LOCUS       Exported                9427 bp DNA     circular SYN 14-JAN-2022
DEFINITION  Ligation of RUNX1C* into Fragment 2.
ACCESSION   .
VERSION     .
KEYWORDS    DHV20+RUNX1C
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9427)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 14, 2022 from SnapGene Viewer 6.0.0
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9427
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    4..235
                     /label=RSV
                     /note="color: #cc0000; direction: RIGHT"
     misc_feature    236..417
                     /label=RU5
                     /note="color: #cc0000; direction: RIGHT"
     LTR             236..416
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /note="color: #ffe4c4"
     misc_feature    418..568
                     /label=PBS
                     /note="color: #cc0000; direction: RIGHT"
     misc_feature    460..585
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
                     /note="color: #ffe4c4"
     misc_feature    569..933
                     /label=dGag
                     /note="color: #cc0000; direction: RIGHT"
     misc_feature    1078..1311
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
                     /note="color: #ffe4c4"
     misc_feature    1079..1320
                     /label=RRE
                     /note="color: #cc0000; direction: RIGHT"
     CDS             1496..1540
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /note="color: #cc99b2"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1804..1927
                     /label=PPT
                     /note="color: #cc0000; direction: RIGHT"
     misc_feature    1807..1924
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
                     /note="color: #ffe4c4"
     misc_feature    1947..2347
                     /label=SFFV
                     /note="color: #cc0000; direction: RIGHT"
     promoter        1958..2364
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
                     /note="color: #ffffff; direction: RIGHT"
     misc_feature    2400..2415
                     /label='MCS''
                     /note="color: #cc0000; direction: RIGHT"
     regulatory      2421..2430
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /note="color: #a6acb3"
                     /regulatory_class="other"
     CDS             2427..3896
                     /codon_start=1
                     /label=RUNX1C
                     /note="color: #cc0000"
                     /translation="MGYPYDVPDYASDSIFESFPSYPQCFMRECILGMNPSRDVHDAST
                     SRRFTPPSTALSPGKMSEALPLGAPDAGAALAGKLRSGDRSMVEVLADHPGELVRTDSP
                     NFLCSVLPTHWRCNKTLPIAFKVVALGDVPDGTLVTVMAGNDENYSAELRNATAAMKNQ
                     VARFNDLRFVGRSGRGKSFTLTITVFTNPPQVATYHRAIKITVDGPREPRRHRQKLDDQ
                     TKPGSLSFSERLSELEQLRRTAMRVSPHHPAPTPNPRASLNHSTAFNPQPQSQMQDTRQ
                     IQPSPPWSYDQSYQYLGSIASPSVHPATPISPGRASGMTTLSAELSSRLSTAPDLTAFS
                     DPRQFPALPSISDPRMHYPGAFTYSPTPVTSGIGIGMSAMGSATRYHTYLPPPYPGSSQ
                     AQGGPFQASSPSYHLYYGASAGSYQFSMVGGERSPPRILPPCTNASTGSALLNPSLPNQ
                     SDVVEAEGSHSNSPTNMAPSARLEEAVWRPY"
     misc_feature    2430..2456
                     /label=HA
                     /note="color: #cc0000; direction: RIGHT"
     CDS             2433..2459
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="color: #cc99b2"
                     /translation="YPYDVPDYA"
     CDS             3113..3135
                     /codon_start=1
                     /label=SK_RUNX1.1
                     /note="as, mutated"
                     /note="color: #cc0000"
                     /translation="LQRETE"
     misc_feature    3280..3302
                     /label=SK_RUNX1.4
                     /note="as, mutated"
                     /note="color: #cc0000; direction: RIGHT"
     misc_feature    3282..3303
                     /label=SK_RUNX1.2
                     /note="as, mutated"
                     /note="color: #cc0000; direction: RIGHT"
     misc_feature    3897..3907
                     /label=''MCS
                     /note="color: #cc0000; direction: RIGHT"
     CDS             3907..3909
                     /codon_start=1
                     /label=STOP
                     /note="color: #cc0000"
                     /translation=""
     CDS             3911..3913
                     /codon_start=1
                     /label=STOP
                     /note="color: #cc0000"
                     /translation=""
     CDS             3915..3917
                     /codon_start=1
                     /label=STOP
                     /note="color: #cc0000"
                     /translation=""
     misc_feature    3924..4508
                     /label=IRES
                     /note="color: #cc0000; direction: RIGHT"
     misc_feature    3925..4511
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
                     /note="This feature has 3 segments:
                        1: 3925 .. 4499 / #a6acb3
                        2: 4500 .. 4502 / #a6acb3 / ATG
                        3: 4503 .. 4511 / #a6acb3"
     CDS             4500..5221
                     /codon_start=1
                     /label=EGFP'
                     /note="color: #cc0000"
                     /translation="MATTMVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYG
                     KLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
                     FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQK
                     NGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH
                     MVLLEFVTAAGITLGMDE"
     CDS             4512..5231
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /note="This feature has 3 segments:
                        1: 4512 .. 4514 / #05fd14
                        2: 4515 .. 4517 / #05fd14 / 1a
                        3: 4518 .. 5231 / #05fd14"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             5223..5231
                     /codon_start=1
                     /label='EGFP
                     /note="color: #cc0000"
                     /translation="YK"
     misc_feature    5270..5872
                     /label=PRE*
                     /note="color: #cc0000; direction: RIGHT"
     misc_feature    5463..5866
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
                     /note="color: #ffffff"
     misc_feature    5956..6189
                     /label=SIN
                     /note="color: #cc0000; direction: RIGHT"
     LTR             5956..6189
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
                     /note="color: #ffe4c4"
     polyA_signal    6261..6382
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
                     /note="color: #a6acb3"
     rep_origin      6401..6536
                     /label=SV40 ori
                     /note="SV40 origin of replication"
                     /note="color: #ffff00"
     promoter        complement(6569..6587)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /note="color: #ffffff; direction: LEFT"
     primer_bind     complement(6597..6613)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: LEFT"
     rep_origin      6758..7213
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
     promoter        7242..7346
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: RIGHT"
     CDS             7347..8207
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 7347 .. 7415 / #ccffcc / signal sequence
                        2: 7416 .. 8207 / #ccffcc
                     Cleavage site after base 7415"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      8378..8966
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     protein_bind    9254..9275
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
                     /note="color: #31849b"
     promoter        9290..9320
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
                     /note="This forward directional feature has 3 segments:
                        1: 9290 .. 9295 / #ffffff / -35
                        2: 9296 .. 9313 / #ffffff
                        3: 9314 .. 9320 / #ffffff / -10"
     protein_bind    9328..9344
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #31849b"
     primer_bind     9352..9368
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: RIGHT"
     promoter        9389..9407
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
                     /note="color: #ffffff; direction: RIGHT"
ORIGIN
        1 agcttaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac gatgagttag
       61 caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg
      121 tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact
      181 gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacaa taaacgggtc
      241 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
      301 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
      361 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg
      421 cgcccgaaca gggacctgaa agcgaaaggg aaaccagagc tctctcgacg caggactcgg
      481 cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt
      541 ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg
      601 agaattagat cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa
      661 ttaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg
      721 ttagaaacat cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca
      781 ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa
      841 aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa
      901 agtaagacca ccgcacagca agcggccgct gatcttcaga cctggaggag gagatatgag
      961 ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt
     1021 agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg
     1081 agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag cctcaatgac
     1141 gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct
     1201 gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct
     1261 ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg
     1321 gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa
     1381 taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa
     1441 caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa
     1501 tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac
     1561 aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag
     1621 aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc
     1681 gtttcagacc cacctcccaa ccccgagggg acccgacagg cccgaaggaa tagaagaaga
     1741 aggtggagag agagacagag acagatccat tcgattagtg aacggatctc gacggtatcg
     1801 gttaactttt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga
     1861 cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa aaattcaaaa
     1921 ttttatcgat cacgagacta gcctcgagct agctgcagta acgccatttt gcaaggcatg
     1981 gaaaaatacc aaaccaagaa tagagaagtt cagatcaagg gcgggtacat gaaaatagct
     2041 aacgttgggc caaacaggat atctgcggtg agcagtttcg gccccggccc ggggccaaga
     2101 acagatggtc accgcagttt cggccccggc ccgaggccaa gaacagatgg tccccagata
     2161 tggcccaacc ctcagcagtt tcttaagacc catcagatgt ttccaggctc ccccaaggac
     2221 ctgaaatgac cctgcgcctt atttgaatta accaatcagc ctgcttctcg cttctgttcg
     2281 cgcgcttctg cttcccgagc tctataaaag agctcacaac ccctcactcg gcgcgccagt
     2341 cctccgacag actgagtcgg ccggtcgaat caagcttatc gataccgtcg acggatccac
     2401 cggtactagt atactccgga gccaccatgg gataccccta cgacgtgccc gactacgctt
     2461 cagacagcat atttgagtca tttccttcgt acccacagtg cttcatgaga gaatgcatac
     2521 ttggaatgaa tccttctagg gacgtccacg atgccagcac gagccgccgc ttcacgccgc
     2581 cttccaccgc gctgagccca ggcaagatga gcgaggcgtt gccgctgggc gccccggacg
     2641 ccggcgctgc cctggccggc aagctgagga gcggcgaccg cagcatggtg gaggtgctgg
     2701 ccgaccaccc gggcgagctg gtgcgcaccg acagccccaa cttcctctgc tccgtgctgc
     2761 ctacgcactg gcgctgcaac aagaccctgc ccatcgcttt caaggtggtg gccctagggg
     2821 atgttccaga tggcactctg gtcactgtga tggctggcaa tgatgaaaac tactcggctg
     2881 agctgagaaa tgctaccgca gccatgaaga accaggttgc aagatttaat gacctcaggt
     2941 ttgtcggtcg aagtggaaga gggaaaagct tcactctgac catcactgtc ttcacaaacc
     3001 caccgcaagt cgccacctac cacagagcca tcaaaatcac agtggatggg ccccgagaac
     3061 ctcgaagaca tcggcagaaa ctagatgatc agaccaagcc cgggagcttg agcttcagcg
     3121 agagactgag tgaactggag cagctgcggc gcacagccat gagggtcagc ccacaccacc
     3181 cagcccccac gcccaaccct cgtgcctccc tgaaccactc cactgccttt aaccctcagc
     3241 ctcagagtca gatgcaggat acaaggcaga tccaaccatc tcctccctgg agctatgacc
     3301 agtcctacca atacctggga agcattgcct ctccttctgt gcacccagca acgcccattt
     3361 cacctggacg tgccagcggc atgacaaccc tctctgcaga actttccagt cgactctcaa
     3421 cggcacccga cctgacagcg ttcagcgacc cgcgccagtt ccccgcgctg ccctccatct
     3481 ccgacccccg catgcactat ccaggcgcct tcacctactc cccgacgccg gtcacctcgg
     3541 gcatcggcat cggcatgtcg gccatgggct cggccacgcg ctaccacacc tacctgccgc
     3601 cgccctaccc cggctcgtcg caagcgcagg gaggcccgtt ccaagccagc tcgccctcct
     3661 accacctgta ctacggcgcc tcggccggct cctaccagtt ctccatggtg ggcggcgagc
     3721 gctcgccgcc gcgcatcctg ccgccctgca ccaacgcctc caccggctcc gcgctgctca
     3781 accccagcct cccgaaccag agcgacgtgg tggaggccga gggcagccac agcaactccc
     3841 ccaccaacat ggcgccctcc gcgcgcctgg aggaggccgt gtggaggccc tactgacgcg
     3901 tatgcatgag taactgagga tccgcccctc tccctccccc ccccctaacg ttactggccg
     3961 aagccgcttg gaataaggcc ggtgtgcgtt tgtctatatg ttattttcca ccatattgcc
     4021 gtcttttggc aatgtgaggg cccggaaacc tggccctgtc ttcttgacga gcattcctag
     4081 gggtctttcc cctctcgcca aaggaatgca aggtctgttg aatgtcgtga aggaagcagt
     4141 tcctctggaa gcttcttgaa gacaaacaac gtctgtagcg accctttgca ggcagcggaa
     4201 ccccccacct ggcgacaggt gcctctgcgg ccaaaagcca cgtgtataag atacacctgc
     4261 aaaggcggca caaccccagt gccacgttgt gagttggata gttgtggaaa gagtcaaatg
     4321 gctctcctca agcgtattca acaaggggct gaaggatgcc cagaaggtac cccattgtat
     4381 gggatctgat ctggggcctc ggtgcacatg ctttacatgt gtttagtcga ggttaaaaaa
     4441 acgtctaggc cccccgaacc acggggacgt ggttttcctt tgaaaaacac gatgataata
     4501 tggccacaac catggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg
     4561 tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg
     4621 atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc
     4681 cctggcccac cctcgtgacc accctgacct acggcgtgca gtgcttcagc cgctaccccg
     4741 accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc
     4801 gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg
     4861 gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca
     4921 tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc atggccgaca
     4981 agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag gacggcagcg
     5041 tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc
     5101 ccgacaacca ctacctgagc acccagtccg ccctgagcaa agaccccaac gagaagcgcg
     5161 atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc
     5221 tgtacaagta aagcggccgc gtcgacggat cccccgggct gcaggaattc gagcatctta
     5281 ccgccattta tacccatatt tgttctgttt ttcttgattt gggtatacat ttaaatgtta
     5341 ataaaacaaa atggtggggc aatcatttac atttttaggg atatgtaatt actagttcag
     5401 gtgtattgcc acaagacaaa catgttaaga aactttcccg ttatttacgc tctgttcctg
     5461 ttaatcaacc tctggattac aaaatttgtg aaagattgac tgatattctt aactatgttg
     5521 ctccttttac gctgtgtgga tatgctgctt taatgcctct gtatcatgct attgcttccc
     5581 gtacggcttt cgttttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt
     5641 tgtggcccgt tgtccgtcaa cgtggcgtgg tgtgctctgt gtttgctgac gcaaccccca
     5701 ctggctgggg cattgccacc acctgtcaac tcctttctgg gactttcgct ttccccctcc
     5761 cgatcgccac ggcagaactc atcgccgcct gccttgcccg ctgctggaca ggggctaggt
     5821 tgctgggcac tgataattcc gtggtgttgt cggggaagct gacgtccttt cgaattcgat
     5881 atcaggtacc tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa
     5941 aagaaaaggg gggactggaa gggctacgta actcccaacg aagacaagat ctgctttttg
     6001 cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
     6061 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
     6121 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
     6181 tctctagcag tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat
     6241 gaatatcaga gagtgagagg aacttgttta ttgcagctta taatggttac aaataaagca
     6301 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     6361 ccaaactcat caatgtatct tatcatgtct ggctctagct atcccgcccc taactccgcc
     6421 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
     6481 ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
     6541 cttttgcgtc gagacgtacc caattcgccc tatagtgagt cgtattacgc gcgctcactg
     6601 gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt
     6661 gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct
     6721 tcccaacagt tgcgcagcct gaatggcgaa tggcgcgacg cgccctgtag cggcgcatta
     6781 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
     6841 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     6901 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     6961 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
     7021 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
     7081 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc
     7141 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta
     7201 acgtttacaa tttcccaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
     7261 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     7321 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
     7381 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
     7441 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
     7501 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
     7561 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
     7621 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
     7681 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     7741 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
     7801 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     7861 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
     7921 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
     7981 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
     8041 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
     8101 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     8161 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     8221 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     8281 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     8341 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     8401 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     8461 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     8521 ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     8581 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     8641 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     8701 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     8761 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     8821 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     8881 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     8941 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     9001 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     9061 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     9121 gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc
     9181 gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg
     9241 agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta
     9301 tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca
     9361 gctatgacca tgattacgcc aagcgcgcaa ttaaccctca ctaaagggaa caaaagctgg
     9421 agctgca
//
