LOCUS       pRB117.2                5485 bp ds-DNA     circular     11-JAN-2022
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature                  33..86
                                   /label="PLTetO-1"
     promoter                      87..106
                                   /label="Lambda Phage Fragment"
     misc_feature                  107..191
                                   /label="apFAB700 (BCD22)"
     misc_feature                  192..899
                                   /label="mNeonGreen"
     misc_feature                  900..929
                                   /label="GGGGSX2 linker"
     misc_feature                  930..1580
                                   /label="NarL"
     misc_feature                  1581..1709
                                   /label="Terminator B0015"
     misc_feature                  1757..1807
                                   /label="narL-protected from DNaseI"
     misc_feature                  1757..1833
                                   /label="PNarL3"
     misc_feature                  1757..1833
                                   /label="PNarL3 (DcuS)"
     misc_feature                  1789..1795
                                   /label="narL heptamer"
     dna-binding                   1789..1804
                                   /label="narL 7-2-7 binding domain"
     misc_feature                  1797..1802
                                   /label="-35 sigma"
     misc_feature                  1798..1804
                                   /label="narL heptamer"
     misc_feature                  1819..1824
                                   /label="-10 sigma"
     transcription initiation site 1832..1832
                                   /label="sigma 70"
     misc_feature                  1834..1918
                                   /label="apFAB700 (BCD22)"
     misc_feature                  1919..2629
                                   /label="mCherry(RBS 5766)"
     terminator                    2630..2734
                                   /label="T1"
     terminator                    2735..2804
                                   /label="E. coli His operon terminator (a70c71 missing)"
     misc_feature                  2910..2944
                                   /label="J23106 Promoter"
     misc_feature                  2945..2978
                                   /label="sRBS(50,000)"
     misc_feature                  2979..3605
                                   /label="tetR"
     misc_feature                  3606..3710
                                   /label="T1 ter"
     misc_feature                  3843..4525
                                   /label="ColE1 origin"
     misc_feature                  4526..4635
                                   /label="T0"
     misc_feature                  4636..5311
                                   /label="CmR"
ORIGIN
        1 CGTATCACGA GGCCCTTTCG TCTTCACCTC GATCCCTATC AGTGATAGAG ATTGACATCC
       61 CTATCAGTGA TAGAGATACT GAGCACATCA GCAGGACGCA CTGACCGGGC CCAAGTTCAC
      121 TTAAAAAGGA GATCAACAAT GAAAGCAATT TTCGTACTGA AACATCTTAA TCATGCCTAG
      181 GAAGTTTTCT AATGGTTTCG AAAGGTGAGG AGGATAACAT GGCGTCGCTG CCTGCTACAC
      241 ATGAACTGCA TATTTTCGGC AGCATCAACG GAGTTGATTT CGACATGGTG GGCCAAGGCA
      301 CCGGCAACCC GAACGACGGC TATGAAGAAT TGAATTTGAA ATCTACCAAG GGCGACTTGC
      361 AATTTAGTCC GTGGATTCTG GTACCGCATA TTGGTTATGG TTTTCATCAG TATCTGCCCT
      421 ACCCAGACGG AATGAGCCCA TTTCAGGCGG CTATGGTGGA TGGCAGTGGC TATCAGGTCC
      481 ATCGCACCAT GCAATTCGAG GATGGGGCGA GCCTGACCGT GAATTATCGG TACACTTACG
      541 AAGGTTCACA TATTAAAGGT GAAGCACAAG TTAAAGGCAC CGGCTTCCCG GCGGATGGCC
      601 CAGTGATGAC CAACAGCCTG ACGGCGGCGG ACTGGTGTCG TAGCAAAAAG ACGTATCCCA
      661 ATGATAAAAC AATTATTTCT ACCTTTAAAT GGAGTTACAC CACGGGTAAC GGTAAACGCT
      721 ACCGTAGCAC GGCTCGCACC ACATACACCT TTGCGAAACC CATGGCCGCG AACTATCTGA
      781 AAAATCAGCC GATGTATGTG TTTCGCAAAA CGGAACTGAA ACATTCGAAA ACTGAACTCA
      841 ATTTCAAAGA GTGGCAAAAA GCCTTCACCG ACGTAATGGG CATGGATGAA CTGTACAAAG
      901 GTGGCGGTGG CTCTGGAGGT GGTGGGTCCa tgagtaatca ggaaccggct actatcctgc
      961 tgattgacga tcacccgatg ctgcgaactg gcgtaaaaca gcttatcagt atggcaccag
     1021 atatcaccgt ggttggcgaa gcgagtaatg gcgaacaggg tattgaactg gcggagtctc
     1081 ttgatcccga tctgatcctg ttagatctca atatgcccgg catgaacggt ctggaaacgc
     1141 tggataaact gcgcgaaaag tccctctcag ggcgcattgt ggtattcagc gtctctaacc
     1201 atgaagaaga tgtggtcacc gcactgaaac gcggcgcgga tggctatctg ttaaaagata
     1261 tggaaccgga agatctgctg aaagcattgc atcaggcagc tgctggcgaa atggtattaa
     1321 gcgaagcatt aacgcctgtt ctggccgcca gcttgcgcgc taaccgtgcc actactgagc
     1381 gcgatgttaa ccagttaacc ccacgcgagc gcgatattct caagctgatt gcccagggtt
     1441 tgccgaacaa gatgattgcc cgccgcctgg atatcaccga aagcacagta aaagtgcacg
     1501 tcaagcacat gctgaagaaa atgaagctca agtctcgcgt ggaagcagcg gtatgggtgc
     1561 atcaggagcg cattttcTGA CCAGGCATCA AATAAAACGA AAGGCTCAGT CGAAAGACTG
     1621 GGCCTTTCGT TTTATCTGTT GTTTGTCGGT GAACGCTCTC TACTAGAGTC ACACTGGCTC
     1681 ACCTTCGGGT GGGCCTTTCT GCGTTTATAG TAAATCACTG CATAATTCGT GTCGCTCAAG
     1741 GCGCACTCCC GTTCTGAGAG TCTCTTCCCC TATATAGCCA ATCATTCTTA CTCCCTTTGA
     1801 ATTACCCGCC TCATCAGAGA TAATGCTTAA GAAGGGCCCA AGTTCACTTA AAAAGGAGAT
     1861 CAACAATGAA AGCAATTTTC GTACTGAAAC ATCTTAATCA TGCCTAGGAA GTTTTCTAAT
     1921 GGTGAGCAAG GGCGAGGAGG ATAACATGGC CATCATCAAG GAGTTCATGC GCTTCAAGGT
     1981 GCACATGGAG GGCTCCGTGA ACGGCCACGA GTTCGAGATC GAGGGCGAGG GCGAGGGCCG
     2041 CCCCTACGAG GGCACCCAGA CCGCCAAGCT GAAGGTGACC AAGGGTGGCC CCCTGCCCTT
     2101 CGCCTGGGAC ATCCTGTCCC CTCAGTTCAT GTACGGCTCC AAGGCCTACG TGAAGCACCC
     2161 CGCCGACATC CCCGACTACT TGAAGCTGTC CTTCCCCGAG GGCTTCAAGT GGGAGCGCGT
     2221 GATGAACTTC GAGGACGGCG GCGTGGTGAC CGTGACCCAG GACTCCTCCC TGCAGGACGG
     2281 CGAGTTCATC TACAAGGTGA AGCTGCGCGG CACCAACTTC CCCTCCGACG GCCCCGTAAT
     2341 GCAGAAGAAG ACCATGGGCT GGGAGGCCTC CTCCGAGCGG ATGTACCCCG AGGACGGCGC
     2401 CCTGAAGGGC GAGATCAAGC AGAGGCTGAA GCTGAAGGAC GGCGGCCACT ACGACGCTGA
     2461 GGTCAAGACC ACCTACAAGG CCAAGAAGCC CGTGCAGCTG CCCGGCGCCT ACAACGTCAA
     2521 CATCAAGTTG GACATCACCT CCCACAACGA GGACTACACC ATCGTGGAAC AGTACGAACG
     2581 CGCCGAGGGC CGCCACTCCA CCGGCGGCAT GGACGAGCTG TACAAGTAAG GCATCAAATA
     2641 AAACGAAAGG CTCAGTCGAA AGACTGGGCC TTTCGTTTTA TCTGTTGTTT GTCGGTGAAC
     2701 GCTCTCCTGA GTAGGACAAA TCCGCCGCCC TAGATCCGGC AAAAAAGGGC AAGGTGTCAC
     2761 CACCCTGCCC TTTTTCTTTA AAACCGAAAA GATTACTTCG CGTTGATAAT GTTTTTTGCG
     2821 CCGACATCAT AACGGTTCTG GCAAATATTC TGAAATGAGC TGCTCGTACC CGTGGATCCT
     2881 CTAGAGTCGA CCTGCAGGCA TGCAAGCTTT TTACGGCTAG CTCAGTCCTA GGTATAGTGC
     2941 TAGCCCAGCC AGAGAAACAC TCTTTAACAG GGGGTAGTAT GATGTCTCGT TTAGATAAAA
     3001 GTAAAGTGAT TAACAGCGCA TTAGAGCTGC TTAATGAGGT CGGAATCGAA GGTTTAACAA
     3061 CCCGTAAACT CGCCCAGAAG CTAGGTGTAG AGCAGCCTAC ATTGTATTGG CATGTAAAAA
     3121 ATAAGCGGGC TTTGCTCGAC GCCTTAGCCA TTGAGATGTT AGATAGGCAC CATACTCACT
     3181 TTTGCCCTTT AGAAGGGGAA AGCTGGCAAG ATTTTTTACG TAATAACGCT AAAAGTTTTA
     3241 GATGTGCTTT ACTAAGTCAT CGCGATGGAG CAAAAGTACA TTTAGGTACA CGGCCTACAG
     3301 AAAAACAGTA TGAAACTCTC GAAAATCAAT TAGCCTTTTT ATGCCAACAA GGTTTTTCAC
     3361 TAGAGAATGC ATTATATGCA CTCAGCGCAG TGGGGCATTT TACTTTAGGT TGCGTATTGG
     3421 AAGATCAAGA GCATCAAGTC GCTAAAGAAG AAAGGGAAAC ACCTACTACT GATAGTATGC
     3481 CGCCATTATT ACGACAAGCT ATCGAATTAT TTGATCACCA AGGTGCAGAG CCAGCCTTCT
     3541 TATTCGGCCT TGAATTGATC ATATGCGGAT TAGAAAAACA ACTTAAATGT GAAAGTGGGT
     3601 CTTAAGGCAT CAAATAAAAC GAAAGGCTCA GTCGAAAGAC TGGGCCTTTC GTTTTATCTG
     3661 TTGTTTGTCG GTGAACGCTC TCCTGAGTAG GACAAATCCG CCGCCCTAGA CCTAGGCGTT
     3721 CGGCTGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA
     3781 GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA
     3841 AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT
     3901 CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC
     3961 CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC
     4021 GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCAATGCT CACGCTGTAG GTATCTCAGT
     4081 TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC
     4141 CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG
     4201 CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA
     4261 GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GGACAGTATT TGGTATCTGC
     4321 GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA
     4381 ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA
     4441 GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC
     4501 TCACGTTAAG GGATTTTGGT CATGACTAGT GCTTGGATTC TCACCAATAA AAAACGCCCG
     4561 GCGGCAACCG AGCGTTCTGA ACAAATCCAG ATGGAGTTCT GAGGTCATTA CTGGATCTAT
     4621 CAACAGGAGT CCAAGCGAGC TCGATATCAA ATTACGCCCC GCCCTGCCAC TCATCGCAGT
     4681 ACTGTTGTAA TTCATTAAGC ATTCTGCCGA CATGGAAGCC ATCACAGACG GCATGATGAA
     4741 CCTGAATCGC CAGCGGCATC AGCACCTTGT CGCCTTGCGT ATAATATTTG CCCATGGTGA
     4801 AAACGGGGGC GAAGAAGTTG TCCATATTGG CCACGTTTAA ATCAAAACTG GTGAAACTCA
     4861 CCCAGGGATT GGCTGAGACG AAAAACATAT TCTCAATAAA CCCTTTAGGG AAATAGGCCA
     4921 GGTTTTCACC GTAACACGCC ACATCTTGCG AATATATGTG TAGAAACTGC CGGAAATCGT
     4981 CGTGGTATTC ACTCCAGAGC GATGAAAACG TTTCAGTTTG CTCATGGAAA ACGGTGTAAC
     5041 AAGGGTGAAC ACTATCCCAT ATCACCAGCT CACCGTCTTT CATTGCCATA CGAAATTCCG
     5101 GATGAGCATT CATCAGGCGG GCAAGAATGT GAATAAAGGC CGGATAAAAC TTGTGCTTAT
     5161 TTTTCTTTAC GGTCTTTAAA AAGGCCGTAA TATCCAGCTG AACGGTCTGG TTATAGGTAC
     5221 ATTGAGCAAC TGACTGAAAT GCCTCAAAAT GTTCTTTACG ATGCCATTGG GATATATCAA
     5281 CGGTGGTATA TCCAGTGATT TTTTTCTCCA TTTTAGCTTC CTTAGCTCCT GAAAATCTCG
     5341 ATAACTCAAA AAATACGCCC GGTAGTGATC TTATTTCATT ATGGTGAAAG TTGGAACCTC
     5401 TTACGTGCCG ATCAACGTCT CATTTTCGCC AGATATCGAC GTCTAAGAAA CCATTATTAT
     5461 CATGACATTA ACCTATAAAA ATAGG
//