
    17281 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
    17341 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
    17401 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
    17461 cgctgccctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
    17521 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
    17581 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
    17641 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
    17701 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
    17761 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
    17821 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
    17881 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
    17941 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
    18001 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
    18061 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
    18121 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
    18181 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgc
//
