LOCUS       Exported                8739 bp DNA     circular SYN 03-JUN-2021
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 8739)
  AUTHORS   Sohum Mehta
  TITLE     Direct Submission
  JOURNAL   Exported Jan 13, 2022 from SnapGene 6.0.0
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8739
                     /lab_host="Mammalian Cells"
                     /mol_type="other DNA"
                     /organism="recombinant plasmid"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
                     /note="color: #ffffff"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
                     /note="color: #ffffff; direction: RIGHT"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /note="color: #ffffff; direction: RIGHT"
     regulatory      895..904
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /note="color: #a6acb3"
                     /regulatory_class="other"
     CDS             901..963
                     /codon_start=1
                     /label=His tag
                     /note="color: #008f00"
                     /translation="MGHHHHHHHHHHSSGHIEGRH"
     CDS             964..2244
                     /codon_start=1
                     /label=AKAP79
                     /note="color: #005493"
                     /translation="METTISEIHVENKDEKRSAEGSPGAERQKEKASMLCFKRRKKAAK
                     ALKPKAGSEAADVARKCPQEAGASDQPEPTRGAWASLKRLVTRRKRSESSKQQKPLEGE
                     MQPAINAEDADLSKKKAKSRLKIPCIKFPRGPKRSNHSKIIEDSDCSIKVQEEAEILDI
                     QTQTPLNDQATKAKSTQDLSEGISQKDGDEVCESNVSNSITSGEKVISVELGLDNGHSA
                     IQTGTLILEEIETIKEKQDVQPQQASPLETSETDHQQPVLSDVPPLPAIPDQQIVEEAS
                     NSTLESAPNGKDYESTEIVAEETKPKDTELSQESDFKENGITEEKSKSEESKRMEPIAI
                     IITDTEISEFDVTKSKNVPKQFLISAENEQVGVFANDNGFEDRTSEQYETLLIETASSL
                     VKNAIQLSIEQLVNEMASDDNKINNLLQ"
     CDS             2254..2970
                     /codon_start=1
                     /label=Cerulean
                     /note="color: #66ccff"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHRFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNGIK
                     AHFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             2980..3402
                     /codon_start=1
                     /product="KFSQEQIGENIVCRVICTTGQIPIRDLSADISQVLKEKRSIKKVWTFGR
                     NPACDYHLGNISRLSNKHFQILLGEDGNLLLNDISTNGTWLNGQKVEKNSNQLLSQGDE
                     ITVGVGVESDILSLVIFINDKFKQCLEQNKVDR"
                     /label=FHA1
                     /note="color: #804002"
                     /translation="KFSQEQIGENIVCRVICTTGQIPIRDLSADISQVLKEKRSIKKVW
                     TFGRNPACDYHLGNISRLSNKHFQILLGEDGNLLLNDISTNGTWLNGQKVEKNSNQLLS
                     QGDEITVGVGVESDILSLVIFINDKFKQCLEQNKVDR"
     CDS             3445..3468
                     /codon_start=1
                     /product="LRRATLVD"
                     /label=PKA substrate
                     /note="color: #666666"
                     /translation="LRRATLVD"
     CDS             3493..4227
                     /codon_start=1
                     /label=cpVenus(E172)
                     /note="color: #ffff0a"
                     /translation="MGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDH
                     MVLLEFVTAAGITLGMDELYKGGTGGSMVSKGEELFTGVVPILVELDGDVNGHKFSVSG
                     EGEGDATYGKLTLKLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPE
                     GYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
                     VYITADKQKNGIKANFKIRHNIE"
     promoter        complement(4291..4309)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
                     /note="color: #ffffff; direction: LEFT"
     polyA_signal    4335..4559
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
                     /note="color: #a6acb3"
     rep_origin      4605..5033
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
     promoter        5047..5377
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
                     /note="color: #ffffff; direction: RIGHT"
     rep_origin      5228..5363
                     /label=SV40 ori
                     /note="SV40 origin of replication"
                     /note="color: #ffff00"
     CDS             5444..6238
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /note="color: #ccffcc"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    6412..6533
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
                     /note="color: #a6acb3"
     primer_bind     complement(6582..6598)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: LEFT"
     protein_bind    6606..6622
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #31849b"
     promoter        complement(6630..6660)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
                     /note="This reverse directional feature has 3 segments:
                        1: 6630 .. 6636 / #ffffff / -10
                        2: 6637 .. 6654 / #ffffff
                        3: 6655 .. 6660 / #ffffff / -35"
     protein_bind    6675..6696
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
                     /note="color: #31849b"
     rep_origin      complement(6984..7572)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     CDS             complement(7743..8603)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 7743 .. 8534 / #ccffcc
                        2: 8535 .. 8603 / #ccffcc / signal sequence
                     Cleavage site after base 8534"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(8604..8708)
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: LEFT"
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttgccacc
      901 atgggccatc atcatcatca tcatcatcat catcacagca gcggccatat cgaaggtcgt
      961 catatggaaa ccacaatttc agaaattcat gtagaaaaca aggatgagaa gagatcagca
     1021 gaaggtagtc ctggggctga aaggcagaag gaaaaggcat ccatgctttg cttcaagaga
     1081 agaaagaaag cagccaaagc actgaagccc aaagctggct ctgaagctgc tgatgtggca
     1141 aggaagtgtc cacaagaagc aggagcttct gatcagccag agcccacacg gggggcctgg
     1201 gcctcactca aacgtcttgt aacacgcagg aaaaggtcag agtcttcaaa gcagcaaaag
     1261 ccattggagg gtgaaatgca acctgcaata aatgctgagg atgctgatct ttctaagaaa
     1321 aaggcaaaat ctagacttaa gattccctgc ataaaattcc caagagggcc aaaaaggagt
     1381 aatcattcca aaattataga agactcagac tgcagcatca aagtccagga agaagctgaa
     1441 attttggata tacaaacaca gaccccattg aatgatcagg caacaaaggc taagtcaacc
     1501 caggatctaa gtgaaggcat ctcacagaaa gatggtgatg aggtctgtga atcaaatgtg
     1561 agcaatagca taacttctgg agagaaagtg atttcagtag aacttggatt agataatggg
     1621 cattctgcta ttcaaacggg aactctaatc cttgaagaaa ttgaaacgat caaggaaaaa
     1681 caagatgttc aaccccagca agcaagccca cttgaaactt cagaaacaga ccatcagcag
     1741 ccagtacttt ctgatgttcc tcctttacct gcaattccag atcaacaaat tgtggaagaa
     1801 gccagtaaca gtaccctaga aagtgcacca aatggaaaag actatgaaag tacagagatt
     1861 gtagctgaag aaactaagcc aaaagatact gaattgagcc aagaatcaga ttttaaagaa
     1921 aatgggatca ctgaagagaa atccaaatca gaagaaagca aaagaatgga gccaattgct
     1981 attattatta cagacactga aatcagtgaa tttgatgtta caaaatctaa aaatgtccct
     2041 aagcaattct taatttcagc tgaaaatgag caagtagggg tttttgctaa tgataatggt
     2101 tttgaggata gaacttcaga acaatatgaa acactcttaa ttgaaacagc ctcttctcta
     2161 gtcaagaatg ctattcagtt gtcaatagaa cagctggtta atgaaatggc ctctgatgat
     2221 aataaaataa acaatcttct acagaaggat cccatggtga gcaagggcga ggagctgttc
     2281 accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca caggttcagc
     2341 gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc
     2401 accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac ctggggcgtg
     2461 cagtgcttcg cccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg
     2521 cccgaaggct acgtccagga gcgtaccatc ttcttcaagg acgacggcaa ctacaagacc
     2581 cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc
     2641 gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacgc catcagtgac
     2701 aacgtctata tcaccgccga caagcagaag aacggcatca aggcccactt caagatccgc
     2761 cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc
     2821 ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc cgccctgagc
     2881 aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg
     2941 atcactctcg gcatggacga gctgtacaag cgcatgcata agttttctca agaacagatc
     3001 ggcgaaaaca ttgtgtgcag ggtcatttgt accacgggtc aaattcccat ccgagatttg
     3061 tcagctgata tttcacaagt gcttaaggaa aaacgatcca taaagaaagt ttggacattt
     3121 ggtagaaacc cagcctgtga ctatcattta ggaaacattt caagactgtc aaataagcat
     3181 ttccaaatac tactaggaga agacggtaac cttttattga atgacatttc cactaatggg
     3241 acctggttaa atgggcaaaa agtcgagaag aacagcaatc agttactgtc tcaaggtgat
     3301 gaaataaccg ttggtgtagg cgtggaatca gatattttat ctctggtcat tttcataaac
     3361 gacaaattta agcagtgcct cgagcagaac aaagttgatc gctctgcagg taagccaggc
     3421 agcggcgagg gcagcaccaa gggcctgcgt cgcgccaccc tggtggacgg cggcaccggc
     3481 ggcagcgagc tcatgggcgg cgtgcagctc gccgaccact accagcagaa cacccccatc
     3541 ggcgacggcc ccgtgctgct gccngacaac cactacctga gctaccagtc caagctgagc
     3601 aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg
     3661 atcactctcg gcatggacga gctgtacaag ggaggtaccg gtggatctat ggtgagcaag
     3721 ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac
     3781 ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc
     3841 ctgaagctga tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc
     3901 ctgggctacg gccttcagtg cttcgcccgc taccccgacc acatgaagca gcacgacttc
     3961 ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac
     4021 ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc
     4081 gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac
     4141 aactacaaca gccacaacgt ctatatcacc gccgacaagc agaagaacgg catcaaggcc
     4201 aacttcaaga tccgccacaa catcgagtaa gaattctgca gatatccatc acactggcgg
     4261 ccgctcgagc atgcatctag agggccctat tctatagtgt cacctaaatg ctagagctcg
     4321 ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt
     4381 gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat
     4441 tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag
     4501 caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggc
     4561 ttctgaggcg gaaagaacca gctggggctc tagggggtat ccccacgcgc cctgtagcgg
     4621 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc
     4681 cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc
     4741 ccgtcaagct ctaaatcggg gcatcccttt agggttccga tttagtgctt tacggcacct
     4801 cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac
     4861 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac
     4921 tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttggggat
     4981 ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attaattctg
     5041 tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccaggcag gcagaagtat
     5101 gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc
     5161 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac
     5221 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
     5281 aatttttttt atttatgcag aggccgaggc cgcctctgcc tctgagctat tccagaagta
     5341 gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc
     5401 cattttcgga tctgatcaag agacaggatg aggatcgttt cgcatgattg aacaagatgg
     5461 attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca
     5521 acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt
     5581 tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaggacg aggcagcgcg
     5641 gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga
     5701 agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca
     5761 ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct
     5821 tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac
     5881 tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc
     5941 gccagccgaa ctgttcgcca ggctcaaggc gcgcatgccc gacggcgagg atctcgtcgt
     6001 gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt
     6061 catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg
     6121 tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat
     6181 cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc
     6241 gggactctgg ggttcgaaat gaccgaccaa gcgacgccca acctgccatc acgagatttc
     6301 gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc
     6361 tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc caacttgttt
     6421 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
     6481 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc
     6541 tgtataccgt cgacctctag ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg
     6601 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa
     6661 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
     6721 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
     6781 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
     6841 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     6901 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     6961 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     7021 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     7081 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     7141 tccgcctttc tcccttcggg aagcgtggcg ctttctcaat gctcacgctg taggtatctc
     7201 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     7261 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     7321 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     7381 acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc
     7441 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     7501 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     7561 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     7621 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     7681 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     7741 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     7801 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     7861 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
     7921 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     7981 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     8041 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     8101 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     8161 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     8221 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     8281 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     8341 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
     8401 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
     8461 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
     8521 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
     8581 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
     8641 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     8701 gttccgcgca catttccccg aaaagtgcca cctgacgtc
//
