LOCUS       Exported                9495 bp ds-DNA     circular SYN 21-OCT-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9495)
  AUTHORS   locusliu
  TITLE     Direct Submission
  JOURNAL   Exported Monday, May 16, 2022 from SnapGene Viewer 5.1.7
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9495
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        complement(699..776)
                     /gene="lacI"
                     /label=lacI promoter
     promoter        1085..1103
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    1104..1128
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             1143..1165
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             1173..5093
                     /codon_start=1
                     /product="CRISPR-associated protein from Acidaminococcus 
                     sp. BV3L6 (Zetsche et al., 2015)"
                     /label=AsCpf1
                     /translation="MTQFEGFTNLYQVSKTLRFELIPQGKTLKHIQEQGFIEEDKARND
                     HYKELKPIIDRIYKTYADQCLQLVQLDWENLSAAIDSYRKEKTEETRNALIEEQATYRN
                     AIHDYFIGRTDNLTDAINKRHAEIYKGLFKAELFNGKVLKQLGTVTTTEHENALLRSFD
                     KFTTYFSGFYENRKNVFSAEDISTAIPHRIVQDNFPKFKENCHIFTRLITAVPSLREHF
                     ENVKKAIGIFVSTSIEEVFSFPFYNQLLTQTQIDLYNQLLGGISREAGTEKIKGLNEVL
                     NLAIQKNDETAHIIASLPHRFIPLFKQILSDRNTLSFILEEFKSDEEVIQSFCKYKTLL
                     RNENVLETAEALFNELNSIDLTHIFISHKKLETISSALCDHWDTLRNALYERRISELTG
                     KITKSAKEKVQRSLKHEDINLQEIISAAGKELSEAFKQKTSEILSHAHAALDQPLPTTL
                     KKQEEKEILKSQLDSLLGLYHLLDWFAVDESNEVDPEFSARLTGIKLEMEPSLSFYNKA
                     RNYATKKPYSVEKFKLNFQMPTLASGWDVNKEKNNGAILFVKNGLYYLGIMPKQKGRYK
                     ALSFEPTEKTSEGFDKMYYDYFPDAAKMIPKCSTQLKAVTAHFQTHTTPILLSNNFIEP
                     LEITKEIYDLNNPEKEPKKFQTAYAKKTGDQKGYREALCKWIDFTRDFLSKYTKTTSID
                     LSSLRPSSQYKDLGEYYAELNPLLYHISFQRIAEKEIMDAVETGKLYLFQIYNKDFAKG
                     HHGKPNLHTLYWTGLFSPENLAKTSIKLNGQAELFYRPKSRMKRMAHRLGEKMLNKKLK
                     DQKTPIPDTLYQELYDYVNHRLSHDLSDEARALLPNVITKEVSHEIIKDRRFTSDKFFF
                     HVPITLNYQAANSPSKFNQRVNAYLKEHPETPIIGIDRGERNLIYITVIDSTGKILEQR
                     SLNTIQQFDYQKKLDNREKERVAARQAWSVVGTIKDLKQGYLSQVIHEIVDLMIHYQAV
                     VVLENLNFGFKSKRTGIAEKAVYQQFEKMLIDKLNCLVLKDYPAEKVGGVLNPYQLTDQ
                     FTSFAKMGTQSGFLFYVPAPYTSKIDPLTGFVDPFVWKTIKNHESRKHFLEGFDFLHYD
                     VKTGDFILHFKMNRNLSFQRGLPGFMPAWDIVFEKNETQFDAKGTPFIAGKRIVPVIEN
                     HRFTGRYRDLYPANELIALLEEKGIVFRDGSNILPKLLENDDSHAIDTMVALIRSVLQM
                     RNSNAATGEDYINSPVRDLNGVCFDSRFQNPEWPMDADANGAYHIALKGQLLLNHLKES
                     KDLKLQNGISNQDWLAYIQELRN"
     regulatory      4813..4822
                     /regulatory_class="other"
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             5094..5141
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             5148..5228
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
     CDS             5229..5255
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     CDS             5274..5300
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     CDS             5307..5324
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      5391..5438
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      5475..5930
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        5957..6061
                     /gene="bla"
                     /label=AmpR promoter
     CDS             6062..6922
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      7093..7681
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    7867..8009
                     /label=bom
                     /note="basis of mobility region from pBR322"
     CDS             complement(8111..8302)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(join(9111..9495,1..698))
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
ORIGIN
        1 agcatttgca tggtttgttg aaaaccggac atggcactcc agtcgccttc ccgttccgct
       61 atcggctgaa tttgattgcg agtgagatat ttatgccagc cagccagacg cagacgcgcc
      121 gagacagaac ttaatgggcc cgctaacagc gcgatttgct ggtgacccaa tgcgaccaga
      181 tgctccacgc ccagtcgcgt accgtcttca tgggagaaaa taatactgtt gatgggtgtc
      241 tggtcagaga catcaagaaa taacgccgga acattagtgc aggcagcttc cacagcaatg
      301 gcatcctggt catccagcgg atagttaatg atcagcccac tgacgcgttg cgcgagaaga
      361 ttgtgcaccg ccgctttaca ggcttcgacg ccgcttcgtt ctaccatcga caccaccacg
      421 ctggcaccca gttgatcggc gcgagattta atcgccgcga caatttgcga cggcgcgtgc
      481 agggccagac tggaggtggc aacgccaatc agcaacgact gtttgcccgc cagttgttgt
      541 gccacgcggt tgggaatgta attcagctcc gccatcgccg cttccacttt ttcccgcgtt
      601 ttcgcagaaa cgtggctggc ctggttcacc acgcgggaaa cggtctgata agagacaccg
      661 gcatactctg cgacatcgta taacgttact ggtttcacat tcaccaccct gaattgactc
      721 tcttccgggc gctatcatgc cataccgcga aaggttttgc gccattcgat ggtgtccggg
      781 atctcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag
      841 gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc
      901 cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg
      961 gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt
     1021 ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg
     1081 aaattaatac gactcactat aggggaattg tgagcggata acaattcccc tctagaaata
     1141 attttgttta actttaagaa ggagatatac atatgacaca gttcgagggc tttaccaacc
     1201 tgtatcaggt gagcaagaca ctgcggtttg agctgatccc acagggcaag accctgaagc
     1261 acatccagga gcagggcttc atcgaggagg acaaggcccg caatgatcac tacaaggagc
     1321 tgaagcccat catcgatcgg atctacaaga cctatgccga ccagtgcctg cagctggtgc
     1381 agctggattg ggagaacctg agcgccgcca tcgactccta tagaaaggag aaaaccgagg
     1441 agacaaggaa cgccctgatc gaggagcagg ccacatatcg caatgccatc cacgactact
     1501 tcatcggccg gacagacaac ctgaccgatg ccatcaataa gagacacgcc gagatctaca
     1561 agggcctgtt caaggccgag ctgtttaatg gcaaggtgct gaagcagctg ggcaccgtga
     1621 ccacaaccga gcacgagaac gccctgctgc ggagcttcga caagtttaca acctacttct
     1681 ccggctttta tgagaacagg aagaacgtgt tcagcgccga ggatatcagc acagccatcc
     1741 cacaccgcat cgtgcaggac aacttcccca agtttaagga gaattgtcac atcttcacac
     1801 gcctgatcac cgccgtgccc agcctgcggg agcactttga gaacgtgaag aaggccatcg
     1861 gcatcttcgt gagcacctcc atcgaggagg tgttttcctt ccctttttat aaccagctgc
     1921 tgacacagac ccagatcgac ctgtataacc agctgctggg aggaatctct cgggaggcag
     1981 gcaccgagaa gatcaagggc ctgaacgagg tgctgaatct ggccatccag aagaatgatg
     2041 agacagccca catcatcgcc tccctgccac acagattcat ccccctgttt aagcagatcc
     2101 tgtccgatag gaacaccctg tctttcatcc tggaggagtt taagagcgac gaggaagtga
     2161 tccagtcctt ctgcaagtac aagacactgc tgagaaacga gaacgtgctg gagacagccg
     2221 aggccctgtt taacgagctg aacagcatcg acctgacaca catcttcatc agccacaaga
     2281 agctggagac aatcagcagc gccctgtgcg accactggga tacactgagg aatgccctgt
     2341 atgagcggag aatctccgag ctgacaggca agatcaccaa gtctgccaag gagaaggtgc
     2401 agcgcagcct gaagcacgag gatatcaacc tgcaggagat catctctgcc gcaggcaagg
     2461 agctgagcga ggccttcaag cagaaaacca gcgagatcct gtcccacgca cacgccgccc
     2521 tggatcagcc actgcctaca accctgaaga agcaggagga gaaggagatc ctgaagtctc
     2581 agctggacag cctgctgggc ctgtaccacc tgctggactg gtttgccgtg gatgagtcca
     2641 acgaggtgga ccccgagttc tctgcccggc tgaccggcat caagctggag atggagcctt
     2701 ctctgagctt ctacaacaag gccagaaatt atgccaccaa gaagccctac tccgtggaga
     2761 agttcaagct gaactttcag atgcctacac tggcctctgg ctgggacgtg aataaggaga
     2821 agaacaatgg cgccatcctg tttgtgaaga acggcctgta ctatctgggc atcatgccaa
     2881 agcagaaggg caggtataag gccctgagct tcgagcccac agagaaaacc agcgagggct
     2941 ttgataagat gtactatgac tacttccctg atgccgccaa gatgatccca aagtgcagca
     3001 cccagctgaa ggccgtgaca gcccactttc agacccacac aacccccatc ctgctgtcca
     3061 acaatttcat cgagcctctg gagatcacaa aggagatcta cgacctgaac aatcctgaga
     3121 aggagccaaa gaagtttcag acagcctacg ccaagaaaac cggcgaccag aagggctaca
     3181 gagaggccct gtgcaagtgg atcgacttca caagggattt tctgtccaag tataccaaga
     3241 caacctctat cgatctgtct agcctgcggc catcctctca gtataaggac ctgggcgagt
     3301 actatgccga gctgaatccc ctgctgtacc acatcagctt ccagagaatc gccgagaagg
     3361 agatcatgga tgccgtggag acaggcaagc tgtacctgtt ccagatctat aacaaggact
     3421 ttgccaaggg ccaccacggc aagcctaatc tgcacacact gtattggacc ggcctgtttt
     3481 ctccagagaa cctggccaag acaagcatca agctgaatgg ccaggccgag ctgttctacc
     3541 gccctaagtc caggatgaag aggatggcac accggctggg agagaagatg ctgaacaaga
     3601 agctgaagga tcagaaaacc ccaatccccg acaccctgta ccaggagctg tacgactatg
     3661 tgaatcacag actgtcccac gacctgtctg atgaggccag ggccctgctg cccaacgtga
     3721 tcaccaagga ggtgtctcac gagatcatca aggataggcg ctttaccagc gacaagttct
     3781 ttttccacgt gcctatcaca ctgaactatc aggccgccaa ttccccatct aagttcaacc
     3841 agagggtgaa tgcctacctg aaggagcacc ccgagacacc tatcatcggc atcgatcggg
     3901 gcgagagaaa cctgatctat atcacagtga tcgactccac cggcaagatc ctggagcagc
     3961 ggagcctgaa caccatccag cagtttgatt accagaagaa gctggacaac agggagaagg
     4021 agagggtggc agcaaggcag gcctggtctg tggtgggcac aatcaaggat ctgaagcagg
     4081 gctatctgag ccaggtcatc cacgagatcg tggacctgat gatccactac caggccgtgg
     4141 tggtgctgga gaacctgaat ttcggcttta agagcaagag gaccggcatc gccgagaagg
     4201 ccgtgtacca gcagttcgag aagatgctga tcgataagct gaattgcctg gtgctgaagg
     4261 actatccagc agagaaagtg ggaggcgtgc tgaacccata ccagctgaca gaccagttca
     4321 cctcctttgc caagatgggc acccagtctg gcttcctgtt ttacgtgcct gccccatata
     4381 catctaagat cgatcccctg accggcttcg tggacccctt cgtgtggaaa accatcaaga
     4441 atcacgagag ccgcaagcac ttcctggagg gcttcgactt tctgcactac gacgtgaaaa
     4501 ccggcgactt catcctgcac tttaagatga acagaaatct gtccttccag aggggcctgc
     4561 ccggctttat gcctgcatgg gatatcgtgt tcgagaagaa cgagacacag tttgacgcca
     4621 agggcacccc tttcatcgcc ggcaagagaa tcgtgccagt gatcgagaat cacagattca
     4681 ccggcagata ccgggacctg tatcctgcca acgagctgat cgccctgctg gaggagaagg
     4741 gcatcgtgtt cagggatggc tccaacatcc tgccaaagct gctggagaat gacgattctc
     4801 acgccatcga caccatggtg gccctgatcc gcagcgtgct gcagatgcgg aactccaatg
     4861 ccgccacagg cgaggactat atcaacagcc ccgtgcgcga tctgaatggc gtgtgcttcg
     4921 actcccggtt tcagaaccca gagtggccca tggacgccga tgccaatggc gcctaccaca
     4981 tcgccctgaa gggccagctg ctgctgaatc acctgaagga gagcaaggat ctgaagctgc
     5041 agaacggcat ctccaatcag gactggctgg cctacatcca ggagctgcgc aacaaaaggc
     5101 cggcggccac gaaaaaggcc ggccaggcaa aaaagaaaaa gggatcctac ccatacgatg
     5161 ttccagatta cgcttatccc tacgacgtgc ctgattatgc atacccatat gatgtccccg
     5221 actatgcccc ggcagctaag agggtgaaac tggatggcgg tagcggcgga ggccccgccg
     5281 ccaagcgcgt gaagctggac ctcgagcacc accaccacca ccactgagat ccggctgcta
     5341 acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac
     5401 cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg
     5461 gattggcgaa tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac
     5521 gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc
     5581 ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt
     5641 agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg
     5701 ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac
     5761 gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta
     5821 ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat
     5881 ttaacaaaaa tttaacgcga attttaacaa aatattaacg tttacaattt caggtggcac
     5941 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
     6001 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
     6061 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
     6121 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
     6181 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
     6241 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
     6301 ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
     6361 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
     6421 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
     6481 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
     6541 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
     6601 gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
     6661 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
     6721 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
     6781 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
     6841 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
     6901 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
     6961 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
     7021 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     7081 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     7141 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     7201 ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt
     7261 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     7321 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     7381 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     7441 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     7501 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     7561 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     7621 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     7681 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     7741 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
     7801 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
     7861 agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata
     7921 tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc
     7981 tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc
     8041 cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga
     8101 gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa
     8161 gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct
     8221 cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg
     8281 cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg
     8341 taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg
     8401 cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga
     8461 gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg
     8521 gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc
     8581 gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg
     8641 cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct
     8701 aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc
     8761 gcacccgtgg ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg
     8821 cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca
     8881 ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg
     8941 ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag
     9001 tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc
     9061 gagatcccgg tgcctaatga gtgagctaac ttacattaat tgcgttgcgc tcactgcccg
     9121 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
     9181 gaggcggttt gcgtattggg cgccagggtg gtttttcttt tcaccagtga gacgggcaac
     9241 agctgattgc ccttcaccgc ctggccctga gagagttgca gcaagcggtc cacgctggtt
     9301 tgccccagca ggcgaaaatc ctgtttgatg gtggttaacg gcgggatata acatgagctg
     9361 tcttcggtat cgtcgtatcc cactaccgag atatccgcac caacgcgcag cccggactcg
     9421 gtaatggcgc gcattgcgcc cagcgccatc tgatcgttgg caaccagcat cgcagtggga
     9481 acgatgccct cattc
//
