LOCUS       Exported                9687 bp DNA     circular SYN 14-JAN-2022
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9687)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 14, 2022 from SnapGene Viewer 6.0.0
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9687
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
                     /note="color: #ffffff"
     misc_feature    405..411
                     /label=misc_feature 1
                     /note="color: #50ff50"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
                     /note="color: #ffffff; direction: RIGHT"
     primer_bind     621..640
                     /label=HKO084
                     /note="HKO084"
                     /note="color: #ff8080; direction: RIGHT"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /note="color: #ffe4c4"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
                     /note="color: #ffe4c4"
     misc_feature    1124..1127
                     /label=SD; splice donor
                     /note="SD; splice donor"
                     /note="color: #ff8000"
     misc_feature    1680..1913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
                     /note="color: #ffe4c4"
     CDS             2098..2142
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /note="color: #cc99b2"
                     /translation="KNEQELLELDKWASL"
     primer_bind     2408..2427
                     /label=HKO074
                     /note="HKO074"
                     /note="color: #ff8080; direction: RIGHT"
     misc_feature    2440..2557
                     /label=CPPT; central polypurine tract
                     /note="CPPT; central polypurine tract"
                     /note="color: #ff8000"
     promoter        2608..2863
                     /label=EFS-NS
                     /note="EFS-NS"
                     /note="color: #8080ff"
     promoter        2651..2862
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
                     /note="color: #ffffff; direction: RIGHT"
     misc_feature    2841..2884
                     /label=Forward (2841..2887)
                     /note="color: #50ff50"
     misc_feature    2870..2900
                     /label=Forward (2870..2900)
                     /note="color: #50ff50"
     misc_feature    2885..3690
                     /label=tNGFR
                     /note="color: #ff8000"
     misc_feature    2885..2944
                     /label=signal
                     /note="signal"
                     /note="color: #ff8000"
     misc_feature    2945..3610
                     /label=extracellular
                     /note="extracellular"
                     /note="color: #ff8000"
     misc_feature    3152..3153
                     /label=in colony 2, CCA mutated to CCG
                     /note="in colony 2, CCA mutated to CCG (P->P)"
                     /note="color: #ff8000"
     misc_feature    3214
                     /label=G->C
                     /note="G->C"
                     /note="color: #ff8000"
     misc_feature    3611..3676
                     /label=TM
                     /note="TM"
                     /note="color: #ff8000"
     misc_feature    complement(3671..3691)
                     /label=Reverse (3671..3691)
                     /note="color: #50ff50; direction: LEFT"
     misc_feature    complement(3672..3706)
                     /label=Reverse (3672..3706)
                     /note="color: #50ff50; direction: LEFT"
     misc_feature    3692..3757
                     /label=P2A
                     /note="color: #ff8000"
     misc_feature    complement(3692..3721)
                     /label=Reverse (4079..4108)
                     /note="color: #50ff50; direction: LEFT"
     misc_feature    3692..3707
                     /label=Forward (4079..4094)
                     /note="color: #50ff50"
     CDS             3701..3757
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /note="color: #cc99b2
                     Cleavage site after base 3754"
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             3758..4354
                     /label=Puro
                     /note="color: #0000ff; direction: RIGHT"
     misc_feature    complement(4361..4385)
                     /label=Reverse (4748..4772)
                     /note="color: #50ff50; direction: LEFT"
     misc_feature    4377..4970
                     /label=WPRE; Woodchuck post-transcriptional
                     /note="WPRE; Woodchuck post-transcriptional regulatory 
                     element"
                     /note="color: #ff8000"
     misc_feature    complement(4437..4456)
                     /label=misc_feature 2
                     /note="color: #50ff50; direction: LEFT"
     primer_bind     4458..4477
                     /label=HKO083
                     /note="HKO083"
                     /note="color: #ff8080; direction: RIGHT"
     CDS             complement(4850..4861)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /note="color: #cc99b2
                     Cleavage site after base 4849"
                     /translation="IEGR"
     primer_bind     complement(4970..4986)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: LEFT"
     primer_bind     5230..5250
                     /label=HKO085
                     /note="HKO085"
                     /note="color: #ff8080; direction: RIGHT"
     LTR             5492..5672
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /note="color: #ffe4c4"
     polyA_signal    5704..5928
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
                     /note="color: #a6acb3"
     rep_origin      5974..6402
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
     promoter        6416..6745
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
                     /note="color: #ffffff; direction: RIGHT"
     rep_origin      6596..6731
                     /label=SV40 ori
                     /note="SV40 origin of replication"
                     /note="color: #ffff00"
     promoter        6793..6840
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
                     /note="color: #ffffff; direction: RIGHT"
     CDS             6859..7233
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /note="color: #ccffcc"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    7363..7484
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
                     /note="color: #a6acb3"
     primer_bind     complement(7533..7549)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: LEFT"
     protein_bind    7557..7573
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #31849b"
     promoter        complement(7581..7611)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
                     /note="This reverse directional feature has 3 segments:
                        1: 7581 .. 7587 / #ffffff / -10
                        2: 7588 .. 7605 / #ffffff
                        3: 7606 .. 7611 / #ffffff / -35"
     protein_bind    7626..7647
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
                     /note="color: #31849b"
     rep_origin      complement(7935..8523)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     rep_origin      8122..8347
                     /direction=RIGHT
                     /label=ColE1
                     /note="ColE1"
                     /note="color: #c0c0c0"
     CDS             complement(8694..9554)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 8694 .. 9485 / #ccffcc
                        2: 9486 .. 9554 / #ccffcc / signal sequence
                     Cleavage site after base 9485"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(9555..9659)
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: LEFT"
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaatta agctagctag gtcttgaaag gagtgggaat tggctccggt
     2641 gcccgtcagt gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc
     2701 ggcaattgat ccggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg
     2761 tactggctcc gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc
     2821 gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac aggaccggtt ctagagccgc
     2881 caccatgtac gacgggccgc gcctgctgct gttgctgctt ctgggggtgt cccttggagg
     2941 tgccaaggag gcatgcccca caggcctgta cacacacagc ggtgagtgct gcaaagcctg
     3001 caacctgggc gagggtgtgg cccagccttg tggagccaac cagaccgtgt gtgagccctg
     3061 cctggacagc gtgacgttct ccgacgtggt gagcgcgacc gagccgtgca agccgtgcac
     3121 cgagtgcgtg gggctccaga gcatgtcggc gccgtgcgtg gaggccgacg acgccgtgtg
     3181 ccgctgcgcc tacggctact accaggatga gaccactggg cgctgcgagg cgtgccgcgt
     3241 gtgcgaggcg ggctcgggcc tcgtgttctc ctgccaggac aagcagaaca ccgtgtgcga
     3301 ggagtgcccc gacggcacgt attccgacga ggccaaccac gtggacccgt gcctgccctg
     3361 caccgtgtgc gaggacaccg agcgccagct ccgcgagtgc acacgctggg ccgacgccga
     3421 gtgcgaggag atccctggcc gttggattac acggtccaca cccccagagg gctcggacag
     3481 cacagccccc agcacccagg agcctgaggc acctccagaa caagacctca tagccagcac
     3541 ggtggcaggt gtggtgacca cagtgatggg cagctcccag cccgtggtga cccgaggcac
     3601 caccgacaac ctcatccctg tctattgctc catcctggct gctgtggttg tgggtcttgt
     3661 ggcctacata gccttcaaga ggtggaacag tggatccggc gcaacaaact tctctctgct
     3721 gaaacaagcc ggagatgtcg aagagaatcc tggaccgacc gagtacaagc ccacggtgcg
     3781 cctcgccacc cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg cgttcgccga
     3841 ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct
     3901 gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga
     3961 cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc
     4021 cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc agcaacagat
     4081 ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg
     4141 agtctcgccc gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga
     4201 ggcggccgag cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc gcaacctccc
     4261 cttctacgag cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg
     4321 cacctggtgc atgacccgca agcccggtgc ctgagaattc gatatcaagc ttatcggtaa
     4381 tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc
     4441 ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat
     4501 ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg
     4561 gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg
     4621 ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat
     4681 tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt
     4741 gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc
     4801 ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa
     4861 tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg
     4921 ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcatc gataccgtcg
     4981 acctcgagac ctagaaaaac atggagcaat cacaagtagc aatacagcag ctaccaatgc
     5041 tgattgtgcc tggctagaag cacaagagga ggaggaggtg ggttttccag tcacacctca
     5101 ggtaccttta agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga
     5161 aaagggggga ctggaagggc taattcactc ccaacgaaga caagatatcc ttgatctgtg
     5221 gatctaccac acacaaggct acttccctga ttggcagaac tacacaccag ggccagggat
     5281 cagatatcca ctgacctttg gatggtgcta caagctagta ccagttgagc aagagaaggt
     5341 agaagaagcc aatgaaggag agaacacccg cttgttacac cctgtgagcc tgcatgggat
     5401 ggatgacccg gagagagaag tattagagtg gaggtttgac agccgcctag catttcatca
     5461 catggcccga gagctgcatc cggactgtac tgggtctctc tggttagacc agatctgagc
     5521 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg
     5581 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag
     5641 acccttttag tcagtgtgga aaatctctag cagggcccgt ttaaacccgc tgatcagcct
     5701 cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga
     5761 ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt
     5821 gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg
     5881 attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg
     5941 aaagaaccag ctggggctct agggggtatc cccacgcgcc ctgtagcggc gcattaagcg
     6001 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg
     6061 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc
     6121 taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa
     6181 aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc
     6241 ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac
     6301 tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt
     6361 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg
     6421 tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
     6481 tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     6541 gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc
     6601 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
     6661 ttatgcagag gccgaggccg cctctgcctc tgagctattc cagaagtagt gaggaggctt
     6721 ttttggaggc ctaggctttt gcaaaaagct cccgggagct tgtatatcca ttttcggatc
     6781 tgatcagcac gtgttgacaa ttaatcatcg gcatagtata tcggcatagt ataatacgac
     6841 aaggtgagga actaaaccat ggccaagttg accagtgccg ttccggtgct caccgcgcgc
     6901 gacgtcgccg gagcggtcga gttctggacc gaccggctcg ggttctcccg ggacttcgtg
     6961 gaggacgact tcgccggtgt ggtccgggac gacgtgaccc tgttcatcag cgcggtccag
     7021 gaccaggtgg tgccggacaa caccctggcc tgggtgtggg tgcgcggcct ggacgagctg
     7081 tacgccgagt ggtcggaggt cgtgtccacg aacttccggg acgcctccgg gccggccatg
     7141 accgagatcg gcgagcagcc gtgggggcgg gagttcgccc tgcgcgaccc ggccggcaac
     7201 tgcgtgcact tcgtggccga ggagcaggac tgacacgtgc tacgagattt cgattccacc
     7261 gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc
     7321 ctccagcgcg gggatctcat gctggagttc ttcgcccacc ccaacttgtt tattgcagct
     7381 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
     7441 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctgtataccg
     7501 tcgacctcta gctagagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt
     7561 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt
     7621 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg
     7681 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg
     7741 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
     7801 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     7861 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     7921 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     7981 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     8041 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     8101 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     8161 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     8221 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     8281 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     8341 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
     8401 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     8461 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     8521 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     8581 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     8641 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     8701 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     8761 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
     8821 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     8881 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     8941 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     9001 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
     9061 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
     9121 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
     9181 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
     9241 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
     9301 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
     9361 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
     9421 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
     9481 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
     9541 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
     9601 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
     9661 acatttcccc gaaaagtgcc acctgac
//
