LOCUS       Exported                8868 bp DNA     circular SYN 12-OCT-2021
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8868)
  AUTHORS   Sohum Mehta
  TITLE     Direct Submission
  JOURNAL   Exported Jan 18, 2022 from SnapGene 6.0.0
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8868
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
                     /note="color: #ffffff"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
                     /note="color: #ffffff; direction: RIGHT"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /note="color: #ffffff; direction: RIGHT"
     CDS             919..1617
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP(I174A)
                     /note="mammalian codon-optimized"
                     /note="color: #3366ff"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     CDS             1636..4425
                     /codon_start=1
                     /label=human MRTF-A
                     /note="color: #011993"
                     /translation="MPPLKSPAAFHEQRRSLERARTEDYLKRKIRSRPERSELVRMHIL
                     EETSAEPSLQAKQLKLKRARLADDLNEKIAQRPGPMELVEKNILPVESSLKEAIIVGQV
                     NYPKVADSSSFDEDSSDALSPEQPASHESQGSVPSPLEARVSEPLLSATSASPTQVVSQ
                     LPMGRDSREMLFLAEQPPLPPPPLLPPSLTNGTTIPTAKSTPTLIKQSQPKSASEKSQR
                     SKKAKELKPKVKKLKYHQYIPPDQKQDRGAPPMDSSYAKILQQQQLFLQLQILNQQQQQ
                     HHNYQAILPAPPKSAGEALGSSGTPPVRSLSTTNSSSSSGAPGPCGLARQNSTSLTGKP
                     GALPANLDDMKVAELKQELKLRSLPVSGTKTELIERLRAYQDQISPVPGAPKAPAATSI
                     LHKAGEVVVAFPAARLSTGPALVAAGLAPAEVVVATVASSGVVKFGSTGSTPPVSPTPS
                     ERSLLSTGDENSTPGDTFGEMVTSPLTQLTLQASPLQILVKEEGPRAGSCCLSPGGRAE
                     LEGRDKDQMLQEKDKQIEALTRMLRQKQQLVERLKLQLEQEKRAQQPAPAPAPLGTPVK
                     QESSFSSCQLSQQPLGPAHPFNPSLAAPATNHIDPCAVAPGPPSVVVKQEALQPEPEPV
                     PAPQLLLGPQGPSLIKGVAPPTLITDSTGTHLVLTVTNKNADSPGLSSGSPQQPSSQPG
                     SPAPAPSAQMDLEHPLQPLFGAPTSLLKKEPPGYEEAMSQQPKQQENGSSSQQMDDLFD
                     ILIQSGEISADFKEPPSLPGKEKPSPKTVCGSPLAAQPSPSAELPQAAPPPPGSPSLPG
                     RLEDFLESSTGLPLLTSGHDGPEPLSLIDDLHSQMLSSTAILDHPPSPMDTSELHFVPE
                     PSSTMGLDLADGHLDSMDWLELSSGGPVLSLAPLSTTAPSLFSTDFLDGHDLQLHWDSC
                     "
     polyA_signal    4469..4693
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
                     /note="color: #a6acb3"
     rep_origin      4739..5167
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
     promoter        5181..5510
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
                     /note="color: #ffffff; direction: RIGHT"
     rep_origin      5361..5496
                     /label=SV40 ori
                     /note="SV40 origin of replication"
                     /note="color: #ffff00"
     CDS             5577..6371
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /note="color: #ccffcc"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    6545..6666
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
                     /note="color: #a6acb3"
     primer_bind     complement(6715..6731)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: LEFT"
     protein_bind    6739..6755
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #31849b"
     promoter        complement(6763..6793)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
                     /note="This reverse directional feature has 3 segments:
                        1: 6763 .. 6769 / #ffffff / -10
                        2: 6770 .. 6787 / #ffffff
                        3: 6788 .. 6793 / #ffffff / -35"
     protein_bind    6808..6829
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
                     /note="color: #31849b"
     rep_origin      complement(7117..7702)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     CDS             complement(7873..8733)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 7873 .. 8664 / #ccffcc
                        2: 8665 .. 8733 / #ccffcc / signal sequence
                     Cleavage site after base 8664"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(8734..8838)
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: LEFT"
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaactt aagcttggat gagcgagctg attaaggaga acatgcacat gaagctgtac
      961 atggagggca ccgtggacaa ccatcacttc aagtgcacat ccgagggcga aggcaagccc
     1021 tacgagggca cccagaccat gagaatcaag gtggtcgagg gcggccctct ccccttcgcc
     1081 ttcgacatcc tggctactag cttcctctac ggcagcaaga ccttcatcaa ccacacccag
     1141 ggcatccccg acttcttcaa gcagtccttc cctgagggct tcacatggga gagagtcacc
     1201 acatacgaag acgggggcgt gctgaccgct acccaggaca ccagcctcca ggacggctgc
     1261 ctcatctaca acgtcaagat cagaggggtg aacttcacat ccaacggccc tgtgatgcag
     1321 aagaaaacac tcggctggga ggccttcacc gagacgctgt accccgctga cggcggcctg
     1381 gaaggcagaa acgacatggc cctgaagctc gtgggcggga gccatctgat cgcaaacgcc
     1441 aagaccacat atagatccaa gaaacccgct aagaacctca agatgcctgg cgtctactat
     1501 gtggactaca gactggaaag aatcaaggag gccaacaacg agacctacgt cgagcagcac
     1561 gaggtggcag tggccagata ctgcgacctc cctagcaaac tggggcacaa gcttaatgga
     1621 ggtactggtg gaagtatgcc gcctttgaaa agtccagccg catttcatga gcagagaagg
     1681 agcttggagc gggccaggac agaggactat ctcaaacgga agattcgttc ccggccggag
     1741 agatcggagc tggtcaggat gcacattttg gaagagacct cggctgagcc atccctccag
     1801 gccaagcagc tgaagctgaa gagagccaga ctagccgatg acctcaatga gaagattgca
     1861 cagaggcctg gccccatgga gctggtggag aagaacatcc ttcctgttga gtccagcctg
     1921 aaggaagcca tcattgtggg ccaggtgaac tatcccaaag tagcagacag ctcttccttc
     1981 gatgaggaca gcagcgatgc cttatccccc gagcagcctg ccagccatga gtcccagggt
     2041 tctgtgccgt cacccctgga ggcccgagtc agcgaaccac tgctcagtgc cacctctgca
     2101 tcccccaccc aggttgtgtc tcaacttccg atgggccggg attccagaga aatgcttttc
     2161 ctggcagagc agcctcctct gcctccccca cctctgctgc ctcccagcct caccaatgga
     2221 accactatcc ccactgccaa gtccaccccc acactcatta agcaaagcca acccaagtct
     2281 gccagtgaga agtcacagcg cagcaagaag gccaaggagc tgaagccaaa ggtgaagaag
     2341 ctcaagtacc accagtacat ccccccggac cagaagcagg acaggggggc accccccatg
     2401 gactcatcct acgccaagat cctgcagcag cagcagctct tcctccagct gcagatcctc
     2461 aaccagcagc agcagcagca ccacaactac caggccatcc tgcctgcccc gccaaagtca
     2521 gcaggcgagg ccctgggaag cagcgggacc cccccagtac gcagcctctc cactaccaat
     2581 agcagctcca gctcgggcgc ccctgggccc tgtgggctgg cacgtcagaa cagcacctca
     2641 ctgactggca agccgggagc cctgccggcc aacctggacg acatgaaggt ggcagagctg
     2701 aagcaggagc tgaagttgcg atcactgcct gtctcgggca ccaaaactga gctgattgag
     2761 cgccttcgag cctatcaaga ccaaatcagc cctgtgccag gagcccccaa ggcccctgcc
     2821 gccacctcta tcctgcacaa ggctggcgag gtggtggtag ccttcccagc ggcccggctg
     2881 agcacggggc cagccctggt ggcagcaggc ctggctccag ctgaggtggt ggtggccacg
     2941 gtggccagca gtggggtggt gaagtttggc agcacgggct ccacgccccc cgtgtctccc
     3001 accccctcgg agcgctcact gctcagcacg ggcgatgaaa actccacccc cggggacacc
     3061 tttggtgaga tggtgacatc acctctgacg cagctgaccc tgcaggcctc gccactgcag
     3121 atcctcgtga aggaggaggg cccccgggcc gggtcctgtt gcctgagccc tggggggcgg
     3181 gcggagctag aggggcgcga caaggaccag atgctgcagg agaaagacaa gcagatcgag
     3241 gcgctgacgc gcatgctccg gcagaagcag cagctggtgg agcggctcaa gctgcagctg
     3301 gagcaggaga agcgagccca gcagcccgcc cccgcccccg cccccctcgg cacccccgtg
     3361 aagcaggaga gcagcttctc cagctgccag ctgagccagc agcccctggg ccccgctcac
     3421 ccattcaacc ccagcctggc ggccccagcc accaaccaca tagacccttg tgctgtggcc
     3481 ccggggcccc cgtccgtggt ggtgaagcag gaagccttgc agcctgagcc cgagccggtc
     3541 cccgcccccc agttgcttct ggggcctcag ggccccagcc tcatcaaggg ggttgcacct
     3601 cccaccctca tcaccgactc cacagggacc caccttgtcc tcaccgtgac caataagaat
     3661 gcagacagcc ctggcctgtc cagtgggagc ccccagcagc cctcgtccca gcctggctct
     3721 ccagcgcctg ccccctctgc ccagatggac ctggagcacc cactgcagcc cctctttggg
     3781 gcccccactt ctctgctgaa gaaggaacca cctggctatg aggaagccat gagccagcag
     3841 cccaaacagc aggaaaatgg ttcctcaagc cagcagatgg acgacctgtt tgacattctc
     3901 attcagagcg gagaaatttc agcagatttc aaagagccgc catccctgcc agggaaggag
     3961 aagccatccc cgaagacagt ctgtgggtcc cccctggcag cacagccatc accttctgct
     4021 gagctccccc aggctgcccc acctcctcca ggctcaccct ccctccctgg acgcctggag
     4081 gacttcctgg agagcagcac ggggctgccc ctgctgacca gtgggcatga cgggccagag
     4141 cccctttccc tcattgacga cctccatagc cagatgctga gcagcactgc catcctggac
     4201 caccccccgt cacccatgga cacctcggaa ttgcactttg ttcctgagcc cagcagcacc
     4261 atgggcctgg acctggctga tggccacctg gacagcatgg actggctgga gctgtcgtca
     4321 ggtggtcccg tgctgagcct agcccccctc agcaccacag cccccagcct cttctccaca
     4381 gacttcctcg atggccatga tttgcagctg cactgggatt cctgcttgta gtctagaggg
     4441 cccgtttaaa cccgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt
     4501 tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa
     4561 taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg
     4621 gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg
     4681 gtgggctcta tggcttctga ggcggaaaga accagctggg gctctagggg gtatccccac
     4741 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
     4801 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
     4861 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
     4921 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca
     4981 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
     5041 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa
     5101 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
     5161 gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag
     5221 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc
     5281 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag
     5341 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
     5401 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctct gcctctgagc
     5461 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctcccgg
     5521 gagcttgtat atccattttc ggatctgatc aagagacagg atgaggatcg tttcgcatga
     5581 ttgaacaaga tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct
     5641 atgactgggc acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc
     5701 aggggcgccc ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcagg
     5761 acgaggcagc gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg
     5821 acgttgtcac tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc
     5881 tcctgtcatc tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc
     5941 ggctgcatac gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg
     6001 agcgagcacg tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc
     6061 atcaggggct cgcgccagcc gaactgttcg ccaggctcaa ggcgcgcatg cccgacggcg
     6121 aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc
     6181 gcttttctgg attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag
     6241 cgttggctac ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg
     6301 tgctttacgg tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg
     6361 agttcttctg agcgggactc tggggttcga aatgaccgac caagcgacgc ccaacctgcc
     6421 atcacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt
     6481 ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca
     6541 ccccaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
     6601 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
     6661 atcttatcat gtctgtatac cgtcgacctc tagctagagc ttggcgtaat catggtcata
     6721 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
     6781 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
     6841 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
     6901 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
     6961 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     7021 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     7081 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     7141 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     7201 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     7261 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     7321 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     7381 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     7441 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     7501 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
     7561 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     7621 ttgatccggc aaacaaacca ccgctggtag cggttttttt gtttgcaagc agcagattac
     7681 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     7741 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     7801 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
     7861 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
     7921 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
     7981 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
     8041 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
     8101 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
     8161 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
     8221 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
     8281 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
     8341 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
     8401 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
     8461 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
     8521 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
     8581 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
     8641 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
     8701 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
     8761 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
     8821 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgt
//
