LOCUS       pRB133.2                5552 bp ds-DNA     circular     11-JAN-2022
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    810..863
                     /label="PLTetO-1"
     promoter        864..883
                     /label="Lambda Phage Fragment"
     misc_feature    884..968
                     /label="apFAB700 (BCD22)"
     gene            969..1640
                     /label="phoP gene"
     misc_feature    1641..1670
                     /label="GGGGSX2 linker"
     misc_feature    1671..2381
                     /label="mNeonGreen"
     misc_feature    2382..2510
                     /label="Terminator B0015"
     misc_feature    2558..2677
                     /label="PvirK"
     misc_feature    2564..2580
                     /label="PhoP Box"
     misc_feature    2607..2623
                     /label="PhoP Box"
     misc_feature    2614..2619
                     /label="-35"
     misc_feature    2636..2641
                     /label="-10"
     misc_feature    2678..2762
                     /label="apFAB700 (BCD22)"
     misc_feature    2763..3473
                     /label="mCherry(RBS 5766)"
     terminator      3474..3578
                     /label="T1"
     terminator      3579..3648
                     /label="E. coli His operon terminator (a70c71 missing)"
     misc_feature    3754..3788
                     /label="J23106 Promoter"
     misc_feature    3789..3822
                     /label="sRBS(50,000)"
     misc_feature    3823..4449
                     /label="tetR"
     misc_feature    4450..4554
                     /label="T1 ter"
     misc_feature    4687..5369
                     /label="ColE1 origin"
     misc_feature    5370..5479
                     /label="T0"
     misc_feature    join(5480..5552,1..603)
                     /label="CmR"
ORIGIN
        1 GACATGGAAG CCATCACAGA CGGCATGATG AACCTGAATC GCCAGCGGCA TCAGCACCTT
       61 GTCGCCTTGC GTATAATATT TGCCCATGGT GAAAACGGGG GCGAAGAAGT TGTCCATATT
      121 GGCCACGTTT AAATCAAAAC TGGTGAAACT CACCCAGGGA TTGGCTGAGA CGAAAAACAT
      181 ATTCTCAATA AACCCTTTAG GGAAATAGGC CAGGTTTTCA CCGTAACACG CCACATCTTG
      241 CGAATATATG TGTAGAAACT GCCGGAAATC GTCGTGGTAT TCACTCCAGA GCGATGAAAA
      301 CGTTTCAGTT TGCTCATGGA AAACGGTGTA ACAAGGGTGA ACACTATCCC ATATCACCAG
      361 CTCACCGTCT TTCATTGCCA TACGAAATTC CGGATGAGCA TTCATCAGGC GGGCAAGAAT
      421 GTGAATAAAG GCCGGATAAA ACTTGTGCTT ATTTTTCTTT ACGGTCTTTA AAAAGGCCGT
      481 AATATCCAGC TGAACGGTCT GGTTATAGGT ACATTGAGCA ACTGACTGAA ATGCCTCAAA
      541 ATGTTCTTTA CGATGCCATT GGGATATATC AACGGTGGTA TATCCAGTGA TTTTTTTCTC
      601 CATTTTAGCT TCCTTAGCTC CTGAAAATCT CGATAACTCA AAAAATACGC CCGGTAGTGA
      661 TCTTATTTCA TTATGGTGAA AGTTGGAACC TCTTACGTGC CGATCAACGT CTCATTTTCG
      721 CCAGATATCG ACGTCTAAGA AACCATTATT ATCATGACAT TAACCTATAA AAATAGGCGT
      781 ATCACGAGGC CCTTTCGTCT TCACCTCGAT CCCTATCAGT GATAGAGATT GACATCCCTA
      841 TCAGTGATAG AGATACTGAG CACATCAGCA GGACGCACTG ACCGGGCCCA AGTTCACTTA
      901 AAAAGGAGAT CAACAATGAA AGCAATTTTC GTACTGAAAC ATCTTAATCA TGCCTAGGAA
      961 GTTTTCTAat gatgcgcgta ctggttgtag aggataatgc attattacgc caccacctga
     1021 aggttcagct ccaggattca ggtcaccagg tcgatgccgc agaagatgcc agggaagctg
     1081 attactacct taatgaacac cttccggata tcgctattgt cgatttaggt ctgccggatg
     1141 aagacggcct ttccttaata cgccgctggc gcagcagtga tgtttcactg ccggttctgg
     1201 tgttaaccgc gcgcgaaggc tggcaggata aagtcgaggt tctcagctcc ggggccgatg
     1261 actacgtgac gaagccattc cacatcgaag aggtaatggc gcgtatgcag gcgttaatgc
     1321 gccgtaatag cggtctggcc tcccaggtga tcaacatccc gccgttccag gtggatctct
     1381 cacgccggga attatccgtc aatgaagagg tcatcaaact cacggcgttc gaatacacca
     1441 ttatggaaac gcttatccgt aacaacggta aagtggtcag caaagattcg ctgatgcttc
     1501 agctgtatcc ggatgcggaa ctgcgggaaa gtcataccat tgatgttctc atggggcgtc
     1561 tgcggaaaaa aatacaggcc cagtatccgc acgatgtcat taccaccgta cgcggacaag
     1621 gatatctttt tgaattgcgc GGTGGCGGTG GCTCTGGAGG TGGTGGGTCC ATGGTTTCGA
     1681 AAGGTGAGGA GGATAACATG GCGTCGCTGC CTGCTACACA TGAACTGCAT ATTTTCGGCA
     1741 GCATCAACGG AGTTGATTTC GACATGGTGG GCCAAGGCAC CGGCAACCCG AACGACGGCT
     1801 ATGAAGAATT GAATTTGAAA TCTACCAAGG GCGACTTGCA ATTTAGTCCG TGGATTCTGG
     1861 TACCGCATAT TGGTTATGGT TTTCATCAGT ATCTGCCCTA CCCAGACGGA ATGAGCCCAT
     1921 TTCAGGCGGC TATGGTGGAT GGCAGTGGCT ATCAGGTCCA TCGCACCATG CAATTCGAGG
     1981 ATGGGGCGAG CCTGACCGTG AATTATCGGT ACACTTACGA AGGTTCACAT ATTAAAGGTG
     2041 AAGCACAAGT TAAAGGCACC GGCTTCCCGG CGGATGGCCC AGTGATGACC AACAGCCTGA
     2101 CGGCGGCGGA CTGGTGTCGT AGCAAAAAGA CGTATCCCAA TGATAAAACA ATTATTTCTA
     2161 CCTTTAAATG GAGTTACACC ACGGGTAACG GTAAACGCTA CCGTAGCACG GCTCGCACCA
     2221 CATACACCTT TGCGAAACCC ATGGCCGCGA ACTATCTGAA AAATCAGCCG ATGTATGTGT
     2281 TTCGCAAAAC GGAACTGAAA CATTCGAAAA CTGAACTCAA TTTCAAAGAG TGGCAAAAAG
     2341 CCTTCACCGA CGTAATGGGC ATGGATGAAC TGTACAAATG ACCAGGCATC AAATAAAACG
     2401 AAAGGCTCAG TCGAAAGACT GGGCCTTTCG TTTTATCTGT TGTTTGTCGG TGAACGCTCT
     2461 CTACTAGAGT CACACTGGCT CACCTTCGGG TGGGCCTTTC TGCGTTTATA GTAAATCACT
     2521 GCATAATTCG TGTCGCTCAA GGCGCACTCC CGTTCTGtac cgccattgat aaactgttta
     2581 acaacatcgt ctgtacagac cttcttcgtt gcctttacgt ttaactcaat caggctaccg
     2641 tctcggttat aagtctatcg agtagtagag ccgtagtGGG CCCAAGTTCA CTTAAAAAGG
     2701 AGATCAACAA TGAAAGCAAT TTTCGTACTG AAACATCTTA ATCATGCCTA GGAAGTTTTC
     2761 TAATGGTGAG CAAGGGCGAG GAGGATAACA TGGCCATCAT CAAGGAGTTC ATGCGCTTCA
     2821 AGGTGCACAT GGAGGGCTCC GTGAACGGCC ACGAGTTCGA GATCGAGGGC GAGGGCGAGG
     2881 GCCGCCCCTA CGAGGGCACC CAGACCGCCA AGCTGAAGGT GACCAAGGGT GGCCCCCTGC
     2941 CCTTCGCCTG GGACATCCTG TCCCCTCAGT TCATGTACGG CTCCAAGGCC TACGTGAAGC
     3001 ACCCCGCCGA CATCCCCGAC TACTTGAAGC TGTCCTTCCC CGAGGGCTTC AAGTGGGAGC
     3061 GCGTGATGAA CTTCGAGGAC GGCGGCGTGG TGACCGTGAC CCAGGACTCC TCCCTGCAGG
     3121 ACGGCGAGTT CATCTACAAG GTGAAGCTGC GCGGCACCAA CTTCCCCTCC GACGGCCCCG
     3181 TAATGCAGAA GAAGACCATG GGCTGGGAGG CCTCCTCCGA GCGGATGTAC CCCGAGGACG
     3241 GCGCCCTGAA GGGCGAGATC AAGCAGAGGC TGAAGCTGAA GGACGGCGGC CACTACGACG
     3301 CTGAGGTCAA GACCACCTAC AAGGCCAAGA AGCCCGTGCA GCTGCCCGGC GCCTACAACG
     3361 TCAACATCAA GTTGGACATC ACCTCCCACA ACGAGGACTA CACCATCGTG GAACAGTACG
     3421 AACGCGCCGA GGGCCGCCAC TCCACCGGCG GCATGGACGA GCTGTACAAG TAAGGCATCA
     3481 AATAAAACGA AAGGCTCAGT CGAAAGACTG GGCCTTTCGT TTTATCTGTT GTTTGTCGGT
     3541 GAACGCTCTC CTGAGTAGGA CAAATCCGCC GCCCTAGATC CGGCAAAAAA GGGCAAGGTG
     3601 TCACCACCCT GCCCTTTTTC TTTAAAACCG AAAAGATTAC TTCGCGTTGA TAATGTTTTT
     3661 TGCGCCGACA TCATAACGGT TCTGGCAAAT ATTCTGAAAT GAGCTGCTCG TACCCGTGGA
     3721 TCCTCTAGAG TCGACCTGCA GGCATGCAAG CTTTTTACGG CTAGCTCAGT CCTAGGTATA
     3781 GTGCTAGCCC AGCCAGAGAA ACACTCTTTA ACAGGGGGTA GTATGATGTC TCGTTTAGAT
     3841 AAAAGTAAAG TGATTAACAG CGCATTAGAG CTGCTTAATG AGGTCGGAAT CGAAGGTTTA
     3901 ACAACCCGTA AACTCGCCCA GAAGCTAGGT GTAGAGCAGC CTACATTGTA TTGGCATGTA
     3961 AAAAATAAGC GGGCTTTGCT CGACGCCTTA GCCATTGAGA TGTTAGATAG GCACCATACT
     4021 CACTTTTGCC CTTTAGAAGG GGAAAGCTGG CAAGATTTTT TACGTAATAA CGCTAAAAGT
     4081 TTTAGATGTG CTTTACTAAG TCATCGCGAT GGAGCAAAAG TACATTTAGG TACACGGCCT
     4141 ACAGAAAAAC AGTATGAAAC TCTCGAAAAT CAATTAGCCT TTTTATGCCA ACAAGGTTTT
     4201 TCACTAGAGA ATGCATTATA TGCACTCAGC GCAGTGGGGC ATTTTACTTT AGGTTGCGTA
     4261 TTGGAAGATC AAGAGCATCA AGTCGCTAAA GAAGAAAGGG AAACACCTAC TACTGATAGT
     4321 ATGCCGCCAT TATTACGACA AGCTATCGAA TTATTTGATC ACCAAGGTGC AGAGCCAGCC
     4381 TTCTTATTCG GCCTTGAATT GATCATATGC GGATTAGAAA AACAACTTAA ATGTGAAAGT
     4441 GGGTCTTAAG GCATCAAATA AAACGAAAGG CTCAGTCGAA AGACTGGGCC TTTCGTTTTA
     4501 TCTGTTGTTT GTCGGTGAAC GCTCTCCTGA GTAGGACAAA TCCGCCGCCC TAGACCTAGG
     4561 CGTTCGGCTG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA
     4621 ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG
     4681 TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA
     4741 AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT
     4801 TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT
     4861 GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAA TGCTCACGCT GTAGGTATCT
     4921 CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC
     4981 CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT
     5041 ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC
     5101 TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGGACAG TATTTGGTAT
     5161 CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA
     5221 ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA
     5281 AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA
     5341 AAACTCACGT TAAGGGATTT TGGTCATGAC TAGTGCTTGG ATTCTCACCA ATAAAAAACG
     5401 CCCGGCGGCA ACCGAGCGTT CTGAACAAAT CCAGATGGAG TTCTGAGGTC ATTACTGGAT
     5461 CTATCAACAG GAGTCCAAGC GAGCTCGATA TCAAATTACG CCCCGCCCTG CCACTCATCG
     5521 CAGTACTGTT GTAATTCATT AAGCATTCTG CC
//