LOCUS       Exported                8722 bp DNA     circular SYN 14-JAN-2022
DEFINITION  Ligation of RUNX1A* into DHV020 - pRRL.PPT.SFFV.MCS.iGFP.pre# 
            SnaBI**.
ACCESSION   .
VERSION     .
KEYWORDS    pRRL.PPT.SFFV.RUNX1A.iGFP.pre
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8722)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 14, 2022 from SnapGene Viewer 6.0.0
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8722
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    4..235
                     /label=RSV
                     /note="color: #cc0000; direction: RIGHT"
     misc_feature    236..417
                     /label=RU5
                     /note="color: #cc0000; direction: RIGHT"
     LTR             236..416
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /note="color: #ffe4c4"
     misc_feature    418..568
                     /label=PBS
                     /note="color: #cc0000; direction: RIGHT"
     misc_feature    460..585
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
                     /note="color: #ffe4c4"
     misc_feature    569..933
                     /label=dGag
                     /note="color: #cc0000; direction: RIGHT"
     misc_feature    1078..1311
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
                     /note="color: #ffe4c4"
     misc_feature    1079..1320
                     /label=RRE
                     /note="color: #cc0000; direction: RIGHT"
     CDS             1496..1540
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /note="color: #cc99b2"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1804..1927
                     /label=PPT
                     /note="color: #cc0000; direction: RIGHT"
     misc_feature    1807..1924
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
                     /note="color: #ffe4c4"
     misc_feature    1947..2347
                     /label=SFFV
                     /note="color: #cc0000; direction: RIGHT"
     promoter        1958..2364
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
                     /note="color: #ffffff; direction: RIGHT"
     regulatory      2405..2414
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /note="color: #a6acb3"
                     /regulatory_class="other"
     CDS             2411..3190
                     /codon_start=1
                     /label=RUNX1A
                     /note="color: #cc0000"
                     /translation="MGYPYDVPDYRIPVDASTSRRFTPPSTALSPGKMSEALPLGAPDA
                     GAALAGKLRSGDRSMVEVLADHPGELVRTDSPNFLCSVLPTHWRCNKTLPIAFKVVALG
                     DVPDGTLVTVMAGNDENYSAELRNATAAMKNQVARFNDLRFVGRSGRGKSFTLTITVFT
                     NPPQVATYHRAIKITVDGPREPRRHRQKLDDQTKPGSLSFSERLSELEQLRRTAMRVSP
                     HHPAPTPNPRASLNHSTAFNPQPQSQMQEEDTAPWRC"
     misc_feature    2414..2440
                     /label=HA
                     /note="color: #cc0000; direction: RIGHT"
     misc_feature    3016..3039
                     /label=SK_RUNX1.1
                     /note="as, mutated"
                     /note="color: #cc0000; direction: RIGHT"
     misc_feature    3192..3202
                     /label=''MCS
                     /note="color: #cc0000; direction: RIGHT"
     CDS             3202..3204
                     /codon_start=1
                     /label=STOP
                     /note="color: #cc0000"
                     /translation=""
     CDS             3206..3208
                     /codon_start=1
                     /label=STOP
                     /note="color: #cc0000"
                     /translation=""
     CDS             3210..3212
                     /codon_start=1
                     /label=STOP
                     /note="color: #cc0000"
                     /translation=""
     misc_feature    3219..3803
                     /label=IRES
                     /note="color: #cc0000; direction: RIGHT"
     misc_feature    3220..3806
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
                     /note="This feature has 3 segments:
                        1: 3220 .. 3794 / #a6acb3
                        2: 3795 .. 3797 / #a6acb3 / ATG
                        3: 3798 .. 3806 / #a6acb3"
     CDS             3795..4516
                     /codon_start=1
                     /label=EGFP'
                     /note="color: #cc0000"
                     /translation="MATTMVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYG
                     KLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
                     FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQK
                     NGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH
                     MVLLEFVTAAGITLGMDE"
     CDS             3807..4526
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /note="This feature has 3 segments:
                        1: 3807 .. 3809 / #05fd14
                        2: 3810 .. 3812 / #05fd14 / 1a
                        3: 3813 .. 4526 / #05fd14"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             4518..4526
                     /codon_start=1
                     /label='EGFP
                     /note="color: #cc0000"
                     /translation="YK"
     misc_feature    4565..5167
                     /label=PRE*
                     /note="color: #cc0000; direction: RIGHT"
     misc_feature    4758..5161
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
                     /note="color: #ffffff"
     misc_feature    5251..5484
                     /label=SIN
                     /note="color: #cc0000; direction: RIGHT"
     LTR             5251..5484
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
                     /note="color: #ffe4c4"
     polyA_signal    5556..5677
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
                     /note="color: #a6acb3"
     rep_origin      5696..5831
                     /label=SV40 ori
                     /note="SV40 origin of replication"
                     /note="color: #ffff00"
     promoter        complement(5864..5882)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /note="color: #ffffff; direction: LEFT"
     primer_bind     complement(5892..5908)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: LEFT"
     rep_origin      6053..6508
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
     promoter        6537..6641
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: RIGHT"
     CDS             6642..7502
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 6642 .. 6710 / #ccffcc / signal sequence
                        2: 6711 .. 7502 / #ccffcc
                     Cleavage site after base 6710"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      7673..8261
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     protein_bind    8549..8570
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
                     /note="color: #31849b"
     promoter        8585..8615
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
                     /note="This forward directional feature has 3 segments:
                        1: 8585 .. 8590 / #ffffff / -35
                        2: 8591 .. 8608 / #ffffff
                        3: 8609 .. 8615 / #ffffff / -10"
     protein_bind    8623..8639
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #31849b"
     primer_bind     8647..8663
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: RIGHT"
     promoter        8684..8702
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
                     /note="color: #ffffff; direction: RIGHT"
ORIGIN
        1 agcttaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac gatgagttag
       61 caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg
      121 tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact
      181 gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacaa taaacgggtc
      241 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
      301 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
      361 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg
      421 cgcccgaaca gggacctgaa agcgaaaggg aaaccagagc tctctcgacg caggactcgg
      481 cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt
      541 ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg
      601 agaattagat cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa
      661 ttaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg
      721 ttagaaacat cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca
      781 ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa
      841 aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa
      901 agtaagacca ccgcacagca agcggccgct gatcttcaga cctggaggag gagatatgag
      961 ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt
     1021 agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg
     1081 agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag cctcaatgac
     1141 gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct
     1201 gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct
     1261 ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg
     1321 gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa
     1381 taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa
     1441 caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa
     1501 tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac
     1561 aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag
     1621 aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc
     1681 gtttcagacc cacctcccaa ccccgagggg acccgacagg cccgaaggaa tagaagaaga
     1741 aggtggagag agagacagag acagatccat tcgattagtg aacggatctc gacggtatcg
     1801 gttaactttt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga
     1861 cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa aaattcaaaa
     1921 ttttatcgat cacgagacta gcctcgagct agctgcagta acgccatttt gcaaggcatg
     1981 gaaaaatacc aaaccaagaa tagagaagtt cagatcaagg gcgggtacat gaaaatagct
     2041 aacgttgggc caaacaggat atctgcggtg agcagtttcg gccccggccc ggggccaaga
     2101 acagatggtc accgcagttt cggccccggc ccgaggccaa gaacagatgg tccccagata
     2161 tggcccaacc ctcagcagtt tcttaagacc catcagatgt ttccaggctc ccccaaggac
     2221 ctgaaatgac cctgcgcctt atttgaatta accaatcagc ctgcttctcg cttctgttcg
     2281 cgcgcttctg cttcccgagc tctataaaag agctcacaac ccctcactcg gcgcgccagt
     2341 cctccgacag actgagtcgg ccggtcgaat caagcttatc gataccgtcg acggatccac
     2401 cggtgccacc atgggatacc cctacgacgt gcccgactac cgtatccccg tagatgccag
     2461 cacgagccgc cgcttcacgc cgccttccac cgcgctgagc ccaggcaaga tgagcgaggc
     2521 gttgccgctg ggcgctcctg acgccggcgc tgccctggcc ggcaagctga ggagcggcga
     2581 ccgcagcatg gtggaggtgc tggccgacca cccgggcgag ctggtgcgca ccgacagccc
     2641 caacttcctc tgctccgtgc tgcctacgca ctggcgctgc aacaagaccc tgcccatcgc
     2701 tttcaaggtg gtggccctag gggatgttcc agatggcact ctggtcactg tgatggctgg
     2761 caatgatgaa aactactcgg ctgagctgag aaatgctacc gcagccatga agaaccaggt
     2821 tgcaagattt aatgacctca ggtttgtcgg tcgaagtgga agagggaaaa gcttcactct
     2881 gaccatcact gtcttcacaa acccaccgca agtcgccacc taccacagag ccatcaaaat
     2941 cacagtggat gggccccgag aacctcgaag acatcggcag aaactagatg atcagaccaa
     3001 gcccgggagc ttgagcttca gcgagagact gagtgaactg gagcagctgc ggcgcacagc
     3061 catgagggtc agcccacacc acccagcccc cacgcccaac cctcgtgcct ccctgaacca
     3121 ctccactgcc tttaaccctc agcctcagag tcagatgcag gaggaagaca cagcaccctg
     3181 gagatgttga acgcgtatgc atgagtaact gaggatccgc ccctctccct cccccccccc
     3241 taacgttact ggccgaagcc gcttggaata aggccggtgt gcgtttgtct atatgttatt
     3301 ttccaccata ttgccgtctt ttggcaatgt gagggcccgg aaacctggcc ctgtcttctt
     3361 gacgagcatt cctaggggtc tttcccctct cgccaaagga atgcaaggtc tgttgaatgt
     3421 cgtgaaggaa gcagttcctc tggaagcttc ttgaagacaa acaacgtctg tagcgaccct
     3481 ttgcaggcag cggaaccccc cacctggcga caggtgcctc tgcggccaaa agccacgtgt
     3541 ataagataca cctgcaaagg cggcacaacc ccagtgccac gttgtgagtt ggatagttgt
     3601 ggaaagagtc aaatggctct cctcaagcgt attcaacaag gggctgaagg atgcccagaa
     3661 ggtaccccat tgtatgggat ctgatctggg gcctcggtgc acatgcttta catgtgttta
     3721 gtcgaggtta aaaaaacgtc taggcccccc gaaccacggg gacgtggttt tcctttgaaa
     3781 aacacgatga taatatggcc acaaccatgg tgagcaaggg cgaggagctg ttcaccgggg
     3841 tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg
     3901 gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg
     3961 gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctacggc gtgcagtgct
     4021 tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag
     4081 gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg
     4141 aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca
     4201 aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct
     4261 atatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaaca
     4321 tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg
     4381 gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccgccctg agcaaagacc
     4441 ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc
     4501 tcggcatgga cgagctgtac aagtaaagcg gccgcgtcga cggatccccc gggctgcagg
     4561 aattcgagca tcttaccgcc atttataccc atatttgttc tgtttttctt gatttgggta
     4621 tacatttaaa tgttaataaa acaaaatggt ggggcaatca tttacatttt tagggatatg
     4681 taattactag ttcaggtgta ttgccacaag acaaacatgt taagaaactt tcccgttatt
     4741 tacgctctgt tcctgttaat caacctctgg attacaaaat ttgtgaaaga ttgactgata
     4801 ttcttaacta tgttgctcct tttacgctgt gtggatatgc tgctttaatg cctctgtatc
     4861 atgctattgc ttcccgtacg gctttcgttt tctcctcctt gtataaatcc tggttgctgt
     4921 ctctttatga ggagttgtgg cccgttgtcc gtcaacgtgg cgtggtgtgc tctgtgtttg
     4981 ctgacgcaac ccccactggc tggggcattg ccaccacctg tcaactcctt tctgggactt
     5041 tcgctttccc cctcccgatc gccacggcag aactcatcgc cgcctgcctt gcccgctgct
     5101 ggacaggggc taggttgctg ggcactgata attccgtggt gttgtcgggg aagctgacgt
     5161 cctttcgaat tcgatatcag gtacctttaa gaccaatgac ttacaaggca gctgtagatc
     5221 ttagccactt tttaaaagaa aaggggggac tggaagggct acgtaactcc caacgaagac
     5281 aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc
     5341 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
     5401 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
     5461 agtcagtgtg gaaaatctct agcagtagta gttcatgtca tcttattatt cagtatttat
     5521 aacttgcaaa gaaatgaata tcagagagtg agaggaactt gtttattgca gcttataatg
     5581 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
     5641 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggctc tagctatccc
     5701 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
     5761 ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt
     5821 ttttggaggc ctaggctttt gcgtcgagac gtacccaatt cgccctatag tgagtcgtat
     5881 tacgcgcgct cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc
     5941 caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc
     6001 cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggcg cgacgcgccc
     6061 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
     6121 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
     6181 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
     6241 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc
     6301 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
     6361 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt
     6421 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
     6481 tttaacaaaa tattaacgtt tacaatttcc caggtggcac ttttcgggga aatgtgcgcg
     6541 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
     6601 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
     6661 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
     6721 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
     6781 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
     6841 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag
     6901 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
     6961 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
     7021 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
     7081 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
     7141 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa
     7201 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
     7261 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
     7321 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
     7381 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
     7441 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
     7501 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
     7561 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
     7621 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     7681 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     7741 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     7801 cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact
     7861 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     7921 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     7981 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     8041 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     8101 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     8161 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     8221 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     8281 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
     8341 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
     8401 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac
     8461 cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact
     8521 ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc
     8581 aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat
     8641 ttcacacagg aaacagctat gaccatgatt acgccaagcg cgcaattaac cctcactaaa
     8701 gggaacaaaa gctggagctg ca
//
