LOCUS       Exported                9817 bp DNA     circular SYN 15-FEB-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 9817)
  AUTHORS   Giovanni Forcina
  TITLE     Direct Submission
  JOURNAL   Exported Feb 15, 2022 from SnapGene 6.0.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9817
                     /mol_type="other DNA"
                     /organism="recombinant plasmid"
     promoter        446..475
                     /label=lac
     misc_binding    480..502
                     /label=LacO
     primer_bind     508..528
                     /label=M13-rev
     primer_bind     546..565
                     /label=T3
     LTR             818..998
                     /label=HIV-1 5 LTR
     misc_feature    1109..1153
                     /label=HIV-1 psi pack
     misc_feature    1663..1896
                     /label=RRE
     promoter        2531..3057
                     /label=CMV promoter
     primer_bind     3143..3200
                     /label=attB Fragment.FOR
     protein_bind    3143..3167
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_recomb     3144..3150
                     /label=AttR1
     misc_feature    complement(3151)
                     /label=attL1
                     /note="label=""attL1"" - complement(569..652)"
     misc_feature    3155..5021
                     
                     /note="note=""Fragment inserted from 
                     NFE2L2_1818_linear.xdna into pDONR223.xdna [LR] reaction"
     misc_feature    3182..4998
                     /label=NFE2L2_1818
     primer_bind     complement(4976..5036)
                     /label=attB Fragment.REV
     protein_bind    complement(5012..5036)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_recomb     complement(5022..5035)
                     /label=AttR2
     CDS             complement(5538..5549)
                     /label=FactorXa site
     promoter        5667..6006
                     /label=mPGK Prom
     LTR             6979..7159
                     /label=HIV-1 5 LTR
     misc_feature    7392..7469
                     /label=SV40 ORI
     primer_bind     complement(7547..7566)
                     /label=T7
     primer_bind     complement(7576..7593)
                     /label=M13-fwd
     CDS             7664..7732
                     /label=LacZ alpha
     rep_origin      complement(7739..8179)
                     /direction=LEFT
                     /label=F1 ori
     promoter        8250..8278
                     /label=Amp prom
     CDS             8518..9177
                     /label=AmpR
ORIGIN
        1 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
       61 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
      121 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
      181 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
      241 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
      301 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
      361 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt
      421 agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg
      481 gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc
      541 gcgcaattaa ccctcactaa agggaacaaa agctggagct gcaagcttaa tgtagtctta
      601 tgcaatactc ttgtagtctt gcaacatggt aacgatgagt tagcaacatg ccttacaagg
      661 agagaaaaag caccgtgcat gccgattggt ggaagtaagg tggtacgatc gtgccttatt
      721 aggaaggcaa cagacgggtc tgacatggat tggacgaacc actgaattgc cgcattgcag
      781 agatattgta tttaagtgcc tagctcgata cataaacggg tctctctggt tagaccagat
      841 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
      901 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
      961 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg
     1021 aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc
     1081 acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac tagcggaggc
     1141 tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat tagatcgcga
     1201 tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa acatatagta
     1261 tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga aacatcagaa
     1321 ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc agaagaactt
     1381 agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat agagataaaa
     1441 gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa gaccaccgca
     1501 cagcaagcgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1561 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1621 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1681 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1741 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1801 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1861 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1921 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1981 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     2041 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     2101 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     2161 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     2221 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     2281 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     2341 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcggttaa cttttaaaag
     2401 aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa tagcaacaga
     2461 catacaaact aaagaattac aaaaacaaat tacaaaattc aaaattttat cgataagctt
     2521 gggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
     2581 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
     2641 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
     2701 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
     2761 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
     2821 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
     2881 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
     2941 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
     3001 gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac cgtcagatcg
     3061 cctggagacg ccatccacgc tgttttgacc tccatagaag acaccgactc tagtccagtg
     3121 tggtggaatt ctgcagatat caacaagttt gtacaaaaaa gcaggcttaa aaaaagttgg
     3181 catgatggac ttggagctgc cgccgccggg actcccgtcc cagcaggaca tggatttgat
     3241 tgacatactt tggaggcaag atatagatct tggagtaagt cgagaagtat ttgacttcag
     3301 tcagcgacgg aaagagtatg agctggaaaa acagaaaaaa cttgaaaagg aaagacaaga
     3361 acaactccaa aaggagcaag agaaagcctt tttcgctcag ttacaactag atgaagagac
     3421 aggtgaattt ctcccaattc agccagccca gcacatccag tcagaaacca gtggatctgc
     3481 caactactcc caggttgccc acattcccaa atcagatgct ttgtactttg atgactgcat
     3541 gcagcttttg gcgcagacat tcccgtttgt agatgacaat gaggtttctt cggctacgtt
     3601 tcagtcactt gttcctgata ttcccggtca catcgagagc ccagtcttca ttgctactaa
     3661 tcaggctcag tcacctgaaa cttctgttgc tcaggtagcc cctgttgatt tagacggtat
     3721 gcaacaggac attgagcaag tttgggagga gctattatcc attcctgagt tacagtgtct
     3781 taatattgaa aatgacaagc tggttgagac taccatggtt ccaagtccag aagccaaact
     3841 gacagaagtt gacaattatc atttttactc atctataccc tcaatggaaa aagaagtagg
     3901 taactgtagt ccacattttc ttaatgcttt tgaggattcc ttcagcagca tcctctccac
     3961 agaagacccc aaccagttga cagtgaactc attaaattca gatgccacag tcaacacaga
     4021 ttttggtgat gaattttatt ctgctttcat agctgagccc agtatcagca acagcatgcc
     4081 ctcacctgct actttaagcc attcactctc tgaacttcta aatgggccca ttgatgtttc
     4141 tgatctatca ctttgcaaag ctttcaacca aaaccaccct gaaagcacag cagaattcaa
     4201 tgattctgac tccggcattt cactaaacac aagtcccagt gtggcatcac cagaacactc
     4261 agtggaatct tccagctatg gagacacact acttggcctc agtgattctg aagtggaaga
     4321 gctagatagt gcccctggaa gtgtcaaaca gaatggtcct aaaacaccag tacattcttc
     4381 tggggatatg gtacaaccct tgtcaccatc tcaggggcag agcactcacg tgcatgatgc
     4441 ccaatgtgag aacacaccag agaaagaatt gcctgtaagt cctggtcatc ggaaaacccc
     4501 attcacaaaa gacaaacatt caagccgctt ggaggctcat ctcacaagag atgaacttag
     4561 ggcaaaagct ctccatatcc cattccctgt agaaaaaatc attaacctcc ctgttgttga
     4621 cttcaacgaa atgatgtcca aagagcagtt caatgaagct caacttgcat taattcggga
     4681 tatacgtagg aggggtaaga ataaagtggc tgctcagaat tgcagaaaaa gaaaactgga
     4741 aaatatagta gaactagagc aagatttaga tcatttgaaa gatgaaaaag aaaaattgct
     4801 caaagaaaaa ggagaaaatg acaaaagcct tcacctactg aaaaaacaac tcagcacctt
     4861 atatctcgaa gttttcagca tgctacgtga tgaagatgga aaaccttatt ctcctagtga
     4921 atactccctg cagcaaacaa gagatggcaa tgttttcctt gttcccaaaa gtaagaagcc
     4981 agatgttaag aaaaactacc caactttctt aacccagctt tcttgtacaa agtggttgat
     5041 atccagcaca gtggcggccg ctcgacaatc aacctctgga ttacaaaatt tgtgaaagat
     5101 tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc
     5161 ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct
     5221 ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca
     5281 ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt
     5341 ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg
     5401 cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga
     5461 agctgacgtc ctttccatgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt
     5521 ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc
     5581 cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt
     5641 gggccgcctc cccgcctgga attctaccgg gtaggggagg cgcttttccc aaggcagtct
     5701 ggagcatgcg ctttagcagc cccgctgggc acttggcgct acacaagtgg cctctggcct
     5761 cgcacacatt ccacatccac cggtaggcgc caaccggctc cgttctttgg tggccccttc
     5821 gcgccacctt ctactcctcc cctagtcagg aagttccccc ccgccccgca gctcgcgtcg
     5881 tgcaggacgt gacaaatgga agtagcacgt ctcactagtc tcgtgcagat ggacagcacc
     5941 gctgagcaat ggaagcgggt aggcctttgg ggcagcggcc aatagcagct ttgctccttc
     6001 gctttctggg ctcagaggct gggaaggggt gggtccgggg gcgggctcag gggcgggctc
     6061 aggggcgggg cgggcgcccg aaggtcctcc ggaggcccgg cattctgcac gcttcaaaag
     6121 cgcacgtctg ccgcgctgtt ctcctcttcc tcatctccgg gcctttcgac ctgcagccca
     6181 agcttaccat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca
     6241 gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg
     6301 atccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg
     6361 ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca
     6421 cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt
     6481 tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc
     6541 ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg
     6601 gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg
     6661 ccttcctgga gacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg
     6721 tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg
     6781 gtgcctgacg cccgccccac gacccgcagc gcccgaccga aaggagcgca cgaccccatg
     6841 catctcgagg gcccggtacc tttaagacca atgacttaca aggcagctgt agatcttagc
     6901 cactttttaa aagaaaaggg gggactggaa gggctagctc actcccaacg aagacaagat
     6961 ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct
     7021 ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta
     7081 gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca
     7141 gtgtggaaaa tctctagcag tagtagttca tgtcatctta ttattcagta tttataactt
     7201 gcaaagaaat gaatatcaga gagtgagagg aacttgttta ttgcagctta taatggttac
     7261 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
     7321 tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggctctagct atcccgcccc
     7381 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
     7441 gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga
     7501 agtagtgagg aggctttttt ggaggcctag ggacgtaccc aattcgccct atagtgagtc
     7561 gtattacgcg cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt
     7621 tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga
     7681 ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat gggacgcgcc
     7741 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact
     7801 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc
     7861 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt
     7921 acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc
     7981 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt
     8041 gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat
     8101 tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa
     8161 ttttaacaaa atattaacgc ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga
     8221 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     8281 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
     8341 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
     8401 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
     8461 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
     8521 agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag
     8581 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
     8641 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
     8701 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
     8761 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
     8821 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg
     8881 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
     8941 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
     9001 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg
     9061 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
     9121 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
     9181 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
     9241 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
     9301 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     9361 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     9421 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     9481 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
     9541 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     9601 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     9661 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     9721 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     9781 gacaggtatc cggtaagcgg cagggtcgga acaggag
//
