LOCUS       Exported                8682 bp DNA     circular SYN 03-JUN-2021
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8682)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 13, 2022 from SnapGene 6.0.0
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8682
                     /lab_host="Mammalian Cells"
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
                     /note="color: #ffffff"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
                     /note="color: #ffffff; direction: RIGHT"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /note="color: #ffffff; direction: RIGHT"
     regulatory      895..904
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /note="color: #a6acb3"
                     /regulatory_class="other"
     CDS             901..963
                     /codon_start=1
                     /label=His tag
                     /note="color: #008f00"
                     /translation="MGHHHHHHHHHHSSGHIEGRH"
     CDS             964..2244
                     /codon_start=1
                     /label=AKAP79
                     /note="color: #005493"
                     /translation="METTISEIHVENKDEKRSAEGSPGAERQKEKASMLCFKRRKKAAK
                     ALKPKAGSEAADVARKCPQEAGASDQPEPTRGAWASLKRLVTRRKRSESSKQQKPLEGE
                     MQPAINAEDADLSKKKAKSRLKIPCIKFPRGPKRSNHSKIIEDSDCSIKVQEEAEILDI
                     QTQTPLNDQATKAKSTQDLSEGISQKDGDEVCESNVSNSITSGEKVISVELGLDNGHSA
                     IQTGTLILEEIETIKEKQDVQPQQASPLETSETDHQQPVLSDVPPLPAIPDQQIVEEAS
                     NSTLESAPNGKDYESTEIVAEETKPKDTELSQESDFKENGITEEKSKSEESKRMEPIAI
                     IITDTEISEFDVTKSKNVPKQFLISAENEQVGVFANDNGFEDRTSEQYETLLIETASSL
                     VKNAIQLSIEQLVNEMASDDNKINNLLQ"
     CDS             2254..2970
                     /codon_start=1
                     /product="enhanced variant of YFP (Heikal et al., 2001)"
                     /label=Citrine
                     /note="mammalian codon-optimized"
                     /note="This feature has 3 segments:
                        1: 2254 .. 2256 / #e1ff2f
                        2: 2257 .. 2259 / #e1ff2f / 1a
                        3: 2260 .. 2970 / #e1ff2f"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             2977..3453
                     /codon_start=1
                     /label=Epac2B (285-443)
                     /note="color: #945200"
                     /translation="EEKKECDEELQDTMLLLSQMGPDAHMRMILRKPPGQRTVDDLEII
                     YDELLHIKALSHLSTTVKRELAGVLIFESHAKGGTVLFNQGEEGTSWYIILKGSVNVVI
                     YGKGVVCTLHEGDDFGKLALVNDAPRAASIVLREDNCHFLRVDKEDFNRILRDVE"
     CDS             3454..4170
                     /codon_start=1
                     /label=Cerulean
                     /note="color: #66ccff"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHRFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNGIK
                     AHFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     promoter        complement(4234..4252)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
                     /note="color: #ffffff; direction: LEFT"
     polyA_signal    4278..4502
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
                     /note="color: #a6acb3"
     rep_origin      4548..4976
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
     promoter        4990..5320
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
                     /note="color: #ffffff; direction: RIGHT"
     rep_origin      5171..5306
                     /label=SV40 ori
                     /note="SV40 origin of replication"
                     /note="color: #ffff00"
     CDS             5387..6181
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /note="color: #ccffcc"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    6355..6476
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
                     /note="color: #a6acb3"
     primer_bind     complement(6525..6541)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: LEFT"
     protein_bind    6549..6565
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #31849b"
     promoter        complement(6573..6603)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
                     /note="This reverse directional feature has 3 segments:
                        1: 6573 .. 6579 / #ffffff / -10
                        2: 6580 .. 6597 / #ffffff
                        3: 6598 .. 6603 / #ffffff / -35"
     protein_bind    6618..6639
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
                     /note="color: #31849b"
     rep_origin      complement(6927..7515)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     CDS             complement(7686..8546)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 7686 .. 8477 / #ccffcc
                        2: 8478 .. 8546 / #ccffcc / signal sequence
                     Cleavage site after base 8477"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(8547..8651)
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: LEFT"
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttgccacc
      901 atgggccatc atcatcatca tcatcatcat catcacagca gcggccatat cgaaggtcgt
      961 catatggaaa ccacaatttc agaaattcat gtagaaaaca aggatgagaa gagatcagca
     1021 gaaggtagtc ctggggctga aaggcagaag gaaaaggcat ccatgctttg cttcaagaga
     1081 agaaagaaag cagccaaagc actgaagccc aaagctggct ctgaagctgc tgatgtggca
     1141 aggaagtgtc cacaagaagc aggagcttct gatcagccag agcccacacg gggggcctgg
     1201 gcctcactca aacgtcttgt aacacgcagg aaaaggtcag agtcttcaaa gcagcaaaag
     1261 ccattggagg gtgaaatgca acctgcaata aatgctgagg atgctgatct ttctaagaaa
     1321 aaggcaaaat ctagacttaa gattccctgc ataaaattcc caagagggcc aaaaaggagt
     1381 aatcattcca aaattataga agactcagac tgcagcatca aagtccagga agaagctgaa
     1441 attttggata tacaaacaca gaccccattg aatgatcagg caacaaaggc taagtcaacc
     1501 caggatctaa gtgaaggcat ctcacagaaa gatggtgatg aggtctgtga atcaaatgtg
     1561 agcaatagca taacttctgg agagaaagtg atttcagtag aacttggatt agataatggg
     1621 cattctgcta ttcaaacggg aactctaatc cttgaagaaa ttgaaacgat caaggaaaaa
     1681 caagatgttc aaccccagca agcaagccca cttgaaactt cagaaacaga ccatcagcag
     1741 ccagtacttt ctgatgttcc tcctttacct gcaattccag atcaacaaat tgtggaagaa
     1801 gccagtaaca gtaccctaga aagtgcacca aatggaaaag actatgaaag tacagagatt
     1861 gtagctgaag aaactaagcc aaaagatact gaattgagcc aagaatcaga ttttaaagaa
     1921 aatgggatca ctgaagagaa atccaaatca gaagaaagca aaagaatgga gccaattgct
     1981 attattatta cagacactga aatcagtgaa tttgatgtta caaaatctaa aaatgtccct
     2041 aagcaattct taatttcagc tgaaaatgag caagtagggg tttttgctaa tgataatggt
     2101 tttgaggata gaacttcaga acaatatgaa acactcttaa ttgaaacagc ctcttctcta
     2161 gtcaagaatg ctattcagtt gtcaatagaa cagctggtta atgaaatggc ctctgatgat
     2221 aataaaataa acaatcttct acagaaggat cccatggtga gcaagggcga ggagctgttc
     2281 accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc
     2341 gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc
     2401 accaccggca agctgcccgt gccctggccc accctcgtga ccaccttcgg ctacggcctg
     2461 atgtgcttcg cccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg
     2521 cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc
     2581 cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc
     2641 gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac
     2701 aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt caagatccgc
     2761 cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc
     2821 ggcgacggcc ccgtgctgct gcccgacaac cactacctga gctaccagtc cgccctgagc
     2881 aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg
     2941 atcactctcg gcatggacga gctgtacaag gagttcgagg agaagaagga gtgtgatgaa
     3001 gaacttcagg acaccatgct gctgctctca cagatgggcc ctgacgccca catgagaatg
     3061 atcctgcgaa aaccacctgg ccagaggact gtggatgacc tagagattat ctacgatgag
     3121 ctccttcata ttaaagcctt atcccatctc tctaccacag tgaaacggga gttagcaggt
     3181 gttctcattt ttgagtctca cgccaaagga ggaactgtgt tgtttaacca gggggaagaa
     3241 ggaacctcct ggtacatcat tctgaaagga tccgtgaatg tagtcattta tggcaagggt
     3301 gtggtctgca ccctgcacga aggagatgac tttggcaagt tagctctagt gaacgatgct
     3361 ccaagagctg cctccattgt tcttcgggaa gataattgtc acttcctaag agtcgacaag
     3421 gaagacttca atcggatttt gagggacgtt gagatggtga gcaagggcga ggagctgttc
     3481 accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca caggttcagc
     3541 gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc
     3601 accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac ctggggcgtg
     3661 cagtgcttcg cccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg
     3721 cccgaaggct acgtccagga gcgtaccatc ttcttcaagg acgacggcaa ctacaagacc
     3781 cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc
     3841 gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacgc catcagtgac
     3901 aacgtctata tcaccgccga caagcagaag aacggcatca aggcccactt caagatccgc
     3961 cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc
     4021 ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc cgccctgagc
     4081 aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg
     4141 atcactctcg gcatggacga gctgtacaag taagaattct gcagatatcc atcacactgg
     4201 cggccgctcg agcatgcatc tagagggccc tattctatag tgtcacctaa atgctagagc
     4261 tcgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc
     4321 cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga
     4381 aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga
     4441 cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat
     4501 ggcttctgag gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag
     4561 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
     4621 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
     4681 tccccgtcaa gctctaaatc ggggcatccc tttagggttc cgatttagtg ctttacggca
     4741 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata
     4801 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
     4861 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttggg
     4921 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattaatt
     4981 ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagg caggcagaag
     5041 tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc
     5101 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct
     5161 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
     5221 actaattttt tttatttatg cagaggccga ggccgcctct gcctctgagc tattccagaa
     5281 gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctcccgg gagcttgtat
     5341 atccattttc ggatctgatc aagagacagg atgaggatcg tttcgcatga ttgaacaaga
     5401 tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc
     5461 acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc
     5521 ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcagg acgaggcagc
     5581 gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac
     5641 tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc
     5701 tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac
     5761 gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg
     5821 tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct
     5881 cgcgccagcc gaactgttcg ccaggctcaa ggcgcgcatg cccgacggcg aggatctcgt
     5941 cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg
     6001 attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac
     6061 ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg
     6121 tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg
     6181 agcgggactc tggggttcga aatgaccgac caagcgacgc ccaacctgcc atcacgagat
     6241 ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc
     6301 ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca ccccaacttg
     6361 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
     6421 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
     6481 gtctgtatac cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct
     6541 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
     6601 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
     6661 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
     6721 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     6781 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     6841 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     6901 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     6961 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     7021 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     7081 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc aatgctcacg ctgtaggtat
     7141 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     7201 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     7261 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     7321 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt
     7381 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     7441 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     7501 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     7561 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     7621 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     7681 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     7741 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     7801 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     7861 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     7921 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     7981 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     8041 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     8101 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     8161 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     8221 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     8281 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     8341 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     8401 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     8461 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     8521 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     8581 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     8641 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tc
//
