LOCUS       Exported               12967 bp DNA     circular SYN 21-JUL-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12967)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Jan 18, 2022 from SnapGene 6.0.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..12967
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             835..1015
                     /locus_tag="5' LTR"
                     /label=5' LTR
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1680..1913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2098..2142
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2440..2557
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2602..4346
                     /locus_tag="CAG"
                     /label=CAG
     enhancer        2663..2966
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2968..3245
                     /label=chicken beta-actin promoter
     intron          3247..4264
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     4328..4371
                     /label=PP023_Q6a-P2A_fwd
     regulatory      4353..4362
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4359..5117
                     /codon_start=1
                     /product="Archon_V4, W42G/M85I/F98L/V124G/W148C/A238S"
                     /label=Quasar-6a
                     /note="Archon_V4, W42G/M85I/F98L/V124G/W148C/A238S"
                     /translation="MVSIALQAGYDLLGDGRPESLWLGIGTLLMLIGTFYFLVRAGGET
                     DKDAREYYAVTILVSGIASAAYLSMFFGIGLTEVPVGGEILNIYYARYAQWLLTTPLLL
                     LHLALLAKVDRVTIGTLVGGDALMIVTGLIGTLSHTAIARYSWCLFSTICMIVVLYVLA
                     TSLRSAAKERGPEVASTFNILTALVLVLWTAYPIIWIIGTEGAGVVGLGIETLLFMVLD
                     VTAKVGFGFILLRSRSILQDTEAPEPSAGAD"
     CDS             5118..5177
                     /label=TS
                     /note="/ApEinfo_fwdcolor=""pink"
                     /note="/ApEinfo_revcolor=""pink"
                     /note="/ApEinfo_graphicformat=""arrow_data {{0 1 2 0 0 -1}
                     {} 0} width 5 offset 0"""
     CDS             5178..5888
                     /product="enhanced variant of YFP (Heikal et al., 2001)
                     "
                     /label=Dark mCitrine (Y66G)
                     /label=Citrine
                     /note="/ApEinfo_fwdcolor=""pink"
                     /note="/ApEinfo_revcolor=""pink"
                     /note="/ApEinfo_graphicformat=""arrow_data {{0 1 2 0 0 -1}
                     {} 0} width 5 offset 0"""
     CDS             5889..6086
                     /label=TSx3
                     /note="/ApEinfo_fwdcolor=""pink"
                     /note="/ApEinfo_revcolor=""pink"
                     /note="/ApEinfo_graphicformat=""arrow_data {{0 1 2 0 0 -1}
                     {} 0} width 5 offset 0"""
     CDS             6087..6107
                     /label=ER2
                     /note="/ApEinfo_fwdcolor=""pink"
                     /note="/ApEinfo_revcolor=""pink"""
                     /note="/ApEinfo_graphicformat=""arrow_data {{0 1 2 0 0 -1}
                     {} 0} width 5 offset 0"""
     CDS             6108..6173
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="GSGATNFSLLKQAGDVEENPGP"
     primer_bind     complement(6156..6183)
                     /label=PP023_Q6a-P2A_rev
     primer_bind     6164..6192
                     /label=PP023_jRGeCo1a-CAAX_fwd
     misc_feature    6174..6224
                     /locus_tag="NES"
                     /label=NES
     CDS             6237..6254
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     misc_feature    6258..7580
                     /locus_tag="jRGECO1a"
                     /label=jRGECO1a
     CDS             6258..6290
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             6294..6317
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     CDS             6345..6401
                     /codon_start=1
                     /product="calmodulin-binding peptide from avian smooth 
                     muscle myosin light chain kinase"
                     /label=calmodulin-binding peptide
                     /translation="RRKWNKAGHAVRAIGRLSS"
     misc_feature    7581..7631
                     /label=CAAX_motif
     primer_bind     complement(7613..7659)
                     /label=PP021_GCaMP-CAAX_rev
     misc_feature    7659..8247
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(8130..8141)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(8250..8266)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     LTR             8772..8952
                     /locus_tag="3' LTR"
                     /label=3' LTR
     polyA_signal    8984..9208
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      9254..9682
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        9696..10025
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9876..10011
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        10073..10120
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             10139..10513
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    10643..10764
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10813..10829)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    10837..10853
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(10861..10891)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10906..10927
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(11215..11803)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(11974..12834)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(12835..12939)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaatta actagttatt aatagtaatc aattacgggg tcattagttc
     2641 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
     2701 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
     2761 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
     2821 tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc
     2881 ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct
     2941 acgtattagt catcgctatt accatggtcg aggtgagccc cacgttctgc ttcactctcc
     3001 ccatctcccc cccctcccca cccccaattt tgtatttatt tattttttaa ttattttgtg
     3061 cagcgatggg ggcggggggg gggggggggc gcgcgccagg cggggcgggg cggggcgagg
     3121 ggcggggcgg ggcgaggcgg agaggtgcgg cggcagccaa tcagagcggc gcgctccgaa
     3181 agtttccttt tatggcgagg cggcggcggc ggcggcccta taaaaagcga agcgcgcggc
     3241 gggcggggag tcgctgcgac gctgccttcg ccccgtgccc cgctccgccg ccgcctcgcg
     3301 ccgcccgccc cggctctgac tgaccgcgtt actcccacag gtgagcgggc gggacggccc
     3361 ttctcctccg ggctgtaatt agcgcttggt ttaatgacgg cttgtttctt ttctgtggct
     3421 gcgtgaaagc cttgaggggc tccgggaggg ccctttgtgc ggggggagcg gctcgggggg
     3481 tgcgtgcgtg tgtgtgtgcg tggggagcgc cgcgtgcggc tccgcgctgc ccggcggctg
     3541 tgagcgctgc gggcgcggcg cggggctttg tgcgctccgc agtgtgcgcg aggggagcgc
     3601 ggccgggggc ggtgccccgc ggtgcggggg gggctgcgag gggaacaaag gctgcgtgcg
     3661 gggtgtgtgc gtgggggggt gagcaggggg tgtgggcgcg tcggtcgggc tgcaaccccc
     3721 cctgcacccc cctccccgag ttgctgagca cggcccggct tcgggtgcgg ggctccgtac
     3781 ggggcgtggc gcggggctcg ccgtgccggg cggggggtgg cggcaggtgg gggtgccggg
     3841 cggggcgggg ccgcctcggg ccggggaggg ctcgggggag gggcgcggcg gcccccggag
     3901 cgccggcggc tgtcgaggcg cggcgagccg cagccattgc cttttatggt aatcgtgcga
     3961 gagggcgcag ggacttcctt tgtcccaaat ctgtgcggag ccgaaatctg ggaggcgccg
     4021 ccgcaccccc tctagcgggc gcggggcgaa gcggtgcggc gccggcagga aggaaatggg
     4081 cggggagggc cttcgtgcgt cgccgcgccg ccgtcccctt ctccctctcc agcctcgggg
     4141 ctgtccgcgg ggggacggct gccttcgggg gggacggggc agggcggggt tcggcttctg
     4201 gcgtgtgacc ggcggctcta gagcctctgc taaccatgtt catgccttct tctttttcct
     4261 acagctcctg ggcaacgtgc tggttattgt gctgtctcat cattttggca aagaattgat
     4321 ttgataccgc gggcccggga tctcgaggat ccgccaccat ggtttctatc gcactgcagg
     4381 ctggttatga cctcctcgga gatggtagac ctgaaagtct gtggctcgga attggtacac
     4441 tgctgatgct gatcggaacc ttctacttcc tggttagagc gggcggagag actgacaagg
     4501 atgcacggga gtactacgca gtgacaatcc tggtcagcgg tattgccagt gcagcttatc
     4561 tgtcaatgtt ctttggtatt ggcctcacag aggtgccagt aggtggagag atcctgaaca
     4621 tctactacgc aagatacgca cagtggctgc tgactacacc actgttgctg ctgcacctgg
     4681 cactcctggc caaagttgat agagtgacca tcggcactct cgttggcggc gatgctctga
     4741 tgattgtcac aggcctgata gggacactgt cacatactgc cattgccaga tactcttggt
     4801 gcctgttctc aactatctgt atgatcgtcg ttctgtatgt cctggccact tctctgcggt
     4861 cagccgctaa ggagagaggc ccagaggttg cctcaacctt caacatcctc accgcactgg
     4921 ttctggtcct gtggactgct taccctatca tctggattat cggcaccgag ggtgccggag
     4981 ttgtgggcct gggaatcgaa actttgctgt tcatggtgct ggacgtcacc gccaaggtgg
     5041 gcttcggttt tatcctgctc aggtcaagaa gcattctgca agatacagaa gcacctgaac
     5101 cttcagctgg agctgataag agtagaatca caagcgaagg cgagtacatc cccctggatc
     5161 aaatagacat aaatgtagtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc
     5221 tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg
     5281 gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg
     5341 tgccctggcc caccctcgtg accaccttcg gcggcggcct gatgtgcttc gcccgctacc
     5401 ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg
     5461 agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg
     5521 agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca
     5581 acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg
     5641 acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca
     5701 gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc
     5761 tgcccgacaa ccactacctg agctaccagt ccgccctgag caaagacccc aacgagaagc
     5821 gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg
     5881 agctgtacaa atcaaggatc acctctgagg gcgagtatat ccctctggat cagatcgaca
     5941 ttaacgtcgg tggaaaatcc cgcataacct ctgagggcga gtacatccct ctggatcaaa
     6001 tcgatataaa cgtgggggga aaaagtcgaa tcacttctga gggcgagtat atacccctcg
     6061 atcagattga catcaatgtg gggggctttt gttatgagaa tgaagtgggc tccggagcca
     6121 cgaacttctc tctgttaaag caagcaggag acgtggaaga aaaccccggt cccctgcaga
     6181 acgagcttgc tcttaagttg gctggacttg atattaacaa gactggagga ggttctcatc
     6241 atcatcatca tcatggtatg gctagcatga ctggtggaca gcaaatgggt cgggatctgt
     6301 acgacgatga cgataaggat ctcgcaacaa tggtcgactc atcacgtcgt aagtggaata
     6361 aggcaggtca cgcagtcaga gctataggtc ggctgagctc acccgtggtt tccgagcgga
     6421 tgtaccccga ggacggcgcc ctgaagagcg agatcaagaa ggggctgagg ctgaaggacg
     6481 gcggccacta cgccgccgag gtcaagacca cctacaaggc caagaagccc gtgcagctgc
     6541 ccggcgccta catcgtggac atcaagttgg acatcgtgtc ccacaacgag gactacacca
     6601 tcgtggaaca gtgcgaacgc gccgagggcc gccactccac cggcggcatg gacgagctgt
     6661 acaagggagg tacaggcggg agtctggtga gcaagggcga ggaggataac atggccatca
     6721 tcaaggagtt catgcgcttc aaggtgcaca tggagggctc cgtgaacggc cacgagttcg
     6781 agatcgaggg cgagggcgag ggccgcccct acgaggcctt tcagaccgct aagctgaagg
     6841 tgaccaaggg tggccccctg cccttcgcct gggacatcct gtcccctcag ttcatgtacg
     6901 gctccaaggc ctacattaag cacccagccg acatccccga ctacttcaag ctgtccttcc
     6961 ccgagggctt caggtgggag cgcgtgatga acttcgagga cggcggcatt attcacgtga
     7021 accaggactc ctccctgcag gacggcgtat tcatctacaa ggtgaagctg cgcggcacca
     7081 acttcccccc cgacggcccc gtaatgcaga agaagaccat gggctgggag gctacgcgtg
     7141 acgacctgac tgaagagcag atcgcagaat ttaaagaggc tttctcccta tttgacaagg
     7201 acggggatgg gacgataaca accaaggagc ttgggacggt gttccgatcg ctggggcaga
     7261 accccacaga agcagagctg caggacatga tcaatgaagt agatgccgac ggtgacggca
     7321 cattcgactt ccctgagttc ctgacgatga tggcaagaaa aatgaatgac acagacagtg
     7381 aagaggaaat tcgcgaagcg ttccgcgtgt ttgataagga cggcaatggc tacatcggcg
     7441 cagcagagct tcgccacgtg atgacagacc ttggagagaa gttaacagat gaggaggttg
     7501 atgaaatgat cagggtagca gacatcgatg gggatggtca ggtaaactac gaagagtttg
     7561 tacaaatgat gacagcgaag accggtggta aaaagaagaa aaagaagtca aagacaaagt
     7621 gtgtaattat gtgagaattc gatatcaagc ttatcgataa tcaacctctg gattacgaaa
     7681 tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg
     7741 ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct
     7801 tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg
     7861 gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct
     7921 gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg
     7981 ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg
     8041 tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc acctggattc
     8101 tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc
     8161 gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc
     8221 ggatctccct ttgggccgcc tccccgcatc gataccgtcg acctcgagac ctagaaaaac
     8281 atggagcaat cacaagtagc aatacagcag ctaccaatgc tgattgtgcc tggctagaag
     8341 cacaagagga ggaggaggtg ggttttccag tcacacctca ggtaccttta agaccaatga
     8401 cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc
     8461 taattcactc ccaacgaaga caagatatcc ttgatctgtg gatctaccac acacaaggct
     8521 acttccctga ttggcagaac tacacaccag ggccagggat cagatatcca ctgacctttg
     8581 gatggtgcta caagctagta ccagttgagc aagagaaggt agaagaagcc aatgaaggag
     8641 agaacacccg cttgttacac cctgtgagcc tgcatgggat ggatgacccg gagagagaag
     8701 tattagagtg gaggtttgac agccgcctag catttcatca catggcccga gagctgcatc
     8761 cggactgtac tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac
     8821 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg
     8881 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga
     8941 aaatctctag cagggcccgt ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc
     9001 cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc
     9061 actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct
     9121 attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg
     9181 catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaaccag ctggggctct
     9241 agggggtatc cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
     9301 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
     9361 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
     9421 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt
     9481 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
     9541 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat
     9601 tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt
     9661 taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt
     9721 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
     9781 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
     9841 agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt
     9901 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg
     9961 cctctgcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt
    10021 gcaaaaagct cccgggagct tgtatatcca ttttcggatc tgatcagcac gtgttgacaa
    10081 ttaatcatcg gcatagtata tcggcatagt ataatacgac aaggtgagga actaaaccat
    10141 ggccaagttg accagtgccg ttccggtgct caccgcgcgc gacgtcgccg gagcggtcga
    10201 gttctggacc gaccggctcg ggttctcccg ggacttcgtg gaggacgact tcgccggtgt
    10261 ggtccgggac gacgtgaccc tgttcatcag cgcggtccag gaccaggtgg tgccggacaa
    10321 caccctggcc tgggtgtggg tgcgcggcct ggacgagctg tacgccgagt ggtcggaggt
    10381 cgtgtccacg aacttccggg acgcctccgg gccggccatg accgagatcg gcgagcagcc
    10441 gtgggggcgg gagttcgccc tgcgcgaccc ggccggcaac tgcgtgcact tcgtggccga
    10501 ggagcaggac tgacacgtgc tacgagattt cgattccacc gccgccttct atgaaaggtt
    10561 gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat
    10621 gctggagttc ttcgcccacc ccaacttgtt tattgcagct tataatggtt acaaataaag
    10681 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
    10741 gtccaaactc atcaatgtat cttatcatgt ctgtataccg tcgacctcta gctagagctt
    10801 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
    10861 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
    10921 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
    10981 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
    11041 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
    11101 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
    11161 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
    11221 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
    11281 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
    11341 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
    11401 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
    11461 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
    11521 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
    11581 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
    11641 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
    11701 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
    11761 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
    11821 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
    11881 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
    11941 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
    12001 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
    12061 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
    12121 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
    12181 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
    12241 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
    12301 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
    12361 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
    12421 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
    12481 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
    12541 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
    12601 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
    12661 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
    12721 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
    12781 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
    12841 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
    12901 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
    12961 acctgac
//
