LOCUS       Exported                6527 bp DNA     circular SYN 17-MAR-2022
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6527)
  AUTHORS   Anna M. H. Hertle
  TITLE     Direct Submission
  JOURNAL   Exported Mar 17, 2022 from SnapGene Viewer 6.0.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6527
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..190
                     /label=ITR
                     /note="Inverted terminal repeat1..190"
                     /note="color: #008b8b; direction: RIGHT"
     promoter uukaryotic191..541
                     /direction=RIGHT
                     /note="U6 Promoter for shRNA transcription191..541"
                     /note="color: #84a4c0"
     primer_bind     431..455
                     /label=U6_for
                     /note="color: #a020f0; direction: RIGHT"
     misc_feature    478..1003
                     /label=sequence okay
                     /note="201208"
                     /note="color: #a6acb3"
     misc_RNA        550..569
                     /label=sh1(MsMCU) MCU target sequence
                     /note="color: #993300; direction: RIGHT"
     promoter        join(638..886,888..1397,1481..1574)
                     /label=CAG promoter
                     /note="CAG promoter"
                     /note="This forward directional feature has 4 segments:
                        1:  638 ..  886 / #ffffff / CMV enhancer
                        2:  888 .. 1165 / #ffffff / chicken beta-actin promoter
                        3: 1166 .. 1397 / #ffffff / chicken beta-actin precur...
                        4: 1481 .. 1574 / #ffffff / rabbit beta globin 
                     splice..."
     primer_bind     1447..1465
                     /label=CMV_for
                     /note="color: #a020f0; direction: RIGHT"
     misc_feature    1500..2684
                     /label=sequence okay
                     /note="201208"
                     /note="color: #a6acb3"
     CDS             1588..2307
                     /label=hrGFP
                     /note="hrGFP reporter gene1586..2305"
                     /note="color: #ff4500; direction: RIGHT"
     misc_feature    2339..2927
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
                     /note="color: #ffffff"
     primer_bind     complement(2425..2443)
                     /label=WPRE_rev
                     /note="color: #a020f0; direction: LEFT"
     CDS             complement(2810..2821)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /note="color: #cc99b2
                     Cleavage site after base 2809"
                     /translation="IEGR"
     polyA_signal    2960..3184
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
                     /note="color: #a6acb3"
     LTR             complement(3218..3399)
                     /label=ITR
                     /note="Inverted Repeatcomplement(3216..3397)"
                     /note="color: #008b8b; direction: LEFT"
     promoter        complement(3405..3734)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
                     /note="color: #ffffff; direction: LEFT"
     rep_origin      3419..3554
                     /label=SV40 ori
                     /note="SV40 origin of replication"
                     /note="color: #ffff00"
     rep_origin      complement(3980..4568)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     CDS             complement(4739..5599)
                     /label=Amp R
                     /note="complement(4737..5597)"
                     /note="color: #ff4500; direction: LEFT"
     promoter        complement(5600..5704)
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: LEFT"
     promoter        6492..6510
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
                     /note="color: #ffffff; direction: RIGHT"
ORIGIN
        1 tagctgcgcg ctcgctcgct cactgaggcc gcccgggcaa agcccgggcg tcgggcgacc
       61 tttggtcgcc cggcctcagt gagcgagcga gcgcgcagag agggagtggc caactccatc
      121 actaggggtt ccttgtagtt aatgattaac ccgccatgct acttatctac gtagccatgc
      181 tctaggtacc ctcgaggagg agaagcatga attccccagt ggaaagacgc gcaggcaaaa
      241 cgcaccacgt gacggagcgt gaccgcgcgc cgagcgcgcg ccaaggtcgg gcaggaagag
      301 ggcctatttc ccatgattcc ttcatatttg catatacgat acaaggctgt tagagagata
      361 attagaatta atttgactgt aaacacaaag atattagtac aaaatacgtg acgtagaaag
      421 taataatttc ttgggtagtt tgcagtttta aaattatgtt ttaaaatgga ctatcatatg
      481 cttaccgtaa cttgaaagta tttcgatttc ttgggtttat atatcttgtg gaaaggacgc
      541 gggatcccct agggaataaa gggatcttaa ttcaagagat taagatccct ttattcccta
      601 tttttggaaa agcttggcac tggccgtact agtggtacca ttgacgtcaa taatgacgta
      661 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
      721 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
      781 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
      841 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtcg aggtgagccc
      901 cacgttctgc ttcactctcc ccatctcccc cccctcccca cccccaattt tgtatttatt
      961 tattttttaa ttattttgtg cagcgatggg ggcggggggg gggggggggc gcgcgccagg
     1021 cggggcgggg cggggcgagg ggcggggcgg ggcgaggcgg agaggtgcgg cggcagccaa
     1081 tcagagcggc gcgctccgaa agtttccttt tatggcgagg cggcggcggc ggcggcccta
     1141 taaaaagcga agcgcgcggc gggcgggagt cgctgcgacg ctgccttcgc cccgtgcccc
     1201 gctccgccgc cgcctcgcgc cgcccgcccc ggctctgact gaccgcgtta ctcccacagg
     1261 tgagcgggcg ggacggccct tctcctccgg gctgtaatta gcgcttggtt taatgacggc
     1321 ttgtttcttt tctgtggctg cgtgaaagcc ttgaggggct ccgggagggc cctttgtgcg
     1381 gggggagcgg ctcggggctg tccgcggggg gacggctgcc ttcggggggg acggggcagg
     1441 gcggggttcg gcttctggcg tgtgaccggc ggctctagag cctctgctaa ccatgttcat
     1501 gccttcttct ttttcctaca gctcctgggc aacgtgctgg ttattgtgct gtctcatcat
     1561 tttggcaaag aattggatcg atccaccatg gtgagcaagc agatcctgaa gaacaccggc
     1621 ctgcaggaga tcatgagctt caaggtgaac ctggagggcg tggtgaacaa ccacgtgttc
     1681 accatggagg gctgcggcaa gggcaacatc ctgttcggca accagctggt gcagatccgc
     1741 gtgaccaagg gcgcccccct gcccttcgcc ttcgacatcc tgagccccgc cttccagtac
     1801 ggcaaccgca ccttcaccaa gtaccccgag gacatcagcg acttcttcat ccagagcttc
     1861 cccgccggct tcgtgtacga gcgcaccctg cgctacgagg acggcggcct ggtggagatc
     1921 cgcagcgaca tcaacctgat cgaggagatg ttcgtgtacc gcgtggagta caagggccgc
     1981 aacttcccca acgacggccc cgtgatgaag aagaccatca ccggcctgca gcccagcttc
     2041 gaggtggtgt acatgaacga cggcgtgctg gtgggccagg tgatcctggt gtaccgcctg
     2101 aacagcggca agttctacag ctgccacatg cgcaccctga tgaagagcaa gggcgtggtg
     2161 aaggacttcc ccgagtacca cttcatccag caccgcctgg agaagaccta cgtggaggac
     2221 ggcggcttcg tggagcagca cgagaccgcc atcgcccagc tgaccagcct gggcaagccc
     2281 ctgggcagcc tgcacgagtg ggtgtaatag gaattcgata tcaagctagc ttatcgataa
     2341 tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc
     2401 ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat
     2461 ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg
     2521 gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg
     2581 ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat
     2641 tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt
     2701 gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc
     2761 ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa
     2821 tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg
     2881 ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcatc gataccgtcg
     2941 actcgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc
     3001 cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag
     3061 gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag
     3121 gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct
     3181 atggcttctg aggcggaaag aaccagctgg ggctcgacta gagcatggct acgtagataa
     3241 gtagcatggc gggttaatca ttaactacaa ggaaccccta gtgatggagt tggccactcc
     3301 ctctctgcgc gctcgctcgc tcactgaggc cgggcgacca aaggtcgccc gacgcccggg
     3361 ctttgcccgg gcggcctcag tgagcgagcg agcgcgcaga gctttttgca aaagcctagg
     3421 cctccaaaaa agcctcctca ctacttctgg aatagctcag aggccgaggc ggcctcggcc
     3481 tctgcataaa taaaaaaaat tagtcagcca tggggcggag aatgggcgga actgggcgga
     3541 gttaggggcg ggatgggcgg agttaggggc gggactatgg ttgctgacta attgagatgc
     3601 atgctttgca tacttctgcc tgctggggag cctggggact ttccacacct ggttgctgac
     3661 taattgagat gcatgctttg catacttctg cctgctgggg agcctgggga ctttccacac
     3721 cctaactgac acacattcca cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
     3781 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     3841 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     3901 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     3961 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     4021 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     4081 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     4141 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     4201 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     4261 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     4321 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     4381 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
     4441 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     4501 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     4561 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     4621 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     4681 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     4741 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     4801 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     4861 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
     4921 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     4981 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     5041 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     5101 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     5161 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     5221 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     5281 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     5341 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
     5401 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     5461 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
     5521 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
     5581 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
     5641 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
     5701 cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat
     5761 taacctataa aaataggcgt atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg
     5821 gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg
     5881 ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc
     5941 ttaactatgc ggcatcagag cagattgtac tgagagtgca ccattcgacg ctctccctta
     6001 tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc
     6061 gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc
     6121 accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca
     6181 tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc
     6241 acgatgcgtc cggcgtagag gatctggcta gcgatgaccc tgctgattgg ttcgctgacc
     6301 atttccgggt gcgggacggc gttaccagaa actcagaagg ttcgtccaac caaaccgact
     6361 ctgacggcag tttacgagag agatgatagg gtctgcttca gtaagccaga tgctacacaa
     6421 ttaggcttgt acatattgtc gttagaacgc ggctacaatt aatacataac cttatgtatc
     6481 atacacatac gatttaggtg acactataga atacacggaa ttaattc
//
