LOCUS       pAB1426_M50_Super_8x_TO 6320 bp ds-DNA     circular     14-MAR-2019
DEFINITION  .
KEYWORDS    "accession:addgene_12456_181170"                                   
SOURCE      synthetic DNA construct                                            
  ORGANISM  synthetic DNA construct                                            
FEATURES             Location/Qualifiers
     primer_bind     50..69
                     /label="Amp-R"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer          260..287
                     /label="PG0105"
                     /note="sequence: gggcgacacggaaatgttgaatactcat"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     promoter        complement(288..392)
                     /label="AmpR promoter"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     rep_origin      419..874
                     /label="f1 ori"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     primer          complement(460..478)
                     /label="PG0154"
                     /note="sequence: cggtcacgctgcgcgtaac"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer_bind     complement(506..525)
                     /label="F1ori-R"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer_bind     716..737
                     /label="F1ori-F"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     primer          842..863
                     /label="PG0292"
                     /note="sequence: cgaattttaacaaaatattaac"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer          885..906
                     /label="PG0185"
                     /note="sequence: ATTCAGGCTGCGCAACTGTTGG"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    1004..1023
                     /label="Lac-operon"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    1004..1026
                     /label="HindIII GH"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    complement(1027..1075)
                     /label="polyA signal"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     CDS             complement(1076..1078)
                     /label="Translation 1078-1076"
     source          complement(1076..3435)
                     /label="source"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     PCR_primer      1079..1106
                     /label="CTTTGCATTCATCTGGTACTTCTAGGGTG"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     gene            complement(1079..2737)
                     /label="CLuc"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     CDS             complement(1079..2737)
                     /label="Translation 2737-1079"
     primer          1106..1138
                     /label="PG0138"
                     /note="sequence: GTCTCCATGCTTTATGTAGCATTCTTTCTTGAA"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     Cut site        complement(1172..1178)
                     /label="Eco81I"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    complement(1758..1788)
                     /label="NGS R"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    complement(1797..1799)
                     /label="motif to edit"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    complement(1800..1802)
                     /label="motif to edit"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    complement(1822..1843)
                     /label="P3-F"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    complement(1832..1837)
                     /label="randomize these"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    complement(1835..1840)
                     /label="randomize these"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    complement(1845..1864)
                     /label="NGS F"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    2108..2137
                     /label="Guide opt 5"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    complement(2133..2139)
                     /label="Perfect overlap"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    complement(2133..2150)
                     /label="Limited overlap"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    complement(2159..2186)
                     /label="Limited overlap"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    2171..2200
                     /label="Guide opt 1"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    complement(2187..2211)
                     /label="R primer 2"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    complement(2192..2238)
                     /label="Limited overlap"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    2204..2225
                     /label="P1-R"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    2244..2273
                     /label="Guide opt 2"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    complement(2268..2304)
                     /label="Limited overlap"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    complement(2280..2301)
                     /label="P2-F"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    2346..2375
                     /label="Guide opt 3"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    complement(2355..2383)
                     /label="Limited overlap"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    complement(2405..2419)
                     /label="Limited overlap"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    2415..2444
                     /label="Guide opt 4"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    complement(2486..2505)
                     /label="F primer 2"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    complement(2612..2640)
                     /label="guide 6"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    complement(2615..2644)
                     /label="APOBEC stop 11"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    complement(2618..2646)
                     /label="guide 5"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    complement(2619..2648)
                     /label="APOBEC stop 10"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    complement(2623..2652)
                     /label="APOBEC stop 9"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    complement(2624..2652)
                     /label="guide 4"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    complement(2627..2656)
                     /label="APOBEC stop 8"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    complement(2631..2659)
                     /label="guide 3"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    complement(2631..2660)
                     /label="APOBEC stop 7"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    complement(2635..2664)
                     /label="APOBEC stop 6"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    complement(2638..2666)
                     /label="guide 2"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    complement(2639..2668)
                     /label="APOBEC stop 5"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    complement(2643..2672)
                     /label="APOBEC stop 4"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    complement(2646..2674)
                     /label="guide 1"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    complement(2647..2676)
                     /label="APOBEC stop 3"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    complement(2651..2680)
                     /label="APOBEC stop 2"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    complement(2677..2705)
                     /label="guide seed"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    complement(2681..2683)
                     /label="APOBEC site for making stop codon"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    complement(2684..2713)
                     /label="APOBEC stop 1"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    complement(2738..2743)
                     /label="Kozak"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     Cut Site        complement(2744..2749)
                     /label="KpnI"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     Cut site        complement(2750..2755)
                     /label="HindIII"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     primer_bind     complement(2762..2780)
                     /label="T7"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer          complement(2762..2781)
                     /label="T7"
                     /note="sequence: TAATACGACTCACTATAGGG"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    complement(2772..2780)
                     /label="F primer 1"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     primer_bind     complement(2781..2781)
                     /label="T7"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    complement(2781..2804)
                     /label="F primer 1"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     promoter        complement(2781..3435)
                     /label="PCMV"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     primer          complement(2855..2875)
                     /label="CMV-Forward"
                     /note="sequence: CGCAAATGGGCGGTAGGCGTG"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     primer          2961..2985
                     /label="PG0087"
                     /note="sequence: gagacttggaaatccccgtgagtca"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     primer          complement(3038..3069)
                     /label="PG0103"
                     /note="sequence: ttggcagtacatctacgtattagtcatcgcta"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     primer          3349..3388
                     /label="PG0181"
                     /note="sequence: gggctatgaactaatgaccccgtaattgattactattaat"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer          3365..3394
                     /label="PC1381"
                     /note="sequence: accccgtaattgattactattaataactag"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     primer          complement(3396..3435)
                     /label="PC1304"
                     /note="sequence: cgatgtacgggccagatatacgcgttgacattgattattg"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     primer          complement(3406..3435)
                     /label="PC2509"
                     /note="sequence: cgatgtacgggccagatatacgcgttgaca"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     polyA_signal    3436..3484
                     /label="synthetic polyadenylation signal"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    3498..3589
                     /label="pause site"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     primer_bind     3538..3557
                     /label="RVprimer3"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    3591..3615
                     /label="Gibson overhang"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     primer          complement(3591..3615)
                     /label="PC3409"
                     /note="sequence: cgcgtaagagctcggtacctatcga"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     source          join(3602..6320,1..3601)
                     /label="source"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     promoter        3757..3788
                     /label="minP"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     regulatory      3844..3849
                     /label="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    3850..4404
                     /label="G-luciferase"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     CDS             3850..4404
                     /label="Translation 3850-4404"
     misc_feature    complement(3852..3879)
                     /label="C2c2-1"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    complement(3852..3880)
                     /label="Cas13b-1"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     primer          3923..3943
                     /label="PC0538"
                     /note="sequence: ACTTCAACATCGTGGCCGTGG"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     primer          3923..3943
                     /label="PC0540"
                     /note="sequence: ACTTCAACATCGTGGCCGTGG"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     primer          complement(3944..3965)
                     /label="PC0555"
                     /note="sequence: TCCGTGGTCGCGAAGTTGCTGG"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer          complement(3944..3973)
                     /label="PC0568"
                     /note="sequence: CATCGAGATCCGTGGTCGCGAAGTTGCTGG"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     primer          complement(3981..4009)
                     /label="PC0247"
                     /note="sequence: GCGGCAGCTTCTTGCCGGGCAACTTCCCG"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    complement(3990..4009)
                     /label="Anneal"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     primer          complement(3995..4022)
                     /label="PC0250"
                     /note="sequence: TTGAGCACCTCCAGCGGCAGCTTCTTGC"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    complement(4002..4022)
                     /label="Anneal"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    complement(4006..4035)
                     /label="6-guide-2"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    complement(4010..4045)
                     /label="Semi degenerate oligo (b)"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    complement(4013..4042)
                     /label="Canonical-2"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    complement(4013..4042)
                     /label="15 guide 2"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    complement(4013..4042)
                     /label="Canonical guide 2"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    complement(4023..4056)
                     /label="Semi degenerate oligo (a/c)"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    complement(4026..4053)
                     /label="C2c2-1"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    complement(4040..4184)
                     /label="C-U Reporter mutagenesis site 1"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    complement(4046..4075)
                     /label="GH"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    complement(4057..4086)
                     /label="GH"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer          complement(4067..4091)
                     /label="PC0709"
                     /note="sequence: TTGATGTGGGACAGGCAGATCAGAC"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    complement(4145..4289)
                     /label="C-U Reporter mutagenesis site 2"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    complement(4166..4195)
                     /label="15 guide 5"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    complement(4166..4195)
                     /label="Canonical-5"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    complement(4167..4196)
                     /label="Canonical guide 5"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    complement(4183..4210)
                     /label="C2c2-2"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    4386..4404
                     /label="Gibson R"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     polyA_signal    4448..4569
                     /label="SV40 poly(A) signal"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     primer_bind     complement(4459..4478)
                     /label="EBV-rev"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     primer          4460..4489
                     /label="PG0191"
                     /note="sequence: ATGAGTTTGGACAAACCACAACTAGAATGC"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer_bind     4513..4532
                     /label="SV40pA-R"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     primer_bind     complement(4817..4834)
                     /label="L4440"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     primer          complement(4854..4872)
                     /label="PG0262"
                     /note="sequence: GAGCTGATACCGCTCGCCG"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     primer          4908..4927
                     /label="PG0047"
                     /note="sequence: ggggataacgcaggaaagaa"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     rep_origin      complement(4988..5576)
                     /label="ori"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     primer_bind     complement(5068..5087)
                     /label="pBR322ori-F"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     primer          complement(5305..5328)
                     /label="PG0166"
                     /note="sequence: GCGATAAGTCGTGTCTTACCGGGT"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     primer          complement(5336..5355)
                     /label="PG0039"
                     /note="sequence: tcctgttaccagtggctgct"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     primer          5601..5621
                     /label="PG0192"
                     /note="sequence: GGGTCTGACGCTCAGTGGAAC"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     primer          complement(5626..5645)
                     /label="PG0227"
                     /note="sequence: CAAAATCCCTTAACGTGAGT"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     CDS             complement(join(5747..6320,1..287))
                     /label="AmpR"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     primer          complement(6255..6281)
                     /label="PG0165"
                     /note="sequence: GCATCTTACGGATGGCATGACAGTAAG"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
ORIGIN
        1 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
       61 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
      121 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
      181 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
      241 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
      301 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
      361 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
      421 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
      481 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
      541 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
      601 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca
      661 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
      721 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa
      781 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
      841 gcgaatttta acaaaatatt aacgcttaca atttgccatt cgccattcag gctgcgcaac
      901 tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagcccaa gctaccatga
      961 taagtaagta atattaaggt acgggaggta cttggagcgg ccgcagctat gaccatgatt
     1021 acgccaCACA CAAAAAACCA ACACACAGAT GTAATGAAAA TAAAGATATT TTATTTTAtt
     1081 tgcattcatc tggtacttct agggtgtctc catgctttat gtagcattct ttcttgaact
     1141 cccatgcgtg gtcacagaaa ccctgttgtc ccctcaggca atactcgtac atgcatcgtt
     1201 cgacacggtc aggcttgtgg cacacgttac atttctgatc aagatcactt tgaccggcga
     1261 agagactatt gcagagtcgt tcagcttcag gtttctgttc ttcggtgcat cccggtgggt
     1321 tgggggtcag atcacaggct ccttcagcat caaaagaatc atcactgaaa tcctggttgt
     1381 agttaccgca aataccgcaa gtcttaccat cgaatggatc tctaatatgt acgacgagca
     1441 gttgcttgaa gttgaacttg accaccagag cttcaggtag gatggctgta gtcagtatgt
     1501 caccatcttg ccagtagatg gaagtgttct gagagctgta cgggacggac acggcttctc
     1561 ctccaaccag aatctgtttt ccatcaacaa tgagttctac tacagtcgat tgtttcctga
     1621 ttcgtacttt ctctacttca gtgtaagagt caacattcct gtgtgaaacc ttcacatccc
     1681 aagtgttcca gaaacagtcg gcggccataa gaatctcctt gcagggaccc tggaattggt
     1741 atcttgcttt gtcaaatgta tcgtagaaag tatgtccaga caaaacgcag gttcctctag
     1801 tttcgggagc agcgcacgtc tccctgtagt caagcagcac gtgtgaggct cgctcgtctc
     1861 cacccataca gcgggcgaag gcgcaggaga tggtgtagta gtagaagttg atggggttgc
     1921 ggcagctgtc cttgtacggc tccagaagac ctttgcagta tgcaacgtca tcaggatttc
     1981 catagagtgg acaaccgtca aactcctggt tgatcttagg ctgaatagcg agttgttctg
     2041 gatctgaagt gaagtctgta tcttccatca ttttaagatc tccacagagg ccagagatca
     2101 ttcctttgtt tgctgtgtct ggggcgattc ttacagatct tcctccgagg atgtcgatca
     2161 cgatcagttt gaagaactca atgacggtga tgttgaagcc tggcatctca acaacagcga
     2221 tggtgacctc gccgatggtg tacgggttgg cgatgatagg gtcagctcca ccgtttacag
     2281 tgatgggatt ctcagtagct tgagcgatgt caatgatgtc tccagccact tccagtcttg
     2341 tcttggtcag cacagccccc ttggttccat ccaggttctc cagggtgatg gacaccttcc
     2401 agtcgccgcc cttggttcct tgacccaaca cgtatgtacc aggttcctgg aactggaatc
     2461 tctttccata gaatgttcta aaccatcctg cggcctctac tctgcattca attacatact
     2521 gacacattcg ACAacatgtt tttcctggtt tattttcaca cagtccatct gatagtatgt
     2581 ctctcgtgca ggtgccacag ctgctatcaa tacattctcc ttctttagct tcacaggaag
     2641 ttggaactgt gtttggtgga tcaggttcgt aaggacagtc ctggcaatga acagtggcgc
     2701 agtagactaa tgcaacggca agaattaagg tcttcatggt ggcggtacca agcttgggtc
     2761 tccctatagt gagtcgtatt aatttcgata agccagtaag cagtgggttc tctagttagc
     2821 cagagagctc tgcttatata gacctcccac cgtacacgcc taccgcccat ttgcgtcaat
     2881 ggggcggagt tgttacgaca ttttggaaag tcccgttgat tttggtgcca aaacaaactc
     2941 ccattgacgt caatggggtg gagacttgga aatccccgtg agtcaaaccg ctatccacgc
     3001 ccattgatgt actgccaaaa ccgcatcacc atggtaatag cgatgactaa tacgtagatg
     3061 tactgccaag taggaaagtc ccataaggtc atgtactggg cataatgcca ggcgggccat
     3121 ttaccgtcat tgacgtcaat agggggcgta cttggcatat gatacacttg atgtactgcc
     3181 aagtgggcag tttaccgtaa atagtccacc cattgacgtc aatggaaagt ccctattggc
     3241 gttactatgg gaacatacgt cattattgac gtcaatgggc gggggtcgtt gggcggtcag
     3301 ccaggcgggc catttaccgt aagttatgta acgcggaact ccatatatgg gctatgaact
     3361 aatgaccccg taattgatta ctattaataa ctagtcaata atcaatgtca acgcgtatat
     3421 ctggcccgta catcgaataa aatatcttta ttttcattac atctgtgtgt tggttttttg
     3481 tgtgaatcga tagtactaac atacgctctc catcaaaaca aaacgaaaca aaacaaacta
     3541 gcaaaatagg ctgtccccag tgcaagtgca ggtgccagaa catttctcta tcgataggta
     3601 ccgagctctt acgcgagatc aaagggggta agatcaaagg gggtaagatc aaaggggcgc
     3661 gagatcaaag ggggtaagat caaagggggt aagatcaaag ggggtaagat caaaggggcg
     3721 cgcccgcgtg ctagcccggg ctcgagatct agactctaga gggtatataa tggaagctcg
     3781 aattccagct tggcattccg gtactgttgg taaaaagctt ggcattccgg tactgttggt
     3841 aaagccacca tgGGAGTCAA AGTTCTGTTT GCCCTGATCT GCATCGCTGT GGCCGAGGCC
     3901 AAGCCCACCG AGAACAACGA AGACTTCAAC ATCGTGGCCG TGGCCAGCAA CTTCGCGACC
     3961 ACGGATCTCG ATGCTGACCG CGGGAAGTTG CCCGGCAAGA AGCTGCCGCT GGAGGTGCTC
     4021 AAAGAGATGG AAGCCAATGC CCGGAAAGCT GGCTGCACCA GGGGCTGTCT GATCTGCCTG
     4081 TCCCACATCA AGTGCACGCC CAAGATGAAG AAGTTCATCC CAGGACGCTG CCACACCTAC
     4141 GAAGGCGACA AAGAGTCCGC ACAGGGCGGC ATAGGCGAGG CGATCGTCGA CATTCCTGAG
     4201 ATTCCTGGGT TCAAGGACTT GGAGCCCATG GAGCAGTTCA TCGCACAGGT CGATCTGTGT
     4261 GTGGACTGCA CAACTGGCTG CCTCAAAGGG CTTGCCAACG TGCAGTGTTC TGACCTGCTC
     4321 AAGAAGTGGC TGCCGCAACG CTGTGCGACC TTTGCCAGCA AGATCCAGGG CCAGGTGGAC
     4381 AAGATCAAGG GGGCCGGTGG TGACTAAttc tagagtcggg gcggccggcc gcttcgagca
     4441 gacatgataa gatacattga tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa
     4501 tgctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat
     4561 aaacaagtta acaacaacaa ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg
     4621 gaggtttttt aaagcaagta aaacctctac aaatgtggta aaatcgataa ggatccgtcg
     4681 accgatgccc ttgagagcct tcaacccagt cagctccttc cggtgggcgc ggggcatgac
     4741 tatcgtcgcc gcacttatga ctgtcttctt tatcatgcaa ctcgtaggac aggtgccggc
     4801 agcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
     4861 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     4921 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     4981 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     5041 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     5101 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     5161 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     5221 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     5281 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     5341 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     5401 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
     5461 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     5521 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     5581 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     5641 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     5701 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     5761 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     5821 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     5881 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     5941 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     6001 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     6061 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     6121 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     6181 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     6241 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
     6301 actcaaccaa gtcattctga
//