LOCUS       Exported                9213 bp ds-DNA     circular SYN 19-APR-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9213)
  AUTHORS   locusliu
  TITLE     Direct Submission
  JOURNAL   Exported Monday, May 16, 2022 from SnapGene Viewer 5.1.7
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9213
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        complement(699..776)
                     /gene="lacI"
                     /label=lacI promoter
     promoter        1085..1103
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    1104..1128
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             1143..1165
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             1173..4856
                     /codon_start=1
                     /product="CRISPR-associated protein from Lachnospiraceae 
                     bacterium ND2006 (Zetsche et al., 2015)"
                     /label=LbCpf1
                     /translation="MSKLEKFTNCYSLSKTLRFKAIPVGKTQENIDNKRLLVEDEKRAE
                     DYKGVKKLLDRYYLSFINDVLHSIKLKNLNNYISLFRKKTRTEKENKELENLEINLRKE
                     IAKAFKGNEGYKSLFKKDIIETILPEFLDDKDEIALVNSFNGFTTAFTGFFDNRENMFS
                     EEAKSTSIAFRCINENLTRYISNMDIFEKVDAIFDKHEVQEIKEKILNSDYDVEDFFEG
                     EFFNFVLTQEGIDVYNAIIGGFVTESGEKIKGLNEYINLYNQKTKQKLPKFKPLYKQVL
                     SDRESLSFYGEGYTSDEEVLEVFRNTLNKNSEIFSSIKKLEKLFKNFDEYSSAGIFVKN
                     GPAISTISKDIFGEWNVIRDKWNAEYDDIHLKKKAVVTEKYEDDRRKSFKKIGSFSLEQ
                     LQEYADADLSVVEKLKEIIIQKVDEIYKVYGSSEKLFDADFVLEKSLKKNDAVVAIMKD
                     LLDSVKSFENYIKAFFGEGKETNRDESFYGDFVLAYDILLKVDHIYDAIRNYVTQKPYS
                     KDKFKLYFQNPQFMGGWDKDKETDYRATILRYGSKYYLAIMDKKYAKCLQKIDKDDVNG
                     NYEKINYKLLPGPNKMLPKVFFSKKWMAYYNPSEDIQKIYKNGTFKKGDMFNLNDCHKL
                     IDFFKDSISRYPKWSNAYDFNFSETEKYKDIAGFYREVEEQGYKVSFESASKKEVDKLV
                     EEGKLYMFQIYNKDFSDKSHGTPNLHTMYFKLLFDENNHGQIRLSGGAELFMRRASLKK
                     EELVVHPANSPIANKNPDNPKKTTTLSYDVYKDKRFSEDQYELHIPIAINKCPKNIFKI
                     NTEVRVLLKHDDNPYVIGIDRGERNLLYIVVVDGKGNIVEQYSLNEIINNFNGIRIKTD
                     YHSLLDKKEKERFEARQNWTSIENIKELKAGYISQVVHKICELVEKYDAVIALEDLNSG
                     FKNSRVKVEKQVYQKFEKMLIDKLNYMVDKKSNPCATGGALKGYQITNKFESFKSMSTQ
                     NGFIFYIPAWLTSKIDPSTGFVNLLKTKYTSIADSKKFISSFDRIMYVPEEDLFEFALD
                     YKNFSRTDADYIKKWKLYSYGNRIRIFRNPKKNNVFDWEEVCLTSAYKELFNKYGINYQ
                     QGDIRALLCEQSDKAFYSSFMALMSLMLQMRNSITGRTDVDFLISPVKNSDGIFYDSRN
                     YEAQENAILPKNADANGAYNIARKVLWAIGQFKKAEDEKLDKVKIAISNKEWLEYAQTS
                     VKH"
     CDS             4857..4904
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             4911..4991
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
     CDS             4992..5018
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     CDS             5025..5042
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      5109..5156
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      5193..5648
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        5675..5779
                     /gene="bla"
                     /label=AmpR promoter
     CDS             5780..6640
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      6811..7399
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    7585..7727
                     /label=bom
                     /note="basis of mobility region from pBR322"
     CDS             complement(7829..8020)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(join(8829..9213,1..698))
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
ORIGIN
        1 agcatttgca tggtttgttg aaaaccggac atggcactcc agtcgccttc ccgttccgct
       61 atcggctgaa tttgattgcg agtgagatat ttatgccagc cagccagacg cagacgcgcc
      121 gagacagaac ttaatgggcc cgctaacagc gcgatttgct ggtgacccaa tgcgaccaga
      181 tgctccacgc ccagtcgcgt accgtcttca tgggagaaaa taatactgtt gatgggtgtc
      241 tggtcagaga catcaagaaa taacgccgga acattagtgc aggcagcttc cacagcaatg
      301 gcatcctggt catccagcgg atagttaatg atcagcccac tgacgcgttg cgcgagaaga
      361 ttgtgcaccg ccgctttaca ggcttcgacg ccgcttcgtt ctaccatcga caccaccacg
      421 ctggcaccca gttgatcggc gcgagattta atcgccgcga caatttgcga cggcgcgtgc
      481 agggccagac tggaggtggc aacgccaatc agcaacgact gtttgcccgc cagttgttgt
      541 gccacgcggt tgggaatgta attcagctcc gccatcgccg cttccacttt ttcccgcgtt
      601 ttcgcagaaa cgtggctggc ctggttcacc acgcgggaaa cggtctgata agagacaccg
      661 gcatactctg cgacatcgta taacgttact ggtttcacat tcaccaccct gaattgactc
      721 tcttccgggc gctatcatgc cataccgcga aaggttttgc gccattcgat ggtgtccggg
      781 atctcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag
      841 gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc
      901 cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg
      961 gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt
     1021 ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg
     1081 aaattaatac gactcactat aggggaattg tgagcggata acaattcccc tctagaaata
     1141 attttgttta actttaagaa ggagatatac atatgagcaa gctggagaag tttacaaact
     1201 gctactccct gtctaagacc ctgaggttca aggccatccc tgtgggcaag acccaggaga
     1261 acatcgacaa taagcggctg ctggtggagg acgagaagag agccgaggat tataagggcg
     1321 tgaagaagct gctggatcgc tactatctgt cttttatcaa cgacgtgctg cacagcatca
     1381 agctgaagaa tctgaacaat tacatcagcc tgttccggaa gaaaaccaga accgagaagg
     1441 agaataagga gctggagaac ctggagatca atctgcggaa ggagatcgcc aaggccttca
     1501 agggcaacga gggctacaag tccctgttta agaaggatat catcgagaca atcctgccag
     1561 agttcctgga cgataaggac gagatcgccc tggtgaacag cttcaatggc tttaccacag
     1621 ccttcaccgg cttctttgat aacagagaga atatgttttc cgaggaggcc aagagcacat
     1681 ccatcgcctt caggtgtatc aacgagaatc tgacccgcta catctctaat atggacatct
     1741 tcgagaaggt ggacgccatc tttgataagc acgaggtgca ggagatcaag gagaagatcc
     1801 tgaacagcga ctatgatgtg gaggatttct ttgagggcga gttctttaac tttgtgctga
     1861 cacaggaggg catcgacgtg tataacgcca tcatcggcgg cttcgtgacc gagagcggcg
     1921 agaagatcaa gggcctgaac gagtacatca acctgtataa tcagaaaacc aagcagaagc
     1981 tgcctaagtt taagccactg tataagcagg tgctgagcga tcgggagtct ctgagcttct
     2041 acggcgaggg ctatacatcc gatgaggagg tgctggaggt gtttagaaac accctgaaca
     2101 agaacagcga gatcttcagc tccatcaaga agctggagaa gctgttcaag aattttgacg
     2161 agtactctag cgccggcatc tttgtgaaga acggccccgc catcagcaca atctccaagg
     2221 atatcttcgg cgagtggaac gtgatccggg acaagtggaa tgccgagtat gacgatatcc
     2281 acctgaagaa gaaggccgtg gtgaccgaga agtacgagga cgatcggaga aagtccttca
     2341 agaagatcgg ctccttttct ctggagcagc tgcaggagta cgccgacgcc gatctgtctg
     2401 tggtggagaa gctgaaggag atcatcatcc agaaggtgga tgagatctac aaggtgtatg
     2461 gctcctctga gaagctgttc gacgccgatt ttgtgctgga gaagagcctg aagaagaacg
     2521 acgccgtggt ggccatcatg aaggacctgc tggattctgt gaagagcttc gagaattaca
     2581 tcaaggcctt ctttggcgag ggcaaggaga caaacaggga cgagtccttc tatggcgatt
     2641 ttgtgctggc ctacgacatc ctgctgaagg tggaccacat ctacgatgcc atccgcaatt
     2701 atgtgaccca gaagccctac tctaaggata agttcaagct gtattttcag aaccctcagt
     2761 tcatgggcgg ctgggacaag gataaggaga cagactatcg ggccaccatc ctgagatacg
     2821 gctccaagta ctatctggcc atcatggata agaagtacgc caagtgcctg cagaagatcg
     2881 acaaggacga tgtgaacggc aattacgaga agatcaacta taagctgctg cccggcccta
     2941 ataagatgct gccaaaggtg ttcttttcta agaagtggat ggcctactat aaccccagcg
     3001 aggacatcca gaagatctac aagaatggca cattcaagaa gggcgatatg tttaacctga
     3061 atgactgtca caagctgatc gacttcttta aggatagcat ctcccggtat ccaaagtggt
     3121 ccaatgccta cgatttcaac ttttctgaga cagagaagta taaggacatc gccggctttt
     3181 acagagaggt ggaggagcag ggctataagg tgagcttcga gtctgccagc aagaaggagg
     3241 tggataagct ggtggaggag ggcaagctgt atatgttcca gatctataac aaggactttt
     3301 ccgataagtc tcacggcaca cccaatctgc acaccatgta cttcaagctg ctgtttgacg
     3361 agaacaatca cggacagatc aggctgagcg gaggagcaga gctgttcatg aggcgcgcct
     3421 ccctgaagaa ggaggagctg gtggtgcacc cagccaactc ccctatcgcc aacaagaatc
     3481 cagataatcc caagaaaacc acaaccctgt cctacgacgt gtataaggat aagaggtttt
     3541 ctgaggacca gtacgagctg cacatcccaa tcgccatcaa taagtgcccc aagaacatct
     3601 tcaagatcaa tacagaggtg cgcgtgctgc tgaagcacga cgataacccc tatgtgatcg
     3661 gcatcgatag gggcgagcgc aatctgctgt atatcgtggt ggtggacggc aagggcaaca
     3721 tcgtggagca gtattccctg aacgagatca tcaacaactt caacggcatc aggatcaaga
     3781 cagattacca ctctctgctg gacaagaagg agaaggagag gttcgaggcc cgccagaact
     3841 ggacctccat cgagaatatc aaggagctga aggccggcta tatctctcag gtggtgcaca
     3901 agatctgcga gctggtggag aagtacgatg ccgtgatcgc cctggaggac ctgaactctg
     3961 gctttaagaa tagccgcgtg aaggtggaga agcaggtgta tcagaagttc gagaagatgc
     4021 tgatcgataa gctgaactac atggtggaca agaagtctaa tccttgtgca acaggcggcg
     4081 ccctgaaggg ctatcagatc accaataagt tcgagagctt taagtccatg tctacccaga
     4141 acggcttcat cttttacatc cctgcctggc tgacatccaa gatcgatcca tctaccggct
     4201 ttgtgaacct gctgaaaacc aagtatacca gcatcgccga ttccaagaag ttcatcagct
     4261 cctttgacag gatcatgtac gtgcccgagg aggatctgtt cgagtttgcc ctggactata
     4321 agaacttctc tcgcacagac gccgattaca tcaagaagtg gaagctgtac tcctacggca
     4381 accggatcag aatcttccgg aatcctaaga agaacaacgt gttcgactgg gaggaggtgt
     4441 gcctgaccag cgcctataag gagctgttca acaagtacgg catcaattat cagcagggcg
     4501 atatcagagc cctgctgtgc gagcagtccg acaaggcctt ctactctagc tttatggccc
     4561 tgatgagcct gatgctgcag atgcggaaca gcatcacagg ccgcaccgac gtggattttc
     4621 tgatcagccc tgtgaagaac tccgacggca tcttctacga tagccggaac tatgaggccc
     4681 aggagaatgc catcctgcca aagaacgccg acgccaatgg cgcctataac atcgccagaa
     4741 aggtgctgtg ggccatcggc cagttcaaga aggccgagga cgagaagctg gataaggtga
     4801 agatcgccat ctctaacaag gagtggctgg agtacgccca gaccagcgtg aagcacaaaa
     4861 ggccggcggc cacgaaaaag gccggccagg caaaaaagaa aaagggatcc tacccatacg
     4921 atgttccaga ttacgcttat ccctacgacg tgcctgatta tgcataccca tatgatgtcc
     4981 ccgactatgc cccggcagct aagagggtga aactggatct cgagcaccac caccaccacc
     5041 actgagatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg
     5101 agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga
     5161 aaggaggaac tatatccgga ttggcgaatg ggacgcgccc tgtagcggcg cattaagcgc
     5221 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc
     5281 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
     5341 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
     5401 acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
     5461 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact
     5521 caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg
     5581 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt
     5641 tacaatttca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
     5701 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
     5761 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
     5821 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
     5881 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
     5941 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
     6001 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca
     6061 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
     6121 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
     6181 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
     6241 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
     6301 cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg
     6361 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
     6421 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
     6481 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
     6541 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
     6601 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
     6661 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
     6721 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     6781 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     6841 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     6901 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct
     6961 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     7021 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     7081 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     7141 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     7201 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     7261 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     7321 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     7381 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     7441 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
     7501 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
     7561 agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg
     7621 tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta
     7681 agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc
     7741 caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag
     7801 ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg
     7861 cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct
     7921 gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa
     7981 agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg
     8041 gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg
     8101 ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat
     8161 ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag
     8221 atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg
     8281 tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc
     8341 atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta
     8401 tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg
     8461 acaggagcac gatcatgcgc acccgtgggg ccgccatgcc ggcgataatg gcctgcttct
     8521 cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc
     8581 cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga
     8641 aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca
     8701 taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg
     8761 ctctcaaggg catcggtcga gatcccggtg cctaatgagt gagctaactt acattaattg
     8821 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
     8881 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ccagggtggt ttttcttttc
     8941 accagtgaga cgggcaacag ctgattgccc ttcaccgcct ggccctgaga gagttgcagc
     9001 aagcggtcca cgctggtttg ccccagcagg cgaaaatcct gtttgatggt ggttaacggc
     9061 gggatataac atgagctgtc ttcggtatcg tcgtatccca ctaccgagat atccgcacca
     9121 acgcgcagcc cggactcggt aatggcgcgc attgcgccca gcgccatctg atcgttggca
     9181 accagcatcg cagtgggaac gatgccctca ttc
//
