LOCUS       Exported                9318 bp DNA     circular SYN 12-OCT-2021
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9318)
  AUTHORS   Sohum Mehta
  TITLE     Direct Submission
  JOURNAL   Exported Jan 18, 2022 from SnapGene 6.0.0
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9318
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
                     /note="color: #ffffff"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
                     /note="color: #ffffff; direction: RIGHT"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /note="color: #ffffff; direction: RIGHT"
     CDS             919..1617
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP(I174A)
                     /note="mammalian codon-optimized"
                     /note="color: #3366ff"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     CDS             1636..4875
                     /codon_start=1
                     /label=mouse MRTF-B
                     /note="color: #005493"
                     /translation="MIDSSKKQPQGFPEILTAEDFEPFKEKECLEGSNQKSLKEVLQLR
                     LQQRRTREQLVDQGIMPPLKSPAAFHEQIKSLERARTENFLKHKIRSRPDRSELVRMHI
                     LEETFAEPSLQATQMKLKRARLADDLNEKIAQRPGPMELVEKNILPVDSSVKEAIIGVV
                     KEDYPHTHGEFSFDEDSSDALSPDQPASQESQGSAASPSEPKVSASPPPVTASTPAQFT
                     SVSPAVPEFLKTPLTADQPPTRSTAPVLPTNTVSSAKSGPMLVKQSHPKNPNDKHRSKK
                     CKDPKPRVKKLKYHQYIPPNQKGEKSEPQMDSNYARLLQQQQLFLQLQILSQQQQQQQQ
                     QHYNYQTILPAPIKTDKNSSSGSNSGSSSSMPARRPGPLPSSLDDLKVSELKTELKLRG
                     LPVSGTKPDLIERLKPYQEVTSSNLATGSIVAVSSATIVTSNPEVTVALPVTTLHNAVT
                     SSVSTFKADLALPATSSVPHVENAHSPLPISPSPSEQSSLSTDDTNMTDTFTEIMTMMS
                     PSQLLCSSPLRVVSHDDSLSPSSSTLSTLELDAAEKDRKLQEKEKQIEELKRKLEQEQK
                     LVEVLKMQLEVEKRGQQRPPDPQPSDPPHPFNTSDPKHGSVGSSIKDEASLPDCSSPQQ
                     PITVPGHSVGQPISTGSQTLVAKKTVVVKQEVPMAQAEQQNVVSQFYLSSQGQPPALVA
                     QPQALLTTQTTQLLLPVSIQGSNVTSVQLPVGSLQLQTPAQGRVQAQPHVAAATQVPAA
                     ALPSALTSALPQKQEAFPQHVLGQPQPVRKVFTNSAPNTVLQYQRQPGPTNQQPFVSKT
                     SNPALQSRTAPLAPLQNGPSLASKPSSPPPPQQFVVQHSLFATPITKTKDPPRYEEAIK
                     QARSTQPALPEVSSVHSQQMDDLFDILIKSGEISFPIKEEPSPISKMKPVTASITTMPV
                     NTVVSRPPPQVQIAPPVSLEPVNSLSASLENQLEAFLDGTLPSATDTGPLQNSSEDRES
                     FSLIEDLQNDLLSHSSMLYQSHSPMETSEAQLVSGTPCLSLDLSDSNLDNMEWLDITMP
                     TTSSGLTPLSTTAPSMFSADFLDPQDLPLPWD"
     polyA_signal    4919..5143
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
                     /note="color: #a6acb3"
     rep_origin      5189..5617
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
     promoter        5631..5960
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
                     /note="color: #ffffff; direction: RIGHT"
     rep_origin      5811..5946
                     /label=SV40 ori
                     /note="SV40 origin of replication"
                     /note="color: #ffff00"
     CDS             6027..6821
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /note="color: #ccffcc"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    6995..7116
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
                     /note="color: #a6acb3"
     primer_bind     complement(7165..7181)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: LEFT"
     protein_bind    7189..7205
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #31849b"
     promoter        complement(7213..7243)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
                     /note="This reverse directional feature has 3 segments:
                        1: 7213 .. 7219 / #ffffff / -10
                        2: 7220 .. 7237 / #ffffff
                        3: 7238 .. 7243 / #ffffff / -35"
     protein_bind    7258..7279
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
                     /note="color: #31849b"
     rep_origin      complement(7567..8152)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     CDS             complement(8323..9183)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 8323 .. 9114 / #ccffcc
                        2: 9115 .. 9183 / #ccffcc / signal sequence
                     Cleavage site after base 9114"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(9184..9288)
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: LEFT"
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaactt aagcttggat gagcgagctg attaaggaga acatgcacat gaagctgtac
      961 atggagggca ccgtggacaa ccatcacttc aagtgcacat ccgagggcga aggcaagccc
     1021 tacgagggca cccagaccat gagaatcaag gtggtcgagg gcggccctct ccccttcgcc
     1081 ttcgacatcc tggctactag cttcctctac ggcagcaaga ccttcatcaa ccacacccag
     1141 ggcatccccg acttcttcaa gcagtccttc cctgagggct tcacatggga gagagtcacc
     1201 acatacgaag acgggggcgt gctgaccgct acccaggaca ccagcctcca ggacggctgc
     1261 ctcatctaca acgtcaagat cagaggggtg aacttcacat ccaacggccc tgtgatgcag
     1321 aagaaaacac tcggctggga ggccttcacc gagacgctgt accccgctga cggcggcctg
     1381 gaaggcagaa acgacatggc cctgaagctc gtgggcggga gccatctgat cgcaaacgcc
     1441 aagaccacat atagatccaa gaaacccgct aagaacctca agatgcctgg cgtctactat
     1501 gtggactaca gactggaaag aatcaaggag gccaacaacg agacctacgt cgagcagcac
     1561 gaggtggcag tggccagata ctgcgacctc cctagcaaac tggggcacaa gcttaatgga
     1621 ggtactggtg gaagtatgat cgatagctcc aagaagcagc cacaaggctt cccagaaatt
     1681 ttaactgctg aggattttga acctttcaaa gaaaaggaat gccttgaggg aagcaaccag
     1741 aaaagtctca aggaagtgct ccagctgagg ctgcaacaaa ggaggacaag agaacaacta
     1801 gtggaccagg gcatcatgcc acctttgaag agtccagcag cattccatga gcagataaaa
     1861 agcctggagc gagccagaac tgagaatttt ctgaagcaca agatccggag ccgcccagac
     1921 cgctctgagc ttgtcaggat gcatatttta gaagaaacgt ttgcagagcc atctcttcag
     1981 gccacacaga tgaagctgaa gagagctcgg ctggcagatg acctgaacga gaagattgct
     2041 caaaggcctg gtcccatgga gctggtggag aagaacatcc ttcctgtgga ttcgagtgtc
     2101 aaggaagcga ttataggtgt ggtgaaggag gactatcctc acactcatgg cgagttctca
     2161 tttgatgaag atagcagtga tgctttgtct ccagaccagc cagcgagcca ggagtctcag
     2221 ggctcagctg catcccccag tgagccaaaa gtcagtgcct cgccacctcc tgtgactgca
     2281 agcactccag cccagtttac ctcagtgtcc ccagcagttc ctgaattctt gaaaacccct
     2341 ctaactgcag accagcctcc cacaaggtct acagctcccg tgctccctac aaacactgtg
     2401 tcctcagcga agtctggccc aatgctggtg aagcaaagcc atcccaagaa tccaaacgac
     2461 aaacaccgta gcaaaaaatg caaagatccg aaaccgcgag tgaagaagct taagtaccac
     2521 cagtacattc cacccaacca gaagggggag aagagcgagc cacagatgga ctccaactat
     2581 gcccgcctgc tccagcagca gcagctcttc ctacagctgc agatcctgag ccagcagcag
     2641 cagcaacagc agcagcagca ctacaactac cagaccatcc tacctgcacc catcaaaact
     2701 gacaagaaca gcagcagcgg cagtaacagc ggcagtagca gcagcatgcc ggcgaggagg
     2761 ccaggaccgc tgccctccag cttggatgac ttaaaggtgt cagagctgaa gacagaactc
     2821 aaactgaggg gtctgccagt gtcaggaact aaaccagacc tcattgaacg cctgaaaccc
     2881 taccaggaag tgaccagtag taaccttgcc actggcagta ttgtggcagt gtcatcagca
     2941 accattgtca ccagtaaccc agaggtcaca gtggcactgc cagtcacaac actgcataat
     3001 gctgtaacaa gctctgtgtc cactttcaag gcagacttgg cacttcctgc aaccagcagt
     3061 gtgccccatg ttgaaaatgc ccactctcct ctgcccatct ccccatcacc ctctgagcag
     3121 tccagtctca gtaccgatga cacaaacatg acagacacct tcacagagat catgactatg
     3181 atgtcacctt cccagttgtt gtgttcatct cccctgaggg tggtgagcca tgatgacagc
     3241 ctgagccctt ccagtagcac cctgtctact ctagaactgg atgctgctga gaaggaccgc
     3301 aaacttcagg agaaggagaa acagattgaa gaactgaaga ggaaactgga acaagaacag
     3361 aagcttgtgg aggtcctcaa gatgcagctt gaggtggaaa aacgtgggca gcagcgacca
     3421 ccagaccctc agcccagtga tcccccacat ccctttaata catcagatcc taaacatggc
     3481 agtgttgggt cctccatcaa ggatgaggcc tcactccctg actgctccag cccccagcag
     3541 cccatcacag tgcctggcca ctctgtaggc cagcccatct ccacgggtag ccagaccctt
     3601 gttgccaaaa agactgtggt cgtcaagcaa gaggtcccca tggcccaagc agagcagcag
     3661 aacgttgtct cgcagttcta ccttagttcc cagggacagc cacctgccct tgttgctcag
     3721 cctcaggcct tactgaccac acagacaact cagctgctgc tgccagtgtc catccagggc
     3781 tcaaatgtta cttcagtgca actcccagta ggcagccttc aactccagac cccagcacaa
     3841 ggaagagtcc aggctcagcc tcatgtagct gctgccacac aggttccagc tgcagccctg
     3901 ccctcagcct tgacctcagc actgcctcag aagcaggaag ccttcccaca gcacgtgttg
     3961 ggccagcctc agccagtcag aaaggtcttc acaaactcgg caccaaacac agttcttcag
     4021 tatcagaggc agcctggccc aacgaaccag cagccctttg tcagtaagac ctctaaccct
     4081 gctcttcaat ccagaactgc cccacttgca cccctgcaaa atggacccag cctagccagc
     4141 aagcctagct caccaccccc accccagcag tttgttgttc agcactcttt gtttgcaacc
     4201 ccaatcacga agacaaaaga tccacctcga tatgaggaag ccatcaagca ggcacgtagc
     4261 acacagccag cccttccaga ggtttccagt gtgcacagtc agcagatgga cgacctcttc
     4321 gacatcctca taaagagtgg agagatttcc ttcccaataa aagaagagcc ttctccaatt
     4381 tccaagatga aaccagtgac agccagcatc accacaatgc cagtcaatac cgtggtctct
     4441 cgaccaccgc cccaggtcca aatagcacca cctgtatcct tagaacctgt gaacagttta
     4501 tctgccagct tagagaacca gctagaagcc ttcttggatg gcactttgcc ctcggccact
     4561 gacactggcc cactgcagaa cagcagtgaa gatagagagt ctttctctct gatcgaggat
     4621 ctgcagaacg acttgctgag tcattccagc atgctgtacc agtctcactc tcccatggag
     4681 acctctgagg cccagttggt gtcagggacc ccctgtctat ctcttgacct gtcagactcc
     4741 aacctggaca acatggagtg gctagacatt accatgccca ccacatcctc cgggctcact
     4801 ccactgagta ccactgcgcc aagcatgttc tctgctgatt ttctggaccc acaggacctg
     4861 ccgctgccat gggacttgta gtctagaggg cccgtttaaa cccgctgatc agcctcgact
     4921 gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg
     4981 gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg
     5041 agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg
     5101 gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctga ggcggaaaga
     5161 accagctggg gctctagggg gtatccccac gcgccctgta gcggcgcatt aagcgcggcg
     5221 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
     5281 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
     5341 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
     5401 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
     5461 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
     5521 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta
     5581 aaaaatgagc tgatttaaca aaaatttaac gcgaattaat tctgtggaat gtgtgtcagt
     5641 tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
     5701 attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
     5761 gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc
     5821 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg
     5881 cagaggccga ggccgcctct gcctctgagc tattccagaa gtagtgagga ggcttttttg
     5941 gaggcctagg cttttgcaaa aagctcccgg gagcttgtat atccattttc ggatctgatc
     6001 aagagacagg atgaggatcg tttcgcatga ttgaacaaga tggattgcac gcaggttctc
     6061 cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct
     6121 ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg
     6181 acctgtccgg tgccctgaat gaactgcagg acgaggcagc gcggctatcg tggctggcca
     6241 cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc
     6301 tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga
     6361 aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc
     6421 cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc
     6481 ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg
     6541 ccaggctcaa ggcgcgcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct
     6601 gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc
     6661 tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc
     6721 ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc
     6781 agcgcatcgc cttctatcgc cttcttgacg agttcttctg agcgggactc tggggttcga
     6841 aatgaccgac caagcgacgc ccaacctgcc atcacgagat ttcgattcca ccgccgcctt
     6901 ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg
     6961 cggggatctc atgctggagt tcttcgccca ccccaacttg tttattgcag cttataatgg
     7021 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
     7081 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctgtatac cgtcgacctc
     7141 tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct
     7201 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
     7261 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
     7321 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
     7381 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     7441 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     7501 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     7561 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     7621 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     7681 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     7741 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     7801 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     7861 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     7921 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     7981 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
     8041 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     8101 cggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     8161 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     8221 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     8281 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     8341 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     8401 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     8461 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     8521 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     8581 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     8641 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     8701 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     8761 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     8821 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     8881 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
     8941 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     9001 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     9061 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     9121 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
     9181 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     9241 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
     9301 aaaagtgcca cctgacgt
//
