LOCUS       Exported                9186 bp DNA     circular SYN 16-SEP-2021
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9186)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 11, 2022 from SnapGene 6.0.0
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9186
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
                     /note="color: #ffffff"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
                     /note="color: #ffffff; direction: RIGHT"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /note="color: #ffffff; direction: RIGHT"
     regulatory      901..910
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /note="color: #a6acb3"
                     /regulatory_class="other"
     CDS             922..939
                     /codon_start=1
                     /label=6His
                     /note="color: #008000"
                     /translation="HHHHHH"
     CDS             943..975
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /note="color: #cc99b2"
                     /translation="MASMTGGQQMG"
     CDS             979..1002
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /note="This feature has 2 segments:
                        1:  979 ..  987 / #cc99b2
                        2:  988 .. 1002 / #cc99b2 / enterokinase site
                     Cleavage site after base 1002"
                     /translation="DLYDDDDK"
     CDS             1009..1725
                     /codon_start=1
                     /product="enhanced CFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mECFP
                     /note="mammalian codon-optimized"
                     /note="This feature has 3 segments:
                        1: 1009 .. 1011 / #00ffff
                        2: 1012 .. 1014 / #00ffff / 1a
                        3: 1015 .. 1725 / #00ffff"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHRFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     AHFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     CDS             1735..3933
                     /codon_start=1
                     /label=Epac1 (149-881,Q270E)
                     /note="color: #ff7e79"
                     /translation="PVGTHEMEEELAEAVALLSQRGPDALLTVALRKPPGQRTDEELDL
                     IFEELLHIKAVAHLSNSVKRELAAVLLFEPHSKAGTVLFSQGDKGTSWYIIWKGSVNVV
                     THGKGLVTTLHEGDDFGELALVNDAPRAATIILREDNCHFLRVDKQDFNRIIKDVEAKT
                     MRLEEHGKVVLVLERASQGAGPSRPPTPGRNRYTVMSGTPEKILELLLEAMGPDSSAHD
                     QTETFLSDFLLTHRVFMPSAQLCAALLHHFHVEPAGGSEQERSTYVCNKRQQILRLVSQ
                     WVALYGSMLHTDPVATSFLQKLSDLVGRDTRLSNLLREQWPERRRCHRLENGCGNASPQ
                     MKARNLPVWLPNQDEPLPGSSCAIQVGDKVPYDICRPDHSVLTLQLPVTASVREVMAAL
                     AQEDGWTKGQVLVKVNSAGDAIGLQPDARGVATSLGLNERLFVVNPQEAHELIPHPDQL
                     GPTVGSAEGLDLVSAKDLAGQLTDHDWSLFNSIHQVELIHYVLGPQHLRDVTTANLERF
                     MRRFNELQYWVATELCLCPVPGPRAQLLRKFIKLAAHLKEQKNLNSFFAVMFGLSNSAI
                     SHLAHTWERLPHKVRKLYSALERLLDPSWNHRVYRLALAKLSPPVIPFMPLLLKDMTFI
                     HEGNHTLVENLINFEKMRMMARVARMLHHCRSHNPVPLSPLRSRVSHLHEDSQVARIST
                     CSEQSLSTRSPASTWAYVQQLKVIDNQRELSRLSRELEP"
     CDS             3940..4674
                     /codon_start=1
                     /label=cpVenus(L194)
                     /note="color: #fffb00"
                     /translation="MPDNHYLSYQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYKG
                     GTGGSMVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKLICTTGKL
                     PVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEV
                     KFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKANFKIRHNI
                     EDGGVQLADHYQQNTPIGDGPVL"
     promoter        complement(4738..4756)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
                     /note="color: #ffffff; direction: LEFT"
     polyA_signal    4782..5006
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
                     /note="color: #a6acb3"
     rep_origin      5052..5480
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
     promoter        5494..5824
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
                     /note="color: #ffffff; direction: RIGHT"
     rep_origin      5675..5810
                     /label=SV40 ori
                     /note="SV40 origin of replication"
                     /note="color: #ffff00"
     CDS             5891..6685
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /note="color: #ccffcc"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    6859..6980
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
                     /note="color: #a6acb3"
     primer_bind     complement(7029..7045)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: LEFT"
     protein_bind    7053..7069
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #31849b"
     promoter        complement(7077..7107)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
                     /note="This reverse directional feature has 3 segments:
                        1: 7077 .. 7083 / #ffffff / -10
                        2: 7084 .. 7101 / #ffffff
                        3: 7102 .. 7107 / #ffffff / -35"
     protein_bind    7122..7143
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
                     /note="color: #31849b"
     rep_origin      complement(7431..8019)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     CDS             complement(8190..9050)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 8190 .. 8981 / #ccffcc
                        2: 8982 .. 9050 / #ccffcc / signal sequence
                     Cleavage site after base 8981"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(9051..9155)
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: LEFT"
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttgcggcc
      901 gccaccatgg tgcggggttc tcatcatcat catcatcatg gtatggctag catgactggt
      961 ggacagcaaa tgggtcggga tctgtacgac gatgacgata aggatcccat ggtgagcaag
     1021 ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac
     1081 ggccacaggt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc
     1141 ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc
     1201 ctgacctggg gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc
     1261 ttcaagtccg ccatgcccga aggctacgtc caggagcgta ccatcttctt caaggacgac
     1321 ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc
     1381 gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac
     1441 aactacatca gccacaacgt ctatatcacc gccgacaagc agaagaacgg catcaaggcc
     1501 cacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag
     1561 cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc
     1621 cagtccaagc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc
     1681 gtgaccgccg ccgggatcac tcacggcatg gacgagctgt acaaggctgg tacccccgtg
     1741 ggaactcatg agatggagga ggagttggcc gaagctgtgg ccctgctctc ccagcggggg
     1801 cctgacgccc tgctcactgt ggcacttcga aagcccccag gtcagcgcac ggatgaagag
     1861 ctggacctca tctttgagga gctgctgcac atcaaggctg tggcccacct ctccaactcg
     1921 gtgaagcgag aattagcggc tgttctgctc tttgaaccac acagcaaggc agggaccgtg
     1981 ttgttcagcc agggggacaa gggcacttcg tggtacatta tctggaaggg atctgtcaac
     2041 gtggtgaccc atggcaaggg gctggtgacc accctgcatg agggagatga ttttggagaa
     2101 ctggctctgg tgaatgatgc accccgggca gccaccatca tcctgcgaga agacaactgt
     2161 catttcctgc gtgtggacaa gcaggacttc aaccgtatca tcaaggatgt ggaggcaaag
     2221 accatgcggc tggaagaaca tggcaaagtg gtgctggtgc tggagagagc ctctcagggc
     2281 gccggccctt cccgaccccc aaccccaggc aggaaccggt atacagtgat gtctggcacc
     2341 ccagagaaga tcctagagct tctgttggag gccatgggac cagattccag tgctcatgac
     2401 caaacagaga cattcctcag cgacttcctc ctgacccaca gggtcttcat gcccagcgcc
     2461 caactctgcg ctgcccttct gcaccacttc catgtggagc ctgcgggtgg cagcgagcag
     2521 gagcgcagca cctacgtctg caacaagagg cagcagatct tgcggctggt cagccagtgg
     2581 gtggccctgt atggctccat gctccacact gaccctgtgg ccaccagctt cctccagaaa
     2641 ctctcagacc tggtgggcag ggacacccga ctcagcaacc tgctgaggga gcagtggcca
     2701 gagaggcggc gatgccacag gttggagaat ggctgtggga atgcatctcc tcagatgaag
     2761 gcccggaact tgcctgtttg gctccccaac caggacgagc cccttcctgg cagcagctgt
     2821 gccatccaag ttggggataa agtcccctat gacatctgcc ggccagacca ctcagtgttg
     2881 accctgcagc tgcctgtgac agcctccgtg agagaggtga tggcagcgtt ggcccaggag
     2941 gatggctgga ccaaggggca ggtgctggtg aaggtcaatt ctgcaggtga tgccattggc
     3001 ctgcagccag atgcccgtgg tgtggccaca tctctggggc tcaatgagcg tctctttgtt
     3061 gtcaacccac aggaagcgca tgagctgatc ccacaccctg accagctggg gcccactgtg
     3121 ggctctgctg aggggctgga cctggtgagt gccaaggacc tggcaggcca gctgacggac
     3181 cacgactgga gcctcttcaa cagtatccac caggtggagc tgatccacta tgtgctgggc
     3241 ccccagcatc tgcgggatgt caccaccgcc aacctggagc gcttcatgcg ccgcttcaat
     3301 gagctgcagt actgggtggc caccgagctg tgtctctgcc ccgtgcccgg cccccgggcc
     3361 cagctgctca ggaagttcat taagctggcg gcccacctca aggagcagaa gaatctcaat
     3421 tccttctttg ccgtcatgtt tggcctcagc aactcggcca tcagccacct agcccacacc
     3481 tgggagcggc tgccccacaa agtccggaag ctgtactccg ccctcgagag gctgctggac
     3541 ccctcatgga accaccgggt ataccgactg gccctcgcca agctctcccc tcctgtcatc
     3601 cccttcatgc cccttcttct caaagacatg accttcattc atgagggaaa ccacacacta
     3661 gtggagaatc tcatcaactt tgagaagatg agaatgatgg ccagagtcgc gcggatgctg
     3721 caccactgcc gaagccacaa ccctgtgcct ctctcaccac tcagaagccg agtttcccac
     3781 ctccacgagg acagccaggt ggcgaggatt tccacatgct cggagcagtc cctgagcacc
     3841 cggagtccag ccagcacctg ggcttatgtc cagcagctga aggtcattga caaccagcgg
     3901 gaactctccc gcctctcccg agagctggag ccagagctca tgcccgacaa ccactacctg
     3961 agctaccagt ccaagctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg
     4021 gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa gggaggtacc
     4081 ggtggatcta tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc
     4141 gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat
     4201 gccacctacg gcaagctgac cctgaagctg atctgcacca ccggcaagct gcccgtgccc
     4261 tggcccaccc tcgtgaccac cctgggctac ggccttcagt gcttcgcccg ctaccccgac
     4321 cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc
     4381 accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc
     4441 gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc
     4501 ctggggcaca agctggagta caactacaac agccacaacg tctatatcac cgccgacaag
     4561 cagaagaacg gcatcaaggc caacttcaag atccgccaca acatcgagga cggcggcgtg
     4621 cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt gctgtaagaa
     4681 ttctgcagat atccatcaca ctggcggccg ctcgagcatg catctagagg gccctattct
     4741 atagtgtcac ctaaatgcta gagctcgctg atcagcctcg actgtgcctt ctagttgcca
     4801 gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac
     4861 tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat
     4921 tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca
     4981 tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctctag
     5041 ggggtatccc cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
     5101 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
     5161 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcggggca tccctttagg
     5221 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
     5281 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
     5341 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
     5401 ttttgattta taagggattt tggggatttc ggcctattgg ttaaaaaatg agctgattta
     5461 acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc
     5521 ccaggctccc caggcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag
     5581 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
     5641 gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
     5701 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     5761 ctctgcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg
     5821 caaaaagctc ccgggagctt gtatatccat tttcggatct gatcaagaga caggatgagg
     5881 atcgtttcgc atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga
     5941 gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt
     6001 ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct
     6061 gaatgaactg caggacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg
     6121 cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt
     6181 gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc
     6241 tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc
     6301 gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga
     6361 tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg
     6421 catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat
     6481 ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg
     6541 ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc
     6601 tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta
     6661 tcgccttctt gacgagttct tctgagcggg actctggggt tcgaaatgac cgaccaagcg
     6721 acgcccaacc tgccatcacg agatttcgat tccaccgccg ccttctatga aaggttgggc
     6781 ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg
     6841 gagttcttcg cccaccccaa cttgtttatt gcagcttata atggttacaa ataaagcaat
     6901 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
     6961 aaactcatca atgtatctta tcatgtctgt ataccgtcga cctctagcta gagcttggcg
     7021 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
     7081 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
     7141 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
     7201 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
     7261 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     7321 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     7381 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     7441 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     7501 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     7561 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     7621 tctcaatgct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     7681 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     7741 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     7801 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     7861 tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     7921 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     7981 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     8041 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     8101 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     8161 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     8221 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     8281 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     8341 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     8401 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     8461 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     8521 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     8581 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     8641 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     8701 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     8761 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     8821 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     8881 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     8941 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     9001 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     9061 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     9121 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     9181 gacgtc
//
