LOCUS       pRB035                  3759 bp ds-DNA     circular     11-JAN-2022
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    2..684
                     /label="ColE1 origin"
     misc_feature    685..794
                     /label="T0"
     misc_feature    795..1470
                     /label="CmR"
     misc_feature    1677..1730
                     /label="PLTetO-1"
     promoter        1731..1750
                     /label="PLTetO-1"
     misc_feature    1751..1835
                     /label="apFAB700 (BCD22)"
     misc_feature    1836..2546
                     /label="mNeonGreen"
     misc_feature    2547..2623
                     /label="Terminator B0015"
     misc_feature    2828..2862
                     /label="J23106 Promoter"
     misc_feature    2863..2896
                     /label="sRBS(50,000)"
     misc_feature    2897..3523
                     /label="tetR"
     misc_feature    3524..3628
                     /label="T1 ter"
ORIGIN
        1 AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC
       61 GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC
      121 CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG
      181 CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCAATGCTC ACGCTGTAGG TATCTCAGTT
      241 CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC
      301 GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC
      361 CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG
      421 AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG GACAGTATTT GGTATCTGCG
      481 CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA
      541 CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG
      601 GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT
      661 CACGTTAAGG GATTTTGGTC ATGACTAGTG CTTGGATTCT CACCAATAAA AAACGCCCGG
      721 CGGCAACCGA GCGTTCTGAA CAAATCCAGA TGGAGTTCTG AGGTCATTAC TGGATCTATC
      781 AACAGGAGTC CAAGCGAGCT CGATATCAAA TTACGCCCCG CCCTGCCACT CATCGCAGTA
      841 CTGTTGTAAT TCATTAAGCA TTCTGCCGAC ATGGAAGCCA TCACAGACGG CATGATGAAC
      901 CTGAATCGCC AGCGGCATCA GCACCTTGTC GCCTTGCGTA TAATATTTGC CCATGGTGAA
      961 AACGGGGGCG AAGAAGTTGT CCATATTGGC CACGTTTAAA TCAAAACTGG TGAAACTCAC
     1021 CCAGGGATTG GCTGAGACGA AAAACATATT CTCAATAAAC CCTTTAGGGA AATAGGCCAG
     1081 GTTTTCACCG TAACACGCCA CATCTTGCGA ATATATGTGT AGAAACTGCC GGAAATCGTC
     1141 GTGGTATTCA CTCCAGAGCG ATGAAAACGT TTCAGTTTGC TCATGGAAAA CGGTGTAACA
     1201 AGGGTGAACA CTATCCCATA TCACCAGCTC ACCGTCTTTC ATTGCCATAC GAAATTCCGG
     1261 ATGAGCATTC ATCAGGCGGG CAAGAATGTG AATAAAGGCC GGATAAAACT TGTGCTTATT
     1321 TTTCTTTACG GTCTTTAAAA AGGCCGTAAT ATCCAGCTGA ACGGTCTGGT TATAGGTACA
     1381 TTGAGCAACT GACTGAAATG CCTCAAAATG TTCTTTACGA TGCCATTGGG ATATATCAAC
     1441 GGTGGTATAT CCAGTGATTT TTTTCTCCAT TTTAGCTTCC TTAGCTCCTG AAAATCTCGA
     1501 TAACTCAAAA AATACGCCCG GTAGTGATCT TATTTCATTA TGGTGAAAGT TGGAACCTCT
     1561 TACGTGCCGA TCAACGTCTC ATTTTCGCCA GATATCGACG TCTAAGAAAC CATTATTATC
     1621 ATGACATTAA CCTATAAAAA TAGGCGTATC ACGAGGCCCT TTCGTCTTCA CCTCGATCCC
     1681 TATCAGTGAT AGAGATTGAC ATCCCTATCA GTGATAGAGA TACTGAGCAC ATCAGCAGGA
     1741 CGCACTGACC GGGCCCAAGT TCACTTAAAA AGGAGATCAA CAATGAAAGC AATTTTCGTA
     1801 CTGAAACATC TTAATCATGC CTAGGAAGTT TTCTAATGGT TTCGAAAGGT GAGGAGGATA
     1861 ACATGGCGTC GCTGCCTGCT ACACATGAAC TGCATATTTT CGGCAGCATC AACGGAGTTG
     1921 ATTTCGACAT GGTGGGCCAA GGCACCGGCA ACCCGAACGA CGGCTATGAA GAATTGAATT
     1981 TGAAATCTAC CAAGGGCGAC TTGCAATTTA GTCCGTGGAT TCTGGTACCG CATATTGGTT
     2041 ATGGTTTTCA TCAGTATCTG CCCTACCCAG ACGGAATGAG CCCATTTCAG GCGGCTATGG
     2101 TGGATGGCAG TGGCTATCAG GTCCATCGCA CCATGCAATT CGAGGATGGG GCGAGCCTGA
     2161 CCGTGAATTA TCGGTACACT TACGAAGGTT CACATATTAA AGGTGAAGCA CAAGTTAAAG
     2221 GCACCGGCTT CCCGGCGGAT GGCCCAGTGA TGACCAACAG CCTGACGGCG GCGGACTGGT
     2281 GTCGTAGCAA AAAGACGTAT CCCAATGATA AAACAATTAT TTCTACCTTT AAATGGAGTT
     2341 ACACCACGGG TAACGGTAAA CGCTACCGTA GCACGGCTCG CACCACATAC ACCTTTGCGA
     2401 AACCCATGGC CGCGAACTAT CTGAAAAATC AGCCGATGTA TGTGTTTCGC AAAACGGAAC
     2461 TGAAACATTC GAAAACTGAA CTCAATTTCA AAGAGTGGCA AAAAGCCTTC ACCGACGTAA
     2521 TGGGCATGGA TGAACTGTAC AAATGACCAG GCATCAAATA AAACGAAAGG CTCAGTCGAA
     2581 AGACTGGGCC TTTCGTTTTA TCTGTTGTTT GTCGGTGAAC GCTCTCTACT AGAGTCACAC
     2641 TGGCTCACCT TCGGGTGGGC CTTTCTGCGT TTATAGTAAA TCACTGCATA ATTCGTGTCG
     2701 CTCAAGGCGC ACTCCCGTTC TGGATAATGT TTTTTGCGCC GACATCATAA CGGTTCTGGC
     2761 AAATATTCTG AAATGAGCTG CTCGTACCCG TGGATCCTCT AGAGTCGACC TGCAGGCATG
     2821 CAAGCTTTTT ACGGCTAGCT CAGTCCTAGG TATAGTGCTA GCCCAGCCAG AGAAACACTC
     2881 TTTAACAGGG GGTAGTATGA TGTCTCGTTT AGATAAAAGT AAAGTGATTA ACAGCGCATT
     2941 AGAGCTGCTT AATGAGGTCG GAATCGAAGG TTTAACAACC CGTAAACTCG CCCAGAAGCT
     3001 AGGTGTAGAG CAGCCTACAT TGTATTGGCA TGTAAAAAAT AAGCGGGCTT TGCTCGACGC
     3061 CTTAGCCATT GAGATGTTAG ATAGGCACCA TACTCACTTT TGCCCTTTAG AAGGGGAAAG
     3121 CTGGCAAGAT TTTTTACGTA ATAACGCTAA AAGTTTTAGA TGTGCTTTAC TAAGTCATCG
     3181 CGATGGAGCA AAAGTACATT TAGGTACACG GCCTACAGAA AAACAGTATG AAACTCTCGA
     3241 AAATCAATTA GCCTTTTTAT GCCAACAAGG TTTTTCACTA GAGAATGCAT TATATGCACT
     3301 CAGCGCAGTG GGGCATTTTA CTTTAGGTTG CGTATTGGAA GATCAAGAGC ATCAAGTCGC
     3361 TAAAGAAGAA AGGGAAACAC CTACTACTGA TAGTATGCCG CCATTATTAC GACAAGCTAT
     3421 CGAATTATTT GATCACCAAG GTGCAGAGCC AGCCTTCTTA TTCGGCCTTG AATTGATCAT
     3481 ATGCGGATTA GAAAAACAAC TTAAATGTGA AAGTGGGTCT TAAGGCATCA AATAAAACGA
     3541 AAGGCTCAGT CGAAAGACTG GGCCTTTCGT TTTATCTGTT GTTTGTCGGT GAACGCTCTC
     3601 CTGAGTAGGA CAAATCCGCC GCCCTAGACC TAGGCGTTCG GCTGCGGCGA GCGGTATCAG
     3661 CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA
     3721 TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAA
//