LOCUS       YY29_pAAV-CAG(short)-Hc 6735 bp ds-DNA     circular     22-SEP-2021
DEFINITION  .
KEYWORDS    "creator:Baylor-NRI" "marker:AmpR, R-ITR, L-ITR"                   
FEATURES             Location/Qualifiers
     misc_feature    1..141
                     /label="L-ITR"
                     /ApEinfo_revcolor=#3fbf3f
                     /ApEinfo_fwdcolor=#3fbf3f
     misc_feature    151..1277
                     /label="KC103F"
                     /ApEinfo_revcolor=#3f3fbf
                     /ApEinfo_fwdcolor=#3f3fbf
     promoter        164..1263
                     /label="CAG"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     primer          1279..1321
                     /label="5 Hypho to pCAG"
                     /note="sequence: gcgggcccgggatccggcgcgccATGCCTTTCTACGACAGCCG"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    1288..1293
                     /label="BamHI"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    1294..1301
                     /label="AscI"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    1302..2096
                     /label="KCR1"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     CDS             1302..2819
                     /label="Translation 1302-2819"
     primer          complement(2076..2117)
                     /label="3 Hypho to EYFP"
                     /note="sequence: CTCGCCCTTGCTCACgttaacGGACAGTGTCTCAGGCTCATT"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    2097..2102
                     /label="HpaI"
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_fwdcolor=#ff0000
     primer          2097..2119
                     /label="YY8"
                     /note="sequence: gttaacGTGAGCAAGGGCGAGGA"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     exon            2103..2816
                     /label="EYFP (no start)"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     primer          2227..2250
                     /label="EYFP F128 Xue10"
                     /note="sequence: TGACCCTGAAGTTCATCTGCACCA"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     primer          complement(2346..2365)
                     /label="EYFP R266 Xue 12"
                     /note="sequence: ATGGCGGACTTGAAGAAGTC"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     primer          complement(2442..2461)
                     /label="EYFP-R399"
                     /note="sequence: ACCAGGGTGTCGCCCTCGAA"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     primer          2538..2558
                     /label="EYFP/EGFP F439 Xue20"
                     /note="sequence: AACAGCCACAACGTCTATATC"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     primer          2587..2610
                     /label="EYFP F488 Xue11"
                     /note="sequence: AGGTGAACTTCAAGATCCGCCACA"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     primer          complement(2797..2840)
                     /label="3 EYFP to pCAG"
                     /note="sequence: tttaagcttgaattcgctagctcaCTTGTACAGCTCGTCCATGC"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    2820..2825
                     /label="NheI"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    2826..2826
                     /label="EcoRI"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    2827..2831
                     /label="EcoRI"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    2832..2837
                     /label="HindIII"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    2852..3460
                     /label="WPRE"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     polyA_signal    3480..3958
                     /label="hGH polyA"
                     /ApEinfo_revcolor=#8000ff
                     /ApEinfo_fwdcolor=#8000ff
     misc_feature    3998..4138
                     /label="R-ITR"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     rep_origin      4230..4536
                     /label="f1 Origin"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    5055..5912
                     /label="AmpR"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     rep_origin      6063..6730
                     /label="pUC ori"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtaacac tagttattaa tagtaatcaa
      181 ttacggggtc attagttcat agcccatata tggagttccg cgttacataa cttacggtaa
      241 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
      301 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
      361 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
      421 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
      481 ctacttggca gtacatctac gtattagtca tcgctattac catggtcgag gtgagcccca
      541 cgttctgctt cactctcccc atctcccccc cctccccacc cccaattttg tatttattta
      601 ttttttaatt attttgtgca gcgatggggg cggggggggg gggggggcgc gcgccaggcg
      661 gggcggggcg gggcgagggg cggggcgggg cgaggcggag aggtgcggcg gcagccaatc
      721 agagcggcgc gctccgaaag tttcctttta tggcgaggcg gcggcggcgg cggccctata
      781 aaaagcgaag cgcgcggcgg gcgggagtcg ctgcgcgctg ccttcgcccc gtgccccgct
      841 ccgccgccgc ctcgcgccgc ccgccccggc tctgactgac cgcgttactc ccacaggtga
      901 gcgggcggga cggcccttct cctccgggct gtaattagcg cttggtttaa tgacggcttg
      961 tttcttttct gtggctgcgt gaaagccttg aggggctccg ggagcgccgg caggaaggaa
     1021 atgggcgggg agggccttcg tgcgtcgccg cgccgccgtc cccttctccc tctccagcct
     1081 cggggctgtc cgcgggggga cggctgcctt cgggggggac ggggcagggc ggggttcggc
     1141 ttctggcgtg tgaccggcgg ctctagagcc tctgctaacc atgttcatgc cttcttcttt
     1201 ttcctacagc tcctgggcaa cgtgctggtt attgtgctgt ctcatcattt tggcaaagaa
     1261 ttgcggccca acggtaccgc gggcccggga tccggcgcgc cATGCCTTTC TACGACAGCC
     1321 GGCCCCCTGA GGGATGGCCT AAGGGCTCCA TCAACGACAT GGATTATCCA CTGCTGGGCT
     1381 CTATTTGTGC CGTGTGCTGC GTGTTCGTGG CAGGCAGCGG CATCTGGATG CTGTACAGGC
     1441 TGGATCTGGG CATGGGCTAC TCTTGTAAGC CATATAAAAG CGGCCGGGCA CCTGAGGTGA
     1501 ACAGCCTGTC CGGCATCATC TGCCTGCTGT GCGGCACAAT GTACGCCGCC AAGTCCTTCG
     1561 ACTTCTTTGA TGGCGGCGGA ACCCCCTTTT CTCTGAATTG GTACTGGTAT CTGGACTACG
     1621 TGTTCACCTG CCCCCTGCTG ATCCTGGACT TCGCCTTTAC CCTGGATCTG CCTCACAAGA
     1681 TCCGGTACTT CTTTGCCGTG TTTCTGACCC TGTGGTGTGG AGTGGCAGCC TTCGTGACAC
     1741 CAAGCGCCTA TAGATTTGCC TACTATGCCC TGGGCTGCTG TTGGTTCACA CCCTTTGCCC
     1801 TGTCCCTGAT GCGGCACGTG AAGGAGAGAT ACCTGGTGTA TCCACCCAAG TGCCAGAGGT
     1861 GGCTGTTCTG GGCCTGCGTG ATCTTCTTTG GCTTCTGGCC TATGTTTCCA ATCCTGTTCA
     1921 TCTTTTCCTG GCTGGGCACC GGCCACATCT CTCAGCAGGC CTTCTACATC ATCCACGCCT
     1981 TTCTGGATCT GACCTGCAAG TCTATCTTCG GCATCCTGAT GACAGTGTTT CGCCTGGAGC
     2041 TGGAGGAGCA CACAGAGGTG CAGGGACTGC CCCTGAATGA GCCTGAGACA CTGTCCgtta
     2101 acGTGAGCAA GGGCGAGGAG CTGTTCACCG GGGTGGTGCC CATCCTGGTC GAGCTGGACG
     2161 GCGACGTAAA CGGCCACAAG TTCAGCGTGT CCGGCGAGGG CGAGGGCGAT GCCACCTACG
     2221 GCAAGCTGAC CCTGAAGTTC ATCTGCACCA CCGGCAAGCT GCCCGTGCCC TGGCCCACCC
     2281 TCGTGACCAC CTTCGGCTAC GGCCTGCAGT GCTTCGCCCG CTACCCCGAC CACATGAAGC
     2341 AGCACGACTT CTTCAAGTCC GCCATGCCCG AAGGCTACGT CCAGGAGCGC ACCATCTTCT
     2401 TCAAGGACGA CGGCAACTAC AAGACCCGCG CCGAGGTGAA GTTCGAGGGC GACACCCTGG
     2461 TGAACCGCAT CGAGCTGAAG GGCATCGACT TCAAGGAGGA CGGCAACATC CTGGGGCACA
     2521 AGCTGGAGTA CAACTACAAC AGCCACAACG TCTATATCAT GGCCGACAAG CAGAAGAACG
     2581 GCATCAAGGT GAACTTCAAG ATCCGCCACA ACATCGAGGA CGGCAGCGTG CAGCTCGCCG
     2641 ACCACTACCA GCAGAACACC CCCATCGGCG ACGGCCCCGT GCTGCTGCCC GACAACCACT
     2701 ACCTGAGCTA CCAGTCCGCC CTGAGCAAAG ACCCCAACGA GAAGCGCGAT CACATGGTCC
     2761 TGCTGGAGTT CGTGACCGCC GCCGGGATCA CTCTCGGCAT GGACGAGCTG TACAAGtgag
     2821 ctagcgaatt caagcttaaa ttcgatatca agcttatcga taatcaacct ctggattaca
     2881 aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat
     2941 acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct
     3001 ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac
     3061 gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca
     3121 cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca
     3181 tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg
     3241 tggtgttgtc ggggaaatca tcgtcctttc cttggctgct cgcctatgtt gccacctgga
     3301 ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt
     3361 cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga
     3421 gtcggatctc cctttgggcc gcctccccgc atcgataccg agcgctgctc gagagatcta
     3481 cgggtggcat ccctgtgacc cctccccagt gcctctcctg gccctggaag ttgccactcc
     3541 agtgcccacc agccttgtcc taataaaatt aagttgcatc attttgtctg actaggtgtc
     3601 cttctataat attatggggt ggaggggggt ggtatggagc aaggggcaag ttgggaagac
     3661 aacctgtagg gcctgcgggg tctattggga accaagctgg agtgcagtgg cacaatcttg
     3721 gctcactgca atctccgcct cctgggttca agcgattctc ctgcctcagc ctcccgagtt
     3781 gttgggattc caggcatgca tgaccaggct cagctaattt ttgttttttt ggtagagacg
     3841 gggtttcacc atattggcca ggctggtctc caactcctaa tctcaggtga tctacccacc
     3901 ttggcctccc aaattgctgg gattacaggc gtgaaccact gctcccttcc ctgtccttct
     3961 gattttgtag gtaaccacgt gcggaccgag cggccgcagg aacccctagt gatggagttg
     4021 gccactccct ctctgcgcgc tcgctcgctc actgaggccg ggcgaccaaa ggtcgcccga
     4081 cgcccgggct ttgcccgggc ggcctcagtg agcgagcgag cgcgcagctg cctgcagggg
     4141 cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catacgtcaa
     4201 agcaaccata gtacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc
     4261 gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt
     4321 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag
     4381 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgatttg ggtgatggtt
     4441 cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt
     4501 tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc tcgggctatt
     4561 cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt
     4621 aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt tacaatttta tggtgcactc
     4681 tcagtacaat ctgctctgat gccgcatagt taagccagcc ccgacacccg ccaacacccg
     4741 ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg
     4801 tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgagacgaa
     4861 agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga
     4921 cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
     4981 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     5041 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
     5101 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
     5161 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
     5221 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
     5281 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt
     5341 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
     5401 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
     5461 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
     5521 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
     5581 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
     5641 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
     5701 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
     5761 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
     5821 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
     5881 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
     5941 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     6001 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     6061 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     6121 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     6181 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag
     6241 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     6301 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     6361 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     6421 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     6481 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     6541 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     6601 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     6661 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     6721 cttttgctca catgt
//