LOCUS       Exported                9258 bp ds-DNA     circular SYN 21-OCT-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9258)
  AUTHORS   locusliu
  TITLE     Direct Submission
  JOURNAL   Exported Monday, May 16, 2022 from SnapGene Viewer 5.1.7
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9258
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        complement(699..776)
                     /gene="lacI"
                     /label=lacI promoter
     promoter        1085..1103
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    1104..1128
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             1143..1165
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             1173..4856
                     /codon_start=1
                     /product="CRISPR-associated protein from Lachnospiraceae 
                     bacterium ND2006 (Zetsche et al., 2015)"
                     /label=LbCpf1
                     /translation="MSKLEKFTNCYSLSKTLRFKAIPVGKTQENIDNKRLLVEDEKRAE
                     DYKGVKKLLDRYYLSFINDVLHSIKLKNLNNYISLFRKKTRTEKENKELENLEINLRKE
                     IAKAFKGNEGYKSLFKKDIIETILPEFLDDKDEIALVNSFNGFTTAFTGFFDNRENMFS
                     EEAKSTSIAFRCINENLTRYISNMDIFEKVDAIFDKHEVQEIKEKILNSDYDVEDFFEG
                     EFFNFVLTQEGIDVYNAIIGGFVTESGEKIKGLNEYINLYNQKTKQKLPKFKPLYKQVL
                     SDRESLSFYGEGYTSDEEVLEVFRNTLNKNSEIFSSIKKLEKLFKNFDEYSSAGIFVKN
                     GPAISTISKDIFGEWNVIRDKWNAEYDDIHLKKKAVVTEKYEDDRRKSFKKIGSFSLEQ
                     LQEYADADLSVVEKLKEIIIQKVDEIYKVYGSSEKLFDADFVLEKSLKKNDAVVAIMKD
                     LLDSVKSFENYIKAFFGEGKETNRDESFYGDFVLAYDILLKVDHIYDAIRNYVTQKPYS
                     KDKFKLYFQNPQFMGGWDKDKETDYRATILRYGSKYYLAIMDKKYAKCLQKIDKDDVNG
                     NYEKINYKLLPGPNKMLPKVFFSKKWMAYYNPSEDIQKIYKNGTFKKGDMFNLNDCHKL
                     IDFFKDSISRYPKWSNAYDFNFSETEKYKDIAGFYREVEEQGYKVSFESASKKEVDKLV
                     EEGKLYMFQIYNKDFSDKSHGTPNLHTMYFKLLFDENNHGQIRLSGGAELFMRRASLKK
                     EELVVHPANSPIANKNPDNPKKTTTLSYDVYKDKRFSEDQYELHIPIAINKCPKNIFKI
                     NTEVRVLLKHDDNPYVIGIDRGERNLLYIVVVDGKGNIVEQYSLNEIINNFNGIRIKTD
                     YHSLLDKKEKERFEARQNWTSIENIKELKAGYISQVVHKICELVEKYDAVIALEDLNSG
                     FKNSRVKVEKQVYQKFEKMLIDKLNYMVDKKSNPCATGGALKGYQITNKFESFKSMSTQ
                     NGFIFYIPAWLTSKIDPSTGFVNLLKTKYTSIADSKKFISSFDRIMYVPEEDLFEFALD
                     YKNFSRTDADYIKKWKLYSYGNRIRIFRNPKKNNVFDWEEVCLTSAYKELFNKYGINYQ
                     QGDIRALLCEQSDKAFYSSFMALMSLMLQMRNSITGRTDVDFLISPVKNSDGIFYDSRN
                     YEAQENAILPKNADANGAYNIARKVLWAIGQFKKAEDEKLDKVKIAISNKEWLEYAQTS
                     VKH"
     CDS             4857..4904
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             4911..4991
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
     CDS             4992..5018
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     CDS             5037..5063
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     CDS             5070..5087
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      5154..5201
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      5238..5693
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        5720..5824
                     /gene="bla"
                     /label=AmpR promoter
     CDS             5825..6685
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      6856..7444
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    7630..7772
                     /label=bom
                     /note="basis of mobility region from pBR322"
     CDS             complement(7874..8065)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(join(8874..9258,1..698))
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
ORIGIN
        1 agcatttgca tggtttgttg aaaaccggac atggcactcc agtcgccttc ccgttccgct
       61 atcggctgaa tttgattgcg agtgagatat ttatgccagc cagccagacg cagacgcgcc
      121 gagacagaac ttaatgggcc cgctaacagc gcgatttgct ggtgacccaa tgcgaccaga
      181 tgctccacgc ccagtcgcgt accgtcttca tgggagaaaa taatactgtt gatgggtgtc
      241 tggtcagaga catcaagaaa taacgccgga acattagtgc aggcagcttc cacagcaatg
      301 gcatcctggt catccagcgg atagttaatg atcagcccac tgacgcgttg cgcgagaaga
      361 ttgtgcaccg ccgctttaca ggcttcgacg ccgcttcgtt ctaccatcga caccaccacg
      421 ctggcaccca gttgatcggc gcgagattta atcgccgcga caatttgcga cggcgcgtgc
      481 agggccagac tggaggtggc aacgccaatc agcaacgact gtttgcccgc cagttgttgt
      541 gccacgcggt tgggaatgta attcagctcc gccatcgccg cttccacttt ttcccgcgtt
      601 ttcgcagaaa cgtggctggc ctggttcacc acgcgggaaa cggtctgata agagacaccg
      661 gcatactctg cgacatcgta taacgttact ggtttcacat tcaccaccct gaattgactc
      721 tcttccgggc gctatcatgc cataccgcga aaggttttgc gccattcgat ggtgtccggg
      781 atctcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag
      841 gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc
      901 cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg
      961 gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt
     1021 ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg
     1081 aaattaatac gactcactat aggggaattg tgagcggata acaattcccc tctagaaata
     1141 attttgttta actttaagaa ggagatatac atatgagcaa gctggagaag tttacaaact
     1201 gctactccct gtctaagacc ctgaggttca aggccatccc tgtgggcaag acccaggaga
     1261 acatcgacaa taagcggctg ctggtggagg acgagaagag agccgaggat tataagggcg
     1321 tgaagaagct gctggatcgc tactatctgt cttttatcaa cgacgtgctg cacagcatca
     1381 agctgaagaa tctgaacaat tacatcagcc tgttccggaa gaaaaccaga accgagaagg
     1441 agaataagga gctggagaac ctggagatca atctgcggaa ggagatcgcc aaggccttca
     1501 agggcaacga gggctacaag tccctgttta agaaggatat catcgagaca atcctgccag
     1561 agttcctgga cgataaggac gagatcgccc tggtgaacag cttcaatggc tttaccacag
     1621 ccttcaccgg cttctttgat aacagagaga atatgttttc cgaggaggcc aagagcacat
     1681 ccatcgcctt caggtgtatc aacgagaatc tgacccgcta catctctaat atggacatct
     1741 tcgagaaggt ggacgccatc tttgataagc acgaggtgca ggagatcaag gagaagatcc
     1801 tgaacagcga ctatgatgtg gaggatttct ttgagggcga gttctttaac tttgtgctga
     1861 cacaggaggg catcgacgtg tataacgcca tcatcggcgg cttcgtgacc gagagcggcg
     1921 agaagatcaa gggcctgaac gagtacatca acctgtataa tcagaaaacc aagcagaagc
     1981 tgcctaagtt taagccactg tataagcagg tgctgagcga tcgggagtct ctgagcttct
     2041 acggcgaggg ctatacatcc gatgaggagg tgctggaggt gtttagaaac accctgaaca
     2101 agaacagcga gatcttcagc tccatcaaga agctggagaa gctgttcaag aattttgacg
     2161 agtactctag cgccggcatc tttgtgaaga acggccccgc catcagcaca atctccaagg
     2221 atatcttcgg cgagtggaac gtgatccggg acaagtggaa tgccgagtat gacgatatcc
     2281 acctgaagaa gaaggccgtg gtgaccgaga agtacgagga cgatcggaga aagtccttca
     2341 agaagatcgg ctccttttct ctggagcagc tgcaggagta cgccgacgcc gatctgtctg
     2401 tggtggagaa gctgaaggag atcatcatcc agaaggtgga tgagatctac aaggtgtatg
     2461 gctcctctga gaagctgttc gacgccgatt ttgtgctgga gaagagcctg aagaagaacg
     2521 acgccgtggt ggccatcatg aaggacctgc tggattctgt gaagagcttc gagaattaca
     2581 tcaaggcctt ctttggcgag ggcaaggaga caaacaggga cgagtccttc tatggcgatt
     2641 ttgtgctggc ctacgacatc ctgctgaagg tggaccacat ctacgatgcc atccgcaatt
     2701 atgtgaccca gaagccctac tctaaggata agttcaagct gtattttcag aaccctcagt
     2761 tcatgggcgg ctgggacaag gataaggaga cagactatcg ggccaccatc ctgagatacg
     2821 gctccaagta ctatctggcc atcatggata agaagtacgc caagtgcctg cagaagatcg
     2881 acaaggacga tgtgaacggc aattacgaga agatcaacta taagctgctg cccggcccta
     2941 ataagatgct gccaaaggtg ttcttttcta agaagtggat ggcctactat aaccccagcg
     3001 aggacatcca gaagatctac aagaatggca cattcaagaa gggcgatatg tttaacctga
     3061 atgactgtca caagctgatc gacttcttta aggatagcat ctcccggtat ccaaagtggt
     3121 ccaatgccta cgatttcaac ttttctgaga cagagaagta taaggacatc gccggctttt
     3181 acagagaggt ggaggagcag ggctataagg tgagcttcga gtctgccagc aagaaggagg
     3241 tggataagct ggtggaggag ggcaagctgt atatgttcca gatctataac aaggactttt
     3301 ccgataagtc tcacggcaca cccaatctgc acaccatgta cttcaagctg ctgtttgacg
     3361 agaacaatca cggacagatc aggctgagcg gaggagcaga gctgttcatg aggcgcgcct
     3421 ccctgaagaa ggaggagctg gtggtgcacc cagccaactc ccctatcgcc aacaagaatc
     3481 cagataatcc caagaaaacc acaaccctgt cctacgacgt gtataaggat aagaggtttt
     3541 ctgaggacca gtacgagctg cacatcccaa tcgccatcaa taagtgcccc aagaacatct
     3601 tcaagatcaa tacagaggtg cgcgtgctgc tgaagcacga cgataacccc tatgtgatcg
     3661 gcatcgatag gggcgagcgc aatctgctgt atatcgtggt ggtggacggc aagggcaaca
     3721 tcgtggagca gtattccctg aacgagatca tcaacaactt caacggcatc aggatcaaga
     3781 cagattacca ctctctgctg gacaagaagg agaaggagag gttcgaggcc cgccagaact
     3841 ggacctccat cgagaatatc aaggagctga aggccggcta tatctctcag gtggtgcaca
     3901 agatctgcga gctggtggag aagtacgatg ccgtgatcgc cctggaggac ctgaactctg
     3961 gctttaagaa tagccgcgtg aaggtggaga agcaggtgta tcagaagttc gagaagatgc
     4021 tgatcgataa gctgaactac atggtggaca agaagtctaa tccttgtgca acaggcggcg
     4081 ccctgaaggg ctatcagatc accaataagt tcgagagctt taagtccatg tctacccaga
     4141 acggcttcat cttttacatc cctgcctggc tgacatccaa gatcgatcca tctaccggct
     4201 ttgtgaacct gctgaaaacc aagtatacca gcatcgccga ttccaagaag ttcatcagct
     4261 cctttgacag gatcatgtac gtgcccgagg aggatctgtt cgagtttgcc ctggactata
     4321 agaacttctc tcgcacagac gccgattaca tcaagaagtg gaagctgtac tcctacggca
     4381 accggatcag aatcttccgg aatcctaaga agaacaacgt gttcgactgg gaggaggtgt
     4441 gcctgaccag cgcctataag gagctgttca acaagtacgg catcaattat cagcagggcg
     4501 atatcagagc cctgctgtgc gagcagtccg acaaggcctt ctactctagc tttatggccc
     4561 tgatgagcct gatgctgcag atgcggaaca gcatcacagg ccgcaccgac gtggattttc
     4621 tgatcagccc tgtgaagaac tccgacggca tcttctacga tagccggaac tatgaggccc
     4681 aggagaatgc catcctgcca aagaacgccg acgccaatgg cgcctataac atcgccagaa
     4741 aggtgctgtg ggccatcggc cagttcaaga aggccgagga cgagaagctg gataaggtga
     4801 agatcgccat ctctaacaag gagtggctgg agtacgccca gaccagcgtg aagcacaaaa
     4861 ggccggcggc cacgaaaaag gccggccagg caaaaaagaa aaagggatcc tacccatacg
     4921 atgttccaga ttacgcttat ccctacgacg tgcctgatta tgcataccca tatgatgtcc
     4981 ccgactatgc cccggcagct aagagggtga aactggatgg cggtagcggc ggaggccccg
     5041 ccgccaagcg cgtgaagctg gacctcgagc accaccacca ccaccactga gatccggctg
     5101 ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat
     5161 aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat
     5221 ccggattggc gaatgggacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt
     5281 tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt
     5341 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc
     5401 tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga
     5461 tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc
     5521 cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt
     5581 ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct
     5641 gatttaacaa aaatttaacg cgaattttaa caaaatatta acgtttacaa tttcaggtgg
     5701 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
     5761 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
     5821 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
     5881 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
     5941 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
     6001 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
     6061 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
     6121 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
     6181 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
     6241 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
     6301 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
     6361 gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
     6421 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
     6481 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
     6541 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
     6601 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
     6661 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
     6721 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     6781 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     6841 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     6901 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     6961 gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta
     7021 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     7081 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     7141 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     7201 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     7261 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     7321 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     7381 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     7441 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     7501 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     7561 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
     7621 ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat
     7681 atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc
     7741 cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg
     7801 cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg
     7861 ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt
     7921 aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca
     7981 gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa
     8041 gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg
     8101 gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac
     8161 atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc
     8221 agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac
     8281 agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact
     8341 tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg
     8401 tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct
     8461 gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca
     8521 tgcgcacccg tggggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg
     8581 tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg
     8641 acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg
     8701 ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga
     8761 tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg
     8821 gtcgagatcc cggtgcctaa tgagtgagct aacttacatt aattgcgttg cgctcactgc
     8881 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
     8941 ggagaggcgg tttgcgtatt gggcgccagg gtggtttttc ttttcaccag tgagacgggc
     9001 aacagctgat tgcccttcac cgcctggccc tgagagagtt gcagcaagcg gtccacgctg
     9061 gtttgcccca gcaggcgaaa atcctgtttg atggtggtta acggcgggat ataacatgag
     9121 ctgtcttcgg tatcgtcgta tcccactacc gagatatccg caccaacgcg cagcccggac
     9181 tcggtaatgg cgcgcattgc gcccagcgcc atctgatcgt tggcaaccag catcgcagtg
     9241 ggaacgatgc cctcattc
//
