LOCUS       Exported                9495 bp ds-DNA     circular SYN 16-MAY-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9495)
  AUTHORS   locusliu
  TITLE     Direct Submission
  JOURNAL   Exported Monday, May 16, 2022 from SnapGene Viewer 5.1.7
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9495
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        complement(699..776)
                     /gene="lacI"
                     /label=lacI promoter
     promoter        1085..1103
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    1104..1128
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             1143..1165
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             1176..1202
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     CDS             1221..5138
                     /codon_start=1
                     /product="CRISPR-associated protein from Acidaminococcus 
                     sp. BV3L6 (Zetsche et al., 2015)"
                     /label=AsCpf1
                     /translation="TQFEGFTNLYQVSKTLRFELIPQGKTLKHIQEQGFIEEDKARNDH
                     YKELKPIIDRIYKTYADQCLQLVQLDWENLSAAIDSYRKEKTEETRNALIEEQATYRNA
                     IHDYFIGRTDNLTDAINKRHAEIYKGLFKAELFNGKVLKQLGTVTTTEHENALLRSFDK
                     FTTYFSGFYENRKNVFSAEDISTAIPHRIVQDNFPKFKENCHIFTRLITAVPSLREHFE
                     NVKKAIGIFVSTSIEEVFSFPFYNQLLTQTQIDLYNQLLGGISREAGTEKIKGLNEVLN
                     LAIQKNDETAHIIASLPHRFIPLFKQILSDRNTLSFILEEFKSDEEVIQSFCKYKTLLR
                     NENVLETAEALFNELNSIDLTHIFISHKKLETISSALCDHWDTLRNALYERRISELTGK
                     ITKSAKEKVQRSLKHEDINLQEIISAAGKELSEAFKQKTSEILSHAHAALDQPLPTTLK
                     KQEEKEILKSQLDSLLGLYHLLDWFAVDESNEVDPEFSARLTGIKLEMEPSLSFYNKAR
                     NYATKKPYSVEKFKLNFQMPTLASGWDVNKEKNNGAILFVKNGLYYLGIMPKQKGRYKA
                     LSFEPTEKTSEGFDKMYYDYFPDAAKMIPKCSTQLKAVTAHFQTHTTPILLSNNFIEPL
                     EITKEIYDLNNPEKEPKKFQTAYAKKTGDQKGYREALCKWIDFTRDFLSKYTKTTSIDL
                     SSLRPSSQYKDLGEYYAELNPLLYHISFQRIAEKEIMDAVETGKLYLFQIYNKDFAKGH
                     HGKPNLHTLYWTGLFSPENLAKTSIKLNGQAELFYRPKSRMKRMAHRLGEKMLNKKLKD
                     QKTPIPDTLYQELYDYVNHRLSHDLSDEARALLPNVITKEVSHEIIKDRRFTSDKFFFH
                     VPITLNYQAANSPSKFNQRVNAYLKEHPETPIIGIDRGERNLIYITVIDSTGKILEQRS
                     LNTIQQFDYQKKLDNREKERVAARQAWSVVGTIKDLKQGYLSQVIHEIVDLMIHYQAVV
                     VLENLNFGFKSKRTGIAEKAVYQQFEKMLIDKLNCLVLKDYPAEKVGGVLNPYQLTDQF
                     TSFAKMGTQSGFLFYVPAPYTSKIDPLTGFVDPFVWKTIKNHESRKHFLEGFDFLHYDV
                     KTGDFILHFKMNRNLSFQRGLPGFMPAWDIVFEKNETQFDAKGTPFIAGKRIVPVIENH
                     RFTGRYRDLYPANELIALLEEKGIVFRDGSNILPKLLENDDSHAIDTMVALIRSVLQMR
                     NSNAATGEDYINSPVRDLNGVCFDSRFQNPEWPMDADANGAYHIALKGQLLLNHLKESK
                     DLKLQNGISNQDWLAYIQELRN"
     regulatory      4858..4867
                     /regulatory_class="other"
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             5139..5186
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             5193..5273
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
     CDS             5274..5300
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     CDS             5307..5324
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      5391..5438
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      5475..5930
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        5957..6061
                     /gene="bla"
                     /label=AmpR promoter
     CDS             6062..6922
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      7093..7681
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    7867..8009
                     /label=bom
                     /note="basis of mobility region from pBR322"
     CDS             complement(8111..8302)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(join(9111..9495,1..698))
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
ORIGIN
        1 agcatttgca tggtttgttg aaaaccggac atggcactcc agtcgccttc ccgttccgct
       61 atcggctgaa tttgattgcg agtgagatat ttatgccagc cagccagacg cagacgcgcc
      121 gagacagaac ttaatgggcc cgctaacagc gcgatttgct ggtgacccaa tgcgaccaga
      181 tgctccacgc ccagtcgcgt accgtcttca tgggagaaaa taatactgtt gatgggtgtc
      241 tggtcagaga catcaagaaa taacgccgga acattagtgc aggcagcttc cacagcaatg
      301 gcatcctggt catccagcgg atagttaatg atcagcccac tgacgcgttg cgcgagaaga
      361 ttgtgcaccg ccgctttaca ggcttcgacg ccgcttcgtt ctaccatcga caccaccacg
      421 ctggcaccca gttgatcggc gcgagattta atcgccgcga caatttgcga cggcgcgtgc
      481 agggccagac tggaggtggc aacgccaatc agcaacgact gtttgcccgc cagttgttgt
      541 gccacgcggt tgggaatgta attcagctcc gccatcgccg cttccacttt ttcccgcgtt
      601 ttcgcagaaa cgtggctggc ctggttcacc acgcgggaaa cggtctgata agagacaccg
      661 gcatactctg cgacatcgta taacgttact ggtttcacat tcaccaccct gaattgactc
      721 tcttccgggc gctatcatgc cataccgcga aaggttttgc gccattcgat ggtgtccggg
      781 atctcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag
      841 gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc
      901 cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg
      961 gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt
     1021 ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg
     1081 aaattaatac gactcactat aggggaattg tgagcggata acaattcccc tctagaaata
     1141 attttgttta actttaagaa ggagatatac atatgcccgc cgccaagcgc gtgaagctgg
     1201 acggcggtag cggcggaggc acacagttcg agggctttac caacctgtat caggtgagca
     1261 agacactgcg gtttgagctg atcccacagg gcaagaccct gaagcacatc caggagcagg
     1321 gcttcatcga ggaggacaag gcccgcaatg atcactacaa ggagctgaag cccatcatcg
     1381 atcggatcta caagacctat gccgaccagt gcctgcagct ggtgcagctg gattgggaga
     1441 acctgagcgc cgccatcgac tcctatagaa aggagaaaac cgaggagaca aggaacgccc
     1501 tgatcgagga gcaggccaca tatcgcaatg ccatccacga ctacttcatc ggccggacag
     1561 acaacctgac cgatgccatc aataagagac acgccgagat ctacaagggc ctgttcaagg
     1621 ccgagctgtt taatggcaag gtgctgaagc agctgggcac cgtgaccaca accgagcacg
     1681 agaacgccct gctgcggagc ttcgacaagt ttacaaccta cttctccggc ttttatgaga
     1741 acaggaagaa cgtgttcagc gccgaggata tcagcacagc catcccacac cgcatcgtgc
     1801 aggacaactt ccccaagttt aaggagaatt gtcacatctt cacacgcctg atcaccgccg
     1861 tgcccagcct gcgggagcac tttgagaacg tgaagaaggc catcggcatc ttcgtgagca
     1921 cctccatcga ggaggtgttt tccttccctt tttataacca gctgctgaca cagacccaga
     1981 tcgacctgta taaccagctg ctgggaggaa tctctcggga ggcaggcacc gagaagatca
     2041 agggcctgaa cgaggtgctg aatctggcca tccagaagaa tgatgagaca gcccacatca
     2101 tcgcctccct gccacacaga ttcatccccc tgtttaagca gatcctgtcc gataggaaca
     2161 ccctgtcttt catcctggag gagtttaaga gcgacgagga agtgatccag tccttctgca
     2221 agtacaagac actgctgaga aacgagaacg tgctggagac agccgaggcc ctgtttaacg
     2281 agctgaacag catcgacctg acacacatct tcatcagcca caagaagctg gagacaatca
     2341 gcagcgccct gtgcgaccac tgggatacac tgaggaatgc cctgtatgag cggagaatct
     2401 ccgagctgac aggcaagatc accaagtctg ccaaggagaa ggtgcagcgc agcctgaagc
     2461 acgaggatat caacctgcag gagatcatct ctgccgcagg caaggagctg agcgaggcct
     2521 tcaagcagaa aaccagcgag atcctgtccc acgcacacgc cgccctggat cagccactgc
     2581 ctacaaccct gaagaagcag gaggagaagg agatcctgaa gtctcagctg gacagcctgc
     2641 tgggcctgta ccacctgctg gactggtttg ccgtggatga gtccaacgag gtggaccccg
     2701 agttctctgc ccggctgacc ggcatcaagc tggagatgga gccttctctg agcttctaca
     2761 acaaggccag aaattatgcc accaagaagc cctactccgt ggagaagttc aagctgaact
     2821 ttcagatgcc tacactggcc tctggctggg acgtgaataa ggagaagaac aatggcgcca
     2881 tcctgtttgt gaagaacggc ctgtactatc tgggcatcat gccaaagcag aagggcaggt
     2941 ataaggccct gagcttcgag cccacagaga aaaccagcga gggctttgat aagatgtact
     3001 atgactactt ccctgatgcc gccaagatga tcccaaagtg cagcacccag ctgaaggccg
     3061 tgacagccca ctttcagacc cacacaaccc ccatcctgct gtccaacaat ttcatcgagc
     3121 ctctggagat cacaaaggag atctacgacc tgaacaatcc tgagaaggag ccaaagaagt
     3181 ttcagacagc ctacgccaag aaaaccggcg accagaaggg ctacagagag gccctgtgca
     3241 agtggatcga cttcacaagg gattttctgt ccaagtatac caagacaacc tctatcgatc
     3301 tgtctagcct gcggccatcc tctcagtata aggacctggg cgagtactat gccgagctga
     3361 atcccctgct gtaccacatc agcttccaga gaatcgccga gaaggagatc atggatgccg
     3421 tggagacagg caagctgtac ctgttccaga tctataacaa ggactttgcc aagggccacc
     3481 acggcaagcc taatctgcac acactgtatt ggaccggcct gttttctcca gagaacctgg
     3541 ccaagacaag catcaagctg aatggccagg ccgagctgtt ctaccgccct aagtccagga
     3601 tgaagaggat ggcacaccgg ctgggagaga agatgctgaa caagaagctg aaggatcaga
     3661 aaaccccaat ccccgacacc ctgtaccagg agctgtacga ctatgtgaat cacagactgt
     3721 cccacgacct gtctgatgag gccagggccc tgctgcccaa cgtgatcacc aaggaggtgt
     3781 ctcacgagat catcaaggat aggcgcttta ccagcgacaa gttctttttc cacgtgccta
     3841 tcacactgaa ctatcaggcc gccaattccc catctaagtt caaccagagg gtgaatgcct
     3901 acctgaagga gcaccccgag acacctatca tcggcatcga tcggggcgag agaaacctga
     3961 tctatatcac agtgatcgac tccaccggca agatcctgga gcagcggagc ctgaacacca
     4021 tccagcagtt tgattaccag aagaagctgg acaacaggga gaaggagagg gtggcagcaa
     4081 ggcaggcctg gtctgtggtg ggcacaatca aggatctgaa gcagggctat ctgagccagg
     4141 tcatccacga gatcgtggac ctgatgatcc actaccaggc cgtggtggtg ctggagaacc
     4201 tgaatttcgg ctttaagagc aagaggaccg gcatcgccga gaaggccgtg taccagcagt
     4261 tcgagaagat gctgatcgat aagctgaatt gcctggtgct gaaggactat ccagcagaga
     4321 aagtgggagg cgtgctgaac ccataccagc tgacagacca gttcacctcc tttgccaaga
     4381 tgggcaccca gtctggcttc ctgttttacg tgcctgcccc atatacatct aagatcgatc
     4441 ccctgaccgg cttcgtggac cccttcgtgt ggaaaaccat caagaatcac gagagccgca
     4501 agcacttcct ggagggcttc gactttctgc actacgacgt gaaaaccggc gacttcatcc
     4561 tgcactttaa gatgaacaga aatctgtcct tccagagggg cctgcccggc tttatgcctg
     4621 catgggatat cgtgttcgag aagaacgaga cacagtttga cgccaagggc acccctttca
     4681 tcgccggcaa gagaatcgtg ccagtgatcg agaatcacag attcaccggc agataccggg
     4741 acctgtatcc tgccaacgag ctgatcgccc tgctggagga gaagggcatc gtgttcaggg
     4801 atggctccaa catcctgcca aagctgctgg agaatgacga ttctcacgcc atcgacacca
     4861 tggtggccct gatccgcagc gtgctgcaga tgcggaactc caatgccgcc acaggcgagg
     4921 actatatcaa cagccccgtg cgcgatctga atggcgtgtg cttcgactcc cggtttcaga
     4981 acccagagtg gcccatggac gccgatgcca atggcgccta ccacatcgcc ctgaagggcc
     5041 agctgctgct gaatcacctg aaggagagca aggatctgaa gctgcagaac ggcatctcca
     5101 atcaggactg gctggcctac atccaggagc tgcgcaacaa aaggccggcg gccacgaaaa
     5161 aggccggcca ggcaaaaaag aaaaagggat cctacccata cgatgttcca gattacgctt
     5221 atccctacga cgtgcctgat tatgcatacc catatgatgt ccccgactat gccccggcag
     5281 ctaagagggt gaaactggat ctcgagcacc accaccacca ccactgagat ccggctgcta
     5341 acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac
     5401 cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg
     5461 gattggcgaa tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac
     5521 gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc
     5581 ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt
     5641 agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg
     5701 ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac
     5761 gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta
     5821 ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat
     5881 ttaacaaaaa tttaacgcga attttaacaa aatattaacg tttacaattt caggtggcac
     5941 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
     6001 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
     6061 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
     6121 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
     6181 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
     6241 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
     6301 ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
     6361 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
     6421 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
     6481 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
     6541 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
     6601 gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
     6661 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
     6721 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
     6781 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
     6841 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
     6901 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
     6961 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
     7021 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     7081 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     7141 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     7201 ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt
     7261 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     7321 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     7381 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     7441 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     7501 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     7561 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     7621 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     7681 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     7741 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
     7801 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
     7861 agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata
     7921 tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc
     7981 tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc
     8041 cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga
     8101 gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa
     8161 gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct
     8221 cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg
     8281 cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg
     8341 taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg
     8401 cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga
     8461 gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg
     8521 gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc
     8581 gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg
     8641 cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct
     8701 aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc
     8761 gcacccgtgg ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg
     8821 cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca
     8881 ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg
     8941 ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag
     9001 tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc
     9061 gagatcccgg tgcctaatga gtgagctaac ttacattaat tgcgttgcgc tcactgcccg
     9121 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
     9181 gaggcggttt gcgtattggg cgccagggtg gtttttcttt tcaccagtga gacgggcaac
     9241 agctgattgc ccttcaccgc ctggccctga gagagttgca gcaagcggtc cacgctggtt
     9301 tgccccagca ggcgaaaatc ctgtttgatg gtggttaacg gcgggatata acatgagctg
     9361 tcttcggtat cgtcgtatcc cactaccgag atatccgcac caacgcgcag cccggactcg
     9421 gtaatggcgc gcattgcgcc cagcgccatc tgatcgttgg caaccagcat cgcagtggga
     9481 acgatgccct cattc
//
