LOCUS       Exported                7809 bp DNA     circular SYN 13-JAN-2022
DEFINITION  pAAV-MCS from stratagen.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7809)
  AUTHORS   Demo User
  TITLE     Direct Submission
  JOURNAL   Exported Jan 13, 2022 from SnapGene Viewer 6.0.0
            https://www.snapgene.com
COMMENT     Createdd by Wei.  
            
            Storage Type: Basic
            Original Author: 
            UNKNOWN
            mCherry were PCR cloned as NheI-EcoRI-ATG-mCherry-TAG-HindIII from 
            pACK-GCaMP2.0-NLS-mCherry from Eckehard Freitag248|mCherry cds were 
            obtained from MPI-Munich.248|http://www.informaxinc.com/
FEATURES             Location/Qualifiers
     source          1..7809
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..141
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
                     /note="color: #ffe4c4"
     promoter        158..1447
                     /label=CaMKII promoter
                     /note="promoter for the mouse calcium/calmodulin-dependent 
                     kinase II alpha subunit"
                     /note="color: #ffffff; direction: RIGHT"
     protein_bind    1161..1178
                     /label=ACK-F2 Primer
                     /note="ACK-F2 primer binding site"
                     /note="color: #31849b"
     regulatory      1458..1467
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /note="color: #a6acb3"
                     /regulatory_class="other"
     CDS             1460..2509
                     /codon_start=1
                     /label=MCU
                     /note="color: #708090"
                     /translation="MAAAAGRSLLLLLCSRGGGGGAGGCGALTAGCFPGLGVSRHRPHQ
                     QHRTAHQRPASWQSVGAAYCSTVVPSDDVTVVYQNGLPVISVRLPSRRERCQFTLKPIS
                     DSVGVFLRQLQEEDRGIDRVAIYSPDGVRVAASTGIDLLLLDDFKLVINDLTYHVRPPK
                     RDLLSHEDAATLNDVKTLVQQLYTTLCIEQHQLNKERELVERLEDLKQQLAPLEKVRIE
                     ISRKAEKRTTLVLWGGLAYMATQFGILARLTWWEYSWDIMEPVTYFITYGSAMAMYAYF
                     VMTRQEYVYPEARDRQYLLFFHKGAKKSRFDLEKYNQLKDAIAQAEMDLKRLRDPLQVH
                     LPLRQIGEKE"
     CDS             2516..3913
                     /codon_start=1
                     /label=tDimer
                     /note="color: #ff0000"
                     /translation="TMVASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQT
                     AKLKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDG
                     GVVTVTQDSSLQDGTLIYKVKFRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEI
                     HQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGR
                     HHLFLGHGTGSTGSGSSGTASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGTLIYKVKFRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKG
                     EIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSE
                     GRHHLFL"
     regulatory      2517..2526
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /note="color: #a6acb3"
                     /regulatory_class="other"
     misc_feature    3938..4526
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
                     /note="color: #ffffff"
     primer_bind     4024..4042
                     /label=CAAV-R
                     /note="CAAV-R primer"
                     /note="color: #a020f0; direction: RIGHT"
     CDS             complement(4409..4420)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /note="color: #cc99b2
                     Cleavage site after base 4408"
                     /translation="IEGR"
     primer_bind     complement(4529..4545)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: LEFT"
     polyA_signal    4556..5032
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
                     /note="color: #a6acb3"
     repeat_region   5072..5212
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
                     /note="color: #ffe4c4"
     rep_origin      5287..5742
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
     promoter        6024..6128
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: RIGHT"
     CDS             6129..6989
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 6129 .. 6197 / #ccffcc / signal sequence
                        2: 6198 .. 6989 / #ccffcc
                     Cleavage site after base 6197"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      7160..7748
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtaacat tatggcctta ggtcacttca
      181 tctccatggg gttcttcttc tgattttcta gaaaatgaga tgggggtgca gagagcttcc
      241 tcagtgacct gcccagggtc acatcagaaa tgtcagagct agaacttgaa ctcagattac
      301 taatcttaaa ttccatgcct tgggggcatg caagtacgat atacagaagg agtgaactca
      361 ttagggcaga tgaccaatga gtttaggaaa gaagagtcca gggcagggta catctacacc
      421 acccgcccag ccctgggtga gtccagccac gttcacctca ttatagttgc ctctctccag
      481 tcctaccttg acgggaagca caagcagaaa ctgggacagg agccccagga gaccaaatct
      541 tcatggtccc tctgggagga tgggtgggga gagctgtggc agaggcctca ggaggggccc
      601 tgctgctcag tggtgacaga taggggtgag aaagcagaca gagtcattcc gtcagcattc
      661 tgggtctgtt tggtacttct tctcacgcta aggtggcggt gtgatatgca caatggctaa
      721 aaagcaggga gagctggaaa gaaacaagga cagagacaga ggccaagtca accagaccaa
      781 ttcccagagg aagcaaagaa accattacag agactacaag ggggaaggga aggagagatg
      841 aattagcttc ccctgtaaac cttagaaccc agctgttgcc agggcaacgg ggcaatacct
      901 gtctcttcag aggagatgaa gttgccaggg taactacatc ctgtctttct caaggaccat
      961 cccagaatgt ggcacccact agccgttacc atagcaactg cctctttgcc ccacttaatc
     1021 ccatcccgtc tgttaaaagg gccctatagt tggaggtggg ggaggtagga agagcgatga
     1081 tcacttgtgg actaagtttg ttcacatccc cttctccaac cccctcagta catcaccctg
     1141 ggagaacaag gtccacttgc ttctgggccc acacagtcct gcagtattgt gtatataagg
     1201 ccagggcaac ggaggagcag gttttgaagt gaaaggcagg caggtgttgg ggaggcagtt
     1261 accggggcaa cgggaacagg gcgtttcgga ggtggttgcc atggggacct ggatgctgac
     1321 gaaggctcgc gaggctgtga gcagccacag tgccctgctc agaagcccca agctcgtcaa
     1381 tcaagctggt tctccatttg cactcaggag cacgggcagg cgagtggccc ctagttctgg
     1441 gggcagcggg ggatccacca tggcggccgc cgcaggtaga tcgctcctgc tgctgctctg
     1501 ctcccggggc ggcggcgggg gcgccggcgg ctgcggagcg ctgactgccg gctgctttcc
     1561 cgggctgggc gtcagccgcc accggccgca ccagcagcac cggacggcac accagaggcc
     1621 ggcttcctgg cagagcgtgg gagccgcata ttgcagtacg gttgtgccct ctgatgacgt
     1681 gacggtggtt taccaaaatg gattaccggt gatatctgtg aggctaccct ctcgacgcga
     1741 gcgctgccaa ttcacactca agcctatctc tgactcagtc ggcgtgtttt tacgacaact
     1801 gcaagaggag gatcggggaa tcgaccgggt cgccatctac tcaccagatg gcgttcgagt
     1861 cgctgcctcc acggggatag acctcctgct cctcgatgac tttaagcttg tcattaatga
     1921 cttaacatac cacgtacggc caccaaagag agacctccta agccatgaag atgcagcgac
     1981 gctgaacgac gtgaagaccc tggtccagca gctgtacacc acactgtgca ttgagcagca
     2041 tcagcttaac aaagagcggg agctcgtgga gaggttagag gacctcaagc agcagctggc
     2101 ccccctggag aaggtacgaa ttgaaattag cagaaaagca gagaagagga ccactctggt
     2161 gctgtgggga ggcctggcct acatggccac ccagtttggc attctggccc ggctcacctg
     2221 gtgggagtac tcgtgggaca tcatggagcc cgtcacctac ttcatcacgt acggaagcgc
     2281 catggccatg tatgcgtatt ttgtaatgac gcgccaggaa tatgtttatc cagaagccag
     2341 agacagacaa tacttattat ttttccataa aggagccaaa aagtcacgtt tcgacctaga
     2401 gaaatacaat cagctcaagg atgcaattgc tcaggcagaa atggatctta agagactgag
     2461 agacccatta caagtacacc tgcccctccg acagatcgga gaaaaggaag ctagcaccat
     2521 ggtggcctcc tccgaggacg tcatcaaaga gttcatgcgc ttcaaggtgc gcatggaggg
     2581 ctccgtgaac ggccacgagt tcgagatcga gggcgagggc gagggccgcc cctacgaggg
     2641 cacccagacc gccaagctga aggtgaccaa gggcggcccc ctgcccttcg cctgggacat
     2701 cctgtccccc cagttccagt acggctccaa ggcgtacgtg aagcaccccg ccgacatccc
     2761 cgactacaag aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga
     2821 ggacggcggc gtggtgaccg tgacccagga ctcctccctg caggacggca cgctgatcta
     2881 caaggtgaag ttccgcggca ccaacttccc ccccgacggc cccgtaatgc agaagaagac
     2941 catgggctgg gaggcctcca ccgagcgcct gtacccccgc gacggcgtgc tgaagggcga
     3001 gatccaccag gccctgaagc tgaaggacgg cggccactac ctggtggagt tcaagaccat
     3061 ctacatggcc aagaagcccg tgcagctgcc cggctactac tacgtggaca ccaagctgga
     3121 catcacctcc cacaacgagg actacaccat cgtggaacag tacgagcgct ccgagggccg
     3181 ccaccacctg ttcctggggc atggcaccgg cagcaccggc agcggcagct ccggcaccgc
     3241 ctcctccgag gacgtcatca aagagttcat gcgcttcaag gtgcgcatgg agggctccgt
     3301 gaacggccac gagttcgaga tcgagggcga gggcgagggc cgcccctacg agggcaccca
     3361 gaccgccaag ctgaaggtga ccaagggcgg ccccctgccc ttcgcctggg acatcctgtc
     3421 cccccagttc cagtacggct ccaaggcgta cgtgaagcac cccgccgaca tccccgacta
     3481 caagaagctg tccttccccg agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg
     3541 cggcgtggtg accgtgaccc aggactcctc cctgcaggac ggcacgctga tctacaaggt
     3601 gaagttccgc ggcaccaact tcccccccga cggccccgta atgcagaaga agaccatggg
     3661 ctgggaggcc tccaccgagc gcctgtaccc ccgcgacggc gtgctgaagg gcgagatcca
     3721 ccaggccctg aagctgaagg acggcggcca ctacctggtg gagttcaaga ccatctacat
     3781 ggccaagaag cccgtgcagc tgcccggcta ctactacgtg gacaccaagc tggacatcac
     3841 ctcccacaac gaggactaca ccatcgtgga acagtacgag cgctccgagg gccgccacca
     3901 cctgttcctg taggaattcg atatcaagct tatcgataat caacctctgg attacaaaat
     3961 ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc
     4021 tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt
     4081 gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg
     4141 cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg
     4201 tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc
     4261 cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt
     4321 gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca cctggattct
     4381 gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg
     4441 cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg
     4501 gatctccctt tgggccgcct ccccgcatcg ataccgtcga cctcgagaga tctacgggtg
     4561 gcatccctgt gacccctccc cagtgcctct cctggccctg gaagttgcca ctccagtgcc
     4621 caccagcctt gtcctaataa aattaagttg catcattttg tctgactagg tgtccttcta
     4681 taatattatg gggtggaggg gggtggtatg gagcaagggg caagttggga agacaacctg
     4741 tagggcctgc ggggtctatt gggaaccaag ctggagtgca gtggcacaat cttggctcac
     4801 tgcaatctcc gcctcctggg ttcaagcgat tctcctgcct cagcctcccg agttgttggg
     4861 attccaggca tgcatgacca ggctcagcta atttttgttt ttttggtaga gacggggttt
     4921 caccatattg gccaggctgg tctccaactc ctaatctcag gtgatctacc caccttggcc
     4981 tcccaaattg ctgggattac aggcgtgaac cactgctccc ttccctgtcc ttctgatttt
     5041 gtaggtaacc acgtgcggac cgagcggccg caggaacccc tagtgatgga gttggccact
     5101 ccctctctgc gcgctcgctc gctcactgag gccgggcgac caaaggtcgc ccgacgcccg
     5161 ggctttgccc gggcggcctc agtgagcgag cgagcgcgca gctgcctgca ggggcgcctg
     5221 atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatacg tcaaagcaac
     5281 catagtacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
     5341 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
     5401 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
     5461 gatttagtgc tttacggcac ctcgacccca aaaaacttga tttgggtgat ggttcacgta
     5521 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
     5581 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcgggc tattcttttg
     5641 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa
     5701 aatttaacgc gaattttaac aaaatattaa cgtttacaat tttatggtgc actctcagta
     5761 caatctgctc tgatgccgca tagttaagcc agccccgaca cccgccaaca cccgctgacg
     5821 cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg
     5881 ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc
     5941 tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag
     6001 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
     6061 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
     6121 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
     6181 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
     6241 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
     6301 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
     6361 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
     6421 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
     6481 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
     6541 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
     6601 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
     6661 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
     6721 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
     6781 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
     6841 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
     6901 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
     6961 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     7021 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     7081 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     7141 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     7201 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     7261 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc
     7321 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     7381 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     7441 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     7501 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     7561 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     7621 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     7681 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     7741 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     7801 ctcacatgt
//
