LOCUS       Exported                8824 bp DNA     circular SYN 12-OCT-2021
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8824)
  AUTHORS   Sohum Mehta
  TITLE     Direct Submission
  JOURNAL   Exported Jan 14, 2022 from SnapGene 6.0.0
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8824
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        11..390
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
                     /note="color: #ffffff"
     promoter        391..594
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
                     /note="color: #ffffff; direction: RIGHT"
     promoter        639..657
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /note="color: #ffffff; direction: RIGHT"
     regulatory      665..674
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /note="color: #a6acb3"
                     /regulatory_class="other"
     CDS             671..1378
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /note="color: #ff0000"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     CDS             1379..4117
                     /codon_start=1
                     /label=human p115-RhoGEF
                     /note="color: #919191"
                     /translation="MEDFARGAASPGPSRPGLVPVSIIGAEDEDFENELETNSEEQNSQ
                     FQSLEQVKRRPAHLMALLQHVALQFEPGPLLCCLHADMLGSLGPKEAKKAFLDFYHSFL
                     EKTAVLRVPVPPNVAFELDRTRADLISEDVQRRFVQEVVQSQQVAVGRQLEDFRSKRLM
                     GMTPWEQELAQLEAWVGRDRASYEARERHVAERLLMHLEEMQHTISTDEEKSAAVVNAI
                     GLYMRHLGVRTKSGDKKSGRNFFRKKVMGNRRSDEPAKTKKGLSSILDAARWNRGEPQV
                     PDFRHLKAEVDAEKPGATDRKGGVGMPSRDRNIGAPGQDTPGVSLHPLSLDSPDREPGA
                     DAPLELGDSSPQGPMSLESLAPPESTDEGAETESPEPGDEGEPGRSGLELEPEEPPGWR
                     ELVPPDTLHSLPKSQVKRQEVISELLVTEAAHVRMLRVLHDLFFQPMAECLFFPLEELQ
                     NIFPSLDELIEVHSLFLDRLMKRRQESGYLIEEIGDVLLARFDGAEGSWFQKISSRFCS
                     RQSFALEQLKAKQRKDPRFCAFVQEAESRPRCRRLQLKDMIPTEMQRLTKYPLLLQSIG
                     QNTEEPTEREKVELAAECCREILHHVNQAVRDMEDLLRLKDYQRRLDLSHLRQSSDPML
                     SEFKNLDITKKKLVHEGPLTWRVTKDKAVEVHVLLLDDLLLLLQRQDERLLLKSHSRTL
                     TPTPDGKTMLRPVLRLTSAMTREVATDHKAFYVLFTWDQEAQIYELVAQTVSERKNWCA
                     LITETAGSLKVPAPASRPKPRPSPSSTREPLLSSSENGNGGRETSPADARTERILSDLL
                     PFCRPGPEGQLAATALRKVLSLKQLLFPAEEDNGAGPPRDGDGVPGGGPLSPARTQEIQ
                     ENLLSLEETMKQLEELEEEFCRLRPLLSQLGGNSVPQPGCT"
     polyA_signal    4200..4424
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
                     /note="color: #a6acb3"
     rep_origin      4470..4898
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
     promoter        4912..5241
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
                     /note="color: #ffffff; direction: RIGHT"
     rep_origin      5092..5227
                     /label=SV40 ori
                     /note="SV40 origin of replication"
                     /note="color: #ffff00"
     CDS             5308..6102
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /note="color: #ccffcc"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    6276..6397
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
                     /note="color: #a6acb3"
     primer_bind     complement(6446..6462)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: LEFT"
     protein_bind    6470..6486
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #31849b"
     promoter        complement(6494..6524)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
                     /note="This reverse directional feature has 3 segments:
                        1: 6494 .. 6500 / #ffffff / -10
                        2: 6501 .. 6518 / #ffffff
                        3: 6519 .. 6524 / #ffffff / -35"
     protein_bind    6539..6560
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
                     /note="color: #31849b"
     rep_origin      complement(6848..7433)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     CDS             complement(7604..8464)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 7604 .. 8395 / #ccffcc
                        2: 8396 .. 8464 / #ccffcc / signal sequence
                     Cleavage site after base 8395"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(8465..8569)
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: LEFT"
ORIGIN
        1 tatacgcgtt gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta
       61 gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc
      121 tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg
      181 ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg
      241 gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa
      301 tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac
      361 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
      421 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
      481 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
      541 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg
      601 ctaactagag aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag
      661 acccgccacc atggtgagca agggcgagga ggataacatg gccatcatca aggagttcat
      721 gcgcttcaag gtgcacatgg agggctccgt gaacggccac gagttcgaga tcgagggcga
      781 gggcgagggc cgcccctacg agggcaccca gaccgccaag ctgaaggtga ccaagggtgg
      841 ccccctgccc ttcgcctggg acatcctgtc ccctcagttc atgtacggct ccaaggccta
      901 cgtgaagcac cccgccgaca tccccgacta cttgaagctg tccttccccg agggcttcaa
      961 gtgggagcgc gtgatgaact tcgaggacgg cggcgtggtg accgtgaccc aggactcctc
     1021 cctgcaggac ggcgagttca tctacaaggt gaagctgcgc ggcaccaact tcccctccga
     1081 cggccccgta atgcagaaga agaccatggg ctgggaggcc tcctccgagc ggatgtaccc
     1141 cgaggacggc gccctgaagg gcgagatcaa gcagaggctg aagctgaagg acggcggcca
     1201 ctacgacgct gaggtcaaga ccacctacaa ggccaagaag cccgtgcagc tgcccggcgc
     1261 ctacaacgtc aacatcaagt tggacatcac ctcccacaac gaggactaca ccatcgtgga
     1321 acagtacgaa cgcgccgagg gccgccactc caccggcggc atggacgagc tgtacaagat
     1381 ggaagacttc gcccgagggg cggcctcccc aggcccctcc cggcctggcc tggttcccgt
     1441 cagcatcatc ggggctgagg atgaggattt tgagaacgag ctggagacaa actcagaaga
     1501 gcaaaacagc cagttccaga gcctggagca ggtgaagcgg cgcccagccc acctcatggc
     1561 cctcctgcag cacgtggccc tgcagtttga gccaggaccc ctgctttgct gtctgcatgc
     1621 cgacatgctg ggctcactgg gccccaagga ggccaagaag gccttcctgg acttctacca
     1681 cagcttcctg gagaagacag cggttctccg ggtgccggtc cctcccaacg tcgcctttga
     1741 acttgaccgc actagggctg acctcatctc cgaggatgtc cagcggcggt tcgtgcagga
     1801 ggtggtgcaa agccagcagg tagccgtggg ccggcagctg gaggacttcc gttccaagcg
     1861 gctcatgggc atgacgccct gggagcagga gctggcccag ctggaggctt gggttgggcg
     1921 ggaccgagcc agctacgagg cccgggagcg gcacgtggcg gagcggctgc tcatgcacct
     1981 ggaggagatg caacatacca tctctaccga cgaagaaaag agtgctgccg tggtcaacgc
     2041 cattggcctg tacatgcgcc accttggggt gcggaccaag agtggagaca agaagtcggg
     2101 gaggaacttc ttccggaaaa aggtgatggg gaaccggcgg tcggacgagc ctgccaagac
     2161 caagaagggg ctgagcagca tcctggatgc cgcccgctgg aaccggggag agccccaggt
     2221 tccagatttt cgacacctca aagcagaggt tgatgccgag aagccaggtg ctacagaccg
     2281 gaagggaggc gtggggatgc cctctcggga ccggaatatc ggggctcctg ggcaggacac
     2341 ccctggagtc tctctgcacc ctctgtccct ggacagccca gaccgggaac caggtgctga
     2401 cgcccccctg gagctggggg actcatcccc gcagggccca atgagcctgg agtccttggc
     2461 gcccccagag agtaccgacg agggggccga aaccgagagc cccgagcctg gagatgaggg
     2521 ggagccgggg cggtcgggac tggagcttga accagaagag cctcccggct ggcgggaact
     2581 cgtcccccca gacaccctgc acagcctgcc caagagccag gtgaagcggc aggaggtcat
     2641 cagcgagctg ctggtgacag aggcggccca cgtgcgcatg ctgcgggtgc tgcacgacct
     2701 cttcttccag cccatggcag aatgcctgtt cttccccttg gaggagctgc agaacatctt
     2761 ccccagcctg gacgagctca tcgaggtgca ttccctgttc ctcgatcgcc tgatgaagcg
     2821 gaggcaggag agtggctacc tcatcgagga gatcggagac gtgctgctgg cccggtttga
     2881 tggtgctgag ggctcctggt tccagaaaat ctcctcccgc ttctgcagcc gccagtcatt
     2941 tgccttagag cagctcaaag ccaagcaacg caaggaccct cggttctgtg ccttcgtgca
     3001 ggaagctgag agccgcccgc ggtgccgccg cctgcagctg aaggacatga tccccacgga
     3061 gatgcagcgg ctgaccaagt accccctgct cctgcagagc atcgggcaga acacagaaga
     3121 gcccacagaa cgggagaaag tggagctggc agccgagtgc tgccgggaaa ttctacacca
     3181 cgtcaaccaa gccgtgcgtg acatggagga cctgctgagg ctcaaggact atcagcggcg
     3241 cctggacttg tcccaccttc ggcagagcag cgaccctatg ctgagcgagt tcaagaacct
     3301 ggacatcacc aagaagaaat tggtccacga gggcccactg acgtggcggg tgactaagga
     3361 caaggcagtg gaggtgcatg tgctgctgct ggacgacctg ctgctgctgc tccagcgcca
     3421 ggacgagcgg ctgctgctca agtcccatag ccggacactg acgcccacgc ccgatggcaa
     3481 gaccatgctg cggcccgtgc tgcggctcac ctccgccatg acccgcgagg tggccaccga
     3541 tcacaaagcc ttctacgtcc tttttacctg ggaccaggag gcccagatat acgagctggt
     3601 ggcacagact gtgtcggagc ggaaaaactg gtgtgctctc atcactgaga ctgccggatc
     3661 cctgaaagtc cctgcccctg cctctcgccc taagccccgg cccagcccga gcagcacccg
     3721 agaacccctc ctcagcagct ctgagaacgg caatggtggc cgagagacgt ctccagctga
     3781 tgcccggacc gagagaatcc tcagtgacct cctgcccttc tgcagaccag gccccgaggg
     3841 ccagctcgct gccacggccc ttcggaaagt gctgtccctg aagcagcttc tgtttccggc
     3901 ggaggaagac aatggggcgg ggcctcctcg agatggggat ggggtcccag ggggcggccc
     3961 cctgagccca gcacggaccc aggaaatcca ggagaacctg ctcagcttgg aggagaccat
     4021 gaagcagctg gaggagttgg aggaggaatt ttgccgcctg agacccctcc tgtctcagct
     4081 tggggggaac tctgtccccc agcctggctg cacttgagaa ttctgcagat atccagcaca
     4141 gtggcggccg ctcgagcatg catctagagg gcccgtttaa acccgctgat cagcctcgac
     4201 tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct
     4261 ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct
     4321 gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg
     4381 ggaagacaat agcaggcatg ctggggatgc ggtgggctct atggcttctg aggcggaaag
     4441 aaccagctgg ggctctaggg ggtatcccca cgcgccctgt agcggcgcat taagcgcggc
     4501 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc
     4561 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa
     4621 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact
     4681 tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt
     4741 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa
     4801 ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt
     4861 aaaaaatgag ctgatttaac aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag
     4921 ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
     4981 aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
     5041 agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc
     5101 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
     5161 gcagaggccg aggccgcctc tgcctctgag ctattccaga agtagtgagg aggctttttt
     5221 ggaggcctag gcttttgcaa aaagctcccg ggagcttgta tatccatttt cggatctgat
     5281 caagagacag gatgaggatc gtttcgcatg attgaacaag atggattgca cgcaggttct
     5341 ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc
     5401 tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc
     5461 gacctgtccg gtgccctgaa tgaactgcag gacgaggcag cgcggctatc gtggctggcc
     5521 acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg
     5581 ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag
     5641 aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc
     5701 ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt
     5761 cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc
     5821 gccaggctca aggcgcgcat gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc
     5881 tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg
     5941 ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag
     6001 cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg
     6061 cagcgcatcg ccttctatcg ccttcttgac gagttcttct gagcgggact ctggggttcg
     6121 aaatgaccga ccaagcgacg cccaacctgc catcacgaga tttcgattcc accgccgcct
     6181 tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc
     6241 gcggggatct catgctggag ttcttcgccc accccaactt gtttattgca gcttataatg
     6301 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
     6361 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctgtata ccgtcgacct
     6421 ctagctagag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
     6481 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
     6541 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
     6601 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
     6661 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
     6721 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     6781 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     6841 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     6901 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     6961 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     7021 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     7081 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     7141 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     7201 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     7261 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
     7321 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     7381 gcggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     7441 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     7501 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     7561 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     7621 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     7681 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     7741 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     7801 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     7861 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
     7921 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     7981 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     8041 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     8101 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     8161 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     8221 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     8281 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     8341 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     8401 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     8461 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
     8521 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
     8581 gaaaagtgcc acctgacgtc gacggatcgg gagatctccc gatcccctat ggtgcactct
     8641 cagtacaatc tgctctgatg ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt
     8701 ggaggtcgct gagtagtgcg cgagcaaaat ttaagctaca acaaggcaag gcttgaccga
     8761 caattgcatg aagaatctgc ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc
     8821 caga
//
