LOCUS       Exported                8992 bp DNA     circular SYN 29-OCT-2021
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8992)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 12, 2022 from SnapGene 6.0.0
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8992
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        105..484
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
                     /note="color: #ffffff"
     promoter        485..688
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
                     /note="color: #ffffff; direction: RIGHT"
     promoter        733..751
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /note="color: #ffffff; direction: RIGHT"
     CDS             789..1499
                     /codon_start=1
                     /label=mRuby2
                     /note="color: #fb0106"
                     /translation="MVSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGNPYMGTQTM
                     RITVIEGGPLPFAFDILATSFMYGSRTFIKYPKGIPDFFKQSFPEGFTWERVTRYEDGG
                     VVTVMQDTSLEDGCLVYHVQVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTH
                     MALKVDGGGHLSCSFVTTYRSKKTVGNIKMPGIHAVDHRLERLEESDNEMFVVQREVAV
                     AKFAGLGGGMDELYK"
     CDS             1509..3704
                     /codon_start=1
                     /label=Epac1 (149-880; Q270E)
                     /note="color: #ff7e79"
                     /translation="PVGTHEMEEELAEAVALLSQRGPDALLTVALRKPPGQRTDEELDL
                     IFEELLHIKAVAHLSNSVKRELAAVLLFEPHSKAGTVLFSQGDKGTSWYIIWKGSVNVV
                     THGKGLVTTLHEGDDFGELALVNDAPRAATIILREDNCHFLRVDKQDFNRIIKDVEAKT
                     MRLEEHGKVVLVLERASQGAGPSRPPTPGRNRYTVMSGTPEKILELLLEAMGPDSSAHD
                     QTETFLSDFLLTHRVFMPSAQLCAALLHHFHVEPAGGSEQERSTYVCNKRQQILRLVSQ
                     WVALYGSMLHTDPVATSFLQKLSDLVGRDTRLSNLLREQWPERRRCHRLENGCGNASPQ
                     MKARNLPVWLPNQDEPLPGSSCAIQVGDKVPYDICRPDHSVLTLQLPVTASVREVMAAL
                     AQEDGWTKGQVLVKVNSAGDAIGLQPDARGVATSLGLNERLFVVNPQEAHELIPHPDQL
                     GPTVGSAEGLDLVSAKDLAGQLTDHDWSLFNSIHQVELIHYVLGPQHLRDVTTANLERF
                     MRRFNELQYWVATELCLCPVPGPRAQLLRKFIKLAAHLKEQKNLNSFFAVMFGLSNSAI
                     SRLAHTWERLPHKVRKLYSALERLLDPSWNHRVYRLALAKLSPPVIPFMPLLLKDMTFI
                     HEGNHTLVENLINFEKMRMMARAARMLHHCRSHNPVPLSPLRSRVSHLHEDSQVARIST
                     CSEQSLSTRSPASTWAYVQQLKVIDNQRELSRLSRELE"
     CDS             3705..4418
                     /codon_start=1
                     /label=sfGFP
                     /note="color: #008f00"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATIGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     KYKTRAVVKFEGDTLVNRIELKGTDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFTVRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQTVLSKDPNEKRDHMVLHE
                     YVNAAGITHGMDELYK"
     polyA_signal    4459..4683
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
                     /note="color: #a6acb3"
     rep_origin      4729..5157
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
     promoter        5171..5500
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
                     /note="color: #ffffff; direction: RIGHT"
     rep_origin      5351..5486
                     /label=SV40 ori
                     /note="SV40 origin of replication"
                     /note="color: #ffff00"
     CDS             5567..6361
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /note="color: #ccffcc"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    6535..6656
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
                     /note="color: #a6acb3"
     primer_bind     complement(6705..6721)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: LEFT"
     protein_bind    6729..6745
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #31849b"
     promoter        complement(6753..6783)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
                     /note="This reverse directional feature has 3 segments:
                        1: 6753 .. 6759 / #ffffff / -10
                        2: 6760 .. 6777 / #ffffff
                        3: 6778 .. 6783 / #ffffff / -35"
     protein_bind    6798..6819
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
                     /note="color: #31849b"
     rep_origin      complement(7107..7695)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     CDS             complement(7866..8726)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 7866 .. 8657 / #ccffcc
                        2: 8658 .. 8726 / #ccffcc / signal sequence
                     Cleavage site after base 8657"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(8727..8831)
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: LEFT"
ORIGIN
        1 ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc ttagggttag
       61 gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt gattattgac
      121 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
      181 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      241 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      301 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      361 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      421 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      481 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      541 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      601 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      661 acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca ctgcttactg
      721 gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc gtttaaactt
      781 aagcttggat ggtgtctaag ggcgaagagc tgatcaagga aaatatgcgt atgaaggtgg
      841 tcatggaagg ttcggtcaac ggccaccaat tcaaatgcac aggtgaagga gaaggcaatc
      901 cgtacatggg aactcaaacc atgaggatca cagtcatcga gggaggaccc ctgccatttg
      961 cctttgacat tcttgccacg tcgttcatgt atggcagccg tacttttatc aagtacccga
     1021 aaggcattcc tgatttcttt aaacagtcct ttcctgaggg ttttacttgg gaaagagtta
     1081 cgagatacga agatggtgga gtcgtcaccg tcatgcagga caccagcctt gaggatggct
     1141 gtctcgttta ccacgtccaa gtcagagggg taaactttcc ctccaatggt cccgtgatgc
     1201 agaagaagac caagggttgg gagcctaata cagagatgat gtatccagca gatggtggtc
     1261 tgaggggata cactcatatg gcactgaaag ttgatggtgg tggccatctg tcttgctctt
     1321 tcgtaacaac ttacaggtca aaaaagaccg tcgggaacat caagatgccc ggtatccatg
     1381 ccgttgatca ccgcctggaa aggttagagg aaagtgacaa tgaaatgttc gtagtacaac
     1441 gcgaagtcgc agttgccaag ttcgccgggc ttggtggtgg gatggacgag ctgtacaagg
     1501 ctggtacccc cgtgggaact catgagatgg aggaggagtt ggccgaagct gtggccctgc
     1561 tctcccagcg ggggcctgac gccctgctca ctgtggcact tcgaaagccc ccaggtcagc
     1621 gcacggatga agagctggac ctcatctttg aggagctgct gcacatcaag gctgtggccc
     1681 acctctccaa ctcggtgaag cgagaattag cggctgttct gctctttgaa ccacacagca
     1741 aggcagggac cgtgttgttc agccaggggg acaagggcac ttcgtggtac attatctgga
     1801 agggatctgt caacgtggtg acccatggca aggggctggt gaccaccctg catgagggag
     1861 atgattttgg agaactggct ctggtgaatg atgcaccccg ggcagccacc atcatcctgc
     1921 gagaagacaa ctgtcatttc ctgcgtgtgg acaagcagga cttcaaccgt atcatcaagg
     1981 atgtggaggc aaagaccatg cggctggaag aacatggcaa agtggtgctg gtgctggaga
     2041 gagcctctca gggcgccggc ccttcccgac ccccaacccc aggcaggaac cggtatacag
     2101 tgatgtctgg caccccagag aagatcctag agcttctgtt ggaggccatg ggaccagatt
     2161 ccagtgctca tgaccaaaca gagacattcc tcagcgactt cctcctgacc cacagggtct
     2221 tcatgcccag cgcccaactc tgcgctgccc ttctgcacca cttccatgtg gagcctgcgg
     2281 gtggcagcga gcaggagcgc agcacctacg tctgcaacaa gaggcagcag atcttgcggc
     2341 tggtcagcca gtgggtggcc ctgtatggct ccatgctcca cactgaccct gtggccacca
     2401 gcttcctcca gaaactctca gacctggtgg gcagggacac ccgactcagc aacctgctga
     2461 gggagcagtg gccagagagg cggcgatgcc acaggttgga gaatggctgt gggaatgcat
     2521 ctcctcagat gaaggcccgg aacttgcctg tttggctccc caaccaggac gagccccttc
     2581 ctggcagcag ctgtgccatc caagttgggg ataaagtccc ctatgacatc tgccggccag
     2641 accactcagt gttgaccctg cagctgcctg tgacagcctc cgtgagagag gtgatggcag
     2701 cgttggccca ggaggatggc tggaccaagg ggcaggtgct ggtgaaggtc aattctgcag
     2761 gtgatgccat tggcctgcag ccagatgccc gtggtgtggc cacatctctg gggctcaatg
     2821 agcgtctctt tgttgtcaac ccacaggaag cgcatgagct gatcccacac cctgaccagc
     2881 tggggcccac tgtgggctct gctgaggggc tggacctggt gagtgccaag gacctggcag
     2941 gccagctgac ggaccacgac tggagcctct tcaacagtat ccaccaggtg gagctgatcc
     3001 actatgtgct gggcccccag catctgcggg atgtcaccac cgccaacctg gagcgcttca
     3061 tgcgccgctt caatgagctg cagtactggg tggccaccga gctgtgtctc tgccccgtgc
     3121 ccggcccccg ggcccagctg ctcaggaagt tcattaagct ggcggcccac ctcaaggagc
     3181 agaagaatct caattccttc tttgccgtca tgtttggcct cagcaactcg gccatcagcc
     3241 gcctagccca cacctgggag cggctgcccc acaaagtccg gaagctgtac tccgccctcg
     3301 agaggctgct ggacccctca tggaaccacc gggtataccg actggccctc gccaagctct
     3361 cccctcctgt catccccttc atgccccttc ttctcaaaga catgaccttc attcatgagg
     3421 gaaaccacac actagtggag aatctcatca actttgagaa gatgagaatg atggccagag
     3481 ccgcgcggat gctgcaccac tgccgaagcc acaaccctgt gcctctctca ccactcagaa
     3541 gccgagtttc ccacctccac gaggacagcc aggtggcgag gatttccaca tgctcggagc
     3601 agtccctgag cacccggagt ccagccagca cctgggctta tgtccagcag ctgaaggtca
     3661 ttgacaacca gcgggaactc tcccgcctct cccgagagct ggagatgtcc aaaggagaag
     3721 aactgtttac cggtgttgtg ccaattttgg ttgaactcga tggtgatgtc aacggacata
     3781 agttctcagt gagaggcgaa ggagaaggtg acgccaccat tggaaaattg actcttaaat
     3841 tcatctgtac tactggtaaa cttcctgtac catggccgac tctcgtaaca acgcttacgt
     3901 acggagttca gtgcttttcg agatacccag accatatgaa aagacatgac ttttttaagt
     3961 cggctatgcc tgaaggttac gtgcaagaaa gaacaatttc gttcaaagat gatggaaaat
     4021 ataaaactag agcagttgtt aaatttgaag gagatacttt ggttaaccgc attgaactga
     4081 aaggaacaga ttttaaagaa gatggtaata ttcttggaca caaactcgaa tacaatttta
     4141 atagtcataa cgtatacatc actgctgata agcaaaagaa cggaattaaa gcgaatttca
     4201 cagtacgcca taatgtagaa gatggcagtg ttcaacttgc cgaccattac caacaaaaca
     4261 cccctattgg agacggtccg gtacttcttc ctgataatca ctacctctca acacaaacag
     4321 tcctgagcaa agatccaaat gaaaaacgtg accacatggt ccttcatgag tatgtaaatg
     4381 ctgctgggat tacacatggc atggatgagc tctacaaatg atctagaggg cccgtttaaa
     4441 cccgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc
     4501 ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg
     4561 aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg
     4621 acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta
     4681 tggcttctga ggcggaaaga accagctggg gctctagggg gtatccccac gcgccctgta
     4741 gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca
     4801 gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct
     4861 ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc
     4921 acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat
     4981 agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc
     5041 aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc
     5101 cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaattaat
     5161 tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag
     5221 tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc
     5281 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct
     5341 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
     5401 actaattttt tttatttatg cagaggccga ggccgcctct gcctctgagc tattccagaa
     5461 gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctcccgg gagcttatat
     5521 atccattttc ggatctgatc aagagacagg atgaggatcg tttcgcatga ttgaacaaga
     5581 tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc
     5641 acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc
     5701 ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcagg acgaggcagc
     5761 gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac
     5821 tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc
     5881 tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac
     5941 gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg
     6001 tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct
     6061 cgcgccagcc gaactgttcg ccaggctcaa ggcgcgcatg cccgacggcg aggatctcgt
     6121 cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg
     6181 attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac
     6241 ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg
     6301 tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg
     6361 agcgggactc tggggttcga aatgaccgac caagcgacgc ccaacctgcc atcacgagat
     6421 ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc
     6481 ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca ccccaacttg
     6541 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
     6601 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
     6661 gtctgtatac cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct
     6721 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
     6781 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
     6841 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
     6901 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     6961 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     7021 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     7081 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     7141 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     7201 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     7261 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     7321 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     7381 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     7441 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     7501 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
     7561 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     7621 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     7681 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     7741 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     7801 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     7861 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     7921 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     7981 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     8041 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     8101 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     8161 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     8221 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     8281 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     8341 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     8401 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     8461 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     8521 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     8581 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     8641 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     8701 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     8761 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     8821 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc gggagatctc
     8881 ccgatcccct atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt
     8941 atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg cgcgagcaaa at
//
