LOCUS       pCV296                  7707 bp ds-DNA     circular     30-MAR-2021
DEFINITION  .
KEYWORDS    "Key Features:YebT(rings1-2-3-4-6-7)-His " "Backbone:pDCE587"      
            "Construction:2 piece gibson cloned into DCE587 digested with BsaI
            " "Resistance:Amp" "Location:Centaurus (-80 C), Comet (-20 C)"
            "Rack" "Box" "Notes" "Sequencing information:y –  11-17-20 -
            pCV296 – 1 -CRVDN2129 CRVDN2130 CRVDN2131 CRVDN2132 " "Special
            Category"
FEATURES             Location/Qualifiers
     CDS             complement(1340..2419)
                     /label="lacI"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     promoter        2806..2822
                     /label="T7 promoter"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer_bind     2806..2825
                     /label="T7"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_binding    2825..2847
                     /label="LacO"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     CDS             2825..2849
                     /label="lacO"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    2903..2905
                     /label="Start"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     CDS             2906..5056
                     /label="MCE domain protein"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     CDS             2906..5056
                     /label="yebT"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     CDS             2906..5056
                     /label="Translation 2906-5056"
     misc_feature    2912..3190
                     /label="MCE domain (CDD)"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    3254..3499
                     /label="MCE domain (weak/partial hit, CDD)"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     primer          3349..3370
                     /label="CVP640"
                     /note="sequence: cgactatgccatcaatcccaac"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    3614..3886
                     /label="MCE domain (CDD)"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     primer          3950..3968
                     /label="CVP641"
                     /note="sequence: gatgttctgacgctgaccc"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    3950..4189
                     /label="MCE domain (weak/partial hit, CDD)"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     STS             4086..4324
                     /label="STS"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    4268..4324
                     /label="end4"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    4325..4362
                     /label="start6"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    4325..4597
                     /label="MCE domain (CDD)"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    4661..4918
                     /label="MCE domain (CDD)"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    4844..4846
                     /label="Changed C -> G for silent mutation"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     CDS             5057..5086
                     /label="Translation 5057-5086"
     misc_feature    5066..5083
                     /label="His Tag"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    5084..5086
                     /label="STOP"
                     /ApEinfo_revcolor=#a300ff
                     /ApEinfo_fwdcolor=#a300ff
     terminator      5164..5210
                     /label="T7 terminator"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     rep_origin      5247..5702
                     /label="f1 origin"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     rep_origin      5247..5702
                     /label="M13 origin"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     rep_origin      5264..5570
                     /label="F1 ori"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    complement(5724..5884)
                     /label="Fragment from pL452"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_recomb     5778..5811
                     /label="LoxP"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     gene            complement(5778..5811)
                     /label="loxP"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     CDS             5995..6710
                     /label="bla"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     CDS             6193..6710
                     /label="AmpR"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     CDS             6712..6852
                     /label="AmpR"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     CDS             6712..6852
                     /label="bla"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     rep_origin      6950..7632
                     /label="ColE1 origin"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
ORIGIN
        1 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
       61 agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat
      121 ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg
      181 catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg
      241 acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta
      301 cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc
      361 gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat
      421 gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct
      481 tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt
      541 gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac
      601 gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact
      661 ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt
      721 taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa
      781 cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa
      841 gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg
      901 ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt
      961 cctcaacgac aggagcacga tcatgcgcac ccgtggggcc gccatgccgg cgataatggc
     1021 ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg
     1081 caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc
     1141 ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa
     1201 gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg
     1261 gttgaaggct ctcaagggca tcggtcgaga tcccggtgcc taatgagtga gctaacttac
     1321 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
     1381 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgcc agggtggttt
     1441 ttcttttcac cagtgagacg ggcaacagct gattgccctt caccgcctgg ccctgagaga
     1501 gttgcagcaa gcggtccacg ctggtttgcc ccagcaggcg aaaatcctgt ttgatggtgg
     1561 ttaacggcgg gatataacat gagctgtctt cggtatcgtc gtatcccact accgagatat
     1621 ccgcaccaac gcgcagcccg gactcggtaa tggcgcgcat tgcgcccagc gccatctgat
     1681 cgttggcaac cagcatcgca gtgggaacga tgccctcatt cagcatttgc atggtttgtt
     1741 gaaaaccgga catggcactc cagtcgcctt cccgttccgc tatcggctga atttgattgc
     1801 gagtgagata tttatgccag ccagccagac gcagacgcgc cgagacagaa cttaatgggc
     1861 ccgctaacag cgcgatttgc tggtgaccca atgcgaccag atgctccacg cccagtcgcg
     1921 taccgtcttc atgggagaaa ataatactgt tgatgggtgt ctggtcagag acatcaagaa
     1981 ataacgccgg aacattagtg caggcagctt ccacagcaat ggcatcctgg tcatccagcg
     2041 gatagttaat gatcagccca ctgacgcgtt gcgcgagaag attgtgcacc gccgctttac
     2101 aggcttcgac gccgcttcgt tctaccatcg acaccaccac gctggcaccc agttgatcgg
     2161 cgcgagattt aatcgccgcg acaatttgcg acggcgcgtg cagggccaga ctggaggtgg
     2221 caacgccaat cagcaacgac tgtttgcccg ccagttgttg tgccacgcgg ttgggaatgt
     2281 aattcagctc cgccatcgcc gcttccactt tttcccgcgt tttcgcagaa acgtggctgg
     2341 cctggttcac cacgcgggaa acggtctgat aagagacacc ggcatactct gcgacatcgt
     2401 ataacgttac tggtttcaca ttcaccaccc tgaattgact ctcttccggg cgctatcatg
     2461 ccataccgcg aaaggttttg cgccattcga tggtgtccgg gatctcgacg ctctccctta
     2521 tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc
     2581 gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc
     2641 accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca
     2701 tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc
     2761 acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaattaata cgactcacta
     2821 taggggaatt gtgagcggat aacaattccc ctctagaaat aattttgttt aactttGGCC
     2881 CAGGCGGCCa ggagGGTCTC acATGcagga ccggggtaat accgtcacca tcgactttat
     2941 gtcggcggat ggtattgttc caggccgtac gcctgttcgt tatcagggcg ttgaagtcgg
     3001 aacagtgcag gatatcagcc tcagcgacga tcttcgtaag attgaagtca aggtcagcat
     3061 caagtccgat atgaaagatg cgctgcgcga agagactcag ttctggctgg tgacgccaaa
     3121 agcatcgttg gcaggtgtct ccgggctgga cgccctcgtc ggtgggaact atatcggcat
     3181 gatgccgggt aaaggtaaag agcaggatca ctttgtcgca ctcgataccc aaccgaaata
     3241 tcggctggac aatggcgatc tgatgatcca cctgcaagcc cccgatctcg gttcgctgaa
     3301 cagcggttca ttggtctatt tccgcaagat cccggtggga aaagtctacg actatgccat
     3361 caatcccaac aagcaaggcg tggtgattga tgtcctgatc gagcggcgtt ttaccgatct
     3421 ggtgaaaaaa ggtagccgtt tctggaacgt ttccggcgtt gatgccaacg tcagtatcag
     3481 tggcgcgaag gtgaaactgg aaagcctggc ggcactggtt aacggtgcga ttgccttcga
     3541 ttcaccagaa gagtcgaaac ctgccgaggc ggaagatacc tttggtctgt atgaagatct
     3601 ggcccacagc cagcgtggcg taataataaa actggaactg ccgagtgggg ccggattaac
     3661 cgccgactcg acgccgttaa tgtatcaggg gctggaagtc ggacagctga ctaaactgga
     3721 tttaaatcct ggtggtaaag tcacagggga aatgaccgtt gatcccagcg tcgttaccct
     3781 gcttcgtgaa aatacccgca tcgaattacg caacccgaaa ttatccctta gcgatgctaa
     3841 tctcagcgcc ctgctgaccg gcaaaacctt cgagctggtg cccggcgatg gcgagccacg
     3901 caaagagttc gttgttgtgc caggcgaaaa agcactgctg catgaacctg atgttctgac
     3961 gctgaccctg accgcgccgg aaagttacgg tattgatgcg ggtcagccgc tcattcttca
     4021 cggcgtgcag gtaggccagg ttatcgatcg taaactcacc agcaaaggcg tcacctttac
     4081 cgtcgccatc gagcctcagc atcgcgaact ggtaaaaggc gatagcaaat ttgtcgtcaa
     4141 cagccgtgtc gatgtgaagg tggggctgga tggcgttgag tttctcggtg ccagcgcctc
     4201 agaatggatc aatggcggga tacgtattct gccgggcgat aaaggtgaga tgaaagccag
     4261 ctatccactg tatgccaatt tggaaaaagc gctggagaac agccttagcg atttacccac
     4321 cacaggcggg cagattacgc ttcacgcttt cgatgccgga aaactggcgg tcgggatgcc
     4381 aattcgctat ctcggtattg atatcgggca aatccagacg ctggatctga ttaccgcacg
     4441 caatgaagta caggcaaagg cggtacttta cccggaatat gtccagacct ttgctcgcgg
     4501 tggtacgcgc ttctcagtgg tcacaccgca aatttcggca gctggcgttg agcatcttga
     4561 tactatcctc cagccgtata tcaacgtcga accaggccgg ggcaatcctc gccgcgactt
     4621 tgaattacaa gaggccacca ttactgattc gcgttacctg gatggcttaa gcattattgt
     4681 tgaagcgccg gaagccggtt cgttaggcat cggtacgcct gtgctgttcc gtggtctgga
     4741 agtcggtacg gttacaggaa tgacgctggg gacattgtca gatcgcgtga tgattgcgat
     4801 gcgcatcagt aaacgctatc aacacctggt gcgtaacaat tccgtGttct ggttggcatc
     4861 gggttacagt ctggactttg gtctgacggg cggcgtagtg aaaaccggca cctttaacca
     4921 gtttatccgt ggcggcatcg ccttcgccac gcctccgggt acgccactgg caccgaaagc
     4981 ccaggaaggc aaacacttcc tgttgcagga aagtgaaccg aaagagtggc gtgaatgggg
     5041 aactgcgctt cccaaaggcg agaccCATCA CCACCATCAC CATtaaGGCC AGGCCGGCCg
     5101 atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat
     5161 aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag
     5221 gaactatatc cggattggcg aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg
     5281 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
     5341 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
     5401 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
     5461 ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
     5521 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc
     5581 tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa
     5641 aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa cgtttacaat
     5701 ttcaggtggc acttttcggg gaaCGACGAC CTGCAGCCAA GCTAGCTTGG CTGGACGTAA
     5761 ACTCCTCTTC AGACCTAATA ACTTCGTATA GCATACATTA TACGAAGTTA TATTAAGGGT
     5821 TATTGAATAT GATCGGAATT GGGCTGCAGG AATTCGATAG CTTGGCTGCA GGTCGACCTC
     5881 GAGGatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
     5941 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
     6001 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
     6061 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
     6121 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
     6181 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt
     6241 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
     6301 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
     6361 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
     6421 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
     6481 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca
     6541 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
     6601 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
     6661 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg ctctcgcggt
     6721 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
     6781 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
     6841 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
     6901 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     6961 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     7021 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     7081 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     7141 ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac
     7201 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     7261 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     7321 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     7381 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     7441 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     7501 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     7561 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     7621 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     7681 cctgcgttat cccctgattc tgtggat
//